scholarly journals Agrobacterium strains isolated from root nodules of common bean specifically reduce nodulation by Rhizobium gallicum

2006 ◽  
Vol 56 (2) ◽  
pp. 304-309 ◽  
Author(s):  
Moncef Mrabet ◽  
Bacem Mnasri ◽  
Samir Ben Romdhane ◽  
Gisèle Laguerre ◽  
Mohamed Elarbi Aouani ◽  
...  
Keyword(s):  
Common Bean ◽  
Root Nodules ◽  
Rhizobium Gallicum

Differential colonization by bioprospected rhizobial bacteria associated with common bean in different cropping systems

2017 ◽  
Vol 63 (8) ◽  
pp. 682-689
Author(s):  
Josiele Polzin de Oliveira-Francesquini ◽  
Mariangela Hungria ◽  
Daiani Cristina Savi ◽  
Chirlei Glienke ◽  
Rodrigo Aluizio ◽  
...  
Keyword(s):  
Common Bean ◽  
Plant Biomass ◽  
Cropping Systems ◽  
Root Nodules ◽  
Housekeeping Genes ◽  
Rrna Gene ◽  
N Fixation ◽  
N Accumulation ◽  
Symbiotic Efficiency ◽  
Plant Nitrogen

In this study, we evaluated the diversity of rhizobia isolated from root nodules on common bean (Phaseolus vulgaris) derived from Andean and Mesoamerican centers and grown under field and greenhouse conditions. Genetic characterization of isolates was performed by sequencing analyses of the 16S rRNA gene and 2 housekeeping genes, recA and glnII, and by the amplification of nifH. Symbiotic efficiency was evaluated by examining nodulation, plant biomass production, and plant nitrogen (N) accumulation. The influence of the environment was observed in nodulation capacity, where Rhizobium miluonense was dominant under greenhouse conditions and the Rhizobium acidisoli group prevailed under field conditions. However, strain LGMB41 fit into a separate group from the type strain of R. acidisoli in terms of multilocus phylogeny, implying that it could belong to a new species. Rhizobium miluonense LGMB73 showed the best symbiotic efficiency performance, i.e., with the highest shoot-N content (77.7 mg/plant), superior to the commercial standard strain (56.9 mg/plant). Biodiversity- and bioprospecting-associated studies are important to better understand ecosystems and to develop more effective strategies to improve plant growth using a N-fixation process.

Novel Rhizobium lineages isolated from root nodules of the common bean (Phaseolus vulgaris L.) in Andean and Mesoamerican areas

2013 ◽  
Vol 164 (7) ◽  
pp. 740-748 ◽  
Author(s):  
Renan Augusto Ribeiro ◽  
Ernesto Ormeño-Orrillo ◽  
Rebeca Fuzinatto Dall'Agnol ◽  
Peter H. Graham ◽  
Esperanza Martinez-Romero ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Root Nodules ◽  
Phaseolus Vulgaris L ◽  
The Common

scholarly journals Rhizobium etli and Rhizobium gallicum Nodulate Common Bean (Phaseolus vulgaris) in a Traditionally Managed Milpa Plot in Mexico: Population Genetics and Biogeographic Implications

2003 ◽  
Vol 69 (2) ◽  
pp. 884-893 ◽  
Author(s):  
Claudia Silva ◽  
Pablo Vinuesa ◽  
Luis E. Eguiarte ◽  
Esperanza Martínez-Romero ◽  
Valeria Souza
Keyword(s):  
Genetic Structure ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Host Range ◽  
Polymorphism Analysis ◽  
Enzyme Electrophoresis ◽  
Rhizobium Etli ◽  
Genetic Barrier ◽  
Temporal Isolation ◽  
Rhizobium Gallicum

ABSTRACT The stability of the genetic structure of rhizobial populations nodulating Phaseolus vulgaris cultivated in a traditionally managed milpa plot in Mexico was studied over three consecutive years. The set of molecular markers analyzed (including partial rrs, glnII, nifH, and nodB sequences), along with host range experiments, placed the isolates examined in Rhizobium etli bv. phaseoli and Rhizobium gallicum bv. gallicum. Cluster analysis of multilocus enzyme electrophoresis and plasmid profile data separated the two species and identified numerically dominant clones within each of them. Population genetic analyses showed that there was high genetic differentiation between the two species and that there was low intrapopulation differentiation of the species over the 3 years. The results of linkage disequilibrium analyses are consistent with an epidemic genetic structure for both species, with frequent genetic exchange taking place within conspecific populations but not between the R. etli and R. gallicum populations. A subsample of isolates was selected and used for 16S ribosomal DNA PCR-restriction fragment length polymorphism analysis, nifH copy number determination, and host range experiments. Plasmid profiles and nifH hybridization patterns also revealed the occurrence of lateral plasmid transfer among distinct multilocus genotypes within species but not between species. Both species were recovered from nodules of the same plants, indicating that mechanisms other than host, spatial, or temporal isolation may account for the genetic barrier between the species. The biogeographic implications of finding an R. gallicum bv. gallicum population nodulating common bean in America are discussed.

scholarly journals Rhizobium paranaense sp. nov., an effective N2-fixing symbiont of common bean (Phaseolus vulgaris L.) with broad geographical distribution in Brazil

2014 ◽  
Vol 64 (Pt_9) ◽  
pp. 3222-3229 ◽  
Author(s):  
Rebeca Fuzinatto Dall’Agnol ◽  
Renan Augusto Ribeiro ◽  
Jakeline Renata Marçon Delamuta ◽  
Ernesto Ormeño-Orrillo ◽  
Marco Antonio Rogel ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Novel Species ◽  
Sequence Similarity ◽  
Root Nodules ◽  
Biochemical Properties ◽  
Rhizobium Tropici ◽  
Content Type ◽  
Link Type ◽  
Whole Genomes

Nitrogen (N), the nutrient most required for plant growth, is key for good yield of agriculturally important crops. Common bean (Phaseolus vulgaris L.) can benefit from bacteria collectively called rhizobia, which are capable of fixing atmospheric nitrogen (N2) in root nodules and supplying it to the plant. Common bean is amongst the most promiscuous legume hosts; several described species, in addition to putative novel ones have been reported as able to nodulate this legume, although not always effectively in terms of fixing N2. In this study, we present data indicating that Brazilian strains PRF 35T, PRF 54, CPAO 1135 and H 52, currently classified as Rhizobium tropici , represent a novel species symbiont of common bean. Morphological, physiological and biochemical properties differentiate these strains from other species of the genus Rhizobium , as do BOX-PCR profiles (less than 60 % similarity), multilocus sequence analysis with recA, gyrB and rpoA (less than 96.4 % sequence similarity), DNA–DNA hybridization (less than 50 % DNA–DNA relatedness), and average nucleotide identity of whole genomes (less than 92.8.%). The novel species is effective in nodulating and fixing N2 with P. vulgaris, Leucaena leucocephala and Leucaena esculenta. We propose the name Rhizobium paranaense sp. nov. for this novel taxon, with strain PRF 35T ( = CNPSo 120T = LMG 27577T = IPR-Pv 1249T) as the type strain.

scholarly journals Symbiotic Efficiency of Native Rhizobia Nodulating Common Bean (Phaseolus vulgaris L.) in Soils of Western Kenya

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Fanuel Kawaka ◽  
Mathews M. Dida ◽  
Peter A. Opala ◽  
Omwoyo Ombori ◽  
John Maingi ◽  
...  
Keyword(s):  
Common Bean ◽  
Reference Strain ◽  
Root Nodules ◽  
Dry Weight ◽  
Symbiotic Efficiency ◽  
Shoot Dry Weight ◽  
Yeast Extract Mannitol ◽  
History Of ◽  
Red Bean ◽  
Pure Bacterial Cultures

This study was conducted to determine the abundance and symbiotic efficiency of native rhizobia nodulating common bean in Kisumu and Kakamega, Kenya. Soil sampling was carried out in three farms that had been used for growing common bean for at least two seasons and one fallow land with no known history of growing common bean or inoculation. Abundance of soil rhizobia and symbiotic efficiency (SE) were determined in a greenhouse experiment. Native rhizobia populations ranged from 3.2×101 to 3.5×104 cells per gram of soil. Pure bacterial cultures isolated from fresh and healthy root nodules exhibited typical characteristics of Rhizobium sp. on yeast extract mannitol agar media supplemented with Congo red. Bean inoculation with the isolates significantly p<0.05 increased the shoot dry weight and nitrogen (N) concentration and content. The SE of all the native rhizobia were higher when compared to a reference strain, CIAT 899 (67%), and ranged from 74% to 170%. Four isolates had SE above a second reference strain, Strain 446 (110%). Our results demonstrate the presence of native rhizobia that are potentially superior to the commercial inoculants. These can be exploited to enhance bean inoculation programmes in the region.

Characterization of root-associated bacterial species of Leklek variety of common bean (Phaseolus sp.)

2021 ◽  
Vol 26 (5) ◽  
pp. 3008-3013
Author(s):  
DİLEK TEKDAL ◽  
◽  
İLKNUR AKÇA ◽  
ASLI KÜÇÜKRECEP ◽  
SELİM ÇETİNER ◽  
...  
Keyword(s):  
Common Bean ◽  
Ribosomal Rna ◽  
Root Nodules ◽  
Bacterial Species ◽  
Its Region ◽  
23S Rrna ◽  
Nodule Formation ◽  
Rrna Gene ◽  
Microbacterium Paraoxydans ◽  
23S Ribosomal Rna

The common bean is a valuable food source in the human diet. Leklek is a local variety of common bean (Phaseolus sp.) widely grown in Mersin's Gülnar district, but little is known about this variety. In the present study, bacterial species from root nodules of this common bean variety were identified by PCR-amplified 16S ribosomal RNA (rRNA) gene and 16S-23S ribosomal RNA (rRNA) Internal Transcribed Spacer (ITS) region and sequencing. The partial 16S rRNA gene and 16S-23S rRNA ITS region sequences were submitted to the NCBI database (accession numbers MT967369, MT968518, respectively). Amplified sequences were used to construct a phylogenetic tree. Phylogenetic analysis based on the identified sequences showed that the isolate belonged to the genus Microbacterium and was closely related to Microbacterium paraoxydans. The findings presented here will provide a clue for understanding this bacterium's role in nodule formation in Phaseolus sp. (variety Leklek).

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