Dietary supplementation of jute leaf ( Corchorus olitorius ) modulates hepatic delta‐aminolevulinic acid dehydratase (δ‐ALAD) activity and oxidative status in high‐fat fed/low streptozotocin‐induced diabetic rats

2019 ◽  
Vol 43 (8) ◽  
Author(s):  
Jamiyu Ayodeji Saliu ◽  
Adedayo Oluwaseun Ademiluyi ◽  
Aline Augusti Boligon ◽  
Ganiyu Oboh ◽  
Maria Rosa Chitolina Schetinger ◽  
...  
Keyword(s):  
Aminolevulinic Acid ◽  
Dietary Supplementation ◽  
Diabetic Rats ◽  
Oxidative Status ◽  
Corchorus Olitorius ◽  
High Fat ◽  
Acid Dehydratase ◽  
Alad Activity

scholarly journals Towards Initial Indications for a Thiol-Based Redox Control of Arabidopsis 5-Aminolevulinic Acid Dehydratase

Antioxidants ◽  
2018 ◽  
Vol 7 (11) ◽  
pp. 152 ◽  
Author(s):  
Daniel Wittmann ◽  
Sigri Kløve ◽  
Peng Wang ◽  
Bernhard Grimm
Keyword(s):  
Aminolevulinic Acid ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Thioredoxin Reductase ◽  
Reducing Power ◽  
Reduced Form ◽  
Redox Control ◽  
In Planta ◽  
Acid Dehydratase ◽  
Reducing Conditions ◽  
Alad Activity

Thiol-based redox control is one of the important posttranslational mechanisms of the tetrapyrrole biosynthesis pathway. Many enzymes of the pathway have been shown to interact with thioredoxin (TRX) and Nicotinamide adenine dinucleotide phosphate (NADPH)-dependent thioredoxin reductase C (NTRC). We examined the redox-dependency of 5-aminolevulinic acid dehydratase (ALAD), which catalyzed the conjugation of two 5-aminolevulinic acid (ALA) molecules to porphobilinogen. ALAD interacted with TRX f, TRX m and NTRC in chloroplasts. Consequently, less ALAD protein accumulated in the trx f1, ntrc and trx f1/ntrc mutants compared to wild-type control resulting in decreased ALAD activity. In a polyacrylamide gel under non-reducing conditions, ALAD monomers turned out to be present in reduced and two oxidized forms. The reduced and oxidized forms of ALAD differed in their catalytic activity. The addition of TRX stimulated ALAD activity. From our results it was concluded that (i) deficiency of the reducing power mainly affected the in planta stability of ALAD; and (ii) the reduced form of ALAD displayed increased enzymatic activity.

scholarly journals Effects of resveratrol on biomarkers of oxidative stress and on the activity of delta aminolevulinic acid dehydratase in liver and kidney of streptozotocin-induced diabetic rats

Biochimie ◽  
2012 ◽  
Vol 94 (2) ◽  
pp. 374-383 ◽  
Author(s):  
Roberta Schmatz ◽  
Luciane Belmonte Perreira ◽  
Naiara Stefanello ◽  
Cinthia Mazzanti ◽  
Roselia Spanevello ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Aminolevulinic Acid ◽  
Diabetic Rats ◽  
Acid Dehydratase ◽  
Liver And Kidney ◽  
Biomarkers Of Oxidative Stress

scholarly journals 5-Aminolevulinic acid dehydratase deficiency porphyria: a twenty-year clinical and biochemical follow-up

1998 ◽  
Vol 44 (9) ◽  
pp. 1892-1896 ◽  
Author(s):  
Ulrich Gross ◽  
Shigeru Sassa ◽  
Karl Jacob ◽  
Jean-Charles Deybach ◽  
Yves Nordmann ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Reference Values ◽  
Aminolevulinic Acid ◽  
Compound Heterozygous ◽  
Acid Dehydratase ◽  
Heme Arginate ◽  
Alad Activity ◽  
Rate Limiting ◽  
Acute Crisis

Abstract 5-Aminolevulinic acid dehydratase (ALAD) activity in two patients with compound heterozygous 5-aminolevulinic acid dehydratase deficiency porphyria was studied over the last 20 years. The patients’ enzyme activity was <10% from 1977 to 1997. An acute crisis in each patient was successfully treated by infusion of glucose and heme arginate. After this therapy both urinary 5-aminolevulinic acid (ALA) and total porphyrins were diminished to 65% in patient B. In patient H, ALA was decreased to 80%, and total porphyrins were reduced to 15% after treatment with heme arginate and glucose. The patients remained free of symptoms after this therapy. Family studies of patient B showed cross-reactive immunological material (CRIM), in which the maternal mutation is CRIM(+), whereas the paternal mutation is CRIM(−). Incubation of erythrocyte lysates with ALA decreased porphyrin formation, whereas incubation with porphobilinogen produced porphyrin concentrations within reference values in both patients, confirming that ALAD activity is rate-limiting in these cells.

The threshold of the delta-aminolevulinic acid dehydratase (ALAD) activities reduced by lead (Pb) exposure could be 10 μg/dL without modification of ALAD G177C genotypes in Chinese Han Taiwanese.

2018 ◽  
Author(s):  
Chan-Ching Huang ◽  
Te-Yu Liu ◽  
Chen-Cheng Yang ◽  
Chao-Ling Wang ◽  
Su-Shin Lee ◽  
...  
Keyword(s):  
Aminolevulinic Acid ◽  
Lead Level ◽  
Blood Lead ◽  
Additive Models ◽  
P Value ◽  
Health Examination ◽  
Chinese Han ◽  
Acid Dehydratase ◽  
Alad Activity ◽  
Lead Workers

Background: Lead inhibits the enzymes in the heme biosynthesis, mainly the δ-aminolevulinic acid dehydratase (ALAD) activities. The aims of this study was to establish ALAD activity assay in Taiwan and analyzed the effects of lead exposure on hematological system and the ALAD activity with the modification of the ALAD genotypes. Methods: Among 121 lead workers and 117 non-exposed workers, the data were from health examination. ALAD activity was determined by the standardized method of the European Community. ALAD genotyping was using a method of PCR-RFLP. For finding a threshold effect, we used generalized additive models (GAM) and scatter plots with smoothing curve, in addition to multiple regression methods. Results: There were 229 ALAD1-1 homozygotes, 9 ALAD1-2 heterozygotes were identified, and none of ALAD2-2 homozygote. Lead workers had significantly lower ALAD activity than non-exposed group (41.6 ± 22.1 vs. 63.3 ± 14.0 U/L, p-value < 0.001). The results of multiple regressions showed the blood lead level (BLL) was profound factor associated with ALAD activity inversely. The possible threshold of BLL affecting ALAD activity was around 10 μg/dL. Conclusions: ALAD activity was inhibited by blood lead, which could be a threshold of 10 ug/dL, which ALAD activity may be adopted as a biomarker of health examination for lead workers.

scholarly journals Inhibition of δ-aminolevulinic acid dehydratase activity by cadmium in excised etiolated maize leaf segments during greening

2011 ◽  
Vol 57 (No. 7) ◽  
pp. 332-337 ◽  
Author(s):  
J. Sarangthem ◽  
M. Jain ◽  
R. Gadre
Keyword(s):  
Enzyme Activity ◽  
Aminolevulinic Acid ◽  
Substantial Reduction ◽  
Competitive Inhibitor ◽  
Cd Accumulation ◽  
Nitrogenous Compounds ◽  
Acid Dehydratase ◽  
Alad Activity ◽  
Maize Leaves ◽  
Dehydratase Activity

Supply of 0.1&ndash;0.5 mmol CdCl<sub>2</sub> inhibited &delta;-aminolevulinic acid dehydratase (EC 4.2.1.24, ALAD) activity and total chlorophylls in excised etiolated segments of maize leaves during greening. Due to cadmium supply &delta;-aminolevulinic acid (ALA) content was reduced significantly at 0.5 mmol Cd only. Also the Cd treatment decreased the protein content and accumulated significantly the Cd in the tissue. Significant correlation between Cd accumulation in the leaves and various parameters measured is observed, with the R-squared values being 0.727 with ALAD activity, 0.885 with ALA content, 0.902 with total chlorophylls and 1.00 with proteins. The % inhibition of ALAD activity by Cd was decreased in the presence of nitrogenous compounds, glutamine and NH<sub>4</sub>NO<sub>3</sub> and the observed inhibition was 25% and 16%, respectively. More substantial reduction in % inhibition of enzyme activity by Cd was observed during treatment with glutathione, a ubiquitous thiol and levulinic acid, a competitive inhibitor of ALAD, with the inhibition being only 2% and 4%, respectively. Supply of some essential metal ions, such as Mg, Zn, and Mn, also reduced the % inhibition of enzyme activity by Cd. Inclusion of varying concentrations of ALA during assay also affected the % inhibition of enzyme activity by Cd showing an increased inhibition from 17% to 53% with increasing ALA concentration. It is suggested that Cd inhibits ALAD activity by affecting the ALA binding to the enzyme and/or disrupting thiol interaction.

scholarly journals Use of Generalized Additive Model to Detect the Threshold of δ-Aminolevulinic Acid Dehydratase Activity Reduced by Lead Exposure

2020 ◽  
Vol 17 (16) ◽  
pp. 5712
Author(s):  
Chan-Ching Huang ◽  
Chen-Cheng Yang ◽  
Te-Yu Liu ◽  
Chia-Yen Dai ◽  
Chao-Ling Wang ◽  
...  
Keyword(s):  
Lead Exposure ◽  
Aminolevulinic Acid ◽  
Lead Level ◽  
Blood Lead ◽  
Additive Models ◽  
Acid Dehydratase ◽  
Alad Activity ◽  
Exposed Workers ◽  
Lead Workers ◽  
Dehydratase Activity

Background: Lead inhibits the enzymes in heme biosynthesis, mainly reducing δ-aminolevulinic acid dehydratase (ALAD) activity, which could be an available biomarker. The aim of this study was to detect the threshold of δ-aminolevulinic acid dehydratase activity reduced by lead exposure. Methods: We collected data on 121 lead workers and 117 non-exposed workers when annual health examinations were performed. ALAD activity was determined by the standardized method of the European Community. ALAD G177C (rs1800435) genotyping was conducted using the polymerase chain reaction and restricted fragment length polymorphism (PCR-RFLP) method. In order to find a threshold effect, we used generalized additive models (GAMs) and scatter plots with smoothing curves, in addition to multiple regression methods. Results: There were 229 ALAD1-1 homozygotes and 9 ALAD1-2 heterozygotes identified, and no ALAD2-2 homozygotes. Lead workers had significantly lower ALAD activity than non-exposed workers (41.6 ± 22.1 vs. 63.3 ± 14.0 U/L, p < 0.001). The results of multiple regressions showed that the blood lead level (BLL) was an important factor inversely associated with ALAD activity. The possible threshold of BLL affecting ALAD activity was around 5 μg/dL. Conclusions: ALAD activity was inhibited by blood lead at a possible threshold of 5 μg/dL, which suggests that ALAD activity could be used as an indicator for lead exposure regulation.

Erythrocyte δ-Aminolevulinic Acid Dehydratase (ALAD) Activity as an Indicator of Lead Exposure in Dogs and Cats: Optimal Test Conditions

1992 ◽  
Vol 2 (1) ◽  
pp. 57-68
Author(s):  
Philippe J. Berny ◽  
Louise M. Côté ◽  
William B. Buck
Keyword(s):  
Lead Exposure ◽  
Aminolevulinic Acid ◽  
Optimal Test ◽  
Test Conditions ◽  
Acid Dehydratase ◽  
Alad Activity

Effects of Maternal Flaxseed Supplementation on Female Offspring of Diabetic Rats in Serum Concentration of Glucose, Insulin, and Thyroid Hormones

2019 ◽  
Vol 89 (1-2) ◽  
pp. 45-54
Author(s):  
Akemi Suzuki ◽  
André Manoel Correia-Santos ◽  
Gabriela Câmara Vicente ◽  
Luiz Guillermo Coca Velarde ◽  
Gilson Teles Boaventura
Keyword(s):  
Thyroid Hormones ◽  
High Fat Diet ◽  
Female Offspring ◽  
Flaxseed Oil ◽  
Diabetic Rats ◽  
High Fat ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Flaxseed Flour ◽  
Maternal Consumption

Abstract. Objective: This study aimed to evaluate the effect of maternal consumption of flaxseed flour and oil on serum concentrations of glucose, insulin, and thyroid hormones of the adult female offspring of diabetic rats. Methods: Wistar rats were induced to diabetes by a high-fat diet (60%) and streptozotocin (35 mg/kg). Rats were mated and once pregnancy was confirmed, were divided into the following groups: Control Group (CG): casein-based diet; High-fat Group (HG): high-fat diet (49%); High-fat Flaxseed Group (HFG): high-fat diet supplemented with 25% flaxseed flour; High-fat Flaxseed Oil group (HOG): high-fat diet, where soya oil was replaced with flaxseed oil. After weaning, female pups (n = 6) from each group were separated, received a commercial rat diet and were sacrificed after 180 days. Serum insulin concentrations were determined by ELISA, the levels of triiodothyronine (T3), thyroxine (T4) and thyroid-stimulating hormone (TSH) were determined by chemiluminescence. Results: There was a significant reduction in body weight at weaning in HG (−31%), HFG (−33%) and HOG (44%) compared to CG (p = 0.002), which became similar by the end of 180 days. Blood glucose levels were reduced in HFG (−10%, p = 0.044) when compared to CG, and there was no significant difference between groups in relation to insulin, T3, T4, and TSH after 180 days. Conclusions: Maternal severe hyperglycemia during pregnancy and lactation resulted in a microsomal offspring. Maternal consumption of flaxseed reduces blood glucose levels in adult offspring without significant effects on insulin levels and thyroid hormones.

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