Protective effect of granulocyte-colony stimulating factor against amphotericin B-induced myelosuppression in vitro

Concomitant use of allogeneic donor granulocyte transfusions and amphotericin B in febrile neutropenic recipients may be limited by the increased incidence of respiratory distress. In vitro effects of amphotericin B and AmBisome were compared on polymorphonuclear leukocyte (PMN) aggregation from PMNs isolated from granulocyte–colony-stimulating factor (G-CSF)/dexamethasone–mobilized allogeneic donors. Six allogeneic donors were mobilized with G-CSF (600 μg subcutaneously) and dexamethasone (8 mg orally) 12 hours before leukopheresis. AmBisome was associated with significantly less PMN aggregation (100 μM [μg/mL]) (0.33% ± 0.33% vs 54.33% ± 5.82%; P < .001) than amphotericin B. Furthermore, with the addition of the PMN agonist, FMLP, AmBisome was also associated with significantly less aggregation (100 μM [μg/mL]) (18.67% ± 1.45% vs 54.67% ± 2.4%;P < .001). In summary, these studies demonstrate that liposomal amphotericin is associated with significantly less in vitro PMN aggregation than amphotericin B and could possibly be administered concomitantly with mobilized allogeneic PMN infusions.

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Faculty Opinions recommendation of A randomized clinical trial of endometrial perfusion with granulocyte colony-stimulating factor in in vitro fertilization cycles: impact on endometrial thickness and clinical pregnancy rates.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.718238660.793490316 ◽

2014 ◽

Author(s):

Raoul Orvieto

Keyword(s):

Clinical Trial ◽

In Vitro Fertilization ◽

Endometrial Thickness ◽

Clinical Pregnancy ◽

Granulocyte Colony Stimulating Factor ◽

Colony Stimulating Factor ◽

Vitro Fertilization ◽

Clinical Pregnancy Rates ◽

Stimulating Factor

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Human Milk as an Enteral Source of Granulocyte Colony-Stimulating Factor (G-CSF): 1) Concentrations in Preterm and Term Milk, 2) G-CSF Survival after In Vitro Simulations of Digestion, and 3) Expression of G-CSF Receptor (G-CSF-R) in Human Fetal Intestine

Pediatric Research ◽

10.1203/00006450-199904020-01114 ◽

1999 ◽

Vol 45 (4, Part 2 of 2) ◽

pp. 187A-187A

Author(s):

Darlene A Calhoun ◽

Mathilde Lunoe ◽

Yan Du ◽

Robert D Christensen

Keyword(s):

Human Milk ◽

Granulocyte Colony Stimulating Factor ◽

Colony Stimulating Factor ◽

Stimulating Factor ◽

Fetal Intestine ◽

Factor G

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G-CSF receptor activation of the Src kinase Lyn is mediated by Gab2 recruitment of the Shp2 phosphatase

Blood ◽

10.1182/blood-2009-12-261636 ◽

2011 ◽

Vol 118 (4) ◽

pp. 1077-1086 ◽

Cited By ~ 25

Author(s):

Muneyoshi Futami ◽

Quan-sheng Zhu ◽

Zakary L. Whichard ◽

Ling Xia ◽

Yuehai Ke ◽

...

Keyword(s):

Tyrosine Kinases ◽

Receptor Tyrosine Kinases ◽

Src Kinase ◽

Receptor Activation ◽

Phosphorylation Sites ◽

Granulocyte Colony Stimulating Factor ◽

Colony Stimulating Factor ◽

Stimulating Factor ◽

In Cells

Abstract Src activation involves the coordinated regulation of positive and negative tyrosine phosphorylation sites. The mechanism whereby receptor tyrosine kinases, cytokine receptors, and integrins activate Src is not known. Here, we demonstrate that granulocyte colony-stimulating factor (G-CSF) activates Lyn, the predominant Src kinase in myeloid cells, through Gab2-mediated recruitment of Shp2. After G-CSF stimulation, Lyn dynamically associates with Gab2 in a spatiotemporal manner. The dephosphorylation of phospho-Lyn Tyr507 was abrogated in Shp2-deficient cells transfected with the G-CSF receptor but intact in cells expressing phosphatase-defective Shp2. Auto-phosphorylation of Lyn Tyr396 was impaired in cells treated with Gab2 siRNA. The constitutively activated Shp2E76A directed the dephosphorylation of phospho-Lyn Tyr507 in vitro. Tyr507 did not undergo dephosphorylation in G-CSF–stimulated cells expressing a mutant Gab2 unable to bind Shp2. We propose that Gab2 forms a complex with Lyn and after G-CSF stimulation, Gab2 recruits Shp2, which dephosphorylates phospho-Lyn Tyr507, leading to Lyn activation.

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Granulocyte-colony stimulating factor promotes invasion by human lung cancer cell lines in vitro

Clinical & Experimental Metastasis ◽

10.1007/bf00123394 ◽

1996 ◽

Vol 14 (4) ◽

pp. 351-357 ◽

Cited By ~ 7

Author(s):

Xin-Hai Pei ◽

Yoichi Nakanishi ◽

Koichi Takayama ◽

Jun Yatsunami ◽

Feng Bai ◽

...

Keyword(s):

Lung Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Human Lung ◽

Human Lung Cancer ◽

Granulocyte Colony Stimulating Factor ◽

Colony Stimulating Factor ◽

Lung Cancer Cell ◽

Stimulating Factor

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Granulocyte Colony-Stimulating Factor and an In Vitro Whole Blood Model of Melioidosis

European Journal of Clinical Microbiology & Infectious Diseases ◽

10.1007/s10096-003-1088-y ◽

2004 ◽

Vol 23 (3) ◽

pp. 205-207 ◽

Cited By ~ 3

Author(s):

A. C. Cheng ◽

P. Dasari ◽

B. J. Currie

Keyword(s):

Whole Blood ◽

Granulocyte Colony Stimulating Factor ◽

Colony Stimulating Factor ◽

Stimulating Factor

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Granulocyte colony-stimulating factor protects cardiac mitochondria in the early phase of cardiac injury

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.00774.2008 ◽

2009 ◽

Vol 296 (3) ◽

pp. H823-H832 ◽

Cited By ~ 15

Author(s):

Yoshimi Hiraumi ◽

Eri Iwai-Kanai ◽

Shiro Baba ◽

Yoshihiro Yui ◽

Yuri Kamitsuji ◽

...

Keyword(s):

Diastolic Function ◽

Early Phase ◽

Cardiac Injury ◽

Left Ventricular ◽

Cardiac Mitochondria ◽

Granulocyte Colony Stimulating Factor ◽

Colony Stimulating Factor ◽

Stimulating Factor ◽

Cellular Fate

Although granulocyte colony-stimulating factor (G-CSF) reportedly plays a cardioprotective role in several models of cardiac injury, clinical use of this drug in cardiac patients has been controversial. Here, we tested, in vivo and in vitro, the effect of G-CSF on cardiac mitochondria, which play a key role in determining cardiac cellular fate and function. Mild stimulation of C57/BL6 mice with doxorubicin (Dox) did not induce cardiac apoptosis or fibrosis but did induce damage to mitochondrial organization of the myocardium as observed through an electron microscope. Cardiac catheterization and echocardiography revealed that Dox did not alter cardiac systolic function or left ventricular size but did reduce diastolic function, an early sign of cardiac damage. Treatment with G-CSF attenuated significantly the damage to mitochondrial organization and rescued diastolic function. In an in vitro model for rat neonatal cardiomyocytes, a subapoptotic dose of Dox induced severe mitochondrial damage, including marked swelling of the cardiac mitochondria and/or decreased mitochondrial membrane potential. These mitochondrial changes were completely blocked by pretreatment with G-CSF. In addition, G-CSF dramatically improved ATP generation, which rescued Dox-impaired mitochondrial electron transport and oxygen consumption mainly through complex IV. These findings clearly indicate that G-CSF protects cardiac mitochondria, which are key organelles in the determination of cardiac cellular fate, in the early phase of cardiac injury.

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The incidence rate of unresponsive thin endometrium in frozen embryo transfer cycles: A case-series of therapy with granulocyte colony stimulating factor

International Journal of Reproductive BioMedicine ◽

10.18502/ijrm.v17i12.5797 ◽

2019 ◽

Cited By ~ 1

Author(s):

Shokouhosadat Miralaei ◽

Mahnaz Ashrafi ◽

Arezoo Arabipoor ◽

Zahra Zolfaghari ◽

Saeideh Taghvaei

Keyword(s):

Embryo Transfer ◽

Endometrial Thickness ◽

Case Series ◽

Frozen Embryo Transfer ◽

Granulocyte Colony Stimulating Factor ◽

Colony Stimulating Factor ◽

Cross Sectional ◽

Thin Endometrium ◽

Stimulating Factor

Background: Treatment-resistant thin endometrium (TTE) during in-vitro fertilization is a relatively uncommon and challenging problem. Objective: The primary aim of the study was to assess the TTE rate during frozen embryo transfer (FET) cycles and the secondary aim was to evaluate the effect of intrauterine instillation of granulocyte colony stimulating factor (G-CSF) in these cases. Materials and Methods: In this cross-sectional study, all of the women who underwent FET cycles with hormonal endometrial preparation in Royan Institute from June 2015 to March 2018 were evaluated and all of the cases with TTE diagnosis (endometrial thickness < 7 mm after using high doses of estradiol) were included. In the eligible cases, 300 μgr of G-CSF was infused intrauterine. If the endometrium had not reached at least a 7-mm, a second infusion was prescribed within 48 hr later. Results: During the study, 8,363 of FET cycles were evaluated and a total of 30 infertile patients (0.35%) with TTE diagnosis were detected. Finally, 20 eligible patients were included. The changes of endometrial thickness after G-CSF therapy were significant (p< 0.001); however, the endometrial thickness did not reach 7 mm in nine patients (45%) and the embryo transfer was canceled. Conclusion: It was found that the rate of TTE during the FET cycle is very low and intrauterine perfusion of G-CSF has a potential effect to increase the endometrial thickness in these patients; however, the rate of cancellation was still high and poor pregnancy outcomes were observed. Key words: Granulocyte colony-stimulating factor, Cryopreservation, Embryo transfer, Endometrial diseases.

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