Serological and molecular detection of Toxoplasma gondii
 in sheep and goats in Kashan, Central Iran

Abstract Objective Toxoplasmosis, caused by Toxoplasma gondii, infects humans by consuming infected raw or undercooked meat and foods harboring mature oocysts. In this study, we assessed the prevalence of T. gondii in sheep and goats coming from central Iran. After completing the questionnaire, about one gram of liver or diaphragm tissue was taken as a sample from 90 sheep and 90 goats slaughtered in Yazd Province and stored at – 20 ºC. DNA extraction was done, and then T. gondii was detected using nested PCR. Results This study indicated that the prevalence of T. gondii in all slaughtered animals was 11.6% (21 of 180), including 14.4% (13/90) in sheep and 8.8% (8/90) in goats. The infection rates in liver and diaphragm samples were 12.2% (11/90) and 11.1% (10/90), respectively (p = 0.8163). The infection rate in animals older than one was 16.3% (15/92), and it was 6.8% (6/88) in animals under one year of age. Therefore, no significant differences were found (p = 0.475). Infection rates were 19.5% (18/92) in males and 3.4% (3/88) in females (p = 0.0007). In conclusion, the infection rates of toxoplasmosis in livestock in this area are almost high, and therefore, it is necessary to design appropriate prevention programs to control the disease.

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Molecular Detection of Toxoplasma gondii in the Slaughter Sheep and Goats from Shandong Province, Eastern China

Vector-Borne and Zoonotic Diseases ◽

10.1089/vbz.2019.2488 ◽

2020 ◽

Vol 20 (3) ◽

pp. 193-196 ◽

Cited By ~ 3

Author(s):

Kang Ai ◽

Cui-Qin Huang ◽

Jing-Jing Guo ◽

Hua Cong ◽

Shen-Yi He ◽

...

Keyword(s):

Toxoplasma Gondii ◽

Molecular Detection ◽

Eastern China ◽

Shandong Province ◽

Sheep And Goats

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Molecular detection of Toxoplasma gondii in the slaughter sheep and goats from North India

Veterinary Parasitology ◽

10.1016/j.vetpar.2017.05.009 ◽

2017 ◽

Vol 241 ◽

pp. 35-38 ◽

Cited By ~ 7

Author(s):

Deepali Kalambhe ◽

J.P.S. Gill ◽

Balbir Bagicha Singh

Keyword(s):

Toxoplasma Gondii ◽

Molecular Detection ◽

North India ◽

Sheep And Goats

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Toxoplasma gondii in Milk of Human and Goat from the Desert Area in Central Iran

Iranian Journal of Parasitology ◽

10.18502/ijpa.v16i4.7873 ◽

2021 ◽

Author(s):

Esmat Mohammadi Khamsian ◽

Bahador Hajimohammadi ◽

Gilda Eslami ◽

Mohammad Hossein Fallahzadeh ◽

Saeede Sadat Hosseini

Keyword(s):

Statistical Analysis ◽

Toxoplasma Gondii ◽

Human Milk ◽

Molecular Detection ◽

Goat Milk ◽

Central Iran ◽

Salting Out ◽

Milk Samples ◽

Geographical Regions ◽

Significant Difference

Background: Toxoplasma gondii, an obligate intracellular protozoan, causes toxoplasmosis. The aim of this study was molecular detection of T. gondii in breast and goat milk samples by the molecular method in the central Iran. Methods: Totally, 300 human' and 200 goats' milk samples were collected randomly from different regions of central Iran in 2018. DNA extraction was performed by the salting-out method. Molecular detection of the parasite was done by nested-PCR using the specific primer pairs. Statistical analysis was performed by SPSS 23 using descriptive and Chi-square tests. Results: Out of 300 human milk samples, 1 sample (0.3%) was infected with T. gondii. Out of 200 samples of goat milk, 11 samples (5.5%) showed infection with T. gondii. The frequency of infection in goat's milk samples was 4.36% in the south and west, 1.9% in northern regions, and 2% in eastern regions of the province. The statistical analysis showed no significant difference between toxoplasmosis and different geographical regions in the province. Conclusion: Because of the popularity of the goat milk and the transfection probability with the milk to humans, it is recommended to boil milk prior to use. Furthermore, case contamination of T. gondii in the human milk sample showed one of the important paths for infection transmission, which requires further studies.

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