Can Real-Time Tissue Elastography Be Used to Assess The Healing Process of Injured Superficial Digital Flexor Tendon in Horses?

2014 ◽  
Vol 46 ◽  
pp. 23-24
Author(s):  
N. Tamura ◽  
H. Asano ◽  
Y. Kasashima
2014 ◽  
Vol 27 (05) ◽  
pp. 358-365 ◽  
Author(s):  
P. R. Van Weeren ◽  
C. H. A. Van de Lest ◽  
J. Boere ◽  
M. Reyes ◽  
J. C. Ionita ◽  
...  

SummaryObjective: Even though equine multi-limb tendinopathy models have been reported, it is unknown if fore- and hindlimb tendon healing behave similarly. The aim of this study was to compare the healing process of surgically induced superficial digital flexor tendon (SDFT) core lesions of fore- and hindlimbs in horses.Methods: Tendon core lesions were surgically induced in the SDFT of both fore- and hindlimbs in eight horses. One randomly assigned forelimb and one randomly assigned hindlimb were injected with saline one and two weeks post-surgery. The healing process was monitored clinically and ultrasonographically. After 24 weeks, the tendons were harvested and biochemical, biomechanical and histological parameters were evaluated.Results: Twenty-four weeks post-surgery, the forelimb SDFT lesions had a significantly higher colour Doppler ultrasound vascularization score (p = 0.02) and glycosaminoglycan concentration (p = 0.04) and a significantly lower hydroxylysylpyridinoline content (p = 0.03).Clinical relevance: Our results indicate that fore- and hindlimb SDFT surgically induced lesions exhibit significant differences in several important parameters of tendon healing 24 weeks post-surgery. These differences create significant challenges in using all four limbs and accurately interpreting the results that one might generate. Therefore these findings do not support the use of four-limb models for study of tendon injury until the reasons for these differences are much better understood.


2020 ◽  
Vol 40 (01) ◽  
Author(s):  
Mohamed Mostafa ◽  
Ahmed , Adel Al-Akraa Khalil ◽  
Adel Al-Akraa

The aim of this study was to assess the effect of platelet rich plasma (PRP) on the healing of created core lesions in the superficial digital flexor tendon (SDFT) in donkeys. Serial histopathological specimens were taken from normal and at 5th, 15th, 30th, 60th and 90th days post PRP treatment. Histopathological findings showed early rapid healing process with early angiogenesis, increased fibroblastic condensation, the nuclei appeared more elongated, flattened arranged in the peripheral collagen fibers. The collagen fibers changed from randomly arranged to semi-parallel and finally highly reoriented parallel closely packed collagen fibers at 90th days of PRP treatment. Compared to saline control displayed few scattered fibroblasts and angiogenesis. As well as less regularly arranged collagen fibers. Histopathological evaluation 90 days after three successive PRP treatment proved a more rapid tendon tissue reconstruction and maturation similar to the healthy tendons.


2000 ◽  
Vol 5 (1) ◽  
Author(s):  
M.V.M. MACHADO ◽  
L.C. VULCANO ◽  
C.A. HUSSNI ◽  
A.L.G. ALVES

Foi analisado o efeito da laserterapia com emissão do raio laser arsenieto de gálio na reparação tendínea de eqüinos após indução de tendinite experimental. Foram utilizados 10 eqüinos, SRD, com idade média de 2 a 5 anos, selecionados quanto a normalidade do aparelho locomotor. A tendinite foi induzida pela injeção de colagenase C1639 Tipo I-S na concentração de 2,5 mg/ml na dose de 0,5 ml nos tendões flexores superficiais de ambos os membros anteriores. Após 48 horas da injeção da colagenase, foram realizados exames ultra-sonográficos consecutivos com intervalos de 24 horas, para avaliação das alterações ocorridas no tendão e instituído a laser terapia diária por 15 dias com emissão local do raio laser Arsenieto de Gallium na dose de 8 joules/cm² no membro anterior esquerdo, permanecendo o contra-lateral (direito) como controle. No 40º dia de avaliação ultra-sonográfica realizou-se biópsia na região da lesão para exames histológicos. Os exames ultrasonográfico e histológico não demonstraram diferenças entre os membros tratados e os membros controle. Esses resultados demonstraram que o raio laser Arsenieto de Gallium na dosimetria de 8 joules/cm² não interferiu de forma significativa no processo de reparação tendínea. Effects of laser therapy on experimental tendinitis in horses: ultrasonographic and histologic study Abstract Ultrasonography is being used very successfully for the evaluation of equine soft tissues, improving the diagnosis and monitoring soft tissue musculskeletal injury accurately and noninvasively. The superficial digital flexor tendon is by far the most frequently involved tendon in sport horse injury, being this way, highly adequate for ultrasonographic characterization and healing. Colagenase was injected bilaterally in the superficial digital flexor tendon, at the medium third metacarpus in ten horses. Ultrasonography was performed 24 hours later in order to study the effect if this inflammatory agent on the tendon ultrasongraphy was performed using a real time ultrasound with 7.5 MHz transducer. According to GENOVESE et al. (1986) classification, types II and III injuries were observed. One limb of each horse was treated with “soft laser” daily for 15 consecutive days using 8 joules/cm. The opposite limb was used as control. Ultrasonography was performed every 48 hours for 40 days showing no difference in the healing between treated and untreated limbs. No significant differences were observed between the histological aspects of the superficial digital flexor tendon healing and the ultrasonographic images. It was possible then to conclude that laser therapy did not interfere in the tendon healing.


2021 ◽  
Vol 8 (4) ◽  
pp. 54
Author(s):  
Daniele Serrani ◽  
Antonella Volta ◽  
Franco Cingolani ◽  
Luca Pennasilico ◽  
Caterina Di Bella ◽  
...  

Real-time elastosonography (RTE) is a recently described, non-invasive, ultrasonographic technique developed to assess tissue elasticity. The main aim of this study was to investigate the ultrasonographic and elastosonographic appearance of the common calcaneal tendon (CCT) in an ovine model, and to monitor the progression of tendon healing after an experimentally-induced tendinopathy. Sound tendons were initially evaluated (T0) with a caliper and by a single operator with ultrasound. Ultrasonographic and elastosonographic images were then acquired. Subsequently, ultrasound-guided tendon lesions were induced by injecting 500 IU of Type IA collagenases proximally to the calcaneal tuberosity. Caliper measurement, ultrasonography and elastosonography were then repeated at 15 (T1), 30 (T2) and 60 (T3) days. Clinically measured width of the tendon, ultrasonographic thickness and width and percentage of hard (Elx-t%hrd) and soft (Elx-t%sft) tissue were recorded. Statistical analysis was performed on the data collected; statistical significance was set at p < 0.05. Intra-class correlation coefficient (ICC) revealed good (0.68) repeatability of elastosonographic evaluation of the CCT. The tendon width was significantly increased when comparing T0 with T1–2 and decreased when comparing T1–2 with T3. Ultrasound-assessed thickness was significantly increased between T0–T1 and decreased between T1-T2–3. Elx-t%hrd was significantly decreased at T1–2–3 and Elx-t%sft was significantly increased at T1–2–3. In conclusion, the ovine CCT is a highly stiff structure that undergoes a severe loss of stiffness during the healing process. Thickness and width of the tendon increased during the first 30 days and then reduced progressively along the subsequent 30 days. Ultrasonographic appearance of the tendon remained severely abnormal and the tendon showed severely reduced elastic proprieties 60 days after lesion induction.


1992 ◽  
Vol 24 (1) ◽  
pp. 46-51 ◽  
Author(s):  
ANN E. KRAUS-HANSEN ◽  
G. E. FACKELMAN ◽  
C. BECKER ◽  
R. M. WILLIAMS ◽  
F. S. PIPERS

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