Modulation of aroma compounds in yeast and lactic acid bacterium co‐fermented pork hydrolysates by thermal treatment and addition of aroma precursors (cysteine and xylose)

Author(s):  
Xinzhi Li ◽  
Shao‐Quan Liu
2006 ◽  
Vol 188 (23) ◽  
pp. 8070-8078 ◽  
Author(s):  
Shinya Sugimoto ◽  
Hiroyuki Yoshida ◽  
Yoshimitsu Mizunoe ◽  
Keigo Tsuruno ◽  
Jiro Nakayama ◽  
...  

ABSTRACT In this study, we report the purification, initial structural characterization, and functional analysis of the molecular chaperone ClpB from the gram-positive, halophilic lactic acid bacterium Tetragenococcus halophilus. A recombinant T. halophilus ClpB (ClpB Tha ) was overexpressed in Escherichia coli and purified by affinity chromatography, hydroxyapatite chromatography, and gel filtration chromatography. As demonstrated by gel filtration chromatography, chemical cross-linking with glutaraldehyde, and electron microscopy, ClpB Tha forms a homohexameric single-ring structure in the presence of ATP under nonstress conditions. However, under stress conditions, such as high-temperature (>45°C) and high-salt concentrations (>1 M KCl), it dissociated into dimers and monomers, regardless of the presence of ATP. The hexameric ClpB Tha reactivated heat-aggregated proteins dependent upon the DnaK system from T. halophilus (KJE Tha ) and ATP. Interestingly, the mixture of dimer and monomer ClpB Tha , which was formed under stress conditions, protected substrate proteins from thermal inactivation and aggregation in a manner similar to those of general molecular chaperones. From these results, we hypothesize that ClpB Tha forms dimers and monomers to function as a holding chaperone under stress conditions, whereas it forms a hexamer ring to function as a disaggregating chaperone in cooperation with KJE Tha and ATP under poststress conditions.


2014 ◽  
Vol 23 (5) ◽  
pp. 1541-1550 ◽  
Author(s):  
Apinun Kanpiengjai ◽  
Wannisa Rieantrakoonchai ◽  
Ronachai Pratanaphon ◽  
Wasu Pathom-aree ◽  
Saisamorn Lumyong ◽  
...  

2021 ◽  
pp. 101502
Author(s):  
Fred Kwame Ofosu ◽  
Fazle Elahi ◽  
Eric Banan-Mwine Daliri ◽  
Sang-Ik Han ◽  
Deog-Hwan Oh

2017 ◽  
Vol 5 (30) ◽  
Author(s):  
Yanath Belguesmia ◽  
Valérie Leclère ◽  
Matthieu Duban ◽  
Eric Auclair ◽  
Djamel Drider

ABSTRACT We report the draft genome sequence of Enterococcus faecalis DD14, a strain isolated from meconium of a healthy newborn at Roubaix Hospital (France). The strain displayed antagonism against a set of Gram-positive bacteria through concomitant production of lactic acid and bacteriocin. The genome has a size of 2,893,365 bp and a 37.3% G+C ratio and is predicted to contain at least 2,755 coding sequences and 62 RNAs.


2011 ◽  
Vol 102 (7) ◽  
pp. 4827-4833 ◽  
Author(s):  
Paulraj Kanmani ◽  
R. Satish kumar ◽  
N. Yuvaraj ◽  
K.A. Paari ◽  
V. Pattukumar ◽  
...  

2013 ◽  
Vol 11 (4) ◽  
pp. 181-186 ◽  
Author(s):  
Yun-Seok Lee ◽  
Tae-Young Song ◽  
Won-Sik Kong ◽  
Min-Ho Yoon

Author(s):  
Cristobal A. Onetto ◽  
Peter J. Costello ◽  
Radka Kolouchova ◽  
Charlotte Jordans ◽  
Jane McCarthy ◽  
...  

Malolactic fermentation is an indispensable step in the elaboration of most wines and is generally performed by Oenococcus oeni , a Gram-positive heterofermentative lactic acid bacterium species. While O. oeni is tolerant to many of the wine stresses, including low pH and high ethanol concentrations, it has high sensitivity to SO 2 , an antiseptic and antioxidant compound regularly used in winemaking.


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