EVALUATION OF THE PETRIFILMTMAND REDIGELTMAS RAPID METHODS TO THE STANDARD PLATE COUNT METHOD FOR THE ENUMERATION OF PROCESSED MEATS AND ENVIRONMENTAL SAMPLES

2000 ◽  
Vol 8 (1) ◽  
pp. 65-70 ◽  
Author(s):  
JENNIFER L. SCHMELDER ◽  
ROBIN M. KALINOWSKI ◽  
PETER W. BODNARUK
Keyword(s):  
Environmental Samples ◽  
Plate Count ◽  
Rapid Methods ◽  
Processed Meats ◽  
Standard Plate Count ◽  
Count Method ◽  
Standard Plate ◽  
Plate Count Method

scholarly journals Multiregional Evaluation of the SimPlate Heterotrophic Plate Count Method Compared to the Standard Plate Count Agar Pour Plate Method in Water

2000 ◽  
Vol 66 (1) ◽  
pp. 453-454 ◽  
Author(s):  
R. Wayne Jackson ◽  
Karen Osborne ◽  
Gary Barnes ◽  
Carol Jolliff ◽  
Dianna Zamani ◽  
...  
Keyword(s):  
Regression Analysis ◽  
Water Samples ◽  
Plate Count ◽  
Heterotrophic Plate Count ◽  
Plate Method ◽  
Standard Plate Count ◽  
Plate Count Agar ◽  
Count Method ◽  
Standard Plate ◽  
Plate Count Method

ABSTRACT A new SimPlate heterotrophic plate count (HPC) method (IDEXX Laboratories, Westbrook, Maine) was compared with the pour plate method at 35°C for 48 h. Six laboratories tested a total of 632 water samples. The SimPlate HPC method was found to be equivalent to the pour plate method by regression analysis (r = 0.95;y = 0.99X + 0.06).

THE EFFECT OF FREEZING ON THE STANDARD PLATE COUNT OF MILK1

1955 ◽  
Vol 18 (12) ◽  
pp. 297-299 ◽  
Author(s):  
C. K. Johns ◽  
I. Berzins
Keyword(s):  
Plate Count ◽  
Rapid Freezing ◽  
Standard Plate Count ◽  
Count Method ◽  
Standard Plate ◽  
Plate Count Method

Milk and cream samples were subjected to (a) slow or (b) rapid freezing, and analysed after 24 and 48 hours storage by the standard plate count method. Bacterial destruction was much less than that reported for liquid egg.

A COMPARISON OF TWO AND THREE DAYS INCUBATION FOR ENUMERATING RAW-MILK BACTERIA1

1972 ◽  
Vol 35 (3) ◽  
pp. 136-140
Author(s):  
C. N. Huhtanen ◽  
A. R. Brazis ◽  
W. L. Arledge ◽  
E. W. Cook ◽  
C. B. Donnelly ◽  
...  
Keyword(s):  
Incubation Period ◽  
Raw Milk ◽  
Plate Count ◽  
Standard Plate Count ◽  
Count Method ◽  
Three Samples ◽  
Incubation Periods ◽  
Standard Plate ◽  
Plate Count Method

Eighty-three samples of raw milk were assayed by the Standard Plate Count method with incubation periods of two and three days. The three-day incubation period gave higher counts (0.05 > p > 0.01). There were no appreciable problems (such as drying-out, spreaders, molds, etc.) encountered when plates were incubated the extra day. An optional three-day incubation period for the Standard Plate Count is recommended.

Comparison between the EC plate count test at 21 °C and an accelerated plate count method for assessing the keeping quality of pasteurized milk

1992 ◽  
Vol 59 (3) ◽  
pp. 431-436 ◽  
Author(s):  
Sarah A. Langford ◽  
Rohan G. Kroll
Keyword(s):  
Plate Count ◽  
Gram Negative ◽  
Rapid Methods ◽  
Pasteurized Milk ◽  
Psychrotrophic Bacteria ◽  
Commercial Interest ◽  
Count Method ◽  
Keeping Quality ◽  
Plate Count Method

The keeping quality of properly refrigerated pasteurized milk and cream is primarily determined by post-pasteurization contamination by Gram-negative psychrotrophic bacteria (Phillips et al. 1981; Schröder et al. 1982). Reliable and rapid methods of assessing the levels of contamination by these organisms are therefore of commercial interest.

Hydrophilicity and Bactericidal Effects of TiO2 Thin Films Prepared by RF Sputtering

2004 ◽  
Vol 449-452 ◽  
pp. 1261-1264 ◽  
Author(s):  
Yo Seung Song ◽  
Seon Hwa Kim ◽  
Bae Yeon Kim ◽  
Deuk Yong Lee
Keyword(s):  
Thin Films ◽  
Rf Sputtering ◽  
Angle Measurement ◽  
Wetting Angle ◽  
Plate Count ◽  
Tio2 Thin Films ◽  
Standard Plate Count ◽  
Bactericidal Effects ◽  
Standard Plate ◽  
Plate Count Method

TiO2 thin films were prepared by RF sputtering on Si wafer to investigate hydrophilicity and bactericidal effects by using AFM, XRD, standard plate count method and wetting angle measurement. Experimentally, the survival rate of E. coli was above 74% and the wetting angle of water for the 30 min UV illuminated films was 4.2o. It was found that the bactericidal efficiency was closely related to the large specific surface area caused by the roughness, however, hydrophilicity was governed by the surface defective sites rather than the roughness.

Microtiter Technique for Enumeration of Mesophiles, Psychrotrophs, and Coliforms in Raw and Pasteurized Milk

1977 ◽  
Vol 40 (11) ◽  
pp. 795-797 ◽  
Author(s):  
IVÀN A. CASAS ◽  
NELSON LEÓN ◽  
PEDRO IZQUIERDO
Keyword(s):  
Viable Cell ◽  
Limiting Factors ◽  
Plate Count ◽  
Microbial Quality ◽  
Pasteurized Milk ◽  
Standard Plate Count ◽  
Cell Counts ◽  
Count Method ◽  
Time Space ◽  
Standard Plate

The Microtiter Count Method was compared with the Standard Plate Count (SPC) method in evaluating mesophile, psychrotroph, and coliform counts for raw and pasteurized milk samples. Statistical analysis showed that the Microtiter Count Method was reliable when compared with the SPC for making viable cell counts on these products. The Microtiter Count Method is advantageous because it saves time, space, and material; this method should be useful for developing countries where availability of testing materials, manpower, and costs are limiting factors in surveillance of microbial quality.

Isolasi dan Seleksi Bakteri Pendegradasi Solar Dari Pelabuhan Penyeberangan Kendari – Wawonii, Sulawesi Tenggara

2019 ◽  
Vol 6 (2) ◽  
pp. 995
Author(s):  
M. Rajab Sutra Mijaya ◽  
Nur Arfa Yanti ◽  
Ardiansyah Ardiansyah ◽  
Nurhayani H. Muhiddin
Keyword(s):  
Diesel Fuel ◽  
Plate Count ◽  
Diesel Fuels ◽  
Standard Plate Count ◽  
Degrading Bacteria ◽  
Standard Plate ◽  
The Media ◽  
Plate Count Method ◽  
Selection Of ◽  
Rotary Shaker

This study aimed to obtain the ability of bacteria to degrade diesel fuel. Method of this research was exploration method. Bacteria were isolated by enrichment method used SMSSe enriched diesel 2% (v/v). Selection of hidrocarbonoclastic bacteria based on the ability of to grow on agar medium solid. The bacteria in the test made in the form of suspension with Mc Farland Standard 0.5. Test of bacterial isolates were used 10% of the inoculum put in 150 mL media with different concentrations of diesel fuel were 1%, 2% and 3% and incubated on a rotary shaker at 120 rpm. Samples were taken on 1, 5, 10, 15 and 20 days to test diesel emulsion by centrifugation at a speed of 3500 rpm ±15 minutes, the comparison between the media and diesel 4:1. Growth in the amount of bacteria accounted by a Standard Plate Count method. The levels of the diesel rest calculated every sampling during incubation. The selection results of obtained diesel degrading bacteria isolates that PSI.1 PSII.1 and PSIII.2. All of bacteria have the ability to degrade diesel fuel in different treatment, the best result in lowered diesel fuel that were a concentration of 3% during 20 days of incubated at PSII.1 isolate, that have the highest ability to reduce levels of diesel up to 70,70%.Keywords: Hydrocarbonoclastic, Degradation, Diesel fuels ABSTRAK Penelitian ini bertujuan untuk mengetahui kemampuan bakteri dalam mendegradasi solar. Penelitian ini adalah penelitian eksploratif. Isolasi bakteri menggunakan metode enrichment dengan media yang digunakan yaitu SMSSe yang diperkaya solar 2% (v/v). Pemilihan bakteri hidrokarbonoklastik berdasarkan kemampuan bakteri tumbuh pada media agar padat. Inokulum bakteri dibuat dalam bentuk suspensi dengan Standar Mc Farland 0,5. Pengujian kemampuan degradasi solar dilakukan menggunakan media minimal dengan variasi konsentrasi solar 1%, 2% dan 3% dan diinkubasi pada rotary shaker. Sampel diambil pada hari ke 1, 5, 10, 15 dan 20 untuk uji emulsi solar dengan menghitung volume solar yang teremulsi. Pertumbuhan jumlah bakteri dihitung dengan metode Standard Plate Count. Kadar sisa solar dihitung setiap pengambilan sampel selama inkubasi. Hasil seleksi bakteri pendegradasi solar diperoleh tiga isolat yaitu PSI.1 PSII.1 dan PSIII.2. Ketiga isolat bakteri memiliki kemampuan mendegradasi solar pada perlakuan yang berbeda, hasil terbaik dalam menurunkan kadar solar yaitu konsentrasi 3% selama 20 hari inkubasi pada isolat PSII.1 yang memiliki kemampuan tertinggi menurunkan kadar solar hingga 70,70%. Kata kunci : Hidrokarbonoklastik, Pendegradasi, Solar

scholarly journals THE POTENCY OF LOCAL ISOLATES FROM NORTH SUMATERA IN THE DEGRADATION OF NAPHTALENE

2015 ◽  
Vol 2 (1) ◽  
pp. 466
Author(s):  
Nunuk Priyani ◽  
Erman Munir ◽  
Irmalisyah W. Panjaitan ◽  
Kabul Warsito
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Plate Count ◽  
Surface Compound ◽  
Standard Plate Count ◽  
Naphthalene Degradation ◽  
Standard Plate ◽  
Plate Count Method ◽  
Almost All ◽  
Hydrocarbon Compound

<p>The potency of biosurfactant–producing bacteria isolated from oil-contaminated coast, Belawan North Sumatera, in the degradation of hydrocarbon compound such as naphthalene has been studied. Thirteen isolates has been obtained using Bushnell Haas Agar containing 2% naphthalene as the sole carbon source. The bacterial growth was observed using Standard Plate Count method. The ability of each isolates to produce bioactive-surface compound was determined. A drop-collapsing test was performed to examine the activity of each biosurfactant. All isolates tested grew well in Bushnell Haas Broth medium. Among those, two isolates were able to remove almost all (99%) naphthalene from the medium during 15 days. Those two isolates have also shown highest concentration of biosurfactant production which is 75.180 ppm.</p><p><br /><strong>Keywords</strong>: local isolates, biosurfactant, naphthalene degradation.</p>

scholarly journals EFFECT OF PROPOLIS EXTRACT AND PROPOLIS CANDIES ON THE GROWTH OF STREPTOCOCCUS SANGUINIS ATCC 10556

2017 ◽  
Vol 10 (17) ◽  
pp. 16
Author(s):  
Rozanna Rozanna ◽  
Nurtami Soedarsono ◽  
Ratna Farida
Keyword(s):  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Brain Heart Infusion ◽  
Plate Count ◽  
Bacterial Colony ◽  
Propolis Extract ◽  
Standard Plate Count ◽  
Standard Plate ◽  
Plate Count Method ◽  
Infusion Agar

Objectives: This study aimed to analyze the effects of propolis extract and propolis candies on Streptococcus sanguinis.Methods: Bacterial suspensions were exposed to propolis extract and propolis candies. For propolis extract, a minimum inhibitory concentration(MIC) was determined using a spectrophotometer, and a minimum bactericidal concentration (MBC) was determined using a standard plate count method. Samples were cultured in brain heart infusion agar, incubated for 24 hrs, and the bacterial colony units formed on the agar plates were manually counted.Results: Propolis extract inhibits the growth of S. sanguinis at MIC 5% and MBC 10%. The colonies of S. sanguinis decreased after exposure to propolis candies.Conclusions: Propolis extract and propolis candies are effective to inhibit the growth of S. sanguinis.

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