Insight into the photosynthetic apparatus in evergreen and deciduous European oaks during autumn senescence using OJIP fluorescence transient analysis

Plant Biology ◽  
2013 ◽  
Vol 16 (4) ◽  
pp. 801-808 ◽  
Author(s):  
V. Holland ◽  
S. Koller ◽  
W. Brüggemann
Keyword(s):  
Transient Analysis ◽  
Photosynthetic Apparatus ◽  
Fluorescence Transient ◽  
Autumn Senescence ◽  
Ojip Fluorescence Transient ◽  
Insight Into

scholarly journals Chlorophyll Fluorescence Transient Analysis in Alternanthera tenella Colla Plants Grown in Nutrient Solution with Different Concentrations of Copper

2013 ◽  
Vol 5 (8) ◽  
Author(s):  
Cristina C. Cuchiara ◽  
Ilda Mariclei C. Silva ◽  
Emanuela G. Martinazzo ◽  
Eugenia Jacira B. Braga ◽  
Marcos Antonio Bacarin ◽  
...  
Keyword(s):  
Chlorophyll Fluorescence ◽  
Nutrient Solution ◽  
Transient Analysis ◽  
Fluorescence Transient ◽  
Chlorophyll Fluorescence Transient

scholarly journals Morphophysiological responses of Billbergia zebrina Lindl. (Bromeliaceae) in function of types and concentrations of carbohydrates during conventional in vitro culture

2020 ◽  
Vol 26 (1) ◽  
pp. 18-34 ◽  
Author(s):  
Elizangela Rodrigues Santos ◽  
João Paulo Rodrigues Martins ◽  
Luiz Carlos de Almeida Rodrigues ◽  
Andreia Barcelos Passos Lima Gontijo ◽  
Antelmo Ralph Falqueto
Keyword(s):  
Photosystem Ii ◽  
In Vitro Culture ◽  
Leaf Anatomy ◽  
Culture Medium ◽  
Stomatal Density ◽  
Photosynthetic Apparatus ◽  
Fluorescence Transient ◽  
Chlorophyll A Fluorescence Transient ◽  
Concentration Interval

Abstract When propagated in vitro, explants receive all the nutrients needed for their growth, including carbohydrates, from the culture medium. However, it is not well understood how the type and concentration of carbohydrates can affect the functioning of the photosynthetic apparatus (particularly photosystem II) of these plants. The aim was to assess the morphophysiological responses of Billbergia zebrina plants in function of sources and concentrations of carbohydrates during in vitro culture. Side shoots of plants previously established in vitro were individualized and transferred to a culture medium containing fructose, glucose or sucrose in four concentrations (0, 15, 30 or 45 g L−1). After growth for 55 days, the chlorophyll a fluorescence transient, leaf anatomy and growth were analyzed. The concentration and type of carbohydrate employed during in vitro culture did not decrease the photosynthetic apparatus performance. However, concentrations above 30 g L−1 led to anatomical modifications, revealing some degree of stress suffered by the plants. When grown in concentrations of 15 and 30 g L−1, irrespective of the carbohydrate used, the plants presented greater stomatal density. The supplementation of the culture medium with monosaccharides caused alterations in the development of the xylem vessels, such as increased number and diameter, allowing adjustment to the microenvironmental conditions. The in vitro conditions influenced the photosynthetic and anatomical responses of plants. The concentration interval from 15 to 30 g L−1 sucrose had a better effect by not causing large changes in the performance of the photosynthetic apparatus and anatomy of plants.

scholarly journals Chlorophyll a Fluorescence Transient and 2-Dimensional Electrophoresis Analyses Reveal Response Characteristics of Photosynthesis to Heat Stress in Malus. ‘Prairifire’

Plants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 1040
Author(s):  
Tao Wang ◽  
Siqian Luo ◽  
Yingli Ma ◽  
Lingyu Li ◽  
Yinfeng Xie ◽  
...  
Keyword(s):  
Heat Stress ◽  
Heat Shock ◽  
Chlorophyll A ◽  
Chlorophyll A Fluorescence ◽  
Photosynthetic Apparatus ◽  
Protective Mechanism ◽  
Response Characteristics ◽  
Fluorescence Transient ◽  
Chlorophyll A Fluorescence Transient ◽  
Dimensional Electrophoresis

Flowering crabapples are a series of precious ornamental woody plants. However, their growth and development are inhibited in the subtropical regions due to the weak photosynthesis under high-temperature environment in the summer. Chlorophyll a fluorescence transient and 2-dimensional electrophoresis (2-DE) analyses were conducted to investigate the response characteristics of photosynthesis under simulated 38 °C heat stress in leaves of Malus. ‘Prairifire’, a spring-red leaf cultivar of flowering crabapple with strong thermal adaptability. In the present study, the net photosynthetic rate (Pn) was significantly decreased during the heat shock process, which showed a similar trend to the stomatal conductance (Gs), indicating a sensitive stomatal behavior to heat stress. Moreover, an efficient reaction center in photosystem II (PSII), and a functionally intact oxygen-evolving complex (OEC) conferred strong photosynthetic adaptability under heat stress. The higher level of transketolase (TK) under 48-h heat shock treatment was considered a protective mechanism of photosynthetic apparatus. However, heat stress inhibited the functions of light harvesting complex II (LHCII), electron transport in PSII, and the levels of key enzymes in the Calvin cycle, which were considered as the reasons causing an increase in the proportion of non-stomatal restrictions.

scholarly journals A review of pressure transient analysis in reservoirs with natural fractures, vugs and/or caves

2020 ◽  
Author(s):  
Isah Mohammed ◽  
Teslim O. Olayiwola ◽  
Murtadha Alkathim ◽  
Abeeb A. Awotunde ◽  
Saad F. Alafnan
Keyword(s):  
Transient Analysis ◽  
Dynamic Pressure ◽  
Flow Dynamics ◽  
Reservoir Pressure ◽  
Natural Fractures ◽  
Pressure Transient Analysis ◽  
Pressure Transient ◽  
Analysis Techniques ◽  
Basic Model ◽  
Insight Into

Abstract A review of the pressure transient analysis of flow in reservoirs having natural fractures, vugs and/or caves is presented to provide an insight into how much knowledge has been acquired about this phenomenon and to highlight the gaps still open for further research. A comparison-based approach is adopted which involved the review of works by several authors and identifying the limiting assumptions, model restrictions and applicability. Pressure transient analysis provides information to aid the identification of important features of reservoirs. It also provides an explanation to complex reservoir pressure-dependent variations which have led to improved understanding and optimization of the reservoir dynamics. Pressure transient analysis techniques, however, have limitations as not all its models find application in naturally fractured and vuggy reservoirs as the flow dynamics differ considerably. Pollard’s model presented in 1953 provided the foundation for existing pressure transient analysis in these types of reservoirs, and since then, several authors have modified this basic model and come up with more accurate models to characterize the dynamic pressure behavior in reservoirs with natural fractures, vugs and/or caves, with most having inherent limitations. This paper summarizes what has been done, what knowledge is considered established and the gaps left to be researched on.

scholarly journals APPLICATION OF OJIP CHLOROPHYLL FLUORESCENCE TRANSIENT ANALYSIS TO STUDY THE POSTHARVEST CHANGES IN PHOTOSINTHETIC APPARATUS IN CUT FOLIAGE SPECIES

2022 ◽  
Vol 2 (3) ◽  
pp. 196-206
Author(s):  
W M U D Wijethunga ◽  
L S H Jayasooriya ◽  
S M J C Subasinghe ◽  
H M P C Kumarihami ◽  
C K Beneragama
Keyword(s):  
Chlorophyll Fluorescence ◽  
Electron Transport ◽  
Quantum Yield ◽  
Transient Analysis ◽  
Photosynthetic Apparatus ◽  
Control Treatment ◽  
Invasive Technique ◽  
Vase Life ◽  
Chlorophyll Contents ◽  
Cut Foliage

Chlorophyll fluorescence (ChlF) is a non-invasive technique that can be potentially used in postharvest research to gain useful information on early responses to postharvest stresses. This study was conducted to validate the application of ChlF transient analysis in determining the postharvest changes in photosynthetic apparatus in three ornamental foliage species, i.e., Cordyline fruticosa ‘Willy’s Gold’ and ‘Rubra’, Dracaena sanderiana ‘White’, and Nephrolepis exaltata. Salicylic acid (100 and 300 mg·L−1), glucose (10 g·L−1), and their combinations were used as holding solutions with control treatment (distilled water) at room temperature (25±2°C). Vase life was evaluated using OJIP analysis. OJIP parameters, i.e., specific energy fluxes per reaction center (ABS/RC, TR/RC, ET/RC, and DI/RC), flux ratios (maximum quantum yield of primary photochemistry-φPo), electron transport efficiency (ψo), and quantum yield of electron transport (φEo), and performance index (PI) were recorded every other day, using a fluorometer (FluorPen 100). Leaf chlorophyll contents of all species and anthocyanin contents of two cordyline cultivars were determined. Data were subjected to ANOVA in a completely randomized design. Mean separation was done by DMRT (p ≤ 0.05). Clear variations in ChlF were observed in every foliage species with the time. OJIP analysis showed species-depended variations. The higher ABS/RC and DI/RC were recorded for D. sanderiana and N. exaltata compared to the PI of those species. At the end of the experiment, the chlorophyll contents were decreased, while anthocyanin contents were increased. Consequently, chlorophyll fluorescence changes in photosynthetic apparatus can be used for the prediction of the postharvest stresses and longevity of cut foliage.

scholarly journals Onset of autumn senescence in High Arctic plants shows similar patterns in natural and experimental snow depth gradients

2021 ◽  
Author(s):  
Friederike Gehrmann ◽  
Camille Ziegler ◽  
Elisabeth J. Cooper
Keyword(s):  
Environmental Gradients ◽  
Growing Season ◽  
High Arctic ◽  
Plant Responses ◽  
Bud Burst ◽  
Delayed Senescence ◽  
Arctic Plants ◽  
Autumn Senescence ◽  
Depth Gradients ◽  
Insight Into

Predicted changes in snow cover and temperature raise uncertainties about how the beginning and the end of the growing season will shift for Arctic plants. Snowmelt timing and temperature are known to affect the timing of bud burst, but their effects on autumn senescence are less clear. To address this, researchers have examined senescence under natural and experimental environmental gradients. However, these approaches address different aspects of plant responses and the extent to which they can be compared is poorly understood. In this study, we show that the effect of snowmelt timing on the timing of autumn senescence in High Arctic plants is the same between a natural and an experimental gradient in three out of four studied species. While the two approaches mostly produce comparable results, they give in combination greater insight into the phenological responses to predicted climate changes. We also showed that a short warming treatment in autumn delayed senescence by 3.5 days in D. octopetala, which is a 10 % extension of the growing season end for this species. Warming treatments have commonly been applied to the whole growing season, but here we show that even isolated autumn warming can be sufficient to affect plant senescence.

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