A novel fluorescence imaging approach to monitor salt stress-induced modulation of ouabain-sensitive ATPase activity in sunflower seedling roots

2013 ◽  
Vol 150 (4) ◽  
pp. 540-549 ◽  
Author(s):  
Soumya Mukherjee ◽  
Satish Chander Bhatla
Keyword(s):  
Salt Stress ◽  
Atpase Activity ◽  
Fluorescence Imaging ◽  
Imaging Approach ◽  
Seedling Roots ◽  
Sunflower Seedling

Salt stress-induced seedling growth inhibition coincides with differential distribution of serotonin and melatonin in sunflower seedling roots and cotyledons

2014 ◽  
Vol 152 (4) ◽  
pp. 714-728 ◽  
Author(s):  
Soumya Mukherjee ◽  
Anisha David ◽  
Sunita Yadav ◽  
František Baluška ◽  
Satish Chander Bhatla
Keyword(s):  
Salt Stress ◽  
Growth Inhibition ◽  
Seedling Growth ◽  
Differential Distribution ◽  
Seedling Roots ◽  
Sunflower Seedling ◽  
Seedling Growth Inhibition

scholarly journals Nitric oxide modulates polyamine homeostasis in sunflower seedling cotyledons under salt stress

2019 ◽  
Vol 14 (11) ◽  
pp. 1667730 ◽  
Author(s):  
Aditi Tailor ◽  
Rajesh Tandon ◽  
Satish C. Bhatla
Keyword(s):  
Nitric Oxide ◽  
Salt Stress ◽  
Sunflower Seedling

Proteomic analysis of cucumber seedling roots subjected to salt stress

2010 ◽  
Vol 71 (13) ◽  
pp. 1450-1459 ◽  
Author(s):  
Chang-Xia Du ◽  
Huai-Fu Fan ◽  
Shi-Rong Guo ◽  
Takafumi Tezuka ◽  
Juan Li
Keyword(s):  
Salt Stress ◽  
Proteomic Analysis ◽  
Cucumber Seedling ◽  
Seedling Roots

scholarly journals SOS2 Promotes Salt Tolerance in Part by Interacting with the Vacuolar H+-ATPase and Upregulating Its Transport Activity

2007 ◽  
Vol 27 (22) ◽  
pp. 7781-7790 ◽  
Author(s):  
Giorgia Batelli ◽  
Paul E. Verslues ◽  
Fernanda Agius ◽  
Quansheng Qiu ◽  
Hiroaki Fujii ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Atpase Activity ◽  
Ion Transport ◽  
Affinity Purification ◽  
Tandem Affinity Purification ◽  
Mass Spectrometry Analysis ◽  
Transport Activity ◽  
Sos Pathway

ABSTRACT The salt overly sensitive (SOS) pathway is critical for plant salt stress tolerance and has a key role in regulating ion transport under salt stress. To further investigate salt tolerance factors regulated by the SOS pathway, we expressed an N-terminal fusion of the improved tandem affinity purification tag to SOS2 (NTAP-SOS2) in sos2-2 mutant plants. Expression of NTAP-SOS2 rescued the salt tolerance defect of sos2-2 plants, indicating that the fusion protein was functional in vivo. Tandem affinity purification of NTAP-SOS2-containing protein complexes and subsequent liquid chromatography-tandem mass spectrometry analysis indicated that subunits A, B, C, E, and G of the peripheral cytoplasmic domain of the vacuolar H+-ATPase (V-ATPase) were present in a SOS2-containing protein complex. Parallel purification of samples from control and salt-stressed NTAP-SOS2/sos2-2 plants demonstrated that each of these V-ATPase subunits was more abundant in NTAP-SOS2 complexes isolated from salt-stressed plants, suggesting that the interaction may be enhanced by salt stress. Yeast two-hybrid analysis showed that SOS2 interacted directly with V-ATPase regulatory subunits B1 and B2. The importance of the SOS2 interaction with the V-ATPase was shown at the cellular level by reduced H+ transport activity of tonoplast vesicles isolated from sos2-2 cells relative to vesicles from wild-type cells. In addition, seedlings of the det3 mutant, which has reduced V-ATPase activity, were found to be severely salt sensitive. Our results suggest that regulation of V-ATPase activity is an additional key function of SOS2 in coordinating changes in ion transport during salt stress and in promoting salt tolerance.

scholarly journals Overexpression of GSK3-like Kinase 5 (OsGSK5) in rice (Oryza sativa) enhances salinity tolerance in part via preferential carbon allocation to root starch

2017 ◽  
Vol 44 (7) ◽  
pp. 705 ◽  
Author(s):  
Maysaya Thitisaksakul ◽  
Maria C. Arias ◽  
Shaoyun Dong ◽  
Diane M. Beckles
Keyword(s):  
Oryza Sativa ◽  
Salt Stress ◽  
Salinity Tolerance ◽  
Carbon Allocation ◽  
Oryza Sativa L ◽  
Rice Seedling ◽  
Shoot Biomass ◽  
Salt Shock ◽  
Seedling Roots ◽  
Root Starch

Rice (Oryza sativa L.) is very sensitive to soil salinity. To identify endogenous mechanisms that may help rice to better survive salt stress, we studied a rice GSK3-like isoform (OsGSK5), an orthologue of a Medicago GSK3 previously shown to enhance salinity tolerance in Arabidopsis by altering carbohydrate metabolism. We wanted to determine whether OsGSK5 functions similarly in rice. OsGSK5 was cloned and sequence, expression, evolutionary and functional analyses were conducted. OsGSK5 was expressed highest in rice seedling roots and was both salt and sugar starvation inducible in this tissue. A short-term salt-shock (150 mM) activated OsGSK5, whereas moderate (50 mM) salinity over the same period repressed the transcript. OsGSK5 response to salinity was due to an ionic effect since it was unaffected by polyethylene glycol. We engineered a rice line with 3.5-fold higher OsGSK5 transcript, which better tolerated cultivation on saline soils (EC = 8 and 10 dS m–2). This line produced more panicles and leaves, and a higher shoot biomass under high salt stress than the control genotypes. Whole-plant 14C-tracing and correlative analysis of OsGSK5 transcript with eco-physiological assessments pointed to the accelerated allocation of carbon to the root and its deposition as starch, as part of the tolerance mechanism.

scholarly journals The signaling lipid PI(3,5)P2 stabilizes V1–Vo sector interactions and activates the V-ATPase

2014 ◽  
Vol 25 (8) ◽  
pp. 1251-1262 ◽  
Author(s):  
Sheena Claire Li ◽  
Theodore T. Diakov ◽  
Tao Xu ◽  
Maureen Tarsio ◽  
Wandi Zhu ◽  
...  
Keyword(s):  
Salt Stress ◽  
Atpase Activity ◽  
Human Disease ◽  
Proton Pumps ◽  
Disease Phenotypes ◽  
Reversible Dissociation ◽  
Signaling Mechanisms ◽  
Vacuolar Acidification ◽  
Direct Binding

Vacuolar proton-translocating ATPases (V-ATPases) are highly conserved, ATP-driven proton pumps regulated by reversible dissociation of its cytosolic, peripheral V1 domain from the integral membrane Vo domain. Multiple stresses induce changes in V1-Vo assembly, but the signaling mechanisms behind these changes are not understood. Here we show that certain stress-responsive changes in V-ATPase activity and assembly require the signaling lipid phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2). V-ATPase activation through V1-Vo assembly in response to salt stress is strongly dependent on PI(3,5)P2 synthesis. Purified Vo complexes preferentially bind to PI(3,5)P2 on lipid arrays, suggesting direct binding between the lipid and the membrane sector of the V-ATPase. Increasing PI(3,5)P2 levels in vivo recruits the N-terminal domain of Vo-sector subunit Vph1p from cytosol to membranes, independent of other subunits. This Vph1p domain is critical for V1-Vo interaction, suggesting that interaction of Vph1p with PI(3,5)P2-containing membranes stabilizes V1-Vo assembly and thus increases V-ATPase activity. These results help explain the previously described vacuolar acidification defect in yeast fab1∆ and vac14∆ mutants and suggest that human disease phenotypes associated with PI(3,5)P2 loss may arise from compromised V-ATPase stability and regulation.

Waterborne versus Dietary Zinc Accumulation and Toxicity in Daphnia magna: a Synchrotron Radiation Based X-ray Fluorescence Imaging Approach

2011 ◽  
Vol 46 (2) ◽  
pp. 1178-1184 ◽  
Author(s):  
R. Evens ◽  
K. A. C. De Schamphelaere ◽  
B. De Samber ◽  
G. Silversmit ◽  
T. Schoonjans ◽  
...  
Keyword(s):  
Synchrotron Radiation ◽  
Fluorescence Imaging ◽  
Daphnia Magna ◽  
Dietary Zinc ◽  
Zinc Accumulation ◽  
X Ray ◽  
Imaging Approach

scholarly journals Comparative transcriptome analyses of maize seedling root responses to salt stress

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10765
Author(s):  
Xiaoxiang Zhang ◽  
Peng Liu ◽  
Chunyan Qing ◽  
Cong Yang ◽  
Yaou Shen ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Maize Seedling ◽  
Salt Tolerant ◽  
Maize Roots ◽  
Differential Expression Genes ◽  
Salt Response ◽  
Seedling Roots ◽  
Tolerant Line ◽  
Sensitive Line

Salt stress affects crop yield by limiting growth and delaying development. In this study, we constructed 16 transcriptome libraries from maize seedling roots using two maize lines, with contrasting salt tolerance, that were exposed to salt stress for 0, 6, 18 and 36 h. In total, 6,584 differential expression genes (DEGs; 3,669 upregulated, 2,915 downregulated) were induced in the salt-sensitive line and 6,419 DEGs (3,876 upregulated, 2,543 downregulated) were induced in the salt-tolerant line. Several DEGs common to both lines were enriched in the ABA signaling pathway, which was presumed to coordinate the process of maize salt response. A total of 459 DEGs were specifically induced in the salt-tolerant line and represented candidate genes responsible for high salt-tolerance. Expression pattern analysis for these DEGs indicated that the period between 0 and 6 h was a crucial period for the rapid response of the tolerant genes under salt stress. Among these DEGs, several genes, Aux/IAA, SAUR, and CBL-interacting kinase have been reported to regulate salt tolerance. In addition, the transcription factors WRKY, bZIP and MYB acted as regulators in the salt-responsive regulatory network of maize roots. Our findings will contribute to understanding of the mechanism on salt response and provide references for functional gene revelation in plants.

scholarly journals Effects of temperature and irradiance on a benthic microalgal community: A combined two-dimensional oxygen and fluorescence imaging approach

2014 ◽  
Vol 59 (5) ◽  
pp. 1599-1611 ◽  
Author(s):  
Kasper Hancke ◽  
Brian K. Sorell ◽  
Lars Chresten Lund-Hansen ◽  
Morten Larsen ◽  
Torunn Hancke ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Two Dimensional ◽  
Imaging Approach ◽  
Effects Of Temperature ◽  
Microalgal Community
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