Costus afer leaf extract protects against testicle damage caused by cyclosporine A in adult male Wistar rats through an antioxidant mechanism

Andrologia ◽  
2020 ◽  
Vol 52 (5) ◽  
Author(s):  
Hala H.A. Khattab ◽  
Manal M.S. Mansoury
Keyword(s):  
Adult Male ◽  
Cyclosporine A ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Antioxidant Mechanism ◽  
Male Wistar Rats ◽  
Costus Afer

scholarly journals Phytochemical Evaluation and Acute Toxicity Study of Ethanol Leaf Extract of Acalypha wilkesiana

2021 ◽  
Vol 2 (8) ◽  
pp. 715-720
Author(s):  
Anthony Osamuyi Iyamu ◽  
Uwaifoh Akpamu ◽  
Karen Uwarobehi Iyamu
Keyword(s):  
Acute Toxicity ◽  
Adult Male ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Proximate Analysis ◽  
Qualitative And Quantitative ◽  
Benin City ◽  
Synthetic Drugs ◽  
Male Wistar Rats ◽  
Acalypha Wilkesiana

Increased curiosity on natural plant products has been raised due to problems of cost, unavailability, and after-effects of countless synthetic drugs. Worrisome, many plant-derived formulations lack phytochemically or toxicological screening. Hence, this study phytochemical and elemental screened the ethanolic leaf extract of Acalypha wilkesiana and as well as determining acute toxicity in adult male Wistar rats. The leaves were obtained in Benin City, Nigeria. Ethanol extraction was carried out on leaves and the extract was subjected to proximate, qualitative, and quantitative phytochemical screening and elemental analysis. Acute toxicity was determined on 12 adult male Wistar rats following Lork’s method. Proximate analysis revealed a high presence of carbohydrate, ash, fiber, and moisture. The qualitative and quantitative evaluation showed the abundance of alkaloids (68.7 ± 0.120%), flavonoids (34.7 ± 0.001%) and minute (<1mg/g) saponins, tannins, phenol, and terpenes. The extract contain nutritive (vitamin E = 1.184 ± 0.055µg/g; vitamin A = 0.0066 ± 0.003µg/g; vitamin C = 0.046 ± 0.037µg/g) and anti-nutritive (oxalates = 229.780 ± 16.93mg/100g; cyanide=0.162 ± 0.006 mg/100g; phytate = 0.131 ± 0.01mg/100g) elements. The elemental evaluation showed an abundance of potassium, sodium, and chloride with traces of cadmium and lead and the absence of manganese and copper. There was no sign of acute toxicity or mortality at an extract dose of 5000mg/kg. These findings indicate the ethanol leaf extract of A. wilkesiana as a rich source of phytochemicals and major macro elements and high safety at 5000mg/kg dose. Considering the several components in the leaves extract, Acalypha wilkesiana leaf might be pharmacological significant for the biological system.

scholarly journals Hepato-Renal Activities of Hydro-Methanol Leaf Extract of Cnidoscolus aconitifolius in Adult Male Wistar Rats

2021 ◽  
Vol 11 (4) ◽  
pp. 5-9
Author(s):  
Ijeoma Ezebuiro ◽  
Avweresuoghene Ododo ◽  
Uchechukwu Ifeanyichukwu Apugo
Keyword(s):  
Adult Male ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Negative Control ◽  
Protective Agent ◽  
Treatment Groups ◽  
Cnidoscolus Aconitifolius ◽  
Group I ◽  
Male Wistar Rats ◽  
Liver And Kidney

Introduction: Medicinal plants such as Cnidoscolus aconitifolius (CA) have been studied over the years for their protective and curative potentials against a myriad of common global health challenges such as hepatorenal injuries. Objectives: To ascertaining the effects of the hydromethanol leaf extract of CA (HMLECA) on hepato-renal parameters in adult male wistar rats. Method: A total of 18 adult male wistar rats were divided into 3 groups of six rats each. Group I served as the negative control which received distilled water while groups 2 and 3 served as extract treatment groups which received 200 and 400 mg/kg BW of the HMLECA respectively. The administration was daily for a period of 58 days while blood sample for the biochemical analysis was drawn via cardiac puncture at the end of the study following light chloroform anaesthesia. Results and Discussion: The results showed that the administration of both doses of the extract produced no significant (P<0.05) effect on the levels of serum liver enzyme (AST, ALT, ALP), TP, ALB, electrolytes (K+, Na+ and HCO3–), Creatinine and Urea while that of the 400 mg/kg BW produced significant (P<0.05) decrease in the level of TB and Cl –. Hence, the administration of the leaf extract in this study did not elicit any toxic effect on both the liver and kidney functions and could be effective as hepato-renal protective agent. Keywords: Cnidoscolus aconitifolius, Liver function, Liver enzymes, Renal function, Serum biochemicals, Serum electrolytes

Profertility effects of aqueous leaf extract of Telfairia occidentalis in adult male Wistar rats

2015 ◽  
Vol 14 (2) ◽  
pp. 88 ◽  
Author(s):  
SakpaLucky Christopher ◽  
Onovughakpo-SakpaOriseseyigbemi Ejuoghanran ◽  
OkhimamheAkhalumhe Festus
Keyword(s):  
Adult Male ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Telfairia Occidentalis ◽  
Male Wistar Rats ◽  
Aqueous Leaf Extract

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

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