scholarly journals Human colostrum contains IgA antibodies reactive to colonization factors I and II of enterotoxigenicEscherichia coli

2006 ◽  
Vol 47 (2) ◽  
pp. 199-206 ◽  
Author(s):  
Simone Corrêa ◽  
Patricia Palmeira ◽  
Magda Maria Sales Carneiro-Sampaio ◽  
Lucília Sanae Nishimura ◽  
Beatriz Ernestina Cabilio Guth
Keyword(s):  
Human Colostrum ◽  
Iga Antibodies ◽  
Colonization Factors

Human IgA inhibits adherence ofAcanthamoeba polyphagato epithelial cells and contact lenses

2004 ◽  
Vol 50 (9) ◽  
pp. 711-718 ◽  
Author(s):  
Rafael Campos-Rodríguez ◽  
Gabriela Oliver-Aguillón ◽  
Luz M Vega-Pérez ◽  
Adriana Jarillo-Luna ◽  
Dolores Hernández-Martínez ◽  
...  
Keyword(s):  
Contact Lenses ◽  
Mdck Cells ◽  
Secretory Iga ◽  
Acanthamoeba Polyphaga ◽  
Madin Darby Canine Kidney ◽  
Corneal Epithelial ◽  
Human Colostrum ◽  
Iga Antibodies ◽  
Darby Canine Kidney

Specific anti-Acanthamoeba IgA antibodies have been detected in the serum and tears of patients and healthy individuals. However, the role of human secretory IgA antibodies in inhibiting the adherence of Acanthamoeba had not been previously investigated. Therefore, the purpose of this study was to purify secretory IgA from human colostrum and analyze its effect on the adherence of Acanthamoeba trophozoites to contact lenses and Madin–Darby canine kidney (MDCK) cells. IgA antibodies to Acanthamoeba polyphaga in colostrum of healthy women as well as in saliva and serum of healthy subjects were analyzed by ELISA and Western blot analysis. In serum, saliva, and colostrum, we detected IgA antibodies that recognized several antigens of A. polyphaga. In addition, colostrum and IgA antibodies purified from it inhibited adherence of A. polyphaga trophozoites to contact lenses and MDCK cells. These results suggest that IgA antibodies may participate in the resistance to the amoebic infection, probably by inhibiting the adherence of the trophozoites to contact lenses and corneal epithelial cells.Key words: Acanthamoeba polyphaga, free-living amoebas, colostrum, IgA.

Human Colostrum Contains IgA Antibodies Reactive to EnteropathogenicEscherichia coli Virulence-Associated Proteins: Intimin, BfpA, EspA, and EspB

1998 ◽  
Vol 27 (2) ◽  
pp. 166-171 ◽  
Author(s):  
Ivana Loureiro ◽  
Gad Frankel ◽  
Jeannette Adu-Bobie ◽  
Gordon Dougan ◽  
Luiz R. Trabulsi ◽  
...  
Keyword(s):  
Human Colostrum ◽  
Iga Antibodies ◽  
Associated Proteins

scholarly journals Homologous and Cross-Reactive Immune Responses to Enterotoxigenic Escherichia coli Colonization Factors in Bangladeshi Children

2006 ◽  
Vol 74 (8) ◽  
pp. 4512-4518 ◽  
Author(s):  
Firdausi Qadri ◽  
Firoz Ahmed ◽  
Tanvir Ahmed ◽  
Ann-Mari Svennerholm
Keyword(s):  
Mononuclear Cells ◽  
Geometric Mean ◽  
Immunoglobulin A ◽  
Antibody Responses ◽  
Enterotoxigenic Escherichia Coli ◽  
Healthy Children ◽  
Peripheral Blood Mononuclear ◽  
Antibody Secreting Cell ◽  
Iga Antibodies ◽  
Colonization Factors

ABSTRACT We have studied homologous (HoM) and cross-reacting (CR) immunoglobulin A (IgA) antibody responses to colonization factors (CFs) in Bangladeshi children with diarrhea due to enterotoxigenic E. coli (ETEC) strains of the CF antigen I (CFA/I) group (CFA/I, n = 25; coli surface antigen 4 [CS4], n = 8; CS14, n = 11) and the CS5 group (CS5, n = 15; CS7, n = 8), respectively. The responses to the HoM, CR, and heterologous (HeT) CF antigens in each group of patient were studied and compared to that seen in healthy children (n = 20). In the CFA/I group (CFA/I and CS14), patients responded with antibody-secreting cell (ASC) responses to HoM CFs (geometric mean, 156 to 329 ASCs/106 peripheral blood mononuclear cells [PBMCs]) and to CR CFs (≈15 to 38 ASCs/106 PBMCs) but least of all to the HeT CS5 antigen (2 to 4 ASCs/106 PBMCs). For the CS5 group of patients with ETEC (CS5 and CS7), likewise, responses to HoM CFs (230 to 372 ASCs/106 PBMCs) and CR CFs (27 to 676 ASCs/106 PBMCs) were seen, along with lower responses to the HeT CFA/I antigen (9 to 38 ASCs/106 PBMCs). Both groups of patients responded with CF-specific IgA antibodies to HoM and CR antigens in plasma but responded less to the HeT CFs. The responses in patients were seen very soon after the onset of diarrhea and peaked around 1 week after onset. Vaccinees who had received two doses of the oral, killed whole-cell ETEC vaccine (CF-BS-ETEC) responded with plasma IgA antibodies to CFA/I, a component of the vaccine, but also to the CR CS14 antigen, which was not included in the vaccine, showing that antibody responses can be stimulated by a CFA/I-containing ETEC vaccine to a CR-reacting antigen in individuals in countries where ETEC is endemic.

scholarly journals Recognition of Enteropathogenic Escherichia coli Virulence Determinants by Human Colostrum and Serum Antibodies

2000 ◽  
Vol 38 (7) ◽  
pp. 2696-2700 ◽  
Author(s):  
Aurora Parissi-Crivelli ◽  
Joaquín M. Parissi-Crivelli ◽  
Jorge A. Girón
Keyword(s):  
Escherichia Coli ◽  
Immunoglobulin A ◽  
Virulence Gene ◽  
Gene Products ◽  
Virulence Determinants ◽  
Enteropathogenic Escherichia Coli ◽  
E Coli ◽  
Human Colostrum ◽  
The World ◽  
Iga Antibodies

Human colostra and sera collected from Mexican mothers and their children at birth and 6 months thereafter were studied for the presence of antibodies against the bundle-forming pilus and several chromosomal virulence gene products (intimin and secreted proteins EspA and EspB) of enteropathogenic Escherichia coli (EPEC). Among 21 colostrum samples studied, 76, 71.5, 57, and 47% of them contained immunoglobulin A (IgA) antibodies against EspA, intimin, EspB, and BfpA, respectively. Interestingly, there was a difference in IgG response to EPEC antigens between the sera from neonates and sera from the same children 6 months later. While the number of neonates reacting to Esps and intimin diminished when they reached 6 months of age, those reacting with BfpA increased from 9 to 71%. Intimin from an enterohemorrhagic E. coli strain was also recognized by most of the samples reacting with EPEC intimin. These data suggest that Bfp and Esps elicit an antibody response during the early days of life of neonates and support the value of breast-feeding in areas of the world where bacterial diarrheal infections are endemic.

Human colostrum: a natural source of probiotics?

2001 ◽  
Vol 77 (4) ◽  
pp. 265-70 ◽  
Author(s):  
Franz R. Novak ◽  
João Aprígio Guerra de Almeida ◽  
Graciete O. Vieira ◽  
Luciana M. Borba
Keyword(s):  
Natural Source ◽  
Human Colostrum

IgA Antibodies in the Vaginal Secretion after Vaccination with SolcoTrichovac

1983 ◽  
Vol 23 (2) ◽  
pp. 46-49 ◽  
Author(s):  
R. Milovanović ◽  
R. Grčić ◽  
L. Stojković
Keyword(s):  
Vaginal Secretion ◽  
Iga Antibodies

scholarly journals Antibody in Middle Ear Fluid of Children Originates Predominantly from Sera and Nasopharyngeal Secretions

2012 ◽  
Vol 19 (10) ◽  
pp. 1593-1596 ◽  
Author(s):  
Ravinder Kaur ◽  
Thomas Kim ◽  
Janet R. Casey ◽  
Michael E. Pichichero
Keyword(s):  
Middle Ear ◽  
Acute Otitis Media ◽  
Secretory Iga ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Two Dimensional Gel Electrophoresis ◽  
Middle Ear Fluid ◽  
Iga Antibodies ◽  
Iga Antibody ◽  
Entire Array

ABSTRACTThe human middle ear is devoid of any immunocompetent cells in normal mucosa. We sought to determine the source of antibody present in the middle ear of children. Total IgG, IgA, and secretory IgA antibodies were determined by enzyme-linked immunosorbent assay from the nasopharyngeal, middle ear, and serum samples of children with acute otitis media. The two-dimensional gel electrophoresis pattern of the entire array of IgA antibodies in the nasal wash (NW) and middle ear fluid (MEF) was compared from the MEF and NW samples using isoelectric focusing and Western blotting. The total IgG and IgA antibodies in the MEF and NW samples of 137 children were compared. The ratio of IgG to IgA in the MEF was significantly different (P< 0.008) compared to NW because IgA levels were higher and IgG levels lower in NW. The IgG/IgA ratio of MEF resembled serum consistent with transudation to the MEF. Small amounts of secretory IgA were detected in MEF but the electrophoresis patterns of the entire array of IgA antibodies in the MEF and NW were virtually identical in each child evaluated; thus, IgA in MEF derived predominantly from serum and the nasopharynx by reflux via the Eustachian tube. The IgG/IgA antibody levels in the MEF and the same composition of IgA antibody in the MEF and NW identifies the predominant source of antibody in the MEF as a transudate of serum combined with nasal secretions refluxed from the nasopharynx in children.

scholarly journals Rapid seroconversion and persistent functional IgG antibodies in severe COVID-19 patients correlates with an IL-12p70 and IL-33 signature

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ariel Munitz ◽  
L. Edry-Botzer ◽  
M. Itan ◽  
R. Tur-Kaspa ◽  
D. Dicker ◽  
...  
Keyword(s):  
Nucleocapsid Protein ◽  
Neutralizing Antibodies ◽  
Host Response ◽  
Humoral Response ◽  
Rapid Onset ◽  
Response Analysis ◽  
Receptor Binding Domain ◽  
Igg Antibodies ◽  
Viral Receptor ◽  
Iga Antibodies

AbstractDespite ongoing efforts to characterize the host response toward SARS-CoV-2, a major gap in our knowledge still exists regarding the magnitude and duration of the humoral response. Analysis of the antibody response in mild versus moderate/severe patients, using our new developed quantitative electrochemiluminescent assay for detecting IgM/IgA/IgG antibodies toward SARS-CoV-2 antigens, revealed a rapid onset of IgG/IgA antibodies, specifically in moderate/severe patients. IgM antibodies against the viral receptor binding domain, but not against nucleocapsid protein, were detected at early stages of the disease. Furthermore, we observed a marked reduction in IgM/IgA antibodies over-time. Adapting our assay for ACE2 binding-competition, demonstrated that the presence of potentially neutralizing antibodies is corelated with IgG/IgA. Finally, analysis of the cytokine profile in COVID-19 patients revealed unique correlation of an IL-12p70/IL33 and IgG seroconversion, which correlated with disease severity. In summary, our comprehensive analysis has major implications on the understanding and monitoring of SARS-CoV-2 infections.

scholarly journals Presence of anti-Toxoplasma antibodies in humans and their cats in the urban zone of Guadalajara

1999 ◽  
Vol 32 (5) ◽  
pp. 483-488 ◽  
Author(s):  
María de la Luz Galván Ramírez ◽  
Guillermo Sánchez Vargas ◽  
Marcos Vielma Sandoval ◽  
Juan Luis Soto Mancilla
Keyword(s):  
Risk Factors ◽  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Toxoplasma Infection ◽  
Urban Zone ◽  
Lack Of Control ◽  
Iga Antibodies

Cats are the definitive hosts of Toxoplasma gondii. Infected cats excrete oocysts in their feces, infecting humans and other animals. The objective of the present study was to determine the presence of anti-Toxoplasma antibodies in cat owners and their pets, and determine if there was a relationship between Toxoplasma infection and humans who live with infected cats. IgG anti-Toxoplasma antibodies in sera of 59 cat owners were determined by enzyme-linked immunosorbent assay (ELISA), in 24 sera from their cats, IgG, IgM, and IgA antibodies were found using Burney's ELISA. Thirty-eight (64%) of 59 cat owners were positive to IgG anti-Toxoplasma. Seropositivity for cats was 70.8% IgG, 8.3% IgM, and 62.5% IgA. Cohabitation with cats infected by T. gondii, feeding with leftovers or raw viscera, and lack of control over how their feces were handled are risk factors conducive for humans to become infected by T. gondii.

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