Quantitative analysis of hTERT mRNA expression in colorectal cancer

2001 ◽  
Vol 96 (6) ◽  
pp. 1895-1900 ◽  
Author(s):  
Hideaki Niiyama ◽  
Kazuhiro Mizumoto ◽  
Norihiro Sato ◽  
Eishi Nagai ◽  
Ryuichi Mibu ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Quantitative Analysis ◽  
Mrna Expression ◽  
Htert Mrna ◽  
Htert Mrna Expression

Prognostic relevance of hTERT mRNA expression in ductal adenocarcinoma of the pancreas

2008 ◽  
Vol 46 (09) ◽  
Author(s):  
LF Grochola ◽  
T Greither ◽  
P Möller ◽  
D Henne-Bruns ◽  
P Würl
Keyword(s):  
Mrna Expression ◽  
Ductal Adenocarcinoma ◽  
Htert Mrna ◽  
Prognostic Relevance ◽  
Adenocarcinoma Of The Pancreas ◽  
Htert Mrna Expression

scholarly journals EGFR and hTERT Expression as a Diagnostic Approach for Non-small Cell Lung Cancer in High Risk Groups

2010 ◽  
Vol 2 ◽  
pp. BIC.S3383 ◽  
Author(s):  
Radostina Cherneva ◽  
Ognian Georgiev ◽  
Ivanka Dimova ◽  
Blaga Rukova ◽  
Danail Petrov ◽  
...  
Keyword(s):  
Gene Expression ◽  
Lung Cancer ◽  
High Risk ◽  
Small Cell Lung Cancer ◽  
Mrna Expression ◽  
Small Cell ◽  
Htert Mrna ◽  
Small Cell Lung ◽  
The Mean ◽  
Htert Mrna Expression

Objective The early detection of NSCLC is of importance because it provides chances for better outcomes. The aim of the study was to explore the clinical utility of EGFR and hTERT mRNA expression as markers for diagnosis of NSCLC. Methods EGFR and hTERT mRNA were quantified by quantative reverse transcription real time polymerase chain reaction in plasma of 45 non-small cell lung cancer (NSCLC) and 40 chronic obstructive pulmonary disease (COPD) patients, selected by certain spirometric characteristics that made them at high risk of developing lung cancer in future. Results The gene expression level of each gene was calculated and given as a relative quantity–-RQ. EGFR gene expression was found in all lung cancer patients. The mean level of expression was RQ = 29.39. hTERT mRNA could be detected in 88% of patients. The mean expression ratio in them was RQ = 17.31. Only 50% of the high risk patients turned to be positive for EGFR. The level of their expression was RQ = 2.09. The plasma levels of hTERT could be detected in 17 (42.5%) patients of the high risk COPD group. Their mean level of expression was RQ = 1.02. A statistically significant difference in EGFR and hTERT mRNA expression could be observed between the two groups of patients–-p = 0.0001. Conclusion EGFR and hTERT mRNA are potential markers for lung cancer diagnosis, whose clinical importance should be replicated in a larger cohort of patients.

scholarly journals miR-19b regulates hTERT mRNA expression through targeting PITX1 mRNA in melanoma cells

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Takahito Ohira ◽  
Sunamura Naohiro ◽  
Yuji Nakayama ◽  
Mitsuhiko Osaki ◽  
Futoshi Okada ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Melanoma Cells ◽  
Htert Mrna ◽  
Htert Mrna Expression

The Regulation of Telomere Binding Proteins on Telomerase during Differentiation of ATRA Responsive and Resistant NB4 Cells.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 3390-3390
Author(s):  
He Huang ◽  
Jie Sun ◽  
Yuan Yuan Zhu ◽  
Jian Ping Lan ◽  
Xiao Yu Lai
Keyword(s):  
Protein Expression ◽  
Mrna Expression ◽  
Peak Level ◽  
Telomerase Activity ◽  
Expression Level ◽  
Htert Mrna ◽  
Down Regulation ◽  
Nb4 Cells ◽  
Significant Change ◽  
Htert Mrna Expression

Abstract It has been reported that the down-regulation of telomerase activity associated with maturation of APL cells is not mechanistically linked to cell maturation, and requires only a RAR, but not a RXR-dependent pathway. However, it is not clear whether and how telomeric proteins respond to the retinoid treatment. Using maturation-sensitive and resistant APL cell lines NB4, NB4-R1 and NB4-R2 cells, we analyzed a panel of telomeric proteins using western blotting analyses in addition to temporal profile of corresponding mRNA during the course of retinoid-induced differentiation. Our analyses show hTERTmRNA expression decreased rapidly during differentiation of NB4 and NB4-R1 cells, telomerase activity also declined. But in NB4-R2 cells, hTERT mRNA was initially decreased to 38.2% on day1 (P<0.05) and then increased to 80.0% on day 3 (P<0.05). Telomerase activity remained unchanged overtime (P>0.05), which may be caused by the increasing of hTERT mRNA expression during its later period of differentiation. TRF1 mRNA and protein expression have no significant change during differentiation in NB4 and NB4-R1 cells but has a small increase in NB4-R2 cells. The TRF1 mRNA expression level has no significant change during differentiation of NB4 and NB4-R1 cell line cells. However, it was increased to 235% on day 2 (P<0.05) and remains at this level until day 3 during the differentiation of NB4-R2 cells. TRF1 protein expression level also remains stable during differentiation of NB4 and NB4-R1 cells, but has a little increase in NB4-R2 cells. This indicates TRF1 has different regulation in RARα dependent or RXRα dependent pathways. Pinx1 mRNA expression decreased during the differentiation of NB4 and NB4-R1. But during the differentiation of NB4-R2, Pinx1mRNA expressions level was initially decreased to 34.3% (P<0.05) on day 1 then increased to 64.5% (P<0.05 compared to day1) on day2. The change of Pinx1 mRNA expression and hTERT mRNA expression in NB4(r=0.902, P=0.036), NB4-R1(r=1.00, P<0.001), and NB4-R2(r=0.880, P=0.049) cells are positive correlated. Pinx1 is the only telomere binding protein that can bind to hTERT directly, it might be responsible for the different regulation of telomerase activity through RARα dependent or RXRα dependent pathways. During NB4 cell differentiation, TANK1 mRNA expression decreased gradually to 31.6% (P<0.05) on day1, and remained this level until day3. In NB4-R1 cells, TANK1 mRNA expression increased initially to 197% at 12h(P<0.05), and then decreased gradually to 111% (P<0.05) on day 3. During the differentiation of NB4-R2 cells, TANK1 mRNA expression was initially increased to 204% at 12h(P<0.01), and then decreased gradually to 96.9% on day3 (P<0.01). Its protein expression initially increased and reached a peak level at day 1 and then decreased in the later period of differentiation of all three NB4 cells. Both TANK1 mRNA expression and its protein expression were down-regulated at the later period of differentiation in all three NB4 cells. It seems that TANK1 may act as a positive regulator on telomerase activity during differentiation. TANK2 mRNA expression remained no change during differentiation of three NB4 cells. As results show, Pinx1 and TANK1 may interfere in the regulation of telomerase. The decrease of TANK1 may be the cause of the down-regulation of telomerase activity. Further studies will focus on the mechanism of their regulation on telomerase.

The evaluation of hTERT mRNA expression in acute leukemia children and 2 years follow-up of 40 cases

2008 ◽  
Vol 87 (3) ◽  
pp. 276-283 ◽  
Author(s):  
Ozgur Cogulu ◽  
Buket Kosova ◽  
Cumhur Gunduz ◽  
Emin Karaca ◽  
Serap Aksoylar ◽  
...  
Keyword(s):  
Acute Leukemia ◽  
Mrna Expression ◽  
Htert Mrna ◽  
Htert Mrna Expression

Effect of indomethacin on the growth response, c-myc mRNA expression, and hTERT mRNA expression in colon cancer cells

2001 ◽  
Vol 120 (5) ◽  
pp. A165-A165
Author(s):  
T SHIMADA ◽  
T KUNIYOSHI ◽  
K KOJIMA ◽  
Y MITOBE ◽  
T MITSUHASHI ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Mrna Expression ◽  
Cancer Cells ◽  
Growth Response ◽  
Htert Mrna ◽  
Colon Cancer Cells ◽  
Htert Mrna Expression
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