Characterization and application of a flow system for in vitro multispecies oral biofilm formation

2013 ◽  
Vol 49 (3) ◽  
pp. 323-332 ◽  
Author(s):  
V. Blanc ◽  
S. Isabal ◽  
M. C. Sánchez ◽  
A. Llama-Palacios ◽  
D. Herrera ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Flow System ◽  
Oral Biofilm

Killing activity of LFchimera on periodontopathic bacteria and multispecies oral biofilm formation in vitro

2017 ◽  
Vol 33 (9) ◽  
Author(s):  
Sopita Ruangcharoen ◽  
Waraporn Suwannarong ◽  
Marie Rossini Carmela T. Lachica ◽  
Jan G. M. Bolscher ◽  
Kamran Nazmi ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Oral Biofilm ◽  
Periodontopathic Bacteria ◽  
Killing Activity

scholarly journals Identification of Streptococcus sanguinis Genes Required for Biofilm Formation and Examination of Their Role in Endocarditis Virulence

2008 ◽  
Vol 76 (6) ◽  
pp. 2551-2559 ◽  
Author(s):  
Xiuchun Ge ◽  
Todd Kitten ◽  
Zhenming Chen ◽  
Sehmi P. Lee ◽  
Cindy L. Munro ◽  
...  
Keyword(s):  
Infective Endocarditis ◽  
Dental Plaque ◽  
Biofilm Formation ◽  
Bacterial Colonization ◽  
Abundant Species ◽  
Oral Biofilm ◽  
Streptococcus Sanguinis ◽  
Streptococcal Species

ABSTRACT Streptococcus sanguinis is one of the pioneers in the bacterial colonization of teeth and is one of the most abundant species in the oral biofilm called dental plaque. S. sanguinis is also the most common viridans group streptococcal species implicated in infective endocarditis. To investigate the association of biofilm and endocarditis, we established a biofilm assay and examined biofilm formation with a signature-tagged mutagenesis library of S. sanguinis. Four genes that have not previously been associated with biofilm formation in any other bacterium, purB, purL, thrB, and pyrE, were putatively identified as contributing to in vitro biofilm formation in S. sanguinis. By examining 800 mutants for attenuation in the rabbit endocarditis model and for reduction in biofilm formation in vitro, we found some mutants that were both biofilm defective and attenuated for endocarditis. However, we also identified mutants with only reduced biofilm formation or with only attenuation in the endocarditis model. This result indicates that the ability to form biofilms in vitro is not associated with endocarditis virulence in vivo in S. sanguinis.

scholarly journals Influence of topography and hydrophilicity on initial oral biofilm formation on microstructured titanium surfaces in vitro

2011 ◽  
Vol 23 (3) ◽  
pp. 301-307 ◽  
Author(s):  
A. Almaguer-Flores ◽  
R. Olivares-Navarrete ◽  
M. Wieland ◽  
L. A. Ximénez-Fyvie ◽  
Z. Schwartz ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Oral Biofilm ◽  
Titanium Surfaces

scholarly journals Experimental Models of Oral Biofilms Developed on Inert Substrates: A Review of the Literature

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Lopez-Nguyen Darrene ◽  
Badet Cecile
Keyword(s):  
Biofilm Formation ◽  
Bacterial Species ◽  
Experimental Models ◽  
Pathogenic Microorganisms ◽  
Review Of The Literature ◽  
Complex Environment ◽  
Oral Biofilm ◽  
Mucosal Surfaces ◽  
Oral Biofilms

The oral ecosystem is a very complex environment where more than 700 different bacterial species can be found. Most of them are organized in biofilm on dental and mucosal surfaces. Studying this community is important because a rupture in stability can lead to the preeminence of pathogenic microorganisms, causing dental decay, gingivitis, or periodontitis. The multitude of species complicates biofilm analysis so its reproduction, collection, and counting are very delicate. The development of experimental models of dental biofilms was therefore essential and multiplein vitrodesigns have emerged, each of them especially adapted to observing biofilm formation of specific bacteria within specific environments. The aim of this review is to analyze oral biofilm models.

scholarly journals Plant-based oral care product exhibits antibacterial effects on different stages of oral multispecies biofilm development in vitro

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Nadine Kommerein ◽  
Almut Johanna Weigel ◽  
Meike Stiesch ◽  
Katharina Doll
Keyword(s):  
Species Distribution ◽  
Biofilm Formation ◽  
Antibacterial Effect ◽  
Oral Care ◽  
Oral Biofilm ◽  
Care Product ◽  
Planktonic Bacteria ◽  
Multispecies Biofilm ◽  
Biofilm Development

Abstract Background Excessive biofilm formation on surfaces in the oral cavity is amongst the main reasons for severe infection development like periodontitis and peri-implantitis. Mechanical biofilm removal as well as the use of adjuvant antiseptics supports the prevention of pathogenic biofilm formation. Recently, the antibacterial effect of the oral care product REPHA-OS®, based on medicinal plant extracts and essential oils, has been demonstrated on oral pathogens grown on agar plates. In the present study, the effectiveness of the product on medical relevant oral biofilm development should be demonstrated for the first time. Methods An established in vitro oral multispecies biofilm, composed of Streptococcus oralis, Actinomyces naeslundii, Veillonella dispar and Porphyromonas gingivalis, was used to analyze the antibacterial effect of different REPHA-OS® concentrations on planktonic bacteria, biofilm formation and mature biofilms. It was quantified using metabolic activity assays and live/dead fluorescence staining combined with three-dimensional confocal laser-scanning microscopy. Additionally, effects on species distribution inside the biofilm were assessed by means of quantitative real-time PCR. Results REPHA-OS® showed statistically significant antimicrobial effects on all stages of biofilm development: a minimal inhibitory concentration of 5% could be detected for both, for planktonic bacteria and for biofilm formation. Interestingly, only a slightly higher concentration of 10% was necessary to completely kill all bacteria in mature biofilms also. In contrast, an influence on the biofilm matrix or the species distribution could not be observed. The effect could be attributed to the herbal ingredients, not to the contained ethanol. Conclusion The strong antibacterial effect of REPHA-OS® on different stages of oral biofilm development strengthens its application as an alternative adjuvant in oral care therapies.

scholarly journals Laser microtextured titanium implant surfaces reduce in vitro and in situ oral biofilm formation

PLoS ONE ◽  
2018 ◽  
Vol 13 (9) ◽  
pp. e0202262 ◽  
Author(s):  
Andrei C. Ionescu ◽  
Eugenio Brambilla ◽  
Francesco Azzola ◽  
Marco Ottobelli ◽  
Gaia Pellegrini ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Titanium Implant ◽  
Oral Biofilm

scholarly journals In Vitro Oral Biofilm Formation on Triclosan-Coated Sutures in the Absence and Presence of Additional Antiplaque Treatment

2011 ◽  
Vol 69 (4) ◽  
pp. 980-985 ◽  
Author(s):  
Sebastiaan Venema ◽  
Frank Abbas ◽  
Betsy van de Belt-Gritter ◽  
Henny C. van der Mei ◽  
Henk J. Busscher ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Oral Biofilm

scholarly journals In vitro Characterization of Biofilm Formation in Prevotella Species

2021 ◽  
Vol 2 ◽  
Author(s):  
Shurooq Zakariya Albaghdadi ◽  
Jenan Bader Altaher ◽  
Hana Drobiova ◽  
Radhika G. Bhardwaj ◽  
Maribasappa Karched
Keyword(s):  
Biofilm Formation ◽  
Biochemical Characterization ◽  
Dnase I ◽  
Oral Biofilm ◽  
Biofilm Inhibition ◽  
Biofilm Growth ◽  
In Vitro Characterization ◽  
Biofilm Detachment ◽  
Green Fluorescent

Background: Periodontitis, a chronic inflammatory oral infection is the outcome of disturbances in the homeostasis of the oral biofilm microbiota. A number of studies have found the occurrence of Prevotella species in elevated levels in periodontitis compared to healthy subjects. Even though different aspects of Prevotella as part of oral biofilm have been studied, in vitro biofilms formed by these species have not been characterized systematically. The objective of this study was to characterize biofilms formed by several Prevotella species and further to assess biofilm inhibition and detachment of preformed biofilms.Methods: Biofilms were grown in 24-well plates containing brucella broth in anaerobic conditions for 3 days, and were quantified using crystal violet staining. Images of SYTO 9 Green fluorescent stained biofilms were captured using confocal microscopy. Biofilm inhibition and detachment by proteinase and DNase I was tested. The biochemical characterization included quantification of proteins and DNA in the biofilms and biofilm-supernatants.Results:Prevotella loescheii, Prevotella oralis and Prevotella nigrescens showed highest biofilm formation. P. nigrescens formed significantly higher amounts of biofilms than P. loescheii (P = 0.005) and P. oralis (P = 0.0013). Inhibition of biofilm formation was significant only in the case of P. oralis when treated with proteinase (P = 0.037), whereas with DNase I treatment, the inhibition was not significant (P = 0.531). Overall, proteinase was more effective in biofilm detachment than DNase I. Protein and DNA content were higher in biofilm than the supernatant with the highest amounts found in P. nigrescens biofilm and supernatants. P. oralis biofilms appeared to secrete large amounts of proteins extracellularly into the biofilm-supernatants.Conclusion: Significant differences among Prevotella species to form biofilms may imply their variable abilities to get integrated into oral biofilm communities. Of the species that were able to grow as biofilms, DNase I and proteinase inhibited the biofilm growth or were able to cause biofilm detachment.

Bioactivity of Phenolic Compounds Extracted from Strawberry against Formation of Pseudomonas aeruginosa Biofilm

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Baydaa Hussein ◽  
Zainab A. Aldhaher ◽  
Shahrazad Najem Abdu-Allah ◽  
Adel Hamdan
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Phenolic Compounds ◽  
Biofilm Formation ◽  
Opportunistic Infections ◽  
Formation Rate ◽  
Hydrocarbon Chain ◽  
Health Concern ◽  
Gas Chromatography Mass Spectrometry ◽  
Phenolic Contents

Background: Biofilm is a bacterial way of life prevalent in the world of microbes; in addition to that it is a source of alarm in the field of health concern. Pseudomonas aeruginosa is a pathogenic bacterium responsible for all opportunistic infections such as chronic and severe. Aim of this study: This paper aims to provide an overview of the promotion of isolates to produce a biofilm in vitro under special circumstances, to expose certain antibiotics to produce phenotypic evaluation of biofilm bacteria. Methods and Materials: Three diverse ways were used to inhibited biofilm formation of P.aeruginosa by effect of phenolic compounds extracts from strawberries. Isolates produced biofilm on agar MacConkey under certain circumstances. Results: The results showed that all isolates were resistant to antibiotics except sensitive to azithromycin (AZM, 15μg), and in this study was conducted on three ways to detect the biofilm produced, has been detected by the biofilm like Tissue culture plate (TCP), Tube method (TM), Congo Red Agar (CRA). These methods gave a clear result of these isolates under study. Active compounds were analyzed in both extracts by Gas Chromatography-mass Spectrometry which indicate High molecular weight compound with a long hydrocarbon chain. Conclusion: Phenolic compounds could behave as bioactive material and can be useful to be used in pharmaceutical synthesis. Phenolic contents which found in leaves and fruits extracts of strawberries shows antibacterial activity against all strains tested by the ability to reduce the production of biofilm formation rate.

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