Effects of the TAT Peptide Orientation and Relative Location on the Protein Transduction Efficiency

2012 ◽  
Vol 79 (5) ◽  
pp. 683-690 ◽  
Author(s):  
Qingguo Guo ◽  
Guojie Zhao ◽  
Fengjin Hao ◽  
Yifu Guan
Keyword(s):  
Transduction Efficiency ◽  
Protein Transduction ◽  
Relative Location ◽  
Tat Peptide ◽  
Peptide Orientation

Intracellular Traffic and Fate of Protein Transduction Domains HIV-1 TAT Peptide and Octaarginine. Implications for Their Utilization as Drug Delivery Vectors

2006 ◽  
Vol 17 (1) ◽  
pp. 90-100 ◽  
Author(s):  
Saly Al-Taei ◽  
Neal A. Penning ◽  
Jeremy C. Simpson ◽  
Shiroh Futaki ◽  
Toshihide Takeuchi ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Protein Transduction ◽  
Tat Peptide ◽  
Intracellular Traffic ◽  
Protein Transduction Domains ◽  
Hiv 1

scholarly journals Genetically modified adenoviral vector with the protein transduction domain of Tat improves gene transfer to CAR-deficient cells

2009 ◽  
Vol 29 (2) ◽  
pp. 103-109 ◽  
Author(s):  
Shihai Liu ◽  
Qinwen Mao ◽  
Weifeng Zhang ◽  
Xiaojing Zheng ◽  
Ye Bian ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Cell Surface ◽  
Fluorescent Protein ◽  
Function Analysis ◽  
Cell Types ◽  
Transduction Efficiency ◽  
Target Cells ◽  
Protein Transduction ◽  
Protein Transduction Domain ◽  
Green Fluorescent

The transduction efficiency of Ad (adenovirus) depends, to some extent, on the expression level of CAR (coxsackievirus and Ad receptor) of a target cell. The low level of CAR on the cell surface is a potential barrier to efficient gene transfer. To overcome this problem, PTD.AdeGFP (where eGFP is enhanced green fluorescent protein) was constructed by modifying the HI loop of Ad5 (Ad type 5) fibre with the Tat (trans-activating) PTD (protein transduction domain) derived from HIV. The present study showed that PTD.AdeGFP significantly improved gene transfer to multiple cell types deficient in expression of CAR. The improvement in gene transfer was not the result of charge-directed binding between the virus and the cell surface. Although PTD.AdeGFP formed aggregates, it infected target cells in a manner different from AdeGFP aggregates precipitated by calcium phosphate. In addition, PTD.AdeGFP was able to transduce target cells in a dynamin-independent pathway. The results provide some new clues as to how PTD.AdeGFP infects target cells. This new vector would be valuable in gene-function analysis and for gene therapy in cancer.

'Click' Bioconjugation of a Well-Defined Synthetic Polymer and a Protein Transduction Domain

2007 ◽  
Vol 60 (6) ◽  
pp. 410 ◽  
Author(s):  
Jean-François Lutz ◽  
Hans G. Börner ◽  
Katja Weichenhan
Keyword(s):  
Atom Transfer Radical Polymerization ◽  
Radical Polymerization ◽  
Sodium Azide ◽  
Room Temperature ◽  
Synthetic Polymer ◽  
Protein Transduction ◽  
Protein Transduction Domain ◽  
Tat Peptide ◽  
Optimal Coupling ◽  
High Yields

The copper-catalyzed 1,3-dipolar ‘click’ cycloaddition of azides and alkynes was studied to link a model synthetic polymer to a sequence-defined protein transduction domain (PTD). The bromine chain-ends of a well-defined polystyrene (PS) sample synthesized by atom transfer radical polymerization (Mn 2200 g mol–1, Mw/Mn 1.21) were first transformed into azide functions by substitution with sodium azide, and subsequently reacted with an alkyne-functionalized PTD (i.e., the oligopeptide sequence GGYGRKKRRQRRRG, also known as the TAT peptide). The click bioconjugation proceeded successfully at room temperature, thus affording the targeted PS-b-GGYGRKKRRQRRRG bioconjugate in high yields. However, a slight molar excess of polystyrene was required for optimal coupling.

scholarly journals Enhancement of HIV-1 Tat fusion protein transduction efficiency by bog blueberry anthocyanins

BMB Reports ◽  
2010 ◽  
Vol 43 (8) ◽  
pp. 561-566 ◽  
Author(s):  
Sun-Hwa Lee ◽  
Hoon-Jae Jeong ◽  
Dae-Won Kim ◽  
Eun-Jeong Sohn ◽  
Mi-Jin Kim ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Transduction Efficiency ◽  
Protein Transduction ◽  
Tat Fusion Protein ◽  
Hiv 1

Time-lag combination therapy for cerebral ischemia using the FNK protein transduction technology and an immunosuppressant, I: In vivo study

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S57-S57
Author(s):  
Ken-ichiro Kasura ◽  
Megumi Watanabe ◽  
Kumiko Takahashi ◽  
Genki Mizukoshi ◽  
Seiji Ohkubo ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Combination Therapy ◽  
Time Lag ◽  
In Vivo Study ◽  
Protein Transduction

Crosstalk Minimization Method for Eye-tracking-based 3D Display

2020 ◽  
Author(s):  
Seok Lee ◽  
Juyong Park ◽  
Dongkyung Nam
Keyword(s):  
Image Processing ◽  
Eye Tracking ◽  
Three Dimensional ◽  
Processing Method ◽  
Eye Position ◽  
Relative Location ◽  
3D Display ◽  
Minimization Method ◽  
3D Crosstalk ◽  
Pixel Value

In this article, the authors present an image processing method to reduce three-dimensional (3D) crosstalk for eye-tracking-based 3D display. Specifically, they considered 3D pixel crosstalk and offset crosstalk and applied different approaches based on its characteristics. For 3D pixel crosstalk which depends on the viewer’s relative location, they proposed output pixel value weighting scheme based on viewer’s eye position, and for offset crosstalk they subtracted luminance of crosstalk components according to the measured display crosstalk level in advance. By simulations and experiments using the 3D display prototypes, the authors evaluated the effectiveness of proposed method.

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