Combination of allyl isothiocyanate and cinnamaldehyde against the growth of mycotoxigenic fungi and aflatoxin production in corn

2021 ◽  
Author(s):  
Alberto Gonçalves Evangelista ◽  
Karla Carolina Paiva Bocate ◽  
Giuseppe Meca ◽  
Fernando Bittencourt Luciano
Keyword(s):  
Allyl Isothiocyanate ◽  
Aflatoxin Production ◽  
Mycotoxigenic Fungi

scholarly journals Inhibition of Growth of Seed-Borne Fungi and Aflatoxin Production on Stored Peanuts by Allyl Isothiocyanate Vapor

2018 ◽  
Vol 59 (1) ◽  
pp. 45-50
Author(s):  
Kiyoshi OKANO ◽  
Chikako NISHIOKA ◽  
Tetsuya IIDA ◽  
Yuzi OZU ◽  
Misao KANEKO ◽  
...  
Keyword(s):  
Allyl Isothiocyanate ◽  
Aflatoxin Production ◽  
Inhibition Of Growth

scholarly journals Inhibition of Aflatoxin Production and Fungal Growthon Stored Corn by Allyl Isothiocyanate Vapor

2015 ◽  
Vol 56 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Kiyoshi OKANO ◽  
Ayaka OSE ◽  
Mitsuhiro TAKAI ◽  
Misao KANEKO ◽  
Chikako NISHIOKA ◽  
...  
Keyword(s):  
Allyl Isothiocyanate ◽  
Aflatoxin Production

scholarly journals Fumigation of Brazil nuts with allyl isothiocyanate to inhibit the growth of Aspergillus parasiticus and aflatoxin production

2017 ◽  
Vol 98 (2) ◽  
pp. 792-798 ◽  
Author(s):  
Lucas F Lopes ◽  
Keliani Bordin ◽  
Gabriel HC de Lara ◽  
Federica Saladino ◽  
Juan M Quiles ◽  
...  
Keyword(s):  
Aspergillus Parasiticus ◽  
Allyl Isothiocyanate ◽  
Aflatoxin Production ◽  
Brazil Nuts

Climate change factors and Aspergillus flavus: effects on gene expression, growth and aflatoxin production

2015 ◽  
Vol 8 (2) ◽  
pp. 171-179 ◽  
Author(s):  
Á Medina ◽  
A. Rodríguez ◽  
Y. Sultan ◽  
N. Magan
Keyword(s):  
Climate Change ◽  
Gene Expression ◽  
Water Stress ◽  
Aspergillus Flavus ◽  
Climatic Factors ◽  
Aflatoxin Production ◽  
Scientific Data ◽  
Effect Of Temperature ◽  
Mycotoxigenic Fungi ◽  
The Impact

The objectives of this study were to obtain scientific data on the impact that interactions between water stress (water activity (aw); 0.97, 0.95, 0.92), temperature (34, 37 °C) and CO2 exposure (350, 650, 1000 ppm) may have on the growth, gene expression of biosynthetic genes (aflD, aflR), and phenotypic aflatoxin B1 (AFB1) production by a type strain of Aspergillus flavus on a conducive medium. The study showed that while aw affected growth there was no statistically significant effect of temperature or CO2 exposure. The effect of these interacting factors on aflD and aflR gene expression showed that at 34 °C there was maximum relative expression of aflD under the control conditions (34 °C, 350 ppm) with a decrease in expression with elevated CO2 and water stress. For aflR expression at 34 °C, there was a significant increase in expression, but only at 0.92 aw and 650 ppm CO2. However, at 37 °C, there was a significant increase in expression of both aflD and aflR at 0.95 and 0.92 aw and 650 and 1000 ppm CO2. There was an associated increase in AFB1 in these treatments. In contrast, at 34 °C there were no significant differences for interacting treatments. This is the first study to examine these three-way interacting climatic factors on growth and mycotoxin production by a strain of A. flavus. This provides data that are necessary to help predict the real impacts of climate change on mycotoxigenic fungi.

Ammonium Bicarbonate Inhibition of Mycotoxigenic Fungi and Spoilage Yeasts

1990 ◽  
Vol 53 (4) ◽  
pp. 324-328 ◽  
Author(s):  
DAVID A. DEPASQUALE ◽  
ANWAAR EL-NABARAWY ◽  
JOSEPH D. ROSEN ◽  
THOMAS J. MONTVILLE
Keyword(s):  
Sodium Chloride ◽  
Sodium Bicarbonate ◽  
Ammonium Sulfate ◽  
Debaryomyces Hansenii ◽  
Aflatoxin Production ◽  
Ammonium Bicarbonate ◽  
Potassium Bicarbonate ◽  
Aspergillus Ochraceus ◽  
Spoilage Yeasts ◽  
Mycotoxigenic Fungi

Sodium bicarbonate inhibits growth and aflatoxin production by Aspergillus parasiticus. This survey determined that other mycotoxigenic fungi were also sensitive to bicarbonates. Sodium bicarbonate, potassium bicarbonate, ammonium bicarbonate, ammonium sulfate, and sodium chloride were added to buffered or unbuffered potato-dextrose agar to determine the bicarbonate effect on growth and morphology of six mycotoxigenic fungi. Three nonmycotoxigenic fungi and four yeast species were also examined. Ammonium bicarbonate at 0.11M completely inhibited the growth of Fusarium tricinctum NRRL 13442, F. tricinctum NRRL 13426, F. graminearum NRRL 5883, F. sporotrichioides NRRL 3249, Penicillium griseofulvum NRRL 989, Aspergillus ochraceus NRRL 3174, A. flavus NRRL 1957, A. niger, and P. notatum. Sodium chloride and pH elevated through the use of ampso-NaOH, capso-NaOH, or glycine-NaOH buffer did not display an inhibitory effect on the filamentous fungi examined. Buffered ammonium sulfate treatments (pH approximately 9.0) completely inhibited all of the mycotoxigenic fungi, but at pH 5.6, ammonium sulfate treatments were not inhibitory. Sodium bicarbonate (0.11M) was effective only against P. griseofulvum, A. flavus NRRL 1957, A. niger, and P. notatum, causing viability reductions of 5.6, 3.7, 4.9, and 2.9 log cycles, respectively. Potassium bicarbonate was generally as inhibitory as the sodium salt. In contrast, elevated pH, alone, appeared to account for the >6 log reduction observed for the yeasts Lipomyces starkeyi, Geotrichum candidum, Kluyveromyces marxianus, and Debaryomyces hansenii.

Possible Synergistic Effect of Nisin and Propionic Acid on the Growth of the Mycotoxigenic Fungi Aspergillus parasiticus, Aspergillus ochraceus, and Fusarium moniliforme

1999 ◽  
Vol 62 (10) ◽  
pp. 1223-1227 ◽  
Author(s):  
NACHMAN PASTER ◽  
ZHOU LECONG ◽  
MAZAL MENASHROV ◽  
RONI SHAPIRA
Keyword(s):  
Propionic Acid ◽  
Spore Germination ◽  
Fusarium Moniliforme ◽  
Mycelial Growth ◽  
Aspergillus Parasiticus ◽  
Combined Treatment ◽  
Aflatoxin Production ◽  
Aspergillus Ochraceus ◽  
Stored Grain ◽  
Mycotoxigenic Fungi

The effects of nisin and propionic acid (PA) on aflatoxin production and on mycelial growth and spore germination of the mycotoxigenic fungi Aspergillus parasiticus, A. ochraceus, and Fusarium moniliforme were investigated. The growth of A. ochraceus was completely inhibited on media containing PA with nisin in concentrations of 0.05% PA with 1,000 ppm nisin, and 0.1% PA with 500 or 1,000 ppm nisin. The growth of both F. moniliforme and A. parasiticus was completely inhibited by PA with nisin at a concentration of 0.1% PA with 1,000 ppm nisin. Nisin alone caused a significant increase in mycelial growth when applied to A. ochraceus at 500 or 1,000 ppm and when applied to A. parasiticus at 1,000 ppm. Spore germination of A. ochraceus was completely inhibited on media containing 0.1% PA with 500 or 1,000 ppm nisin. Spores of F. moniliforme failed to germinate in 0.05% PA with 500 or 1,000 ppm nisin, whereas spores of A. parasiticus did not germinate on media containing 0.1% PA with 1,000 ppm nisin. For all three fungi tested, the inhibitory effect on mycelial growth was found to be fungistatic rather than fungicidal. The combined treatment of PA with nisin produced better fungistatic activity than treatment involving either material alone. Nisin, applied alone, did not stimulate aflatoxin production (expressed by μg toxin/mg mycelium), but the combined treatment at certain concentrations was inhibitory to aflatoxin B1 or G1. The production of aflatoxin G1, but not of B1, was stimulated in 0.05% PA with 1,000 ppm nisin and on media containing 0.1% PA with 100 ppm nisin. Nisin is currently applied in foods to prevent spoilage induced by bacteria but not by mold. The results of the present study indicate that a combined treatment of nisin in small concentrations of PA might be useful in preventing mold damage in certain foods and stored grain.

Evaluation of gaseous allyl isothiocyanate against the growth of mycotoxigenic fungi and mycotoxin production in corn stored for 6 months

2018 ◽  
Vol 98 (14) ◽  
pp. 5235-5241 ◽  
Author(s):  
Tiago M Nazareth ◽  
Jessica A F Corrêa ◽  
Anne C S M Pinto ◽  
Juliano B Palma ◽  
Giuseppe Meca ◽  
...  
Keyword(s):  
Allyl Isothiocyanate ◽  
Mycotoxin Production ◽  
Mycotoxigenic Fungi

Devices containing allyl isothiocyanate against the growth of spoilage and mycotoxigenic fungi in mozzarella cheese

2018 ◽  
Vol 42 (11) ◽  
pp. e13779
Author(s):  
B. L. Tracz ◽  
K. Bordin ◽  
K. C. P. Bocate ◽  
R. V. Hara ◽  
C. Luz ◽  
...  
Keyword(s):  
Allyl Isothiocyanate ◽  
Mozzarella Cheese ◽  
Mycotoxigenic Fungi

scholarly journals Detection of Mycotoxigenic Fungi and Mycotoxins in Poultry Feed of Balochistan Pakistan

2021 ◽  
Vol 72 (1) ◽  
pp. 2741
Author(s):  
FA MAJEED ◽  
A AKBAR ◽  
M SHAFEE ◽  
H SADIA ◽  
S IQBAL ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Aflatoxin Production ◽  
Poultry Feed ◽  
Direct Examination ◽  
Toxic Metabolites ◽  
Mycotoxigenic Fungi ◽  
Poultry Farmers ◽  
Layer Chromatography ◽  
Feed Samples

Mycotoxins are secondary toxic metabolites of fungi, commonly associated to agriculture commodities. Aspergillus, Fusarium, Penicillium and Claviceps genera are the most common mycotoxigenic fungi and the most important types of mycotoxin are Aflatoxins, Zearalenone, Ochratoxin, Fumonisins and Trichothecenes Mycotoxins can be carcinogenic, cytotoxic, mutagenic, teratogenic, neurotoxic, nephrotoxic, estrogenic and immunosuppressant. This study was conducted to examine the presence of mycotoxigenic fungi and mycotoxins in poultry feed used by the poultry farmers of Balochistan. Samples (n=100) were analyzed and found contaminated with fungi such as Aspergillus, Mucor, Rhizopus and Fusarium genera, amongst them Aspergillus species was observed highly prevalent (89%). Aflatoxin production in culture plates were initially screened with the help of ammonia hydroxide vapor test, and subsequently analyzed with the help of AgraStrip®. The Aflatoxins (77%) and Fumonisins (92%) were found in direct examination of feed samples. Toxins presence was also confirmed with the help of ELISA using AgraQuant® kits. Thin Layer Chromatography (TLC) and High-Performance Liquid Chromatography (HPLC) were used for characterization and quantification of aflatoxins. It was concluded that the feed samples were abundantly contaminated with aflatoxins and fumonisins and the fungi responsible for their production. This contaminated feed is one of the reasons for economic lossesfor poultry farmers of the region.

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