Transcriptomic, proteomic, and biochemical analyses reveal a novel neuritogenesis mechanism of Naja naja venom α‐elapitoxin post binding to TrkA receptor of rat pheochromocytoma cells

2020 ◽  
Vol 155 (6) ◽  
pp. 612-637
Author(s):  
Taufikul Islam ◽  
Munmi Majumder ◽  
Bhargab Kalita ◽  
Atanu Bhattacharjee ◽  
Rupak Mukhopadhyay ◽  
...  
Keyword(s):  
Trka Receptor ◽  
Naja Naja ◽  
Pheochromocytoma Cells ◽  
Biochemical Analyses

Visualization of the Platelet-Plasma Interface

1977 ◽  
Vol 35 ◽  
pp. 494-495
Author(s):  
D. C. Brindley ◽  
M. McGill
Keyword(s):  
Platelet Rich Plasma ◽  
Coagulation Factors ◽  
Platelet Membrane ◽  
Rabbit Platelet ◽  
Surface Coat ◽  
Special Relationship ◽  
Routine Method ◽  
Embedding Technique ◽  
Biochemical Analyses ◽  
Adsorptive Properties

Morphological and cytochemical studies of platelets have reported a surface coat, or glycocalyx, external to the plasma membrane (1). Biochemical analyses have likewise confirmed the highly adsorptive properties of platelets as transporters of coagulation factors (2). However, visualization of the platelet membrane by conventional EM procedures does not reflect this special relationship between the platelet and its plasma environment. By the routine method of alcohol-propylene oxide dehydration for Epon embedding, the lipid bilayer nature of the platelet membrane appears similar to other blood cells (Fig. 1). A new rapid embedding technique using dimethoxypropane (DMP) as dehydrating agent (13) has permitted ultrastructural analyses of the surface features of the platelet-plasma interface.Aliquots of human or rabbit platelet-rich plasma (PRP) were added to equal volumes of 6% glutaraldehyde in Millonig's buffer at 37° for 45 minutes, rinsed in buffer and postfixed in 1% osmium in Millonig's buffer for 45 minutes.

Morphological and biochemical analyses of changes in rat hepatocytes related to wine and ethanol consumption

1984 ◽  
Vol 42 ◽  
pp. 232-233
Author(s):  
S.M. Geyer ◽  
C.L. Mendenhall ◽  
J.T. Hung ◽  
E.L. Cardell ◽  
R.L. Drake ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Enzyme Activity ◽  
Malic Enzyme ◽  
Smooth Endoplasmic Reticulum ◽  
Rat Hepatocytes ◽  
Mature Male ◽  
Treatment Groups ◽  
Malic Enzyme Activity ◽  
Biochemical Analyses

Thirty-three mature male Holtzman rats were randomly placed in 3 treatment groups: Controls (C); Ethanolics (E); and Wine drinkers (W). The animals were fed synthetic diets (Lieber type) with ethanol or wine substituted isocalorically for carbohydrates in the diet of E and W groups, respectively. W received a volume of wine which provided the same gram quantity of alcohol consumed by E. The animals were sacrificed by decapitation after 6 weeks and the livers processed for quantitative triglycerides (T3), proteins, malic enzyme activity (MEA), light microscopy (LM) and electron microscopy (EM). Morphometric analysis of randomly selected LM and EM micrographs was performed to determine organellar changes in centrilobular (CV) and periportal (PV) regions of the liver. This analysis (Table 1) showed that hepatocytes from E were larger than those in C and W groups. Smooth endoplasmic reticulum decreased in E and increased in W compared to C values.

Nerve growth factor (NGF)-dependent kinase activities in PC12 pheochromocytoma cells

1988 ◽  
Vol 117 (4_Suppl) ◽  
pp. S51
Author(s):  
ANKE-PEGGY HOLTORF ◽  
K. UNSICKER ◽  
H.-D. HOFMANN
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Nerve Growth ◽  
Pheochromocytoma Cells ◽  
Pc12 Pheochromocytoma Cells

Discovery and Study of the Binding Epitopes of Novel Urease Inhibitors by STD-NMR Spectroscopy and Biochemical Analyses

2013 ◽  
Vol 10 (6) ◽  
pp. 515-521 ◽  
Author(s):  
Atia-tul- Wahab ◽  
Ajmal Khan ◽  
Bishnu P. Marasini ◽  
M. Arif Lodhi ◽  
Atta-ur- Rahman ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Urease Inhibitors ◽  
Std Nmr ◽  
Biochemical Analyses

Paracrine Mechanisms Involved in Mesenchymal Stem Cell Differentiation into Cardiomyocytes

2019 ◽  
Vol 14 (1) ◽  
pp. 9-13 ◽  
Author(s):  
Maryam Farzaneh ◽  
Fatemeh Rahimi ◽  
Masoumeh Alishahi ◽  
Seyed E. Khoshnam
Keyword(s):  
Myocardial Infarction ◽  
Ventricular Dysfunction ◽  
Stem Cell Differentiation ◽  
Myocardial Cells ◽  
Coronary Artery Diseases ◽  
Low Cardiac Output ◽  
Mesenchymal Stem Cell Differentiation ◽  
Proliferation And Differentiation ◽  
The World ◽  
Biochemical Analyses

Cardiovascular disease (CVD) is one of the world-wide healthcare problem that involves the heart or blood vessels. CVD includes myocardial infarction and coronary artery diseases (CAD). Dysfunctional myocardial cells are leading causes of low cardiac output or ventricular dysfunction after cardiac arrest and may contribute to the progression of CVD which could not generate new cardiomyocytes in human adult heart. The mesenchymal stem cells (MSCs) which are present in adult marrow can self-renew and have the capacity of differentiation into multiple types of cells including cardiomyocytes. Recent biochemical analyses greatly revealed that several regulators of MSCs, such as HGF, PDGF, Wnt, and Notch-1 signaling pathways have been shown to be involved in the proliferation and differentiation into cardiomyocytes. Preclinical studies are paving the way for further applications of MSCs in the repair of myocardial infarction. In this study, we discuss and summarize the paracrine mechanisms involved in MSCs differentiation into cardiomyocytes.

Pheochromocytoma cells PC12 contain binding sites for CART (cocaine- and amphetamine-regulated transcript)

2007 ◽  
Author(s):  
Jana Maixnerová ◽  
Darja Blokešová ◽  
Resha Matyšková ◽  
Renata Haugvicová ◽  
Eva Šloncová ◽  
...  
Keyword(s):  
Binding Sites ◽  
Pheochromocytoma Cells
Sign in / Sign up

Export Citation Format

Share Document