Heterotrophic bacterial populations in the mineral waters of thermal springs in Spain

1994 ◽  
Vol 77 (4) ◽  
pp. 370-381 ◽  
Author(s):  
María de los Angeles Mosso ◽  
María del Carmen de la Rosa ◽  
Carmen Vivar ◽  
María del Rosario Medina
2020 ◽  
Vol 8 (11) ◽  
pp. 1677
Author(s):  
Hugo Gildardo Castelán-Sánchez ◽  
Pablo M. Meza-Rodríguez ◽  
Erika Carrillo ◽  
David I. Ríos-Vázquez ◽  
Arturo Liñan-Torres ◽  
...  

Terrestrial thermal springs are widely distributed globally, and these springs harbor a broad diversity of organisms of biotechnological interest. In Mexico, few studies exploring this kind of environment have been described. In this work, we explore the microbial community in Chignahuapan hot springs, which provides clues to understand these ecosystems’ diversity. We assessed the diversity of the microorganism communities in a hot spring environment with a metagenomic shotgun approach. Besides identifying similarities and differences with other ecosystems, we achieved a systematic comparison against 11 metagenomic samples from diverse localities. The Chignahuapan hot springs show a particular prevalence of sulfur-oxidizing bacteria from the genera Rhodococcus, Thermomonas, Thiomonas, Acinetobacter, Sulfurovum, and Bacillus, highlighting those that are different from other recovered bacterial populations in circumneutral hot springs environments around the world. The co-occurrence analysis of the bacteria and viruses in these environments revealed that within the Rhodococcus, Thiomonas, Thermonas, and Bacillus genera, the Chignahuapan samples have specific species of bacteria with a particular abundance, such as Rhodococcus erytropholis. The viruses in the circumneutral hot springs present bacteriophages within the order Caudovirales (Siphoviridae, Myoviridae, and Podoviridae), but the family of Herelleviridae was the most abundant in Chignahuapan samples. Furthermore, viral auxiliary metabolic genes were identified, many of which contribute mainly to the metabolism of cofactors and vitamins as well as carbohydrate metabolism. Nevertheless, the viruses and bacteria present in the circumneutral environments contribute to the sulfur cycle. This work represents an exhaustive characterization of a community structure in samples collected from hot springs in Mexico and opens opportunities to identify organisms of biotechnological interest.


1994 ◽  
Vol 40 (1) ◽  
pp. 72-77 ◽  
Author(s):  
Ana-Cristina Ferreira ◽  
Paula Vasconcellos Morais ◽  
Milton S. da Costa

The quantitative variation in the microflora in bottled mineral waters stored in polyvinyl chloride bottles was studied immediately after bottling and after 7 days storage, over 9 months. The microflora was determined by combining total counts using ethidium bromide, and the number of iodonitrophenyltetrazolium (INT)-positive cells determined with heterotrophic plate counts on R2A for prolonged incubation periods at 22 and 37 °C. Immediately after bottling of mineral water A, the total counts with ethidium bromide varied between 70.0 and 495.0 bacteria/mL. The number of INT-positive bacteria varied between 14.0 and 40.0% counts. The heterotrophic plate counts were higher at 22 °C than at 37 °C and varied between 1.1 and 13.2% and between 0.7 and 9.3% of the total counts, respectively. After 7 days storage, total bacterial counts increased by 1000 times, indicating multiplication of bacteria that were present in low numbers immediately after bottling. The percentage of INT-positive bacteria remained between 2.6 and 39.0% of the total counts but the number of culturable bacteria was higher than the number of INT-positive cells. Similar counts were found in other mineral waters (B, C, and D), but the numbers of INT-positive cells in two of them (B and D) were higher than the heterotrophic plate counts at 22 °C. These results show the presence in the aquifer and (or) bottling system of a flora that is neither INT-positive nor culturable and reinforce previous results that still mineral waters have a large bacterial population after storage.Key words: mineral water, bacterial populations, ethidium bromide, INT, HPC.


1836 ◽  
Vol 126 ◽  
pp. 571-616 ◽  

If the chemistry of mineral waters has been as yet prosecuted to a very limited extent, notwithstanding the number of eminent analysts who have engaged in the research, much more has every other topic connected with their origin and nature been superficially treated. The characters of springs of every kind are so important as to deserve minute and laborious research; and notwithstanding the partial essays of Von Buch and others, the whole subject remains in a state of confusion, and is involved in incongruities and contradictions. The chief point to which the observations about to be described were directed, is the temperature of thermal springs; and, referring to this point alone, we might prove the almost total absence of exact data on the subject. Every traveller, to be sure, has measured the temperature of springs, but few have been aware of the difficulties which even this apparently simple inquiry involves. We should have considered the accurate determination of the temperatures of thermal springs the first step towards a theory of their production. The constancy of that temperature from day to day, from year to year, from century to century, would appear one of the most essential facts to determine; yet I am aware of scarcely a single published observation capable of being satisfactorily employed in such an inquiry. Not only are the errors of the instruments unknown, but the circumstances under which the observations are made are liable to perpetual change. For instance, a spring in the state of nature may rise from rock directly, or from amongst debris. In the latter case, to fix the temperature is difficult, because it varies at different points; and it is nearly useless, because a year hence the circumstaqces of its efflux may be wholly changed. Again, in the more usual case of thermal waters being medicinally employed, it is frequently impossible (at least without much trouble) to reach the true source of the water, which is carried through pipes, conduits, and reservoirs before it is finally employed; and in this case the temperature is usually taken at the bath-cock, or at the 'buvette,’ or drinking-cock, where consequently the water has been subjected to the variable cooling action of its intermediate transit. Thus, for example, at the great establishment of La Raillière at Cauteretz, in the Hautes Pyrénées, the water is cooled from 101°·9 to 99°·8 in passing through a short and well-inclosed stone conduit from the source to the 'buvette’; and in the neighbouring spring of the Mahourat, the spout from which the water flows, though in contact with the granite rock from which it rises, and, in common parlance, the true or real source, I found to give a temperature 0°·5 lower than I obtained a few feet further back by squeezing myself into an almost inaccessible cleft of the rock. Thus for the most part we have no assurance that two travellers have observed the same spring at the same point; and hence identity of name by no means infers comparability, even supposing the instruments perfect. The frequent alterations in the thermal establishments render a specific description of the locality still more indispensable. Where the mineral water is not applied to use, we have a new difficulty in the recognition of a spring by the mere description of locality. That hot springs should ever be so abundant as to render this possible might seem improbable; I have had occasion to suffer from it, however, in following the footsteps of the indefatigable Anglada amongst the numerous and often almost inaccessible hot-springs of the Eastern Pyrenees (near Thuez, in Roussillon).


2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Kawtar Fikri-Benbrahim ◽  
Asmae Houti ◽  
Abdelhakim El Ouali Lalami ◽  
Rachid Flouchi ◽  
Naoufal El Hachlafi ◽  
...  

Morocco has an important groundwater reserve, especially in the Atlas domain, corresponding to its largest water reservoir. This reserve comes from rainwater infiltrated into rocks and sediments to give rise to mineralized waters feeding many springs and having curative properties, which confer each spa-specific therapeutic indications, based on the medicinal properties of its waters. All over the world, mineral waters of thermal springs have interesting therapeutic uses to cure some diseases; unfortunately, such potential is underexploited in Morocco. This narrative review deals with the distribution of thermal springs in Morocco, the classification of their thermal waters, and their health effects, with the aim to enhance them. For this purpose, previous studies’ results on different aspects of thermal waters were searched in the most famous scientific databases, by using targeted specific keywords. Literature has shown that Morocco contains several thermal springs, scattered throughout the Middle Atlas, in the South, and in the Rif, which are regarded as waters of high therapeutic quality, whose thermal cures are often recommended to people suffering from rheumatism and locomotor system’s diseases, skin diseases such as eczema and psoriasis, cardiovascular diseases, overweight, or respiratory troubles. However, apart from the unique and real thermal station of Moulay Yacoub, the other thermal springs are exploited in an empirical and artisanal way, mainly by a local population. So, as hydrotherapy constitutes a good choice of natural therapy using water components as a complement or alternative to conventional treatments, a better understanding of the thermal springs’ distribution in Morocco, their thermal waters’ classification, and their potential health effects may enable their valorization through a better use of their waters.


2010 ◽  
Vol 80 (2) ◽  
pp. 131-143 ◽  
Author(s):  
Pedro Gonçalves ◽  
João R. Araújo ◽  
Fátima Martel

We studied the effect of some mineral waters and some of their constituents on the apical uptake of 14C-butyrate (14C-BT) and 3H-O-methyl-D-glucose (3H-OMG) by Caco-2 cells. Uptake of 14C-BT increased after a 20-minute exposure to 1 % (v/v) distilled water, and, compared to distilled water, it was decreased by Pedras Salgadas® 1 % (v/v) and Melgaço® 5 % (v/v), and increased by Vidago® 5 % (v/v). Moreover, it increased after a 48-hour exposure to Vidago® or Melgaço® waters (5 % (v/v)). Also, uptake of 14C-BT was reduced after a 20-minute exposure to MgCl2, MgSO4, or CaCl2. Uptake of 3H-OMG was reduced after a 20-minute exposure to Melgaço® water [1 % (v/v)], when compared to distilled water. Also, a 48-hour exposure to Pedras Salgadas® or Melgaço® water (5 % (v/v)) increased and decreased uptake, respectively. Finally, uptake of 3H-OMG decreased after a 20-minute exposure to MgSO4 or NaF. In conclusion, uptake of 14C-BT and 3H-OMG by Caco-2 cells is differently modulated by distinct mineral waters.


2017 ◽  
Vol 4 (2) ◽  
pp. 87-91
Author(s):  
Ekamaida Ekamaida

The soil fertility aspect is characterized by the good biological properties of the soil. One important element of the soil biological properties is the bacterial population present in it. This research was conducted in the laboratory of Microbiology University of Malikussaleh in the May until June 2016. This study aims to determine the number of bacterial populations in soil organic and inorganic so that can be used as an indicator to know the level of soil fertility. Data analysis was done by T-Test that is by comparing the mean of observation parameter to each soil sample. The sampling method used is a composite method, which combines 9 of soil samples taken from 9 sample points on the same plot diagonally both on organic soil and inorganic soil. The results showed the highest bacterial population was found in total organic soil cfu 180500000 and total inorganic soil cfu 62.500.000


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