A dual functional redox enzyme maturation protein for respiratory and assimilatory nitrate reductases in bacteria

The twin-arginine protein transport (Tat) system is a remarkable molecular machine dedicated to the translocation of fully folded proteins across energy-transducing membranes. Complex cofactor-containing Tat substrates acquire their cofactors prior to export, and substrate proteins actually require to be folded before transport can proceed. Thus, it is very likely that mechanisms exist to prevent wasteful export of immature Tat substrates or to curb competition between immature and mature substrates for the transporter. Here we assess the primary sequence relationships between the accessory proteins implicated in this process during assembly of key respiratory enzymes in the model prokaryote Escherichia coli. For each respiratory enzyme studied, a redox enzyme maturation protein (REMP) was assigned. The main finding from this review was the hitherto unexpected link between the Tat-linked REMP DmsD and the nitrate reductase biosynthetic protein NarJ. The evolutionary link between Tat transport and cofactor insertion processes is discussed.Key words: Tat translocase, twin-arginine leader, hydrogenase, nitrate reductase, TMAO reductase, DMSO reductase, formate dehydrogenase, Tor, Dms, Hya, Hyb, Fdh, Nap.

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An extremely SAD case: structure of a putative redox-enzyme maturation protein fromArchaeoglobus fulgidusat 3.4 Å resolution

Acta Crystallographica Section D Biological Crystallography ◽

10.1107/s0907444906055065 ◽

2007 ◽

Vol 63 (3) ◽

pp. 348-354 ◽

Cited By ~ 17

Author(s):

Olga Kirillova ◽

Maksymilian Chruszcz ◽

Igor A. Shumilin ◽

Tatiana Skarina ◽

Elena Gorodichtchenskaia ◽

...

Keyword(s):

Redox Enzyme ◽

Enzyme Maturation ◽

Maturation Protein ◽

Case Structure

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Identification of Protein-Protein Interactions Between the TatB and TatC Subunits of the Twin-Arginine Translocase System and the Redox Enzyme Maturation Protein Chaperones

Biophysical Journal ◽

10.1016/j.bpj.2013.11.3706 ◽

2014 ◽

Vol 106 (2) ◽

pp. 669a

Author(s):

Lalita Kuzniatsova ◽

Raymond J. Turner

Keyword(s):

Protein Interactions ◽

Protein Protein Interactions ◽

Redox Enzyme ◽

Enzyme Maturation ◽

Protein Chaperones ◽

Maturation Protein ◽

Twin Arginine Translocase

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Escherichia Coli Redox Enzyme Maturation Proteins, TorD and DmsD Interact with GTP as Shown by Native Page Assays

Biophysical Journal ◽

10.1016/j.bpj.2009.12.1318 ◽

2010 ◽

Vol 98 (3) ◽

pp. 243a

Author(s):

Vy A. Tran ◽

Raymond J. Turner

Keyword(s):

Escherichia Coli ◽

Redox Enzyme ◽

Native Page ◽

Enzyme Maturation

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Dual-Function, Light Switchable Cobalt Catalysis via Active Site Metamorphosis

10.26434/chemrxiv.11353106.v1 ◽

2019 ◽

Author(s):

Enrico Bergamaschi ◽

Frédéric Beltran ◽

Christopher Teskey

Keyword(s):

Visible Light ◽

Active Site ◽

Catalytic Site ◽

Dual Function ◽

Catalytic Systems ◽

Hydride Complex ◽

Dual Functional ◽

Multiple Processes ◽

Olefin Migration

Switchable catalysis offers opportunities to control the rate or selectivity of a reaction via a stimulus such as pH or light. However, few examples of switchable catalytic systems that can facilitate multiple processes exist. Here we report a rare example of such dual-functional, switchable catalysis. Featuring an easily prepared, bench-stable cobalt(I) hydride complex in conjunction with pinacolborane, we can completely alter the reaction outcome between two widely employed transformations – olefin migration and hydroboration – with visible light as the sole trigger. This dichotomy arises from ligand photodissociation which leads to metamorphosis of the active catalytic site, resulting in divergent mechanistic pathways.

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Faculty Opinions recommendation of Surface display of a redox enzyme and its site-specific wiring to gold electrodes.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.717970464.793469060 ◽

2013 ◽

Author(s):

James Canary ◽

Xiaojian Wang

Keyword(s):

Surface Display ◽

Gold Electrodes ◽

Redox Enzyme ◽

Site Specific

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Dual Functional Interactions of Substance P and Opioids in Nociceptive Transmission: Review and Reconciliation

Analgesia ◽

10.3727/107156998819565974 ◽

1998 ◽

Vol 3 (4) ◽

pp. 259-268 ◽

Cited By ~ 11

Author(s):

Iwona Maszczynska ◽

Andrzej W. Lipkowski ◽

Daniel B. Carr ◽

Richard M. Kream

Keyword(s):

Substance P ◽

Nociceptive Transmission ◽

Functional Interactions ◽

Dual Functional

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Synergy of Dual Functional Sites for Conversion of CO2 in a Cycloaddition Reaction under Solvent-Free Conditions by a Zn(II)-Based Coordination Network with a Ladder Motif

Crystal Growth & Design ◽

10.1021/acs.cgd.0c01703 ◽

2021 ◽

Author(s):

Unnati Patel ◽

Parth Patel ◽

Bhavesh Parmar ◽

Abhishek Dadhania ◽

Eringathodi Suresh

Keyword(s):

Cycloaddition Reaction ◽

Solvent Free ◽

Functional Sites ◽

Coordination Network ◽

Dual Functional ◽

Solvent Free Conditions

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Thermo- and Photoresponsive Behaviors of Dual-Stimuli-Responsive Organogels Consisting of Homopolymers of Coumarin-Containing Methacrylate

Polymers ◽

10.3390/polym13030329 ◽

2021 ◽

Vol 13 (3) ◽

pp. 329

Author(s):

Seidai Okada ◽

Eriko Sato

Keyword(s):

Functional Group ◽

Solution Temperature ◽

Stimuli Responsive ◽

Critical Solution Temperature ◽

Equilibrium Degree ◽

Stimuli Responsive Polymers ◽

Responsive Polymers ◽

Dual Functional ◽

Wide Range ◽

Increasing Temperature

Coumarin-containing vinyl homopolymers, such as poly(7-methacryloyloxycoumarin) (P1a) and poly(7-(2′-methacryloyloxyethoxy)coumarin) (P1b), show a lower critical solution temperature (LCST) in chloroform, which can be controlled by the [2 + 2] photochemical cycloaddition of the coumarin moiety, and they are recognized as monofunctional dual-stimuli-responsive polymers. A single functional group of monofunctional dual-stimuli-responsive polymers responds to dual stimuli and can be introduced more uniformly and densely than those of dual-functional dual-stimuli-responsive polymers. In this study, considering a wide range of applications, organogels consisting of P1a and P1b, i.e., P1a-gel and P1b-gel, respectively, were synthesized, and their thermo- and photoresponsive behaviors in chloroform were investigated in detail. P1a-gel and P1b-gel in a swollen state (transparent) exhibited phase separation (turbid) through a temperature jump and reached a shrunken state (transparent), i.e., an equilibrium state, over time. Moreover, the equilibrium degree of swelling decreased non-linearly with increasing temperature. Furthermore, different thermoresponsive sites were photopatterned on the organogel through the photodimerization of the coumarin unit. The organogels consisting of homopolymers of coumarin-containing methacrylate exhibited unique thermo- and photoresponsivities and behaved as monofunctional dual-stimuli-responsive organogels.

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