scholarly journals Lysis of human macrophages by cytolytic CD4+ T cells fails to affect survival of intracellular Mycobacterium bovis-bacille Calmette-Guérin (BCG)

2008 ◽  
Vol 99 (1) ◽  
pp. 82-89 ◽  
Author(s):  
N. FAZAL ◽  
D. A. LAMMAS ◽  
M. RAHELU ◽  
A. D. PITHIE ◽  
J. S. H. GASTON ◽  
...  
Keyword(s):  
T Cells ◽  
Mycobacterium Bovis ◽  
Cd4 T Cells ◽  
Bacille Calmette Guerin ◽  
Human Macrophages

scholarly journals Macrophage-dependent Apoptosis of CD4+ T Lymphocytes from HIV-infected Individuals Is Mediated by FasL and Tumor Necrosis Factor

1997 ◽  
Vol 185 (1) ◽  
pp. 55-64 ◽  
Author(s):  
Andrew D. Badley ◽  
David Dockrell ◽  
Margaret Simpson ◽  
Ron Schut ◽  
David H. Lynch ◽  
...  
Keyword(s):  
T Cells ◽  
Tumor Necrosis Factor ◽  
T Lymphocytes ◽  
Cd4 T Cells ◽  
Tumor Necrosis ◽  
Human Macrophages ◽  
Infected Cells ◽  
Induced Apoptosis ◽  
Antigen Presenting ◽  
Necrosis Factor

Apoptosis of bystander uninfected CD4+ T lymphocytes by neighboring HIV-infected cells is observed in cell culture and in lymphoid tissue of HIV-infected individuals. This study addresses whether antigen-presenting cells such as human macrophages mediate apoptosis of CD4+ T cells from HIV-infected individuals. Uninfected human macrophages, and to a larger degree, HIV-infected macrophages mediate apoptosis of T cells from HIV-infected, but not from uninfected control individuals. This macrophage-dependent killing targets CD4+, but not CD8+ T lymphocytes from HIV-infected individuals, and direct contact between macrophages and lymphocytes is required. Additional analyses indicated that the apoptosis-inducing ligands, FasL and tumor necrosis factor (TNF), mediate this macrophage-induced apoptosis of CD4+ T cells. These results support a role for macrophage-associated FasL and TNF in the selective depletion of CD4+ T cells in HIV-infected individuals.

scholarly journals Priming by DNA Immunization Augments Protective Efficacy of Mycobacterium bovis Bacille Calmette-Guerin against Tuberculosis

2001 ◽  
Vol 69 (6) ◽  
pp. 4174-4176 ◽  
Author(s):  
Carl G. Feng ◽  
Umaimainthan Palendira ◽  
Caroline Demangel ◽  
Joanne M. Spratt ◽  
Adam S. Malin ◽  
...  
Keyword(s):  
T Cells ◽  
Mycobacterium Tuberculosis ◽  
Mycobacterium Bovis ◽  
Protective Efficacy ◽  
Mycobacterial Antigen ◽  
Dna Immunization ◽  
Bacille Calmette Guerin

ABSTRACT Sequential immunization with mycobacterial antigen Ag85B-expressing DNA and Mycobacterium bovis bacille Calmette-Guerin (BCG) was more effective than BCG immunization in protecting againstMycobacterium tuberculosis infection. Depletion of the CD8+ T cells in the immunized mice impaired protection in their spleens, indicating that this improved efficacy was partially mediated by CD8+ T cells.

scholarly journals ATP and Control of Intracellular Growth of Mycobacteria by T Cells

2002 ◽  
Vol 70 (11) ◽  
pp. 6456-6459 ◽  
Author(s):  
David H. Canaday ◽  
Reza Beigi ◽  
Richard F. Silver ◽  
Clifford V. Harding ◽  
W. Henry Boom ◽  
...  
Keyword(s):  
T Cells ◽  
Mycobacterium Bovis ◽  
Extracellular Atp ◽  
Intracellular Growth ◽  
Human Macrophages ◽  
Bystander Cells ◽  
And Control

ABSTRACT Extracellular ATP at millimolar concentrations inhibits growth of mycobacteria in human macrophages. Whether T cells can produce sufficient ATP is unknown. CD4+ and CD8+ T cells did not release sufficient ATP through either degranulation or lysis of bystander cells to restrict growth of Mycobacterium bovis BCG in monocytes.

Mycobacterium bovis and BCG induce different patterns of cytokine and chemokine production in dendritic cells and differentiation patterns in CD4+ T cells

Microbiology ◽  
2013 ◽  
Vol 159 (Pt_2) ◽  
pp. 366-379 ◽  
Author(s):  
Xiansong Zhang ◽  
Shuai Li ◽  
Yu Luo ◽  
Yingyu Chen ◽  
Shi Cheng ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Mycobacterium Bovis ◽  
Cd4 T Cells ◽  
Chemokine Production

scholarly journals Adiponectin Induces Pro-inflammatory Programs in Human Macrophages and CD4+T Cells

2012 ◽  
Vol 287 (44) ◽  
pp. 36896-36904 ◽  
Author(s):  
Xiang Cheng ◽  
Eduardo J. Folco ◽  
Koichi Shimizu ◽  
Peter Libby
Keyword(s):  
T Cells ◽  
Cd4 T Cells ◽  
Human Macrophages

scholarly journals Upregulation of Cytokines and Differentiation of Th17 and Treg by Dendritic Cells: Central Role of Prostaglandin E2 Induced by Mycobacterium bovis

2020 ◽  
Vol 8 (2) ◽  
pp. 195 ◽  
Author(s):  
Han Liu ◽  
Xuekai Xiong ◽  
Wenjun Zhai ◽  
Tingting Zhu ◽  
Xiaojie Zhu ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Prostaglandin E2 ◽  
Mycobacterium Bovis ◽  
Cd4 T Cells ◽  
Th17 Cell ◽  
Critical Role ◽  
Protein Detection ◽  
Cox Inhibitor ◽  
Cox 2

Mycobacterium bovis (M. bovis) is a zoonotic pathogen that causes bovine and human tuberculosis. Dendritic cells play a critical role in initiating and regulating immune responses by promoting antigen-specific T-cell activation. Prostaglandin E2 (PGE2)-COX signaling is an important mediator of inflammation and immunity and might be involved in the pathogenesis of M. bovis infection. Therefore, this study aimed to reveal the character of PGE2 in the differentiation of naïve CD4+ T cells induced by infected dendritic cells (DCs). Murine bone marrow-derived DCs were pre-infected with M. bovis and its attenuated strain M. bovis bacillus Calmette-Guérin (BCG). Then, the infected DCs were co-cultured with naïve CD4+ T cells with or without the cyclooxygenase (COX) inhibitor indomethacin. Quantitative RT-PCR analysis and protein detection showed that PGE2/COX-2 signaling was activated, shown by the upregulation of PGE2 production as well as COX-2 and microsomal PGE2 synthase (mPGES1) transcription in DCs specifically induced by M. bovis and BCG infection. The further co-culture of infected DCs with naïve CD4+ T cells enhanced the generation of inflammatory cytokines IL-17 and IL-23, while indomethacin suppressed their production. Following this, the differentiation of regulatory T cells (Treg) and Th17 cell subsets was significantly induced by the infected DCs rather than uninfected DCs. Meanwhile, M. bovis infection stimulated significantly higher levels of IL-17 and IL-23 and the differentiation of Treg and Th17 cell subsets, while BCG infection led to higher levels of TNF-α and IL-12, but lower proportions of Treg and Th17 cells. In mice, M. bovis infection generated more bacterial load and severe abnormalities in spleens and lungs, as well as higher levels of COX-2, mPGE2 expression, Treg and Th17 cell subsets than BCG infection. In conclusion, PGE2/COX-2 signaling was activated in DCs by M. bovis infection and regulated differentiation of Treg and Th17 cell subsets through the crosstalk between DCs and naive T cells under the cytokine atmosphere of IL-17 and IL-23, which might contribute to M. bovis pathogenesis in mice.

Mycobacterium bovis BCG vaccination induces memory CD4+ T cells characterized by effector biomarker expression and anti-mycobacterial activity

Vaccine ◽  
2007 ◽  
Vol 25 (50) ◽  
pp. 8384-8394 ◽  
Author(s):  
Janice J. Endsley ◽  
Alison Hogg ◽  
Lis J. Shell ◽  
Martin McAulay ◽  
Tracey Coffey ◽  
...  
Keyword(s):  
T Cells ◽  
Mycobacterium Bovis ◽  
Cd4 T Cells ◽  
Mycobacterium Bovis Bcg ◽  
Bcg Vaccination ◽  
Memory Cd4 T Cells

scholarly journals Efficacy and immunogenicity of different BCG doses in BALB/c and CB6F1 mice when challenged with H37Rv or Beijing HN878

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Bhagwati Khatri ◽  
James Keeble ◽  
Belinda Dagg ◽  
Daryan A. Kaveh ◽  
Philip J. Hogarth ◽  
...  
Keyword(s):  
Animal Model ◽  
T Cells ◽  
Body Weight ◽  
Cd4 T Cells ◽  
Significant Protection ◽  
Bacille Calmette Guerin ◽  
Preclinical Animal Model ◽  
Human Dose ◽  
Aerosol Infection

AbstractTwo strains of mice (BALB/c and CB6F1) were vaccinated with a range of Bacille Calmette-Guérin (BCG) Danish doses from 3 × 105 to 30 CFU/mouse, followed by aerosol infection with Mtb (H37Rv or West-Beijing HN878 strain). The results indicated that both strains of mice when infected with HN878 exhibited significant protection in their lungs with BCG doses at 3 × 105—3000 CFU (BALB/c) and 3 × 105—300 CFU (CB6F1). Whereas, a significant protection was seen in both strains of mice with BCG doses at 3 × 105—300 CFU when infected with H37Rv. A significant increase in the frequencies of BCG-specific IFNγ+ IL2+ TNFα+ CD4 T cells in the BCG doses at 3 × 105—3000 CFU (BALB/c) and 3 × 105—300 CFU (CB6F1) was seen. The IFNγ+ IL2+ TNFα+ CD4 T cells correlated with the Mtb burden in the lungs of HN878 infected mice (BALB/c and CB6F1) whereas, IFNγ+ TNFα+ CD4 T cells correlated with the BALB/c mice infected with H37Rv or HN878. The BCG dose at 3000 CFU (an equivalent single human dose in the mice by body weight) is protective in both strains of mice infected with H37Rv or HN878 and may serve an interesting dose to test new TB vaccine in a preclinical animal model.

Sign in / Sign up

Export Citation Format

Share Document