Temperature and incubation time effects on growth and ochratoxin A production by Aspergillus sclerotioniger and Aspergillus lacticoffeatus on culture media

2011 ◽  
Vol 52 (3) ◽  
pp. 208-212 ◽  
Author(s):  
L. Alborch ◽  
M.R. Bragulat ◽  
M.L. Abarca ◽  
F.J. Cabañes
Keyword(s):  
Ochratoxin A ◽  
Incubation Time ◽  
Culture Media ◽  
Time Effects

scholarly journals Role of divalent cations, pH, cytoskeleton componentes and surface charge on the adhesion of Trichomonas vaginalis to a polystyrene substrate

1987 ◽  
Vol 82 (3) ◽  
pp. 379-384 ◽  
Author(s):  
Fernando Costa e Silva Filho ◽  
Cezar Antonio Elias ◽  
Wanderley de Souza
Keyword(s):  
Surface Charge ◽  
Incubation Time ◽  
Trichomonas Vaginalis ◽  
Cytochalasin B ◽  
Culture Media ◽  
Divalent Cations ◽  
Polystyrene Beads ◽  
Different Strains ◽  
Adhesion Index

The process of adhesion of three different strains of Trichomonas vaginalis to a polystyrene substrate was analysed. The process of adhesion was dependent on the time of incubation and the pH of the phosphate-buffered solution (PBS) in which the parasites were suspended. The highest indices of adhesion were observed after an incubation time of 60 min at pH 6.6. The adhesion index increased when the parasites were incubated in the presence of culture media or when Ca++ or Mg++ was added to the PBS solution, whereas cytochalasin B, trypsin or neuraminidase reduced adhesion. Incubation of the parasites in the presence of poly-L-lysine facilitated the process of adhesion. Incubation of the parasites or polystyrene beads in the presence of poly-L-lysine led to important changes in their surface charge.

Effect of Moisture and Incubation Time on Ochratoxin A Production by an Isolate of Aspergillus ochraceus

1970 ◽  
Vol 53 (1) ◽  
pp. 89-91
Author(s):  
A F Schindler ◽  
Stanley Nesheim
Keyword(s):  
Statistical Analysis ◽  
Moisture Content ◽  
Ochratoxin A ◽  
Incubation Time ◽  
Maximum Yield ◽  
Water Levels ◽  
Aspergillus Ochraceus ◽  
Ambient Temperatures ◽  
Incubation Periods ◽  
Transfer Needle

Abstract Experiments were conducted to determine the culture requirements that would insure a probability of high ochratoxin production with a given isolate. Experiments at ambient temperatures (72 ± 2°F) determined the levels of the two most readily controlled variables, time and moisture content, during the growth of a high toxin-producing isolate (M298) of Aspergillus ochraceus. Tests were conducted in 2.8 L - Fernbach flasks containing 100 g shredded wheat and inoculated with spores on a transfer needle. Amounts of ochratoxin A/flask were determined by comparison against standards on TLC after chloroform extraction. Statistical analysis showed a relation between ochratoxin yield, water levels, and incubation periods. A water level of 40–70 ml/flask with an incubation period of 19-21 days at ambient temperatures should give a near to maximum yield of ochratoxin A.

ATP-Content and Kinetics of Acrosome Reaction in Human Spermatozoa: Influence of Various Culture Media and Incubation Time

Andrologia ◽  
2009 ◽  
Vol 20 (2) ◽  
pp. 169-172 ◽  
Author(s):  
S. DOLCI ◽  
C. MANNA ◽  
E.A. JANNINI ◽  
E. PICCIONE ◽  
N. PASETTO
Keyword(s):  
Incubation Time ◽  
Acrosome Reaction ◽  
Culture Media ◽  
Human Spermatozoa ◽  
Atp Content ◽  
Kinetics Of

Effects of temperature and incubation time on production of ochratoxin A by black aspergilli

2004 ◽  
Vol 155 (10) ◽  
pp. 861-866 ◽  
Author(s):  
Alexandre Esteban ◽  
M. Lourdes Abarca ◽  
M. Rosa Bragulat ◽  
F. Javier Cabañes
Keyword(s):  
Ochratoxin A ◽  
Incubation Time ◽  
Black Aspergilli ◽  
Effects Of Temperature

Microbially corrected amino acid composition of rumen-undegraded feed protein and amino acid degradability in the rumen of feeds enclosed in nylon bags

1986 ◽  
Vol 56 (1) ◽  
pp. 131-140 ◽  
Author(s):  
T. Varvikko
Keyword(s):  
Amino Acid ◽  
Incubation Time ◽  
Average Rate ◽  
Barley Straw ◽  
Dilution Method ◽  
Time Effects ◽  
Rumen Degradation ◽  
The Difference ◽  
Feed Protein ◽  
N Dilution Method

1. In the previous work (Varvikko & Lindberg, 1985), 15N-labelled rapeseed (Brassica napus), barley, ryegrass (Lolium perenne) and barley straw were incubated in the rumen in nylon bags for 5, 12 and 24 h and microbial nitrogen in the residues was quantified using the feed 15N-dilution method. In the present study, residual amino acids (AA) of these feeds were analysed, and microbially corrected AA of feed origin (feed AA) were estimated as the difference between total residual AA and respective microbial AA, assuming a constant AA composition for the microbial protein.2. In barley and barley-straw residues, and also in ryegrass incubated in the rumen for 24 h, very large enrich- ment by microbial N and AA-N was found. The microbial enrichment was rather small in rapeseed residues and ryegrass incubated for 5 or 12 h. During the rumen incubation, feed N and AA-N (g/kg feed dry matter (DM)) decreased very clearly in all the feeds, and feed and incubation time effects were always statistically significant (P < 0.001).3. The slow degradation of essential (E) feed AA compared with the respective non-essential (NE) AA degradation increased the proportion of feed EAA (g/kg determined feed AA) in barley and barley-straw residues. In rapeseed and ryegrass, residual feed EAA: NEAA remained very similar to the original. Branched-chain (Br) AA tended to increase proportionally in all the feed residues, suggesting these AA to be, on average, more resistant against microbial degradation in the rumen than other AA. Similarly, lysine was clearly increased in barley residues. A rumen degradation faster than the average rate caused decreased residual feed glutamic acid in rapeseed; methionine, alanine and glycine in barley; arginine and alanine in ryegrass; and methionine, asparagine and tyrosine in barley straw. Feed and incubation time effects were significant (P < 0.054–001) for feed AA (g/kg determined feed AA) grouped as EAA, BrAA or NEAA, and for most individual AA, as well as for feed AA disappearance (%) and relative amounts (%) of feed AA in the respective residual AA.4. According to present findings, AA composition of the rumen-undegraded vegetable feed residues may markedly differ, either quantitatively or qualitatively (or both), from their original AA composition. When determining the feed AA composition of nylon-bag residues, the microbial error may be very large with starchy or fibrous feeds of low protein content. The microbial AA do not, however, considerably confuse the AA determination of protein-rich feeds.

scholarly journals Activity of acetic acid and sodium bicarbonate to inhibit Asergillus ochraceus on culture media and detoxification of ochratoxin A in poultry diet

2020 ◽  
Author(s):  
Halima Z. Hussein ◽  
Mneer Saed Al-Baldawy ◽  
Rakib. A. Hamed
Keyword(s):  
Acetic Acid ◽  
Sodium Bicarbonate ◽  
Ochratoxin A ◽  
Culture Media ◽  
Aspergillus Ochraceus ◽  
Toxin A ◽  
Similar Reduction

AbstractThe study was conducted to evaluate the activity of acetic acid and sodium bicarbonate to inhibit Aspergillus ochraceus on culture media and reduce ochra toxin A in poultry diet. It was found that addition of acetic acid into PDA medium 0.2%, 0.5% and 4% could reduce A ochraceus growth by 90.22%, 98.77and 100%, respectively. Similar reduction in A ochraceus growth was obtained on PDA containing sodium bicarbonate that attained to 81%, 91.5%, and 100% at 4%, 2% and 5% concentrations, respectively. Results showed that addition of acetic acid at 0.5% and sodium bicarbonate at 1% could reduce ochre A mycotoxin by 65.7% and 43.6%, respectively in the first month and then reached to 100% in the second month for the two compounds.

scholarly journals Valorization of Date Syrup by the Production of Lipopeptide Biosurfactants by a Bacillus mojavensis BI2 strain: Bioprocess Optimization by Response Surface Methodology and Study of Surface Activities

2021 ◽  
Author(s):  
Mnif Inès ◽  
Amir Bouallegue ◽  
Salwa Mekki ◽  
Dhouha Ghribi
Keyword(s):  
Response Surface ◽  
Incubation Time ◽  
Culture Media ◽  
Biosurfactant Production ◽  
Scale Production ◽  
Surface Activities ◽  
Date Syrup ◽  
Lipopeptide Biosurfactants ◽  
Great Surface

Abstract Lipopeptides Biosurfactants are natural surface-active compounds produced by a variety of microorganisms. They have great interest in environmental, biomedical and agro-industrial fields. However, the high cost of culture media and the low yield of production limit their large-scale production and application. The development of efficient and cost-effective bioprocess became of a great interest for the improvement of the yield of biosurfactants and the decrease of production cost. In this aim, we applied the response surface method to optimize an economic biosurfactant production by a newly isolated strain B. mojavensis BI2 on date syrup called “Luegmi” as unique carbon and nitrogen source. Using a Box-Bhenken design, we studied the effect of three independent variables on lipopeptide production; Leugmi concentration, Na 2 HPO 4 and incubation time. The results of this study showed that Leugmi concentration at 25%, Na 2 HPO 4 at 0.1% and incubation time of 24 hours were optimal conditions for biosurfactant production, with a maximum surface tension decreasing capacity of 55% corresponding to 27 mN/m and oil dispersing of 30 cm 2 corresponding to a diameter of 6 cm. Preliminary characterization of the biosurfactant produced on Luegmi by UV-Spectra and Thin Layer Chromatography showed its lipopeptide characters. Physic-chemical characterization of the produced lipopepetide on Leugmi showed its great surface activities and stabilities at different pH, temperature and salts concentration. The results of this study suggested that Leugmi, an agricultural byproducts can be used as a low-cost substrate to enhance the yield of lipopeptide biosurfactants with great surface activities for potential environmental application.

Influence of pH and Incubation Time on Ochratoxin A Production by Aspergillus carbonarius in Culture Media

2005 ◽  
Vol 68 (7) ◽  
pp. 1435-1440 ◽  
Author(s):  
A. ESTEBAN ◽  
M. L. ABARCA ◽  
M. R. BRAGULAT ◽  
F. J. CABAÑES
Keyword(s):  
Ochratoxin A ◽  
Culture Media ◽  
Aspergillus Carbonarius ◽  
Yeast Autolysate ◽  
Initial Ph ◽  
Wide Ph Range ◽  
Ph Range ◽  
Incubation Temperatures ◽  
Influence Of Ph ◽  
Czapek Yeast Autolysate Agar

The effect of pH (2 to 10) and temperature (15 and 30°C) on growth and production of ochratoxin A (OTA) of six strains of Aspergillus carbonarius was studied in two culture media: Czapek yeast autolysate agar and yeast extract sucrose agar. Isolates were selected by their different source and different reported ability to produce OTA. Regardless of the initial pH or the temperature tested, Czapek yeast autolysate agar has been shown to be the best culture medium for OTA production by A. carbonarius. In this medium, OTA was produced from pH 2 to 10 at the two incubation temperatures tested. The results obtained show the ability of A. carbonarius to not only grow but also produce OTA over a wide pH range at high or low temperatures. This may help explain why this species is considered the main OTA source in some substrata.

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