Real-time contrast-enhanced ultrasound determination of microvascular blood volume in abdominal subcutaneous adipose tissue in man. Evidence for adipose tissue capillary recruitment

2010 ◽  
Vol 30 (6) ◽  
pp. 447-452 ◽  
Author(s):  
L. Tobin ◽  
L. Simonsen ◽  
J. Bülow
Keyword(s):  
Adipose Tissue ◽  
Real Time ◽  
Blood Volume ◽  
Subcutaneous Adipose Tissue ◽  
Contrast Enhanced Ultrasound ◽  
Capillary Recruitment ◽  
Contrast Enhanced ◽  
Subcutaneous Adipose

A new method to study changes in microvascular blood volume in muscle and adipose tissue: real-time imaging in humans and rat

2011 ◽  
Vol 301 (2) ◽  
pp. H450-H458 ◽  
Author(s):  
Kim A. Sjøberg ◽  
Stephen Rattigan ◽  
Natalie Hiscock ◽  
Erik A. Richter ◽  
Bente Kiens
Keyword(s):  
Adipose Tissue ◽  
Steady State ◽  
Real Time ◽  
Blood Volume ◽  
Subcutaneous Adipose Tissue ◽  
Contrast Enhanced Ultrasound ◽  
Perfusion Rate ◽  
Real Time Imaging ◽  
Contrast Enhanced ◽  
Subcutaneous Adipose

We employed and evaluated a new application of contrast-enhanced ultrasound for real-time imaging of changes in microvascular blood volume (MBV) in tissues in females, males, and rat. Continuous real-time imaging was performed using contrast-enhanced ultrasound to quantify infused gas-filled microbubbles in the microcirculation. It was necessary to infuse microbubbles for a minimum of 5–7 min to obtain steady-state bubble concentration, a prerequisite for making comparisons between different physiological states. Insulin clamped at a submaximal concentration (∼75 μU/ml) increased MBV by 27 and 39% in females and males, respectively, and by 30% in female subcutaneous adipose tissue. There was no difference in the ability of insulin to increase muscle MBV in females and males, and microvascular perfusion rate was not increased significantly by insulin. However, perfusion rate of the microvascular space was higher in females compared with males. In rats, insulin clamped at a maximal concentration increased muscle MBV by 60%. Large increases in microvascular volume and perfusion rate were detected during electrical stimulation of muscle in rats and immediately after exercise in humans. We have demonstrated that real-time imaging of changes in MBV is possible in human and rat muscle and in subcutaneous adipose tissue and that the method is sensitive enough to pick up relatively small changes in MBV when performed with due consideration of steady-state microbubble concentration. Because of real-time imaging, the method has wide applications for determining MBV in different organs during various physiological or pathophysiological conditions.

scholarly journals GIP-induced vasodilation in human adipose tissue involves capillary recruitment

2019 ◽  
Vol 8 (6) ◽  
pp. 806-813 ◽  
Author(s):  
Meena Asmar ◽  
Ali Asmar ◽  
Lene Simonsen ◽  
Flemming Dela ◽  
Jens Juul Holst ◽  
...  
Keyword(s):  
Blood Flow ◽  
Adipose Tissue ◽  
Blood Volume ◽  
Tissue Blood Flow ◽  
Contrast Enhanced Ultrasound ◽  
Lipid Uptake ◽  
Abdominal Adipose Tissue ◽  
Capillary Recruitment ◽  
Contrast Enhanced ◽  
Subcutaneous Abdominal Adipose Tissue

Glucose-dependent insulinotropic polypeptide (GIP) in combination with hyperinsulinemia increase blood flow and triglyceride clearance in subcutaneous abdominal adipose tissue in lean humans. The present experiments were performed to determine whether the increase involves capillary recruitment. Eight lean healthy volunteers were studied before and after 1 h infusion of GIP or saline during a hyperglycemic–hyperinsulinemic clamp, raising plasma glucose and insulin to postprandial levels. Subcutaneous abdominal adipose tissue blood flow (ATBF) was measured by the 133Xenon clearance technique, and microvascular blood volume was determined by contrast-enhanced ultrasound imaging. During infusion of saline and the clamp, both ATBF (2.7 ± 0.5 mL/min 100 g/tissue) and microvascular blood volume remained unchanged throughout the experiments. During GIP infusion and the clamp, ATBF increased ~fourfold to 11.4 ± 1.9 mL/min 100 g/tissue, P < 0.001. Likewise, the contrast-enhanced ultrasound signal intensity, a measure of the microvascular blood volume, increased significantly 1 h after infusion of GIP and the clamp (P = 0.003), but not in the control experiments. In conclusion, the increase in ATBF during GIP infusion involves recruitment of capillaries in healthy lean subjects, which probably increases the interaction of circulating lipoproteins with lipoprotein lipase, thus promoting adipose tissue lipid uptake.

GC determination of nicotine in subcutaneous adipose tissue obtained by minimal trauma tissue biopsy

2000 ◽  
Vol 51 (1) ◽  
pp. S237-S240 ◽  
Author(s):  
B. X. Mayer ◽  
M. Brunner ◽  
M. Müller ◽  
H. Mascher ◽  
H. G. Eichler
Keyword(s):  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Tissue Biopsy ◽  
Minimal Trauma ◽  
Subcutaneous Adipose

scholarly journals Subcutaneous, Paracardiac, and Epicardial Fat CT Density Before/After Contrast Injection: Any Correlation with CAD?

2021 ◽  
Vol 10 (4) ◽  
pp. 735
Author(s):  
Caterina Beatrice Monti ◽  
Davide Capra ◽  
Alexis Malavazos ◽  
Giorgia Florini ◽  
Carlo Parietti ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Epicardial Adipose Tissue ◽  
Tissue Density ◽  
Contrast Enhanced ◽  
Potential Biomarker ◽  
Artery Disease ◽  
Ct Density ◽  
Enhanced Computed Tomography ◽  
Subcutaneous Adipose

Adipose tissue, in particular epicardial adipose tissue, has been identified as a potential biomarker of cardiovascular pathologies such as coronary artery disease (CAD) in the light of its metabolic activity and close anatomic and pathophysiologic relationship to the heart. Our purpose was to evaluate epicardial adipose tissue density at both unenhanced and contrast-enhanced computed tomography (CT), along with CT densities of paracardiac and subcutaneous adipose tissue, as well as the relations of such densities with CAD. We retrospectively reviewed patients who underwent cardiac CT at our institution for CAD assessment. We segmented regions of interest on epicardial, paracardiac, and subcutaneous adipose tissue on unenhanced and contrast-enhanced scans. A total of 480 patients were included, 164 of them presenting with CAD. Median epicardial adipose tissue density measured on contrast-enhanced scans (−81.5 HU; interquartile range −84.9 to −78.0) was higher than that measured on unenhanced scans (−73.4 HU; −76.9 to −69.4) (p < 0.001), whereas paracardiac and subcutaneous adipose tissue densities were not (p ≥ 0.055). Patients with or without CAD, did not show significant differences in density of epicardial, paracardiac, and subcutaneous adipose tissue either on unenhanced or contrast-enhanced scans (p ≥ 0.092). CAD patients may experience different phenomena (inflammation, fibrosis, increase in adipose depots) leading to rises or drops in epicardial adipose tissue density, resulting in variations that are difficult to detect.

scholarly journals Comparative study of visceral adiposity index and lipid profile with Body Mass Index in adolescent and young adults

2019 ◽  
Vol 6 (6) ◽  
pp. 1722
Author(s):  
T. Anusha ◽  
Y. Sree Harsha ◽  
D. S. S. K. Raju
Keyword(s):  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Visceral Adiposity ◽  
Visceral Adiposity Index ◽  
Age Group ◽  
Adult Age ◽  
Adiposity Index ◽  
Waist Hip Ratio ◽  
Subcutaneous Adipose

Background: Obesity is associated with increased rate of mortality and morbidity if untreated. Obesity is a health hazard it required attention towards adolescent and adult age group. Generally, body fat is distributed into two main compartments that are subcutaneous adipose tissue and visceral adipose tissue. Waist Hip Ratio measures the subcutaneous adipose tissue, determination of visceral adiposity will further helpful for determination of cardiovascular disease.Methods: The study comprised of 120 subjects, of these adolescents comprised of 60 healthy adolescent childrens. The adults comprised of 60 healthy individuals. In all the subjects, Height was estimated in centimetres and Weight was measured in kilogram measured and BMI was calculated. The waist hip ratio and visceral adiposity index was calculated. In all the subjects, venous blood was collected from all the subjects for lipid profile estimation.Results: In present study BMI more than 25 have soon increased waist hip ratio and visceral adiposity index value than BMI less than 25 and it’s statically significant in both adolescent and adult group. It is also reported that BMI more than 25 have increased level of mean serum triglycerides, serum total cholesterol, LDL cholesterol and VLDL the increase was statistically significant. The mean serum HDL cholesterol was significantly decreased in BMI>25 group when compared with BMI<25.Conclusions: It was concluded that different age groups of adolescent and adult, appropriate methods should be planned specifically for adolescent and adult age group. Regular educational sessions are needed to make changes adolescent and adults toward obesity.

Sign in / Sign up

Export Citation Format

Share Document