Abstract
Background: This study aimed to evaluate possible cytotoxic effects to gingival epithelial cells exposed to children toothpastes containing different detergent. Methods: Tissues required fort he isolation of human gingival epithelial cells were obtained by biopsy during the extraction of the impacted third molar tooth. Toothpaste solutions of different concentrations were prepared from five different children’s toothpastes with different detergent contents. Isolated gingival epithelial cells were stimulated with experimental groups consisting of toothpaste solutions (Colgate, Sensodyne, Splat, Nenedent, Perlodent) at different concentrations and a control group consissting of complete Dulbocco’s modified eagle medium. After the experiments, cell viability was evaluated using flow cytometry. Data analysis were done using One Way ANOVA test and Tukey post-hoc test. Results: In all experimental groups, there was a decrease in live cell rates and an increase in dead cell rates due to increased concentration. The statistically highest live cell ratios were detected in Splat’s toothpaste solutions after the control group and the group with the lowest viability values was determined in Colgate group (p<0.05). Conclusions: According to the results of the study, it was observed that toothpastes containing SLS affected the viability of cells more negatively than toothpastes with other detergent contents.
Effect of high level Nd:YAG laser irradiation on human gingival epithelial cells in vitro
