Pseudomonas corrugata contains a conserved N-acyl homoserine lactone quorum sensing system; its role in tomato pathogenicity and tobacco hypersensitivity response

ABSTRACT Burkholderia mallei has two acyl-homoserine lactone (acyl-HSL) signal generator-receptor pairs and two additional signal receptors, all of which contribute to virulence. We show that B. mallei produces N-3-hydroxy-octanoyl HSL (3OHC8-HSL) but a bmaI3 mutant does not. Recombinant Escherichia coli expressing BmaI3 produces hydroxylated acyl-HSLs, with 3OHC8-HSL being the most abundant compound. In recombinant E. coli, BmaR3 responds to 3OHC8-HSL but not to other acyl-HSLs. These data indicate that the signal for BmaR3-BmaI3 quorum sensing is 3OHC8-HSL.

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Characterization of a luxI/luxR-type quorum sensing system and N-acyl-homoserine lactone-dependent regulation of exo-enzyme and antibacterial component production in Serratia plymuthica RVH1

Research in Microbiology ◽

10.1016/j.resmic.2006.11.008 ◽

2007 ◽

Vol 158 (2) ◽

pp. 150-158 ◽

Cited By ~ 40

Author(s):

Rob Van Houdt ◽

Pieter Moons ◽

Abram Aertsen ◽

An Jansen ◽

Kristof Vanoirbeek ◽

...

Keyword(s):

Quorum Sensing ◽

Homoserine Lactone ◽

Acyl Homoserine Lactone ◽

Serratia Plymuthica ◽

Sensing System ◽

Quorum Sensing System ◽

Component Production

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Insights into the Genome Sequences of an N -Acyl Homoserine Lactone Molecule Producing Two Pseudomonas spp. Isolated from the Arctic

Genome Announcements ◽

10.1128/genomea.00767-16 ◽

2016 ◽

Vol 4 (4) ◽

Cited By ~ 3

Author(s):

Akhilandeswarre Dharmaprakash ◽

Dinesh Reghunathan ◽

Krishnakutty C. Sivakumar ◽

Manoj Prasannakumar ◽

Sabu Thomas

Keyword(s):

Quorum Sensing ◽

Draft Genome ◽

Homoserine Lactone ◽

The Arctic ◽

Acyl Homoserine Lactone ◽

Cold Environments ◽

Sensing System ◽

Quorum Sensing System ◽

First Time

We report for the first time the draft genome sequence of two psychrotrophic Pseudomonas species, Pseudomonas simiae RGCB 73 and Pseudomonas brenneri RGCB 108, from the Arctic that produce more than one acyl homoserine lactone molecule of varied N -acyl length. The study confirms the presence of a LuxR-LuxI (type) mediated quorum-sensing system in both the Pseudomonas species and enables us to understand the role of quorum sensing in their survival in extremely cold environments.

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Inhibition of exoprotease production in Aeromonas hydrophila by rhizome extract of temu lawak (Curcuma xanthorrhiza (Roxb.)

Biofarmasi Journal of Natural Product Biochemistry ◽

10.13057/biofar/f040202 ◽

2006 ◽

Vol 4 (2) ◽

pp. 45-54

Author(s):

UMI LESTARI ◽

ARTINI PANGASTUTI ◽

ARI SUSILOWATI

Keyword(s):

Quorum Sensing ◽

Ethyl Acetate ◽

Aeromonas Hydrophila ◽

Ethanol Extract ◽

Homoserine Lactone ◽

Sensing System ◽

Development Of Resistance ◽

Quorum Sensing System ◽

Assay Result ◽

Curcuma Xanthorrhiza

Conventional treatment of infectious diseases is based on compounds that kill or inhibit the growth of bacteria. A major concern with this approach is the frequent development of resistance to antimicrobial compounds. The discovery of communication (quorum sensing system) regulating bacterial virulence opens up ways to control certain bacterial infectious without interfering the growth. The fish pathogen Aeromonas hydrophila produces quorum sensing signal, NButanoyl-L-Homoserine Lactone (C4-HSL). C4-HSL regulates exoprotease synthesis, a virulence factor of A. hydrophila. Expression of exoprotease can be blocked by using quorum sensing inhibitor. The purpose of this study was to investigate the inhibiting effect of Curcuma xanthorrhiza (Roxb.) extract to exoprotease production of A. hydrophila. Extraction was conducted by using n-hexane, ethyl acetate and ethanol. The qualitative exoprotease assay result showed that n-hexane extract of C. xanthorrhiza had not effect on growth and exoprotease production of A. hydrophila. Meanwhile, 4% of ethyl acetate and ethanol extract of C. xanthorrhiza can inhibit exoprotease production without affecting A. hydrophilla growth. The quantitative exoprotease assay result showed that 4% of ethyl acetate and ethanol extract can inhibit the exoprotease production by 93,9% and 95,6%. The growth of A. hydrophila was not affected by this extract.

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PqsE Functions Independently of PqsR-Pseudomonas Quinolone Signal and Enhances the rhl Quorum-Sensing System

Journal of Bacteriology ◽

10.1128/jb.00753-08 ◽

2008 ◽

Vol 190 (21) ◽

pp. 7043-7051 ◽

Cited By ~ 105

Author(s):

John M. Farrow ◽

Zoe M. Sund ◽

Matthew L. Ellison ◽

Dana S. Wade ◽

James P. Coleman ◽

...

Keyword(s):

Quorum Sensing ◽

Virulence Factors ◽

Opportunistic Pathogen ◽

Homoserine Lactone ◽

Chronic Infections ◽

Signaling Systems ◽

Sensing System ◽

Pseudomonas Quinolone Signal ◽

Quorum Sensing System ◽

Hostile Environments

ABSTRACT Pseudomonas aeruginosa is an opportunistic pathogen that causes both acute and chronic infections in immunocompromised individuals. This gram-negative bacterium produces a battery of virulence factors that allow it to infect and survive in many different hostile environments. The control of many of these virulence factors falls under the influence of one of three P. aeruginosa cell-to-cell signaling systems. The focus of this study, the quinolone signaling system, functions through the Pseudomonas quinolone signal (PQS), previously identified as 2-heptyl-3-hydroxy-4-quinolone. This signal binds to and activates the LysR-type transcriptional regulator PqsR (also known as MvfR), which in turn induces the expression of the pqsABCDE operon. The first four genes of this operon are required for PQS synthesis, but the fifth gene, pqsE, is not. The function of the pqsE gene is not known, but it is required for the production of multiple PQS-controlled virulence factors and for virulence in multiple models of infection. In this report, we show that PqsE can activate PQS-controlled genes in the absence of PqsR and PQS. Our data also suggest that the regulatory activity of PqsE requires RhlR and indicate that a pqsE mutant can be complemented for pyocyanin production by a large excess of exogenous N-butyryl homoserine lactone (C4-HSL). Finally, we show that PqsE enhances the ability of Escherichia coli expressing RhlR to respond to C4-HSL. Overall, our data lead us to conclude that PqsE functions as a regulator that is independent of PqsR and PQS but dependent on the rhl quorum-sensing system.

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The LuxM Homologue VanM from Vibrio anguillarumDirects the Synthesis of N-(3-Hydroxyhexanoyl)homoserine Lactone and N-Hexanoylhomoserine Lactone

Journal of Bacteriology ◽

10.1128/jb.183.12.3537-3547.2001 ◽

2001 ◽

Vol 183 (12) ◽

pp. 3537-3547 ◽

Cited By ~ 87

Author(s):

Debra L. Milton ◽

Victoria J. Chalker ◽

David Kirke ◽

Andrea Hardman ◽

Miguel Cámara ◽

...

Keyword(s):

Quorum Sensing ◽

Vibrio Anguillarum ◽

Homoserine Lactone ◽

Regulatory Elements ◽

Sequencing Error ◽

Infection Model ◽

Reading Frame ◽

Acylhomoserine Lactone ◽

Sensing System ◽

Quorum Sensing System

ABSTRACT Vibrio anguillarum, which causes terminal hemorrhagic septicemia in fish, was previously shown to possess a LuxRI-type quorum-sensing system (vanRI) and to produceN-(3-oxodecanoyl)homoserine lactone (3-oxo-C10-HSL). However, a vanI null mutant still activatedN-acylhomoserine lactone (AHL) biosensors, indicating the presence of an additional quorum-sensing circuit in V. anguillarum. In this study, we have characterized this second system. Using high-pressure liquid chromatography in conjunction with mass spectrometry and chemical analysis, we identified two additional AHLs as N-hexanoylhomoserine lactone (C6-HSL) andN-(3-hydroxyhexanoyl)homoserine lactone (3-hydroxy-C6-HSL). Quantification of each AHL present in stationary-phase V. anguillarum spent culture supernatants indicated that 3-oxo-C10-HSL, 3-hydroxy-C6-HSL, and C6-HSL are present at approximately 8.5, 9.5, and 0.3 nM, respectively. Furthermore,vanM, the gene responsible for the synthesis of these AHLs, was characterized and shown to be homologous to the luxLand luxM genes, which are required for the production ofN-(3-hydroxybutanoyl)homoserine lactone in Vibrio harveyi. However, resequencing of the V. harveyi luxL/luxM junction revealed a sequencing error present in the published sequence, which when corrected resulted in a single open reading frame (termed luxM). Downstream ofvanM, we identified a homologue of luxN(vanN) that encodes a hybrid sensor kinase which forms part of a phosphorelay cascade involved in the regulation of bioluminescence in V. harveyi. A mutation in vanM abolished the production of C6-HSL and 3-hydroxy-C6-HSL. In addition, production of 3-oxo-C10-HSL was abolished in the vanM mutant, suggesting that 3-hydroxy-C6-HSL and C6-HSL regulate the production of 3-oxo-C10-HSL via vanRI. However, a vanN mutant displayed a wild-type AHL profile. Neither mutation affected either the production of proteases or virulence in a fish infection model. These data indicate that V. anguillarum possesses a hierarchical quorum sensing system consisting of regulatory elements homologous to those found in both V. fischeri (the LuxRI homologues VanRI) and V. harveyi (the LuxMN homologues, VanMN).

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The Plant Pathogen Pantoea ananatis Produces N-Acylhomoserine Lactone and Causes Center Rot Disease of Onion by Quorum Sensing

Journal of Bacteriology ◽

10.1128/jb.01054-07 ◽

2007 ◽

Vol 189 (22) ◽

pp. 8333-8338 ◽

Cited By ~ 53

Author(s):

Tomohiro Morohoshi ◽

Yuta Nakamura ◽

Go Yamazaki ◽

Akio Ishida ◽

Norihiro Kato ◽

...

Keyword(s):

Quorum Sensing ◽

Biofilm Formation ◽

Receptor Gene ◽

Homoserine Lactone ◽

Pantoea Ananatis ◽

Signal Molecule ◽

Acylhomoserine Lactone ◽

Sensing System ◽

Quorum Sensing System ◽

Rot Disease

ABSTRACT A number of gram-negative bacteria have a quorum-sensing system and produce N-acyl-l-homoserine lactone (AHL) that they use them as a quorum-sensing signal molecule. Pantoea ananatis is reported as a common colonist of wheat heads at ripening and causes center rot of onion. In this study, we demonstrated that P. ananatis SK-1 produced two AHLs, N-hexanoyl-l-homoserine lactone (C6-HSL) and N-(3-oxohexanoyl)-l-homoserine lactone (3-oxo-C6-HSL). We cloned the AHL-synthase gene (eanI) and AHL-receptor gene (eanR) and revealed that the deduced amino acid sequence of EanI/EanR showed high identity to those of EsaI/EsaR from P. stewartii. EanR repressed the ean box sequence and the addition of AHLs resulted in derepression of ean box. Inactivation of the chromosomal eanI gene in SK-1 caused disruption of exopolysaccharide (EPS) biosynthesis, biofilm formation, and infection of onion leaves, which were recovered by adding exogenous 3-oxo-C6-HSL. These results demonstrated that the quorum-sensing system involved the biosynthesis of EPS, biofilm formation, and infection of onion leaves in P. ananatis SK-1.

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