Unravelling of Chlorella associated bacterial load, diversity, and their imputed functions at high and low yield conditions through metagenome sequencing

2021 ◽  
Author(s):  
Madhubalaji Chegu Krishnamurthi ◽  
R Sarada ◽  
Sandeep N Mudliar
Keyword(s):  
Bacterial Load ◽  
Metagenome Sequencing ◽  
Yield Conditions

5-Benzylidene-4-Oxazolidinones are Synergistic with Antibiotics for the Treatment of Staphylococcus Aureus Biofilms

2019 ◽  
Author(s):  
Bram Frohock ◽  
Jessica M. Gilbertie ◽  
Jennifer C. Daiker ◽  
Lauren V. Schnabel ◽  
Joshua Pierce
Keyword(s):  
Staphylococcus Aureus ◽  
Human Health ◽  
Matrix Model ◽  
Bacterial Load ◽  
Collagen Matrix ◽  
Resistance Mechanisms ◽  
Novel Therapeutics ◽  
Innovative Treatment ◽  
Antibiotic Action ◽  
Biofilm Infection

<div>The failure of frontline antibiotics in the clinic is one of the most serious threats to human health and requires a multitude of novel therapeutics and innovative treatment approaches to curtail the growing crisis. In addition to traditional resistance mechanisms resulting in the lack of efficacy of many antibiotics, most chronic and recurring infections are further made tolerant to antibiotic action by the presence of biofilms. Herein, we report an expanded set of 5-benzylidene-4-oxazolidinones that are able to inhibit the formation of Staphylococcus aureus biofilms, disperse preformed biofilms and in combination with common antibiotics are able to significantly reduce the bacterial load in a robust collagen-matrix model of biofilm infection.</div>

Assessment of Bacterial Load of Some Fresh and Packed Fruit Juices in Arba Minch Town, Ethiopia

2019 ◽  
Vol 09 (03) ◽  
Author(s):  
Bikila Wedajo ◽  
Awel Kadire
Keyword(s):  
Bacterial Load ◽  
Fruit Juices

Influence of Anthropogenic Pressures on Groundwater Quality from a Rural Area

2017 ◽  
Vol 68 (8) ◽  
pp. 1744-1748
Author(s):  
Catalina Stoica ◽  
Gabriela Geanina Vasile ◽  
Alina Banciu ◽  
Daniela Niculescu ◽  
Irina Lucaciu ◽  
...  
Keyword(s):  
Water Quality ◽  
Anthropogenic Activities ◽  
Groundwater Resources ◽  
Bacterial Load ◽  
Animal Husbandry ◽  
Anthropogenic Factors ◽  
Contamination Level ◽  
Anthropogenic Pressures ◽  
Limit Values ◽  
Physico Chemical

During the past few decades, the anthropogenic activities induced worldwide changes in the ecological systems, including the aquatic systems. This work analysed the contamination level of groundwater resources from a rural agglomeration (Central-Western part of Prahova County) by biological and physico-chemical approaches. The study was performed during the autumn of 2016 on several sampling sites (four drilling wells, depth higher than 100 m supplying three villages; two wells lower than 10 m depth and one spring). The water quality was evaluated by comparison with the limit values of the drinking water quality legislation (Law no.458/2002) and the Order 621/2014 (applicable to all groundwater bodies of Romania). The results showed that phenols and metals (iron and manganese) exceeded the threshold values in all sampling sites. Moreover, the anthropogenic factors including agriculture, use of fertilizers, manures, animal husbandry led to an increase of the bacterial load, particularly at wells sites.

Negative Pressure Therapy in the Surgical Treatment of Diabetic Foot

2017 ◽  
Vol 68 (7) ◽  
pp. 1648-1651
Author(s):  
Bogdan Mihnea Ciuntu ◽  
Ciprian Vasiluta ◽  
Robert Negru ◽  
Roxana Hultoana ◽  
Roxana Ciuntu ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Negative Pressure ◽  
Diabetic Foot ◽  
Bacterial Load ◽  
Abdominal Wall Defects ◽  
Negative Pressure Therapy ◽  
Simultaneous Application ◽  
Average Hospital ◽  
Pressure Therapy ◽  
Days Of Therapy

The study aims to assess the significance of negative pressure therapy in the treatment of diabetic foot.The objectives intend to evaluate the healing time required after applying the method and the functional consequences for the patient. A prospective study was conducted on a sample of 37 patients with diabetic foot were monitored their clinical course between September 2014 - April 2017, following negative pressure therapy. There were used vacuum assisted closure devices (VAC � -Hartman) in order to apply negative pressure to the wound, while complying with specified settings (negative pressure, time of use of a kit) in accordance with patients� outcome.There were monitored changes in wound size (planimetric and volumetric measurement), their bacterial load and duration of treatment. Healing was obtained in all cases, to an average hospital stay of 27.3 days and 8 days of therapy application.The negative result of microbial cultures was obtained after an average of 6.45 days by simultaneous application of negative pressure and antibiotic treatment according to the antibiogram. Skin grafts were necessary to close the defect in 4 cases. After basic treatment of the wound, auxiliary methods such as negative pressure contribute to the healing.In patients with diabetic foot who were required surgical intervention, the use of negative pressure therapy yielded a significant benefit in the preservation of the affected limb, after minimal excision.The results we obtained throughout our experience recommend use of NPTW technique as indication for abdominal wall surgery in closing abdominal wall defects, compartment syndrome and surgical site infection after prosthetic mesh.

Wastewater Treatment by Stabilization Ponds with and without Macrophytes under Arid Climate

1993 ◽  
Vol 28 (10) ◽  
pp. 177-181 ◽  
Author(s):  
L. Mandi ◽  
N. Ouazzani ◽  
K. Bouhoum ◽  
A. Boussaid
Keyword(s):  
Bacterial Load ◽  
Fecal Coliforms ◽  
Arid Climate ◽  
Organic Load ◽  
Experimental Systems ◽  
Helminth Eggs ◽  
Stabilization Ponds ◽  
Treated Effluents ◽  
Summer Time ◽  
Influent Flow

This paper presents a comparative study of two experimental systems, stabilization ponds without macrophytes and macrophytic ponds, to purify wastewater under arid climate of Marrakesh. Organic load (COD, TSS), bacterial load (SF, CF) and parasitical load (helminth eggs) are significantly reduced in both systems. Even though water loss was more important at the macrophytic ponds (evapotranspiration reaches 60% of influent flow), this system shows generally the best efficiency in purifying wastewater. In summer time, TSS and COD depletion is better on macrophytic ponds (TSS: 95%, COD: 87%). The stabilization ponds are more efficient to reduce nutrients: NH4+: 72%. PO4: 63% at the same period. Fecal streptococci and fecal coliforms are reduced more in stabilization ponds (CF: 99.4%, SF: 99.7%) than macrophytic ponds (CF: 96.2%, SF: 94.7%). No helminth eggs were found in the effluent from either system. The two systems ensure optimal removal of parasites allowing the eventual reuse of the treated effluents for agricultural purposes.

Microbial stresses on human umbilical cord stem cells

2020 ◽  
Vol 15 ◽  
Author(s):  
Didem Kart ◽  
Betül Çelebi-Saltik
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Umbilical Cord ◽  
Bacterial Load ◽  
Early Period ◽  
Stress Factors ◽  
Human Umbilical Cord ◽  
Antibacterial Effects ◽  
Hematopoietic Stem ◽  
Umbilical Cord Stem Cells

: Umbilical cord and cord blood are acceptable as attractive sources of mesenchymal and hematopoietic stem cells, since their collection is non-invasive, painless, and does not evoke the ethical concerns. Microorganism-stem cell interaction plays an important role in stem cell self-renewal, differentiation, secretion profile and death. In the literature, few researchers are examining the relationship between pathogenic and commensal bacteria with umbilical cord-derived Mesenchymal Stem Cells (MSCs). These relationships vary depending on the bacterial load and the presence of the immune cell in the environment. Several bacterial pathogens act in the regenerative capacity of MSCs by changing their phenotype, development and viability due to several stress factors that are created by a microorganism such as hypoxia, oxidative stress, etc. On the other hand, the anti-inflammatory and antibacterial effects of MSCs were shown and these phenomena increased when the number of bacteria was high but decreased in the presence of low amounts of bacteria. The antibacterial effects of MSCs increased in the early period of infection, while their effects were decreased in the late period with high inflammatory response and bacterial load. In this review, we discussed the microbial stresses on human umbilical cord stem cells.

Sign in / Sign up

Export Citation Format

Share Document