Resveratrol treatment reduces expression of MCP‐1, IL‐6, IL‐8 and RANTES in endometriotic stromal cells

Abstract Background: Endometriosis is a complex disease in the field of gynecology that has certain limitations for its interim treatment. Resveratrol has been recently used for the treatment of endometriosis in experimental and clinical studies, but its molecular mechanism remaines elusive. Results: In this study, based on a case-control study, we identified that a decreased BMI and altered lipid profiles were associated with endometriosis patients. We applied resveratrol treatment on human ectopic endometrial stromal cells (HEcESCs) and a rat model of endometriosis. Lipidomics analysis showed that resveratrol altered lipid profiles in HEcESCs, with the sphingolipids Cer and SM increased significantly, while FA and most phospholipids were significantly reduced. Pathway enrichment analysis showed that several lipid-associated signaling pathways could be targeted by resveratrol. Our experiments in a rat model showed that resveratrol reduced the lesion and rectified lipid profiles in rats with endometriosis. In addition, resveratrol treatment significantly increased the expression of PPARα in lesion tissues of model rats and HEcESCs of EMs patients.Conclusion: Our data reveal that the development of EMs is closely related to lipid metabolism, and resveratrol may play a therapeutic role by targeting the lipid metabolism of ectopic endometrial stromal cells in endometrosis. Our study provides valuable insights for understanding the pathogenesis and clinical treatment of endometriosis.

Download Full-text

The Effects of Resveratrol Treatment on Bcl-2 and Bax Gene Expression in Endometriotic Compared with Non-Endometriotic Stromal Cells

Iranian Journal of Public Health ◽

10.18502/ijph.v49i8.3900 ◽

2020 ◽

Author(s):

Roya KOLAHDOUZ-MOHAMMADI ◽

Ali-Akbar DELBANDI ◽

Sepideh KHODAVERDI ◽

Soheila AREFI ◽

Tahereh ARABLOU ◽

...

Keyword(s):

Gene Expression ◽

Stromal Cells ◽

Enzymatic Digestion ◽

The Other ◽

Expression Ratio ◽

Endometrial Stromal Cells ◽

Resveratrol Treatment ◽

Gene Expression Ratio ◽

Bax Gene ◽

Significant Differential Effect

Background: We aimed to examine resveratrol effects on gene expression of Bcl-2, Bax and Bcl-2/Bax ratio in endometrial stromal cells derived from women with and without endometriosis. Methods: Endometrial tissues were obtained from 40 endometriotic patients and 15 non-endometriotic controls undergoing laparoscopic surgery or hysterectomy in the gynecology ward of Rassoul Akram Hospital, Tehran, Iran from 2015 to 2017. After the enzymatic digestion, eutopic (EuESCs) and ectopic (EESCs) endometrial stromal cells from patients with endometriosis as well as endometrial stromal cells from non-endometriotic controls (CESCs) were treated with or without resveratrol (100 µM) and the levels of Bcl-2, Bax and Bcl-2/Bax gene expression ratio in the cells from all origins were examined at 6, 24 and 48 h post-treatment by real-time PCR. Results: Resveratrol treatment increased Bcl-2 expression in CESCs at 24 and 48 h and in EuESCs at 48 h (P<0.05), but had no significant effects on the expression of this gene in EESCs. On the other hand, resveratrol treatment increased Bax expression in EuESCs at 6 h and decreased its expression in EESCs at 48 h (P<0.05). Regarding the Bcl-2/Bax gene expression ratio, resveratrol treatment increased Bcl-2/Bax gene expression ratio in CESCs and EuESCs at 48 h (P<0.01). However, this treatment had no significant differential effect on Bcl-2 and Bcl-2/Bax gene expression ratio between CESCs and EuESCs at 48 h. Conclusion: Resveratrol treatment significantly increased Bcl-2/Bax gene expression ratio in EuESCs and CESCs but had no significant effect in EESCs.

Download Full-text