Two R2R3 ‐ MYB Genes Cooperatively Control Trichome Development and Cuticular Wax Biosynthesis in Prunus persica

Abstract Petals of the monocot Phalaenopsis aphrodite (Orchidaceae) possess conical epidermal cells on their adaxial surfaces, and a large amount of cuticular wax is deposited on them to serve as a primary barrier against biotic and abiotic stresses. It has been widely reported that subgroup 9A members of the R2R3-MYB gene family, MIXTA and MIXTA-like in eudicots, act to regulate the differentiation of conical epidermal cells. However, the molecular pathways underlying conical epidermal cell development and cuticular wax biosynthesis in monocot petals remain unclear. Here, we characterized two subgroup 9A R2R3-MYB genes, PaMYB9A1 and PaMYB9A2 (PaMYB9A1/2), from P. aphrodite through the transient overexpression of their coding sequences and corresponding chimeric repressors in developing petals. We showed that PaMYB9A1/2 function to coordinate conical epidermal cell development and cuticular wax biosynthesis. In addition, we identified putative targets of PaMYB9A1/2 through comparative transcriptome analyses, revealing that PaMYB9A1/2 act to regulate the expression of cell wall-associated and wax biosynthetic genes. Furthermore, a chemical composition analysis of cuticular wax showed that even-chain n-alkanes and odd-chain primary alcohols are the main chemical constituents of cuticular wax deposited on petals, which is inconsistent with the well-known biosynthetic pathways of cuticular wax, implying a distinct biosynthetic pathway occurring in P. aphrodite flowers. These results reveal that the function of subgroup 9A R2R3-MYB family genes in regulating the differentiation of epidermal cells is largely conserved in monocots and dicots. Furthermore, both PaMYB9A1/2 have evolved additional functions controlling the biosynthesis of cuticular wax.

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Genome-wide identification, functional prediction, and evolutionary analysis of the R2R3-MYB superfamily in Brassica napus

Genome ◽

10.1139/gen-2017-0059 ◽

2017 ◽

Vol 60 (10) ◽

pp. 797-814 ◽

Cited By ~ 23

Author(s):

Ali Hajiebrahimi ◽

Hajar Owji ◽

Shiva Hemmati

Keyword(s):

Brassica Napus ◽

Purifying Selection ◽

Gene Clusters ◽

Regulatory Elements ◽

Post Inoculation ◽

Evolutionary Analysis ◽

Salinity Treatment ◽

Myb Genes ◽

R2r3 Myb ◽

Leptosphaeria Biglobosa

R2R3-MYB transcription factors (TFs) have been shown to play important roles in plants, including in development and in various stress conditions. Phylogenetic analysis showed the presence of 249 R2R3-MYB TFs in Brassica napus, called BnaR2R3-MYB TFs, clustered into 38 clades. BnaR2R3-MYB TFs were distributed on 19 chromosomes of B. napus. Sixteen gene clusters were identified. BnaR2R3-MYB TFs were characterized by motif prediction, gene structure analysis, and gene ontology. Evolutionary analysis revealed that BnaR2R3-MYB TFs are mainly formed as a result of whole-genome duplication. Orthologs and paralogs of BnaR2R3-MYB TFs were identified in B. napus, B. rapa, B. oleracea, and Arabidopsis thaliana using synteny-based methods. Purifying selection was pervasive within R2R3-MYB TFs. Kn/Ks values lower than 0.3 indicated that BnaR2R3-MYB TFs are being functionally converged. The role of gene conversion in the formation of BnaR2R3-MYB TFs was significant. Cis-regulatory elements in the upstream regions of BnaR2R3-MYB genes, miRNA targeting BnaR2R3MYB TFs, and post translational modifications were identified. Digital expression data revealed that BnaR2R3-MYB genes were highly expressed in the roots and under high salinity treatment after 24 h. BnaMYB21, BnaMYB141, and BnaMYB148 have been suggested for improving salt-tolerant B. napus. BnaR2R3-MYB genes were mostly up regulated on the 14th day post inoculation with Leptosphaeria biglobosa and L. maculan. BnaMYB150 is a candidate for increased tolerance to Leptospheria in B. napus.

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Genome-Wide Analysis of the MYB Transcription Factor Superfamily in Physcomitrella patens

International Journal of Molecular Sciences ◽

10.3390/ijms21030975 ◽

2020 ◽

Vol 21 (3) ◽

pp. 975 ◽

Cited By ~ 8

Author(s):

Xiaojun Pu ◽

Lixin Yang ◽

Lina Liu ◽

Xiumei Dong ◽

Silin Chen ◽

...

Keyword(s):

Stress Responses ◽

Physcomitrella Patens ◽

Myb Transcription Factor ◽

Rna Seq ◽

Genome Wide ◽

Terrestrial Environments ◽

Gene Structures ◽

Myb Genes ◽

Myb Proteins ◽

R2r3 Myb

MYB transcription factors (TFs) are one of the largest TF families in plants to regulate numerous biological processes. However, our knowledge of the MYB family in Physcomitrella patens is limited. We identified 116 MYB genes in the P. patens genome, which were classified into the R2R3-MYB, R1R2R3-MYB, 4R-MYB, and MYB-related subfamilies. Most R2R3 genes contain 3 exons and 2 introns, whereas R1R2R3 MYB genes contain 10 exons and 9 introns. N3R-MYB (novel 3RMYB) and NR-MYBs (novel RMYBs) with complicated gene structures appear to be novel MYB proteins. In addition, we found that the diversity of the MYB domain was mainly contributed by domain shuffling and gene duplication. RNA-seq analysis suggested that MYBs exhibited differential expression to heat and might play important roles in heat stress responses, whereas CCA1-like MYB genes might confer greater flexibility to the circadian clock. Some R2R3-MYB and CCA1-like MYB genes are preferentially expressed in the archegonium and during the transition from the chloronema to caulonema stage, suggesting their roles in development. Compared with that of algae, the numbers of MYBs have significantly increased, thus our study lays the foundation for further exploring the potential roles of MYBs in the transition from aquatic to terrestrial environments.

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Genome-wide identification and characterisation of R2R3-MYB genes in sugar beet (Beta vulgaris)

BMC Plant Biology ◽

10.1186/s12870-014-0249-8 ◽

2014 ◽

Vol 14 (1) ◽

Cited By ~ 42

Author(s):

Ralf Stracke ◽

Daniela Holtgräwe ◽

Jessica Schneider ◽

Boas Pucker ◽

Thomas Rosleff Sörensen ◽

...

Keyword(s):

Sugar Beet ◽

Beta Vulgaris ◽

Genome Wide ◽

Myb Genes ◽

R2r3 Myb

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Identification and expression analysis under abiotic stress of the R2R3-MYB genes in Ginkgo biloba L.

Physiology and Molecular Biology of Plants ◽

10.1007/s12298-017-0436-9 ◽

2017 ◽

Vol 23 (3) ◽

pp. 503-516 ◽

Cited By ~ 8

Author(s):

Xinliang Liu ◽

Wanwen Yu ◽

Xuhui Zhang ◽

Guibin Wang ◽

Fuliang Cao ◽

...

Keyword(s):

Abiotic Stress ◽

Expression Analysis ◽

Ginkgo Biloba ◽

Myb Genes ◽

R2r3 Myb

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Genome-Wide Identification of R2R3-MYB Genes and Expression Analyses During Abiotic Stress in Gossypium raimondii

Scientific Reports ◽

10.1038/srep22980 ◽

2016 ◽

Vol 6 (1) ◽

Cited By ~ 47

Author(s):

Qiuling He ◽

Don C. Jones ◽

Wei Li ◽

Fuliang Xie ◽

Jun Ma ◽

...

Keyword(s):

Abiotic Stress ◽

Genome Wide ◽

Gossypium Raimondii ◽

Myb Genes ◽

R2r3 Myb

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Blind Homologous R2R3 Myb Genes Control the Pattern of Lateral Meristem Initiation in Arabidopsis

The Plant Cell ◽

10.1105/tpc.105.038745 ◽

2006 ◽

Vol 18 (3) ◽

pp. 586-597 ◽

Cited By ~ 140

Author(s):

Dörte Müller ◽

Gregor Schmitz ◽

Klaus Theres

Keyword(s):

Lateral Meristem ◽

Myb Genes ◽

R2r3 Myb

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Genome-Wide Analysis of R2R3-MYB Transcription Factors Family in The Autopolyploid Saccharum Spontaneum: An Exploration of Dominance Expression and Stress Response

10.21203/rs.3.rs-199103/v1 ◽

2021 ◽

Author(s):

Yuan Yuan ◽

Xiping Yang ◽

Mengfang Feng ◽

Hongyan Ding ◽

Khan Muhammad Tahir ◽

...

Keyword(s):

Transcription Factors ◽

Stress Response ◽

Gene Family ◽

Expression Analysis ◽

Expression Profiling ◽

Saccharum Spontaneum ◽

Myb Gene ◽

Genome Wide ◽

Myb Genes ◽

R2r3 Myb

Abstract Background: Sugarcane (Saccharum) is the most important sugar crop in the world. As one of the most enriched transcription factor families in plants, MYB genes display a great potential to contribute to sugarcane improvement by trait modification. We have identified the sugarcane MYB gene family at a whole-genome level through systematic evolution analyses and expression profiling. R2R3-MYB is a large subfamily involved in many plant-specific processes. Results: A total of 202 R2R3-MYB genes (356 alleles) were identified in the polyploid Saccharum spontaneum genome and classified into 15 subgroups by phylogenetic analysis. The sugarcane MYB family had more members by a comparative analysis in sorghum and significant advantages among most plants, especially grasses. Collinearity analysis revealed that 70% of the SsR2R3-MYB genes had experienced duplication events, logically suggesting the contributors to the MYB gene family expansion. Functional characterization was performed to identify 56 SsR2R3-MYB genes involved in various plant bioprocesses with expression profiling analysis on 60 RNA-seq databases. We identified 22 MYB genes specifically expressed in the stem, of which MYB43, MYB53, MYB65, MYB78, and MYB99 were validated by qPCR. Allelic expression dominance in the stem was more significant than that in the leaf, implying the differential expression of alleles may be responsible for the high expression of MYB in the stem. MYB169, MYB181, MYB192 were identified as candidate C4 photosynthetic regulators by C4 expression pattern and robust circadian oscillations. Furthermore, stress expression analysis showed that MYB36, MYB48, MYB54, MYB61 actively responded to drought treatment; 19 and 10 MYB genes were involved in response to the sugarcane pokkah boeng and mosaic disease, respectively. Conclusions: A Genome-wide expression analysis demonstrated that SsMYB genes were involved in stem development and stress response. This study largely contributed to understanding the extent to which MYB transcription factors investigate regulatory mechanisms and functional divergence in sugarcane.

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The R2R3-MYB transcription factor family in Taxus chinensis: identification, characterization, expression profiling and posttranscriptional regulation analysis

PeerJ ◽

10.7717/peerj.8473 ◽

2020 ◽

Vol 8 ◽

pp. e8473

Author(s):

Xinling Hu ◽

Lisha Zhang ◽

Iain Wilson ◽

Fenjuan Shao ◽

Deyou Qiu

Keyword(s):

Transcription Factor ◽

Posttranscriptional Regulation ◽

Gene Families ◽

Myb Transcription Factor ◽

Taxus Chinensis ◽

Transcription Factor Family ◽

Myb Genes ◽

And Function ◽

R2r3 Myb

The MYB transcription factor family is one of the largest gene families playing regulatory roles in plant growth and development. The MYB family has been studied in a variety of plant species but has not been reported in Taxus chinensis. Here we identified 72 putative R2R3-MYB genes in T. chinensis using a comprehensive analysis. Sequence features, conversed domains and motifs were characterized. The phylogenetic analysis showed TcMYBs and AtMYBs were clustered into 36 subgroups, of which 24 subgroups included members from T. chinensis and Arabidopsis thaliana, while 12 subgroups were specific to one species. This suggests the conservation and specificity in structure and function of plant R2R3-MYBs. The expression of TcMYBs in various tissues and different ages of xylem were investigated. Additionally, miRNA-mediated posttranscriptional regulation analysis revealed that TcMYBs were the targets of miR858, miR159 and miR828, suggesting the posttranscriptional regulation of MYBs is highly conserved in plants. The results provide a basis for further study the role of TcMYBs in the regulation of secondary metabolites of T. chinensis.

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