Effects of different areca nut chewing habits on the gut microbiota of mice: High‐throughput sequencing analysis

2018 ◽  
Vol 39 (1) ◽  
Author(s):  
Ziwei Zhao ◽  
Zhongkun Wu ◽  
Chuanhua Wang ◽  
Huan Cheng ◽  
Shuhan Yi ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Sequencing Analysis ◽  
Areca Nut

High-Throughput Sequencing Analysis Reveals Correlations between Host Phylogeny, Gut Microbiota, and Habitat of Wild Frogs from a Mountainous Area

Copeia ◽  
2019 ◽  
Vol 107 (1) ◽  
pp. 131 ◽  
Author(s):  
Yilin Shu ◽  
Pei Hong ◽  
Qiang Yu ◽  
Gang Wang ◽  
Jihui Zhang ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Mountainous Area ◽  
Sequencing Analysis ◽  
Host Phylogeny

scholarly journals Hydroxychloride Trace Minerals Have a Positive Effect on Growth Performance, Carcass Quality and Impact Ileal and Cecal Microbiota in Broiler Chickens

2020 ◽  
Author(s):  
Sandra J.A. van Kuijk ◽  
Yanming Han ◽  
Ana Isabel Garcia-Ruiz ◽  
Ana Rodiles
Keyword(s):  
Body Weight ◽  
Gut Microbiota ◽  
Growth Performance ◽  
High Throughput ◽  
Broiler Chickens ◽  
High Throughput Sequencing ◽  
Trace Minerals ◽  
Carcass Quality ◽  
Sequencing Analysis ◽  
Breast Meat

Abstract Background: The objective was to study the effect of hydroxychloride trace minerals (HTM) on growth performance, carcass quality and gut microbiota of broiler chickens in comparison to sulphate trace minerals (STM). In total 1440 male Ross 308 day-old chicks were divided over 12 replicate pens with 30 birds each per treatment. Four different treatments were tested according to a 2×2 factorial study design, where the animals received a three phase diet containing either inorganic Zn from sulphates or Zn from HTM in high (80 ppm Zn) or low Zn dosage (20 ppm Zn). In all treatments 15 ppm Cu was added from the same mineral source as the Zn. Body weight and feed intake were measured on day 0, 10, 27 and 34, while carcass and breast meat yields were measured at the end of the study (day 34). In addition, high-throughput sequencing analysis was performed in digesta samples from ileum and cecum to study the gut microbiome (day 34). Results: The results showed an improved (P<0.05) body weight of broiler chickens fed HTM, regardless of Zn level, on day 27, while on day 34 this effect remained as a tendency (P= 0.0542). In the overall study period, birds fed HTM had a higher (P<0.05) average daily gain and average daily feed intake when compared to birds fed STM. The mineral source did not affect the carcass characteristics, however, feeding 80 ppm Zn resulted in a significantly higher (P=0.0171) breast meat yield, regardless of source. High-throughput sequencing analysis of the microbiota revealed a higher microbial diversity in the ileum and cecum of HTM fed birds compared to STM fed birds. Taxonomical differences were mainly found in the cecum, specifically between the group fed high and low Zn levels from HTM. This is in line with the mineral contents observed in the cecal digesta. Comparing both groups fed 80 ppm Zn, the HTM group had more Streptococcaceae, Streptococcus, Clostridia, Weissella and Leuconostocaceae compared to the STM group. Conclusions: HTM improved growth performance of the birds; and the source and level of Zn modulated the gut microbiota communities in broilers differentially.

scholarly journals Effects of Andrographolide on Mouse Intestinal Microflora Based on High-Throughput Sequence Analysis

2021 ◽  
Vol 8 ◽  
Author(s):  
Haigang Wu ◽  
Xian Wu ◽  
Li Huang ◽  
Chongmei Ruan ◽  
Jinni Liu ◽  
...  
Keyword(s):  
Microbial Community ◽  
Gut Microbiota ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Bacterial Resistance ◽  
Microbial Community Composition ◽  
Intestinal Flora ◽  
Sequencing Analysis ◽  
Beneficial Bacteria ◽  
Intestinal Microbes

The intestinal flora is a micro-ecosystem that is closely linked to the overall health of the host. We examined the diversity and abundance of intestinal microorganisms in mice following the administration of andrographolide, a component of the Chinese medical herb Andrographis paniculata. Administration of andrographolide produces multiple beneficial effects including anti-inflammatory, antiviral and antibacterial effects but whether it directly influences the gut microbiota is not known. This study investigated whether the oral administration of andrographolide influences the intestinal microbiota and was compared with amoxicillin treatment as a positive control and water only as a negative control. We examined 21 cecal samples and conducted a high-throughput sequencing analysis based on V3-V4 variable region of the 16S rDNA genes. We found that the diversity and abundance of mouse gut microbiota decreased in direct proportion with the amoxicillin dose whereas andrographolide administration did not affect intestinal microbial community structure. The composition of intestinal microbes following andrographolide treatment was dominated by the Firmicutes while Bacteroidetes dominated the amoxicillin treatment group compared with the negative controls. Specifically, the f__Lachnospiraceae_ Unclassified, Lachnospiraceae_ NK4A136_group and Ruminococcaceae_ UCG-014 were enriched with andrographolide administration while Bacteroides, Klebsiella and Escherichia-Shigella significantly increased in the amoxicillin test groups. Amoxicillin administration altered the microbial community composition and structure by increasing the proportion of pathogenic to beneficial bacteria whereas andrographolide administration led to increases in the proportions and abundance of beneficial bacteria. This study provides a theoretical basis for finding alternatives to antibiotics to decrease bacterial resistance and restore intestinal floral imbalances.

scholarly journals Hydroxychloride trace minerals have a positive effect on growth performance, carcass quality and impact ileal and cecal microbiota in broiler chickens

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Sandra J. A. van Kuijk ◽  
Yanming Han ◽  
Ana Isabel Garcia-Ruiz ◽  
Ana Rodiles
Keyword(s):  
Body Weight ◽  
Gut Microbiota ◽  
Growth Performance ◽  
High Throughput ◽  
Broiler Chickens ◽  
High Throughput Sequencing ◽  
Trace Minerals ◽  
Carcass Quality ◽  
Sequencing Analysis ◽  
Breast Meat

Abstract Background The objective was to study the effect of hydroxychloride trace minerals (HTM) on growth performance, carcass quality and gut microbiota of broiler chickens in comparison to sulphate trace minerals (STM). In total 1440 male Ross 308 day-old chicks were divided into 12 replicate pens with 30 birds each per treatment. Four different treatments were tested according to a 2 × 2 factorial study design, where the animals received a three phase diet containing either inorganic Zn from sulphates or Zn from HTM in high (80 mg/kg Zn) or low Zn dosage (20 mg/kg Zn). In all treatments 15 mg/kg Cu was added from the same mineral source as the Zn. Body weight and feed intake were measured on day 0, 10, 27 and 34, while carcass and breast meat yields were measured at the end of the study (day 34). In addition, high-throughput sequencing analysis was performed in digesta samples from ileum and cecum to study the gut microbiome (day 34). Results The results showed an improved (P < 0.05) body weight of broiler chickens fed HTM, regardless of Zn level, on day 27, while on day 34 this effect remained as a tendency (P = 0.0542). In the overall study period, birds fed HTM had a higher (P < 0.05) average daily gain and average daily feed intake when compared to birds fed STM. The mineral source did not affect the carcass characteristics, however, feeding 80 mg/kg Zn resulted in a significantly higher (P = 0.0171) breast meat yield, regardless of source. High-throughput sequencing analysis of the microbiota revealed a higher microbial diversity in the ileum and cecum of HTM fed birds compared to STM fed birds. Taxonomical differences were mainly found in the cecum, specifically between the group fed high and low Zn levels from HTM. This correlated with the mineral contents observed in the cecal digesta. Comparing both groups fed 80 mg/kg Zn, the HTM group had more Streptococcaceae, Streptococcus, Clostridia, Weissella and Leuconostocaceae compared to the STM group. Conclusions HTM improved growth performance of broiler chickens; and the source and level of Zn modulated the gut microbiota communities in broilers differentially.

scholarly journals Hydroxychloride trace minerals have a positive effect on growth performance, carcass quality and impact ileal and cecal microbiota in broiler chickens

2020 ◽  
Author(s):  
Sandra van Kuijk ◽  
Yanming Han ◽  
Ana Isabel Garcia-Ruiz ◽  
Ana Rodiles
Keyword(s):  
Body Weight ◽  
Gut Microbiota ◽  
Growth Performance ◽  
High Throughput ◽  
Broiler Chickens ◽  
High Throughput Sequencing ◽  
Trace Minerals ◽  
Carcass Quality ◽  
Sequencing Analysis ◽  
Breast Meat

Abstract Background: The objective was to study the effect of hydroxychloride trace minerals (HTM) on growth performance, carcass quality and gut microbiota of broiler chickens in comparison to sulphate trace minerals (STM). In total 1440 male Ross 308 day-old chicks were divided into 12 replicate pens with 30 birds each per treatment. Four different treatments were tested according to a 2×2 factorial study design, where the animals received a three phase diet containing either inorganic Zn from sulphates or Zn from HTM in high (80 mg/kg Zn) or low Zn dosage (20 mg/kg Zn). In all treatments 15 mg/kg Cu was added from the same mineral source as the Zn. Body weight and feed intake were measured on day 0, 10, 27 and 34, while carcass and breast meat yields were measured at the end of the study (day 34). In addition, high-throughput sequencing analysis was performed in digesta samples from ileum and cecum to study the gut microbiome (day 34).Results: The results showed an improved (P<0.05) body weight of broiler chickens fed HTM, regardless of Zn level, on day 27, while on day 34 this effect remained as a tendency (P=0.0542). In the overall study period, birds fed HTM had a higher (P<0.05) average daily gain and average daily feed intake when compared to birds fed STM. The mineral source did not affect the carcass characteristics, however, feeding 80 mg/kg Zn resulted in a significantly higher (P=0.0171) breast meat yield, regardless of source. High-throughput sequencing analysis of the microbiota revealed a higher microbial diversity in the ileum and cecum of HTM fed birds compared to STM fed birds. Taxonomical differences were mainly found in the cecum, specifically between the group fed high and low Zn levels from HTM. This correlated with the mineral contents observed in the cecal digesta. Comparing both groups fed 80 mg/kg Zn, the HTM group had more Streptococcaceae, Streptococcus, Clostridia, Weissella and Leuconostocaceae compared to the STM group.Conclusions: HTM improved growth performance of broiler chickens ; and the source and level of Zn modulated the gut microbiota communities in broilers differentially.

Cryptosporidium parvum-Infected Neonatal Mice Show Gut Microbiota Remodelling Using High-Throughput Sequencing Analysis: Preliminary Results

2019 ◽  
Vol 64 (2) ◽  
pp. 268-275 ◽  
Author(s):  
Mohamed Mammeri ◽  
Aurélie Chevillot ◽  
Myriam Thomas ◽  
Christine Julien ◽  
Eric Auclair ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
High Throughput ◽  
Cryptosporidium Parvum ◽  
High Throughput Sequencing ◽  
Sequencing Analysis ◽  
Preliminary Results ◽  
Neonatal Mice

scholarly journals High-throughput sequencing analysis of microbial community diversity in response to indica and japonica bar-transgenic rice paddy soils

PLoS ONE ◽  
2019 ◽  
Vol 14 (9) ◽  
pp. e0222191 ◽  
Author(s):  
Meidan He ◽  
Jiachao Zhang ◽  
Linbo Shen ◽  
Lixin Xu ◽  
Wenjie Luo ◽  
...  
Keyword(s):  
Microbial Community ◽  
Transgenic Rice ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Rice Paddy ◽  
Paddy Soils ◽  
Community Diversity ◽  
Sequencing Analysis ◽  
Microbial Community Diversity ◽  
Rice Paddy Soils

scholarly journals High-Throughput Sequencing Analysis of the Actinobacterial Spatial Diversity in Moonmilk Deposits

Antibiotics ◽  
2018 ◽  
Vol 7 (2) ◽  
pp. 27 ◽  
Author(s):  
Marta Maciejewska ◽  
Magdalena Całusińska ◽  
Luc Cornet ◽  
Delphine Adam ◽  
Igor Pessi ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Spatial Diversity ◽  
Sequencing Analysis
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