Genetic variation of infection degrees to blast disease among rice genotypes

2021 ◽  
Author(s):  
Manjunath Hubballi ◽  
Abhay K. Pandey ◽  
Rajesha Giriyappa
2015 ◽  
Vol 6 ◽  
Author(s):  
Teresa B. De Leon ◽  
Steven Linscombe ◽  
Glenn Gregorio ◽  
Prasanta K. Subudhi

2020 ◽  
Vol 6 (1) ◽  
pp. 38 ◽  
Author(s):  
Apinya Longya ◽  
Sucheela Talumphai ◽  
Chatchawan Jantasuriyarat

Rice blast disease is caused by the ascomycete fungus Pyricularia oryzae and is one of the most destructive rice diseases in the world. The objectives of this study were investigating various fungal morphological characteristics and performing a phylogenetic analysis. Inter-simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) markers were used to examine the genetic variation of 59 rice blast fungus strains, including 57 strains collected from different fields in Thailand and two reference strains, 70-15 and Guy11. All isolates used in this study were determined to be P. oryzae by internal transcribed spacer (ITS) sequence confirmation. A total of 14 ISSR primers and 17 pairs of SRAP primers, which produced clear and polymorphic bands, were selected for assessing genetic diversity. A total of 123 polymorphic bands were generated. The similarity index value for the strains ranged from 0.25 to 0.95. The results showed that the blast fungus population in Thailand has both morphological and genetic variations. A high level of genetic variation, or genome adaptation, is one of the fungal mechanisms that could overcome host resistance to avoid host recognition. Results from this research study could bring substantial benefits and ultimately help to understand the blast fungal pathogen genome and the population structure in Thai blast fungus.


2014 ◽  
Vol 6 (11) ◽  
pp. 153-159 ◽  
Author(s):  
A. Ogunbayo S. ◽  
K. Ojo D. ◽  
A. Sanni K. ◽  
G. Akinwale M. ◽  
Toulou B. ◽  
...  

2018 ◽  
Vol 9 ◽  
Author(s):  
Marie Noelle Ndjiondjop ◽  
Kassa Semagn ◽  
Mounirou Sow ◽  
Baboucarr Manneh ◽  
Arnaud C. Gouda ◽  
...  

2017 ◽  
Vol 5 (4) ◽  
pp. 505-510
Author(s):  
Tirtha Raj Rijal ◽  
Govinda Bahadur Hamal ◽  
Purushhotam Jha ◽  
Keshab Babu Koirala

Blast disease is considered as a major limiting factor in the global rice production because of its wide distribution and destructiveness and it has been causing significant yield loss in all rice growing areas of Nepal. Host resistance is the most desirable means of managing blast, especially in developing countries. Considering the importance of this disease field screening experiment was conducted to identify resistant rice genotypes against this disease. A total of 314 and 346 rice genotypes with resistant (Sabitri) and susceptible checks (Sankarika) were evaluated under epyphytotic conditions during 2016 and 2017 summer seasons at Rampur, respectively.  During 2016 disease severity varied from 1 to 9 and only five genotypes;   Sabitri, IR 12L 110, WAS122-IDSA14-WASB-FKRI, IR 10F 559and IR 10F 616 were resistant, 30 moderately resistant, 150 susceptible and 129 highly susceptible against blast disease. Similarly during 2016 out of 346 genotypes, 23 resistant as ARIZE SWIFT GOLD, IR95784-21-1-1-2, NR2169-10-4-1-1-1-1-1-2, NR2169-10-2-3-1-1-1-1-1, NR2181-165-1-1-1-1-1-1-1, NR2167-48-5-1-2-1-1, NR2171-2-1-1-3-1-1-2, NR2170-5-5-1-6-1-1-3-1, NR2170-31-1-1-5-1-1-1-1, NR2167-41-1-1-3-1, NR2172-34-1-1-1-1-1-1-1, Sabitri, IR82589-B-B-114-3, IR79913-B-238-3-3, IR93823-36, IR08L 152, IR82589-B-B-51-4, IR09F 434, IR55423-01,  IR94391-131-353-19-B-1-1-1-1-1, NR2154-8-1-1-1-1-1, NR 2124-43-3-1-1-1, NR2160-68-1-1-1-1-1., 72 moderately resistant, 191 susceptible and 155 were highly susceptible. Most of the highly susceptible genotypes were knocked down at the time of disease scoring.Int. J. Appl. Sci. Biotechnol. Vol 5(4): 505-510


2020 ◽  
Vol 3 (2) ◽  
pp. 276-286
Author(s):  
Pratiksha Sharma ◽  
Prem Bahadur Magar ◽  
Suraj Baidya ◽  
Ram Baran Yadaw

Blast, caused by Pyriculariagrisea (Sacc.) is the most destructive disease of rice in Nepal. To identify the sources of leaf blast resistance in rice genotypes, a field experiment was conducted under natural epiphytotic condition at National Plant Pathology Research Centre (NPPRC), Khumaltar, Lalitpur, Nepal during summer season in 2018 and 2019.A total of 128 rice genotypes in 2018 and 291during 2019 including resistant check (Sabitri) and susceptible check (Shankharika/Mansuli) were tested. Field experiment was conducted in single rod row design. Leaf blast disease assessment was done according to 0-9 scale. During 2018, 59 entries were highly resistant (Score 0), 34 resistant (Score 1), 26 moderately resistant (Score 2-3), 5 were moderately susceptible (Score 4-5), 4 susceptible (Score 6-7) and none of them were highly susceptible (Score 8-9) to leaf blast. Similarly, in 2019, 6 lines were highly resistant, 70 resistant, 196 moderately resistant, 15 lines were moderately susceptible, 4 susceptible and none of them were highly susceptible to the disease. Only, one genotype NR2179-82-2-4-1-1-1-1 (Score 1) was found resistant in both years. Similarly, genotype NR2182-22-1-3-1-1-1 (Score 2-3) was found moderately resistant. Some of the genotypes were found resistant in 2018 which become moderately resistant in 2019, they were NR2180-20-2-5-1-1-1-1, IR97135-8-3-1-3, IR98786-13-1-2-1, NR2181-139-1-3-1-1-1-1, and IR13F402. So, findings of these resistant and moderately resistant genotypes could be used in resistant source for the development of leaf blast resistant rice varieties through hybridization in future.


2020 ◽  
Vol 16 (4) ◽  
pp. 633-639
Author(s):  
Nguyen Van Cuu ◽  
Nguyen Van Khiem ◽  
Pham Xuan Hoi

Most of the rice cultivars exhibit suspension of growth when submerged to overcome the reduced availability of oxygen. When the situation continues, majority of the cultivars unable to recover after the flood recedes. However, there are fortunately some rice genotypes that can withstand such submerged condition for up to two weeks by adapting two totally opposite mechanisms. One type of cultivars elongates enormously at a very short span of time and the leaves come above the water level. In the second type, they remain under water without any growth. Cultivars of both types tolerate the submergence but the first category easily lodges when flood water recede. In those lines, yields are reduced drastically. In this study, we focus on characterize the genetic variation at the Sub1 locus and to associate its relevance, if any, to submergence tolerance among the deep water landraces. As a first step, seeds of some rice cultivars collected from North-east Indian regions were initially selected for the characterization of genetic variation. The PCR based analysis involving several genes known to be associated with submergence tolerance did not reveal much difference. However, Southern hybridization revealed certain differences between submergence tolerant and susceptible cultivars. Although we did not notice major difference with regard to Sub1 genes when tried with EcoRI and BamHI, differences were noticed with adh1 and RAmy3C genes. Representative, Southern analysis showed the genetic variation among the deep-water cultivars as compared to Swarna and Sub1-Swarna. It is possible that deep-water rice cultivars may not differ in their genome at Sub1 locus but they respond through SNORKEL genes under submergence.


2015 ◽  
Vol 44 (4) ◽  
pp. 405-412 ◽  
Author(s):  
Mst. Tuhina-Khatun ◽  
M. M. Hanafi ◽  
M. Y. Wong ◽  
M. Y. Rafii

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