Comparing ITS‐2 and COI as DNA barcode markers for the most common cyathostomin species based on comparative phylogenetic analysis

In October 2020, a highly pathogenic avian influenza (HPAI) subtype H5N8 virus was identified from a fecal sample of a wild mandarin duck (Aix galericulata) in South Korea. We sequenced all eight genome segments of the virus, designated as A/Mandarin duck/Korea/K20-551-4/2020(H5N8), and conducted genetic characterization and comparative phylogenetic analysis to track its origin. Genome sequencing and phylogenetic analysis show that the hemagglutinin gene belongs to H5 clade 2.3.4.4 subgroup B. All genes share high levels of nucleotide identity with H5N8 HPAI viruses identified from Europe during early 2020. Enhanced active surveillance in wild and domestic birds is needed to monitor the introduction and spread of HPAI via wild birds and to inform the design of improved prevention and control strategies.

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Morphometry and Phylogeny of the Different Populations of Selaginella tamariscina (P. Beauv.) Spring and S. pulvinata (Hook. & Grev.) Maxim. in Northern Thailand

Chiang Mai University Journal of Natural Sciences ◽

10.12982/cmujns.2021.077 ◽

2021 ◽

Vol 20 (4) ◽

Author(s):

Udon Pongkawong ◽

◽

Jatupol Kampuansai ◽

Rossarin Pollawatn ◽

Arunothai Jampeetong ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Southern China ◽

Phylogenetic Analyses ◽

Dna Barcode ◽

Morphological Characteristics ◽

Taxonomic Status ◽

Morphological Characters ◽

The Philippines ◽

Northern Thailand ◽

Selaginella Tamariscina

Abstract “Dok Hin” is the Thai local name for Selaginella species that form rosettes. They commonly distributes in Siberia, Manchuria, southern China, Japan, the Philippines and Thailand. Morphology of Dok Hin is very resemble leading to misidentification. So, exactly number of species of Dok Hin in Thailand and their differences in morphological characteristics is not well understood. Thus, revision of morphological characters and phylogenetic confirmation of the taxonomic identification are needed. This study aims to examine morphological charateristics and phylogenetic patterns in eight populations of the Dok Hin in Northern Thailand. Morphology of Dok Hin from each populations was quantitatively examined using 15 vegetative and 6 reproductive characters meanwhile phylogenetic analyses was explored by DNA barcode ITS2. The results of the phylogenetic analysis revealed the existence of two species of Dok Hin, S. tamariscina and S. pulvinata. Selaginella tamariscina can be distinguished from S. pulvinata by its presence of a pseudotrunk above ground and ridges of dorsal leaves. On the other hand, the results of phylogenetic analysis indicated the differences among populations of S. pulvinata as well. Chiang Mai populations of S. pulvinata was characterized by peculiar set of characters long leaves and leaf apices look like caudate, while the rest of their populations have shorter leaves and leaf apices look like aristate. It indicates that S. pulvinata has genetic and phenotypic divergence among populations. However, additional studies of Dok Hin populations in other parts of Thailand and studies on different genetic markers are necessary to confirm the taxonomic status of S. pulvinata. Keywords: Dok Hin, Morphometric, Phylogeny, Pseudotrunk, Resurrection plant

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Flightless Disphaerona rediscovered in China: mtDNA phylogeography of the Yunnan clade and the sobering state of fungus weevil phylogenetics (Coleoptera: Anthribidae)

Fragmenta Entomologica ◽

10.4081/fe.2016.174 ◽

2016 ◽

Vol 48 (2) ◽

pp. 89 ◽

Cited By ~ 1

Author(s):

Vasily V. Grebennikov

Keyword(s):

Phylogenetic Analysis ◽

Dna Barcode ◽

Sister Group ◽

The State ◽

Female Genitalia ◽

Dabie Mountains ◽

Male And Female ◽

The Family

This paper reports discovery of the genus Disphaerona Jordan, 1902 in two widely separated regions in China: in three isolated highlands in Yunnan (the Cang Shan Range, Mt. Jizu and Mt. Haba) and in the Dabie Mountains of Hubei, at the border with Anhui. Till present the genus was known from China only from four specimens of D. chinensis (including its holotype) described from Sichuan in 1995. Sixteen specimens from each of the three newly detected localities in Yunnan were DNA barcoded and the data were made publicly available at dx.doi.org/10.5883/DS-DISPHAE. Temporal phylogenetic analysis revealed a topology suggesting monophyly and recent (0.2–0.4 Ma) origin of the populations on both Mt. Jizu and Mt. Haba. The population on the Cang Shan Range harbours representatives of both clades forming the ingroup, mtDNA of which diverged some 7.9 Ma. The habitus of the holotype of the only nominative Chinese Disphaerona species, D. chinensis, is illustrated, together with habitus and male and female genitalia of specimens newly discovered in both Yunnan and Hubei. All specimens of Disphaerona from Yunnan are seemingly conspecific and are taxonomically assigned to D. chinensis. Two similar males and one sympatric but dissimilar, smaller female from the Dabie Mountains do not have associated DNA barcode data. It remains unknown whether the three Dabie Mountains specimens are conspecific or even congeneric; pending this uncertainty they are tentatively treated as Disphaerona without a Linnaean name. Disphaerona population in the Dabie Mountains, if indeed a member of this hypothesised clade, forms the easternmost record of the genus. Monophyly and sister group of Disphaerona are briefly discussed and both are shown as unknown and unverifiable. A brief overview of the state of phylogenetic knowledge of Anthribidae is given, which is practically non-existing and with neither the family, nor any of its tribes and the vast majority of genera tested as monophyletic.

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