PR563: Comparison of new bone formation between rhBMP-2 hydroxyapatite and bovine bone in socket preservation

Abstract Background The aim of this study was to compare new bone formation, resorbed bone matrix, and fibrous enclosed residual bone substitute material in laterally augmented alveolar bone defects using allogeneic, pre-treated and cleaned human bone blocks (tested in dogs, therefore considered to be xenogeneic), and pre-treated and cleaned bovine cancellous bone blocks, both with and without a collagen membrane in order to evaluate their augmentative potential. Methods Thirty-two critical size horizontal defects were prepared in the mandible of 4 adult foxhound dogs (8 per dog, 4 on each side). After 3 months of healing, the defects were laterally augmented in a split-mouth-design with either human (HXB) or bovine solvent-preserved bone blocks (BXB). Afterwards, defects were randomly covered with a bovine collagenous membrane (HXB + M, BXB + M). After a healing interval of 6 months, percentages of new bone formation, resorbed bone matrix, and fibrous enclosed residual bone substitute material were compared. Results Results showed little new bone formation of up to 3.7 % in human bone blocks (HXB 3.7 % ± 10.2, HXB + M 0.3 %± 0.4, BXB, 0.1 % ± 0.8, BXB + M 2.6 % ± 3.2, p = > 0.05). Percentages of fibrous encapsulation were higher in human bone blocks than in bovine bone blocks (HXB 71.2 % ± 8.6, HXB + M 73.71 % ± 10.6, BXB, 60.5 % ± 27.4, BXB + M 52.5 % ± 28.4, p = > 0.05). Resorption rates differed from 44.8 % in bovine bone blocks covered with a membrane to 17.4 % in human bone blocks (HXB 17.4 % ± 7.4, HXB + M 25.9 % ± 10.7, BXB, 38.4 % ± 27.2, BXB + M 44.8 % ± 29.6, p = > 0.05). The use of additional membranes did not significantly affect results. Conclusions Within its limitations, results of this study suggest that solvent-preserved xenogenic human and bovine bone blocks are not suitable for lateral bone augmentation in dogs. Furthermore, defect coverage with a membrane does not positively affect the outcome.

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Ability of Deproteinized Cancellous Bovine Bone to Induce New Bone Formation

Journal of Periodontology ◽

10.1902/jop.2000.71.8.1258 ◽

2000 ◽

Vol 71 (8) ◽

pp. 1258-1269 ◽

Cited By ~ 113

Author(s):

Z. Schwartz ◽

T. Weesner ◽

S. Van Dijk ◽

D.L. Cochran ◽

J.T. Mellonig ◽

...

Keyword(s):

Bone Formation ◽

Bovine Bone ◽

New Bone Formation

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A Novel HA/β-TCP-Collagen Composite Enhanced New Bone Formation for Dental Extraction Socket Preservation in Beagle Dogs

Materials ◽

10.3390/ma9030191 ◽

2016 ◽

Vol 9 (3) ◽

pp. 191 ◽

Cited By ~ 8

Author(s):

Ko-Ning Ho ◽

Eisner Salamanca ◽

Kuo-Chi Chang ◽

Tsai-Chin Shih ◽

Yu-Chi Chang ◽

...

Keyword(s):

Bone Formation ◽

Dental Extraction ◽

New Bone Formation ◽

Beagle Dogs ◽

Extraction Socket ◽

Socket Preservation

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INFLUENCE OF ETHYLENE OXIDE STERILIZATION ON NEW BONE FORMATION INDUCED BY BOVINE BONE MORPHOGENETIC PROTEIN

Journal of Musculoskeletal Research ◽

10.1142/s021895770000032x ◽

2000 ◽

Vol 04 (04) ◽

pp. 287-295

Author(s):

Tarmo Pekkarinen ◽

T. Sam Lindholm ◽

Aulis Marttinen ◽

Oili Hietala ◽

Pekka Jalovaara

Keyword(s):

Bone Formation ◽

Ethylene Oxide ◽

Bone Morphogenetic Proteins ◽

Exposure Time ◽

Clinical Settings ◽

Bovine Bone ◽

New Bone Formation ◽

Morphogenetic Protein ◽

Oxide Concentration ◽

Different Temperatures

Bone morphogenetic proteins (BMPs) have the capacity to induce and accelerate bone regeneration. Before experimental and clinical settings, BMP must be sterilized. Ethylene oxide (EO) gas sterilizations at different temperatures are commonly used but the effects of that on the osteoinductive capacity of BMP have been the subject of controversy. Here, we investigated the effects of three different EO sterilization methods on the osteoinductivity of partially purified native bovine BMP (bBMP). Gelatin capsules containing 3 mg of bBMP were sterilized as follows: (i) manually inside a dessicator with 12% EO spray (20°C, exposure time 2 h); (ii) with an EO gas sterilizer (Steri-Vac 4XL, temperature 29°C, exposure time 4 h 10 min, ethylene oxide concentration 860 mg/l); (iii) with an EO gas sterilizer (Steri-Vac 5XL, temperature 42°C, exposure time 3 h, ethylene oxide concentration 700 mg/l). The sterilization processes were monitored with samples of Bacillus subtilis (3M, Attest 1264). Osteoinductivity of bBMP was verified by bioassay. After 21 days of implantation of bBMP into the muscle pouches of mice, the animals were killed and new bone formation was measured radiographically and histologically. The EO sterilization techniques used did not significantly decrease the osteoinductive activity of BMP. It is concluded that commercial EO gas equipment sterilization is effective for sterilized BMP and does not decrease the osteoinductive capacity of bovine BMP.

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Long-Term Tissue Reactions of Three Biomaterials in Craniofacial Surgery

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.361-363.1343 ◽

2007 ◽

Vol 361-363 ◽

pp. 1343-1346 ◽

Cited By ~ 1

Author(s):

Matti Peltola ◽

Kalle Aitasalo ◽

Teemu Tirri ◽

Jami Rekola ◽

A. Puntala

Keyword(s):

Bone Formation ◽

Close Contact ◽

Healing Process ◽

Tissue Growth ◽

Bovine Bone ◽

New Bone Formation ◽

Electron Microscopic ◽

Composition Profiles ◽

Tissue Reactions

Successful craniofacial reconstruction needs both a well-known and a reliable reconstruction material. However, there is often a lack of long-term knowledge of the tissue reactions and healing process in the human body. In this study, frontal sinus obliterations with bovine bone natural hydroxyapatite derivative (BHA), synthetic bioactive glass S53P4 (BAG) and hydroxyapatite cement (HAC) were investigated with clinical, histologic, scanning electron microscopic (SEM) and energy dispersive x-ray analysis (EDXA) 27, 12 and 3 years postoperatively. The aim was to determine the long-term clinical biocompatibility of the used materials. Histologic studies revealed bone formation with BHA particles and lamellar bone with BAG granule remnants in close contact to the new bone formation. In HAC reconstruction there was scattered fibroconnective tissue growth without new bone formation in the surface of HAC implantation. Neither foreign body reaction nor any abnormal findings were seen. SEM studies revealed a CaP layer on the surface of BAG granule remnants. In EDXA studies, composition profiles showed Ca-, P- and Si- rich layers on the BAG granule surface. No differences were found in CaO and P2O5 levels between BHA granules and HAC implantation and the surrounding bone. All investigated biomaterials were well tolerated in long-term applications.

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Comparison of Bone Substitutes in a Tibia Defect Model in Wistar-Rats

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.493-494.732 ◽

2011 ◽

Vol 493-494 ◽

pp. 732-738 ◽

Cited By ~ 3

Author(s):

Cornelia Ganz ◽

W. Xu ◽

G. Holzhüter ◽

W. Götz ◽

B. Vollmar ◽

...

Keyword(s):

Bone Formation ◽

Bone Substitutes ◽

Porous Matrix ◽

Bovine Bone ◽

New Bone Formation ◽

Defect Model ◽

Inflammatory Reactions ◽

Nanocrystalline Hydroxyapatite ◽

Defect Area ◽

Tibia Defect

Various bone graft substitutes were used in clinical practise in the treatment of bone defects after trauma or osteoporosis. Many synthetic biomaterials were developed in recent years primarily based on hydroxyapatite (HA). NanoBone® is a nanocrystalline hydroxyapatite (HA) embedded in a porous matrix of silica (SiO2). The ratio of HA:SiO2 varied between 76:24 (wt%; NanoBone®) and 61:39 (wt%; Nanobone® S). The two bone substitutes NB and NB S and a natural bovine bone substitute Bio-Oss® (BO) were evaluated by means of implantation in the tibia of the rat. The aim of this study was to analyze the remodelling process and to measure new bone formation and degradation after implantation of these biomaterials. A tibia defect model was used for all investigations with testing periods of 12, 21 and 84 days. (n=5 for each time point). The results showed, that all bone grafts were well accepted by the host tissue without inflammatory reactions. In comparison to the biomaterial BO, NanoBone® and NanoBone® S were quickly degraded, whereas autologous proteins were incorporated into nanopores. New bone formation was statistically higher in NanoBone® S compared to Bio-Oss® in defect area after 84 days implantation. The presence of osteoclasts in tissue sections were demonstrated by TRAP- and ED1-immunohistology.

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Porous Titanium Membranes Combined with Various Graft Materials Induce Exophytic Bone Formation in Rabbit Calvaria

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.309-311.427 ◽

2006 ◽

Vol 309-311 ◽

pp. 427-432 ◽

Cited By ~ 3

Author(s):

Y. Kim ◽

Y.H. Kown ◽

J.B. Park ◽

J.H. Chung ◽

H.N. Lim ◽

...

Keyword(s):

Bone Formation ◽

Bone Graft ◽

Porous Titanium ◽

Control Group ◽

Bovine Bone ◽

Graft Material ◽

New Bone Formation ◽

Freeze Dried ◽

Custom Made ◽

Human Cancellous Bone

The purpose of this study was to examine if the application of custom-made porous titanium membranes combined with bone graft materials promotes exophytic bone formation in rabbit calvaria. For this purpose, round decorticated calvaria sites were created using a round carbide bur. In the control group, rectangular parallelepiped-shaped porous titanium membranes (RPTMs) were placed on the decorticated sites and fixed with metal pins. In the experimental groups, RPTMs were filled with one of the following bone graft materials prior to fixing with metal pins: bovine bone mineral (BBM), demineralized freeze-dried human cortical bone (DFDB) or freeze-dried human cancellous bone (FDB). Animals were sacrificed at 8 and 12 weeks after surgery, and new bone formation was assessed by histomorphometric as well as statistical analysis. The results indicate that at 8 and 12 weeks, all the experimental groups demonstrated exophytic bone formation. At 12 weeks, DFDB group revealed the most new bone formation (p<0.05) and resorption of grafted materials (p<0.05). On the basis of these findings, we conclude that RPTMs may be used as an augmentation membrane for guided bone regeneration and DFDB as an effective bone-inducing graft material.

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Horizontal Augmentation of Chronic Mandibular Defects by the Guided Bone Regeneration Approach: A Randomized Study in Dogs

Materials ◽

10.3390/ma15010238 ◽

2021 ◽

Vol 15 (1) ◽

pp. 238

Author(s):

Anton Friedmann ◽

Stefan Fickl ◽

Kai R. Fischer ◽

Milad Dalloul ◽

Werner Goetz ◽

...

Keyword(s):

Bone Formation ◽

Randomized Study ◽

Bone Substitutes ◽

Blue Staining ◽

Collagen Membrane ◽

Bovine Bone ◽

New Bone Formation ◽

Native Bone ◽

Pin Fixation ◽

Group B

Various biomaterial combinations have been studied focusing on their ability to stabilize blood clots and maintain space under soft tissue to support new bone formation. A popular combination is Deproteinized Bovine Bone Mineral (DBBM) placed with a native collagen membrane (NCM) tacked to native bone. In this study, we compared the outcome of this treatment option to those achieved with three different graft/membrane combinations with respect to total newly occupied area and the mineralized compound inside. After bi-lateral extraction of two mandibular premolars in five adult beagles L-shaped alveolar defects were created. A total of 20 defects healed for 6 weeks resulting in chronic type bone defects. At baseline, four options were randomly allocated to five defects each: a. DBBM + NCM with a four-pin fixation across the ridge; b. DBBM + RCLC (ribose cross-linked collagen membrane); c. DBBM + NPPM (native porcine pericardium membrane); and d. Ca-sulfate (CS) + RCLC membrane. Membranes in b/c/d were not fixed; complete tensionless wound closure was achieved by CAF. Termination after 3 months and sampling followed, and non-decalcified processing and toluidine blue staining were applied. Microscopic images obtained at standardized magnification were histomorphometrically assessed by ImageJ software (NIH). An ANOVA post hoc test was applied; histomorphometric data are presented in this paper as medians and interquartile ranges (IRs). All sites healed uneventfully, all sites were sampled and block separation followed before Technovit embedding. Two central sections per block for each group were included. Two of five specimen were lost due to processing error and were excluded from group b. New bone area was significantly greater for option b. compared to a. (p = 0.001), c. (p = 0.002), and d. (p = 0.046). Residual non-bone graft area was significantly less for option d. compared to a. (p = 0.026) or c. (p = 0.021). We conclude that collagen membranes with a prolonged resorption/barrier profile combined with bone substitutes featuring different degradation profiles sufficiently support new bone formation. Tacking strategy/membrane fixation appears redundant when using these biomaterials.

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New bone formation comparison in sinuses grafted with anorganic bovine bone and β‐TCP

Clinical Oral Implants Research ◽

10.1111/clr.438_13509 ◽

2019 ◽

Vol 30 (S19) ◽

pp. 483-483

Author(s):

Guilherme Oliveira ◽

Tòlio Bonna Pignaton ◽

Carlos Eduardo Almeida Ferreira ◽

Luiz Cesar Peruzzo ◽

Elcio Marcantonio

Keyword(s):

Bone Formation ◽

Bovine Bone ◽

New Bone Formation

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