Ontogeny of the stomach in winter flounder: characterization and expression of the pepsinogen and proton pump genes and determination of pepsin activity

1999 ◽  
Vol 55 (5) ◽  
pp. 897-915 ◽  
Author(s):  
S. E. Douglas ◽  
A. Gawlicka ◽  
S. Mandla ◽  
J. W. Gallant
Keyword(s):  
Proton Pump ◽  
Winter Flounder ◽  
Pepsin Activity

scholarly journals New Liquid Chromatographic Method for Determination of Rabeprazole Sodium in Coated Tablets

2004 ◽  
Vol 87 (4) ◽  
pp. 842-846 ◽  
Author(s):  
Cássia V Garcia ◽  
Clésio S Paim ◽  
Martin Steppe
Keyword(s):  
Quantitative Determination ◽  
Proton Pump ◽  
Chromatographic Method ◽  
Array Detector ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Validation Parameters ◽  
Rabeprazole Sodium ◽  
Liquid Chromatographic

Abstract Rabeprazole sodium is a proton pump inhibitor that covalently binds and inactivates the gastric parietal cell proton pump (H+/K+ ATPase). Little has been published about the quantitative determination of this drug. The aim of this research was to develop a new liquid chromatographic method for quantitative determination of rabeprazole in coated tablets. The system consisted of a Hypersil Keystone Betabasic C8 column (250 × 4.6 mm, 5 μm particle size), an isocratic acetonitrile–water (35 + 65) mobile phase at a flow rate of 1.0 mL/min, and a diode array detector set at 282 nm. The following validation parameters were evaluated: linearity, precision, accuracy, specificity, detection and quantitation limits, and robustness. The method showed good linearity in the concentration range of 10–70 μg/mL. The quantitation limit was 2.43 μg/mL, and the detection limit was 0.80 μg/mL. The intra- and interday precision data showed that the method has good reproducibility (relative standard deviation = 1.03). Accuracy and robustness were also evaluated, and the results were satisfactory. The mean recovery was 101.61%. The analysis of a placebo mixture demonstrated the method is also specific.

A fluorimetric method for the determination of pepsin activity

2003 ◽  
Vol 316 (1) ◽  
pp. 11-14 ◽  
Author(s):  
Roseli Aparecida da Silva Gomes ◽  
Rodrigo Pedutti Batista ◽  
Andréia Costa de Almeida ◽  
Daurin Narciso da Fonseca ◽  
Luis Juliano ◽  
...  
Keyword(s):  
Pepsin Activity ◽  
Fluorimetric Method

Electron Crystallographic Determination of the Structure of Bacteriorhodopsin

1990 ◽  
Vol 48 (1) ◽  
pp. 90-91
Author(s):  
R. Henderson ◽  
J.M. Baldwin ◽  
T.A. Ceska ◽  
E. Beckman ◽  
F. Zemlin ◽  
...  
Keyword(s):  
High Resolution ◽  
Proton Pump ◽  
Three Dimensional ◽  
Atomic Resolution ◽  
Two Dimensional ◽  
New Methods ◽  
Density Map ◽  
Diffraction Patterns

The light driven proton pump bacteriorhodopsin (bR) occurs naturally as two-dimensional crystals. A three-dimensional density map of the structure, at near atomic resolution, has been obtained by studying the crystals using electron cryo-microscopy to obtain diffraction patterns and high resolution micrographs (1).New methods have been developed for analysing micrographs from tilted specimens, incorporating the methods previously developed for untilted specimens that enable large areas to be analysed and corrected for distortions. Data from 72 images, from both tilted and untilted specimens, have been analysed to produce the phases of 2700 independent Fourier components of the structure. The amplitudes of these components have been accurately measured from 150 diffraction patterns. Together, these data represent about half of the full three-dimensional transform to 3.5 Å. The distribution of the data which is included in the map is shown in fig. 1. For specimen tilts up to around 20° the data is essentially complete. For higher tilts the data is more sparsely sampled, and is at present about half complete.

Ontogeny of pepsinogen and gastric proton pump expression in red porgy (Pagrus pagrus): Determination of stomach functionality

Aquaculture ◽  
2007 ◽  
Vol 270 (1-4) ◽  
pp. 369-378 ◽  
Author(s):  
María J. Darias ◽  
Harry M. Murray ◽  
Jeffrey W. Gallant ◽  
Susan E. Douglas ◽  
Manuel Yúfera ◽  
...  
Keyword(s):  
Proton Pump ◽  
Red Porgy ◽  
Gastric Proton Pump ◽  
Pagrus Pagrus

scholarly journals AN LC- MS/MS METHOD FOR THE DETERMINATION OF OMEPRAZOLE ON PROTON PUMP INHIBITOR IN HUMAN PLASMA

2016 ◽  
Vol 27 (2) ◽  
pp. 80
Author(s):  
T. Sudha ◽  
Kalan Kumar Reddy ◽  
P. V. Hemalatha ◽  
V. R. Ravikumar
Keyword(s):  
Proton Pump Inhibitor ◽  
Human Plasma ◽  
Proton Pump

scholarly journals Chronopharmacology and drug prescribing pattern of physicians in a tertiary care hospital of North India

2018 ◽  
Vol 7 (3) ◽  
pp. 499
Author(s):  
Diwanshu Sharma ◽  
Pavan Malhotra
Keyword(s):  
Proton Pump ◽  
Beta Blockers ◽  
Accurate Determination ◽  
Tertiary Care ◽  
Drug Effects ◽  
North India ◽  
Care Hospital ◽  
Prescribing Pattern ◽  
Physician Prescribing

Background: Chronopharmacology is the science dealing with the optimization of drug effects and the minimization of adverse effects by timing medication in relation to the biological rhythm. This concept came into picture to make us understand about periodic and predictable changes in both desired effects and tolerance of medication Chronotherapeutics approach gives more accurate determination of the time when patients are at highest risk and in greatest need of therapy. This Chronopharmacological principle is used in the therapy of various diseases like cardiovascular diseases, allergy and many more. The objective of the study was to analyse whether chronopharmacological approach was being applied in clinical practice by comparing chronopharmacology of 4 drugs to their prescribing pattern by the physicians.Methods: This was observational study where 700 prescriptions written by physicians were audited. To study all the drugs would have been tedious so four commonly used drugs were chosen that is, Proton pump inhibitors, statins, Beta blockers and subcutaneous Insulin.Results: Results showed that chronopharmacological approach has been applied in the clinical field though physician didn’t have clear concept about chronopharmacology and it is relation to the prescribing. Despite this, maximum physician prescribing pattern was comparable to the chronopharmacological data except in case of Proton pump inhibitors.Conclusions: It is concluded that Chronotherapeutics approach gives more accurate determination of the time when patients are at highest risk and in greatest need of therapy. Nevertheless, this variation is only seldom considered by clinicians. If drugs are prescribed by following chronopharmacological approach it can prove beneficial to the patients as more of effectiveness of the drug and lesser side effects.

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