Bacterial strains integrated with surfactin molecules of Bacillus subtilis MTCC441 enrich nematocidal activity against Meloidogyne incognita

Intracellular serine proteinase was isolated from sporulating cells of Bacillus subtilis Marburg 168 by gramicidin S-Sepharose 4B affinity chromatography. The enzymological characteristics, the amino acid composition and the 19 residues of the N-terminal sequence of the enzyme are reported. The isolated proteinase was closely related to, but not completely identical with, the intracellular serine proteinase of B. subtilis A-50. The divergence between these two intracellular enzymes was less than that between the corresponding extracellular serine proteinases (subtilisins) of types Carlsberg and BPN′!, produced by these bacterial strains. This may be connected with the more strict selection constraints imposed in intracellular enzymes during evolution.

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In vitro antagonistic activity of Bacillus subtilis strains isolated from soils of the Yucatan Peninsula against Macrophomina phaseolina and Meloidogyne incognita

Phyton ◽

10.32604/phyton.2014.83.045 ◽

2014 ◽

Vol 83 (1) ◽

pp. 45-47

Author(s):

Ruiz SE ◽

AJ Crist骲al ◽

RA Reyes ◽

SJ Tun ◽

RA Garc韆 ◽

...

Keyword(s):

Bacillus Subtilis ◽

Meloidogyne Incognita ◽

Yucatan Peninsula ◽

Macrophomina Phaseolina ◽

Antagonistic Activity ◽

Yucatán Peninsula

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Studies on poly-3-hydroxyoctanoate biosynthesis by a consortium of microorganisms

Ovidius University Annals of Chemistry ◽

10.1515/auoc-2016-0009 ◽

2016 ◽

Vol 27 (1) ◽

pp. 44-47 ◽

Cited By ~ 1

Author(s):

Mihaela Carmen Eremia ◽

Irina Lupescu ◽

Mariana Vladu ◽

Maria Petrescu ◽

Gabriela Savoiu ◽

...

Keyword(s):

Bacillus Subtilis ◽

Carbon Source ◽

Pseudomonas Putida ◽

Sole Carbon Source ◽

Bacterial Biomass ◽

Fermentation Medium ◽

Energy Reserve ◽

Bacterial Strains ◽

Sodium Octanoate ◽

Intracellular Energy

Abstract Polyhydroxyalcanoates (PHAs) are specifically produced by a wide variety of bacteria, as an intracellular energy reserve in the form of homo- and copolymers of [R]-β-hydroxyalkanoic acids, depending on the C source used for microorganism growth, when the cells are grown under stressing conditions. In this paper we present microbiological accumulation of poly-3-hydroxyoctanoate (PHO) by using a consortium of bacterial strains, Pseudomonas putida and Bacillus subtilis, in a rate of 3:1, grown on a fermentation medium based on sodium octanoate as the sole carbon source. The experiments performed in the above mentioned conditions led to the following results: from 18.70 g sodium octanoate (7.72 g/L in the fermentation medium) used up during the bioprocess, 3.93-3.96 g/L dry bacterial biomass and 1.834 - 1.884 g/L PHA, containing 85.83 - 86.8% PHO, were obtained.

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Synthesis and Nematocidal Activity of N-Substituted 3-Methyl-1H -pyrazole-4-carboxamide Derivatives Against Meloidogyne incognita

Journal of Heterocyclic Chemistry ◽

10.1002/jhet.3123 ◽

2018 ◽

Vol 55 (4) ◽

pp. 946-950 ◽

Cited By ~ 18

Author(s):

Long Cheng ◽

Zhong-Hua Shen ◽

Tian-Ming Xu ◽

Cheng-Xia Tan ◽

Jian-Quan Weng ◽

...

Keyword(s):

Meloidogyne Incognita ◽

Nematocidal Activity

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EXPRESIÓN DE GENES RELACIONADOS CON PATOGÉNESIS EN CHILE (Capsicum annuum L.) INOCULADO CON Bacillus subtilis Y Meloidogyne enterolobii

Agrociencia ◽

10.47163/agrociencia.v55i3.2418 ◽

2021 ◽

Vol 55 (3) ◽

pp. 261-272

Author(s):

Luis Yobani Gayosso Rosales ◽

Edgar Villar Luna ◽

María Dolores Rodríguez Torres ◽

María Valentina Angoa Pérez ◽

Hortencia Gabriela Mena Violante ◽

...

Keyword(s):

Bacillus Subtilis ◽

Capsicum Annuum ◽

Meloidogyne Incognita ◽

Capsicum Annuum L ◽

Meloidogyne Enterolobii ◽

Expresión Génica

El cultivo de chile (Capsicum annuum L.) destaca por el valor agro-alimenticio alto del producto, aunado a su valor comercial. Los nematodos Meloidogyne incognita y M. enterolobii (Me) afectan al cultivo; M. enterolobii (Me) es el más relevante por su agresividad notable. El estudio de alternativas ecológicas es de interés para control estos fitoparásitos. Los objetivos de esta investigación fueron conocer el efecto de Bacillus subtilis (Bs) (CH90) sobre la expresión de los genes PR-1, PR-5, y PR-12 que codifican proteínas relacionadas con patogénesis en chile cv. California Wonder (Cw) infectado con Me; y evaluar el efecto de Bs sobre agallamiento (A) y producción de huevos (H) del nematodo en raíces de Cw. Dos experimentos independientes (E1 y E2) se establecieron con diseño completamente al azar. En E1 y E2 los tratamientos fueron: Cw inoculado solo con Me (CwMe), Cw con Bs y Me (CwBsMe), Cw solo con Bs (CwBs), y Cw sin inoculación (Cw). En ambos experimentos, el nivel de inóculo de Bs fue 108 UFC mL-1, y para Me fue 500 J2 por planta. En E1 la expresión génica se determinó a 3, 7, y 14 d después de inoculación (DPI) con Me. En E2 las variables A y H se evaluaron 45 DPI con Me. PR-1 y PR-5 se sobre expresaron 3 y 7 DPI en los tratamientos CwBsMe y CwBs, en contraste con CwMe (p≤0.05). A los 14 DPI, los genes en todos los tratamientos tuvieron una expresión menor (p≤0.05). La sobre expresión máxima de PR-12 se registró a 14 DPI en los tratamientos CwBsMe y CwBs (p≤0.05). Las plantas de cv. C. Wonder tratadas con B. subtilis CH90 solo o en combinación con M. enterolobii activaron las rutas de defensa dependientes del ácido salicílico (AS) y jasmónico (AJ) pero dicha activación no afectó la reproducción del nematodo en raíces de chile.

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Potential of Pseudomonas putida, Bacillus subtilis, and their mixture on the management of Meloidogyne incognita, Pectobacterium betavasculorum, and Rhizoctonia solani disease complex of beetroot (Beta vulgaris L.)

Egyptian Journal of Biological Pest Control ◽

10.1186/s41938-019-0174-0 ◽

2019 ◽

Vol 29 (1) ◽

Author(s):

Manzoor R. Khan ◽

Zaki A. Siddiqui

Keyword(s):

Bacillus Subtilis ◽

Rhizoctonia Solani ◽

Pseudomonas Putida ◽

Beta Vulgaris ◽

Meloidogyne Incognita ◽

Root Rot ◽

Growth Parameters ◽

Principal Component ◽

Soft Rot ◽

Disease Complex

AbstractEffects of Pseudomonas putida and Bacillus subtilis alone, and in combinations for the management of Meloidogyne incognita, Pectobacterium betavasculorum, and Rhizoctonia solani disease complex of beetroot (Beta vulgaris L.), were studied. Application of P. putida or B. subtilis to plants with M. incognita or P. betavasculorum or R. solani singly or in combinations caused a significant increase in plant growth parameters and the activities of defense enzymes. A significant increase in chlorophyll fluorescence attributes, viz., Fv/Fm, ɸPSII, qP, NPQ, and ETR were recorded in plants treated with P. putida or B. subtilis over pathogen-inoculated plants. Inoculation of P. putida results in a higher reduction in galling and nematode multiplication than B. subtilis. Maximum reduction in nematode multiplication and galling occurred when a mixture of P. putida and B. subtilis was used. Soft rot and root rot indices were 3 when Pectobacterium betavasculorum and Rhizoctonia solani were inoculated alone. The disease indices were rated 5 when these pathogens and M. incognita were inoculated in combinations. Inoculation of P. putida/B. subtilis with P. betavasculorum or R. solani reduced soft rot and root rot indices to 2 out of 3, while the use of P. putida + B. subtilis reduced indices to 1. Disease indices were reduced to 2–3 out of 5, when P. putida + B. subtilis were used to plants inoculated with two or three pathogens. The principal component analysis showed significant correlations among the various studied attributes. Two principal components explained a total of 86.1 and 93.4% of the overall data variability. Therefore, the use of P. putida together with B. subtilis had the potential for successful management of disease complex of beetroot.

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Bacterial Community Members Increase Bacillus subtilis Maintenance on the Roots of Arabidopsis thaliana

Phytobiomes Journal ◽

10.1094/pbiomes-02-20-0019-r ◽

2020 ◽

Vol 4 (4) ◽

pp. 303-313

Author(s):

Noam Eckshtain-Levi ◽

Susanna Leigh Harris ◽

Reizo Quilat Roscios ◽

Elizabeth Anne Shank

Keyword(s):

Arabidopsis Thaliana ◽

Bacillus Subtilis ◽

Crop Production ◽

Crop Yields ◽

Plant Root ◽

Plant Roots ◽

Plant Growth Promoting Bacteria ◽

Bacterial Strains ◽

Root Microbiome ◽

Over Time

Plant-growth-promoting bacteria (PGPB) are used to improve plant health and promote crop production. However, because some PGPB (including Bacillus subtilis) do not maintain substantial colonization on plant roots over time, it is unclear how effective PGPB are throughout the plant growing cycle. A better understanding of the dynamics of plant root community assembly is needed to develop and harness the potential of PGPB. Although B. subtilis is often a member of the root microbiome, it does not efficiently monoassociate with plant roots. We hypothesized that B. subtilis may require other primary colonizers to efficiently associate with plant roots. We utilized a previously designed hydroponic system to add bacteria to Arabidopsis thaliana roots and monitor their attachment over time. We inoculated seedlings with B. subtilis and individual bacterial isolates from the native A. thaliana root microbiome either alone or together. We then measured how the coinoculum affected the ability of B. subtilis to colonize and maintain on A. thaliana roots. We screened 96 fully genome-sequenced strains and identified five bacterial strains that were able to significantly improve the maintenance of B. subtilis. Three of these rhizobacteria also increased the maintenance of two strains of B. amyloliquefaciens commonly used in commercially available bioadditives. These results not only illustrate the utility of this model system to address questions about plant–microbe interactions and how other bacteria affect the ability of PGPB to maintain their relationships with plant roots but also may help inform future agricultural interventions to increase crop yields. [Formula: see text] Copyright © 2020 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .

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Biodegradation of Vulcanized SBR: A Comparison between Bacillus subtilis, Pseudomonas aeruginosa and Streptomyces sp

Scientific Reports ◽

10.1038/s41598-019-55530-y ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 2

Author(s):

Mostafa Galal Aboelkheir ◽

Priscilla Braga Bedor ◽

Selma Gomes Leite ◽

Kaushik Pal ◽

Romildo Dias Toledo Filho ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Contact Angle ◽

Bacillus Subtilis ◽

Polymeric Chain ◽

Bacterial Strains ◽

Carbon Loss ◽

Streptomyces Sp ◽

Vulcanized Rubber ◽

Recycling Technique ◽

Biological Recycling

AbstractRubber residues present harmful impacts on health and environment, besides wasting valuable and huge amounts of rubber. Biological recycling technique is focused here to minimize this problem. A comparison of the biodegradation effect caused by Bacillus subtilis, Pseudomonas aeruginosa, and Streptomyces sp., separately, on vulcanized SBR-rubber during 4 weeks is reported. The surface and molecular analyses were studied by FTIR-ATR, TGA, DSC, TC and SEM/EDS, in addition to the contact angle and crosslinking tests. B. subtilis, P. aeruginosa, and Streptomyces sp. evoked after 4 weeks a loss in v-SBR crosslinks by 17.15, 10.68 and 43.39% and also in the contact angle with water by 14.10, 12.86 and 15.71%, respectively., if compared to Control samples. FTIR findings indicate that the polymeric chain has been partially consumed causing C-C bonds scission indicating the biodegradation and bio-devulcanization phenomena. The bacterial strains caused a carbon loss by 9.15, 5.97 and 4.55% after one week and 16.09, 16.79 and 18.13% after four weeks for B. subtilis, P. aeruginosa, and Streptomyces sp. mediums, respectively. DSC and EDS results are also promising and highlighting Streptomyces sp. strain as the most effective biodegradative one as an alternative and natural mean of degrading vulcanized rubber residues.

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