Histological evaluation of alveolar ridge augmentation using injectable calcium phosphate bone cement in dogs

Background Traditionally, chronic obstruction of the frontal recess is managed by frontal sinus obliteration (FSO). This often requires the harvest of abdominal fat as a filler with all of its associated morbidity. In this study, we investigate the efficacy of calcium phosphate bone cement (Craniofacial Repair System [CRS]) as a material for FSO. Methods Eighteen New Zealand white rabbits were divided into three groups and carried out to two time periods. Six rabbits underwent FSO with fat autograft (control group) and 12 rabbits underwent FSO with CRS (study groups 1 and 2, respectively). At 52 weeks, six control and six study rabbits were killed. The remaining six rabbits were killed at 78 weeks. All specimens underwent radiological evaluation with spiral computed tomography (CT) followed by gross inspection. Histological evaluation was then performed to assess bony growth and to evaluate the interface of the sinus wall with the obliterative material. Results Sinuses obliterated with CRS showed complete obliteration radiographically. This was apparent at 52 weeks and remained static at 78 weeks. Histological analysis indicated persistent obliteration of the sinus cavity from 52 to 78 weeks and signs of osteoinduction. There were no complications observed as a result of the operative procedure or the materials used. Conclusion CRS is an alternative to fat autograft for FSO in this noninfected animal model. Further studies are needed to evaluate its long-term efficacy as well as its behavior in chronically infected sinuses.

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Advantage of Alveolar Ridge Augmentation with Bioactive/Bioresorbable Screws Made of Composites of Unsintered Hydroxyapatite and Poly-L-lactide

Materials ◽

10.3390/ma12223681 ◽

2019 ◽

Vol 12 (22) ◽

pp. 3681 ◽

Cited By ~ 3

Author(s):

Shintaro Sukegawa ◽

Hotaka Kawai ◽

Keisuke Nakano ◽

Kiyofumi Takabatake ◽

Takahiro Kanno ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Transcription Factor ◽

Leptin Receptor ◽

Histological Evaluation ◽

Bone Block ◽

Alveolar Ridge ◽

Alveolar Ridge Augmentation ◽

Ridge Augmentation

We studied human bone healing characteristics and the histological osteogenic environment by using devices made of a composite of uncalcined and unsintered hydroxyapatite (u-HA) and poly-L-lactide (PLLA). In eight cases of fixation, we used u-HA/PLLA screws for maxillary alveolar ridge augmentation, for which mandibular cortical bone block was used in preimplantation surgery. Five appropriate samples with screws were evaluated histologically and immunohistochemically for runt-related transcription factor 2 (RUNX2), transcription factor Sp7 (Osterix), and leptin receptor (LepR). In all cases, histological evaluation revealed that bone components had completely surrounded the u-HA/PLLA screws, and the bone was connected directly to the biomaterial. Inflammatory cells did not invade the space between the bone and the u-HA/PLLA screw. Immunohistochemical evaluation revealed that many cells were positive for RUNX2 or Osterix, which are markers for osteoblast and osteoprogenitor cells, in the tissues surrounding u-HA/PLLA. In addition, many bone marrow–derived mesenchymal stem cells were notably positive for both LepR and RUNX2. The u-HA/PLLA material showed excellent bioactive osteoconductivity and a highly biocompatibility with bone directly attached. In addition, our findings suggest that many bone marrow–derived mesenchymal stem cells and mature osteoblast are present in the osteogenic environment created with u-HA/PLLA screws and that this environment is suitable for osteogenesis.

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Fluorescent Labeling Analysis and Electron Probe Microanalysis for Alveolar Ridge Augmentation Using Calcium Phosphate Cement.

Dental Materials Journal ◽

10.4012/dmj.21.296 ◽

2002 ◽

Vol 21 (4) ◽

pp. 296-305 ◽

Cited By ~ 14

Author(s):

Kenji FUJIKAWA ◽

Akiyoshi SUGAWARA ◽

Kaoru KUSAMA ◽

Minoru NISHIYAMA ◽

Seidai MURAI ◽

...

Keyword(s):

Calcium Phosphate ◽

Calcium Phosphate Cement ◽

Electron Probe Microanalysis ◽

Electron Probe ◽

Fluorescent Labeling ◽

Alveolar Ridge ◽

Alveolar Ridge Augmentation ◽

Ridge Augmentation

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Biological Effects of Bioresorbable Materials in Alveolar Ridge Augmentation: Comparison of Early and Slow Resorbing Osteosynthesis Materials

Materials ◽

10.3390/ma14123286 ◽

2021 ◽

Vol 14 (12) ◽

pp. 3286

Author(s):

Hotaka Kawai ◽

Shintaro Sukegawa ◽

Keisuke Nakano ◽

Kiyofumi Takabatake ◽

Sawako Ono ◽

...

Keyword(s):

Alkaline Phosphatase ◽

Alveolar Bone ◽

Biological Effects ◽

Polyglycolic Acid ◽

Bone Alkaline Phosphatase ◽

Histological Evaluation ◽

Alveolar Ridge ◽

Bone Augmentation ◽

Alveolar Ridge Augmentation ◽

Ridge Augmentation

The purpose of this study was to investigate the bone healing properties and histological environment of a u-HA/PLLA/PGA (u-HA—uncalcined and unsintered hydroxyapatite, PLLA—Poly L-lactic acid, PGA—polyglycolic acid) composite device in humans, and to understand the histological dynamics of using this device for maxillofacial treatments. Twenty-one subjects underwent pre-implant maxillary alveolar ridge augmentation with mandibular cortical bone blocks using u-HA/PLLA or u-HA/PLLA/PGA screws for fixation. Six months later, specimens of these screws and their adjacent tissue were retrieved. A histological and immunohistochemical evaluation of these samples was performed using collagen 1a, ALP (alkaline phosphatase), and osteocalcin. We observed that alveolar bone augmentation was successful for all of the subjects. Upon histological evaluation, the u-HA/PLLA screws had merged with the bone components, and the bone was directly connected to the biomaterial. In contrast, direct bone connection was not observed for the u-HA/PLLA/PGA screw. Immunohistological findings showed that in the u-HA/PLLA group, collagen 1a was positive for fibers that penetrated vertically into the bone. Alkaline phosphatase was positive only in the u-HA/PLLA stroma, and the stroma was negative for osteocalcin. In this study, u-HA/PLLA showed a greater bioactive bone conductivity than u-HA/PLLA/PGA and a higher biocompatibility for direct bone attachment. Furthermore, u-HA/PLLA was shown to have the potential for bone formation in the stroma.

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Scanning electron microscopy of composite hydroxylapatite/plaster implants for bone reconstruction

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100143250 ◽

1986 ◽

Vol 44 ◽

pp. 326-327

Author(s):

J. S. Hanker ◽

B. L. Giammara

Keyword(s):

Tooth Extraction ◽

Alveolar Ridge ◽

Small Particles ◽

Alveolar Ridge Augmentation ◽

Sintered Ceramic ◽

Ridge Augmentation ◽

Fibrovascular Tissue ◽

Infrabony Defects ◽

Edentulous Patients ◽

Scanning Electron

Nonresorbable sintered ceramic hydroxylapatite (HA) is widely employed for filling defects in jaw bone. The small particles used for alveolar ridge augmentation in edentulous patients or for infrabony defects due to periodontal disease tend to scatter when implanted using water or saline as the vehicle. Larger blocks of this material used for filling sockets after tooth extraction don't fit well. Studies in our laboratory where we compared bovine serum albumin, collagen and plaster of Paris as binders to prevent particle scatter during implantation suggested that plaster was most useful for this purpose. In addition to preventing scatter of the particles, plaster enables the formation of implants of any size and.shape either prior to or during surgery. Studies with the PATS reaction have indicated that plaster acts as a scaffold for the incorporation of HA particles into bone in areas where the implant contacts either host bone or periosteum. The shape and integrity of the implant is maintained by the plaster component until it is replaced over a period of days by fibrovascular tissue.

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