scholarly journals An apoplastic fluid extraction method for the characterization of grapevine leaves proteome and metabolome from a single sample

2020 ◽  
Author(s):  
Joana Figueiredo ◽  
Ana Rita Cavaco ◽  
Leonor Guerra‐Guimarães ◽  
Céline Leclercq ◽  
Jenny Renaut ◽  
...  
Keyword(s):  
Extraction Method ◽  
Single Sample ◽  
Fluid Extraction ◽  
Grapevine Leaves ◽  
Apoplastic Fluid

Extraction of oil from uncrushed olives using supercritical fluid extraction method

2014 ◽  
Vol 95 ◽  
pp. 512-518 ◽  
Author(s):  
Awni Al-Otoom ◽  
Sameer Al-Asheh ◽  
Mamdouh Allawzi ◽  
Khalid Mahshi ◽  
Nahawand Alzenati ◽  
...  
Keyword(s):  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Extraction Method ◽  
Fluid Extraction

scholarly journals Are streamflow recession characteristics really characteristic?

2013 ◽  
Vol 17 (2) ◽  
pp. 817-828 ◽  
Author(s):  
M. Stoelzle ◽  
K. Stahl ◽  
M. Weiler
Keyword(s):  
Extraction Method ◽  
Storage Capacity ◽  
Extraction Methods ◽  
Model Parameters ◽  
Catchment Scale ◽  
Model Parameterization ◽  
Recession Curves ◽  
Recession Analysis ◽  
Meso Scale

Abstract. Streamflow recession has been investigated by a variety of methods, often involving the fit of a model to empirical recession plots to parameterize a non-linear storage–outflow relationship based on the dQ/dt−Q method. Such recession analysis methods (RAMs) are used to estimate hydraulic conductivity, storage capacity, or aquifer thickness and to model streamflow recession curves for regionalization and prediction at the catchment scale. Numerous RAMs have been published, but little is known about how comparably the resulting recession models distinguish characteristic catchment behavior. In this study we combined three established recession extraction methods with three different parameter-fitting methods to the power-law storage–outflow model to compare the range of recession characteristics that result from the application of these different RAMs. Resulting recession characteristics including recession time and corresponding storage depletion were evaluated for 20 meso-scale catchments in Germany. We found plausible ranges for model parameterization; however, calculated recession characteristics varied over two orders of magnitude. While recession characteristics of the 20 catchments derived with the different methods correlate strongly, particularly for the RAMs that use the same extraction method, not all rank the catchments consistently, and the differences among some of the methods are larger than among the catchments. To elucidate this variability we discuss the ambiguous roles of recession extraction procedures and the parameterization of the storage–outflow model and the limitations of the presented recession plots. The results suggest strong limitations to the comparability of recession characteristics derived with different methods, not only in the model parameters but also in the relative characterization of different catchments. A multiple-methods approach to investigating streamflow recession characteristics should be considered for applications whenever possible.

Characterization of Soluble Organic Fraction in DPM: Optimization of the Extraction Method

1999 ◽  
Author(s):  
Antonio de Lucas ◽  
Antonio Durán ◽  
Manuel Carmona ◽  
Magín Lapuerta
Keyword(s):  
Extraction Method ◽  
Organic Fraction ◽  
Soluble Organic Fraction

scholarly journals Isolation of Chloramphenicol from Whole Eggs by Supercritical Fluid Extraction with In-Line Collection

1999 ◽  
Vol 82 (6) ◽  
pp. 1334-1339 ◽  
Author(s):  
John W Pensabene ◽  
Walter Fiddler ◽  
Dan J Donoghue
Keyword(s):  
Solvent Extraction ◽  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Extraction Method ◽  
Gas Flow ◽  
Extraction Procedure ◽  
The United States ◽  
Fluid Extraction ◽  
Solvent Extraction Method ◽  
Significant Difference

Abstract Egg consumption, at more than 65 billion per year in the United States, represents a potentially significant source of exposure to drug residues, particularly if the laying hens are treated with antimicrobial compounds or fed a diet containing medicated feed. Residues resulting from the use of chloramphenicol (CAP) is especially problematic if this compound is not used in accordance with national registration, e.g., for the control of Salmonella microorganisms in poultry. The most commonly used methods for the determination of CAP in biological samples require the use of large amounts of organic solvent. As a result, a less solvent intensive supercritical fluid extraction (SFE) method was developed for CAP in whole chicken eggs, and the results were compared with those for a solvent extraction procedure. In the SFE method, the egg sample is extracted with supercritical CO2 (without a modifier) at 10 000 psi (680 bar), 80°C, and an expanded gas flow rate of 3.0 L/min to a total volume of 150 L. The CAP is trapped in-line on a Florisil sorbent bed. The CAP is eluted post-SFE by using the liquid chromatographic mobile phase solvent (water-methanol), and determined on a C8 column with ultraviolet detection at 280 nm. Recovery from eggs fortified at the 10 ppb level (n = 6) was 81.2 ± 4.3%. To obtain eggs containing incurred CAP, hens were given a single daily dose of 75 mg CAP (orally by gelatin capsule) for 2 consecutive days, and the eggs were collected over a 12-day period. The mean value for “normally incurred” CAP in the eggs (n = 17) analyzed by SFE ranged from none detected to 174.5 ppb, with an overall mean of 60.5 ppb, compared with a mean of 60.4 ppb for the solvent extraction method. No significant difference in results was found between methods. However, the SFE method is more rapid, uses less solvent, and gives recoveries similar to those for the solvent extraction method, making it ideal for regulatory monitoring.

The Characterization of Bovine Bone-Derived Hydroxyapatite Isolated Using Novel Non-Hazardous Method

2020 ◽  
Vol 45 ◽  
pp. 49-56
Author(s):  
Aniek Setiya Budiatin ◽  
Samirah ◽  
Maria Apriliani Gani ◽  
Wenny Putri Nilamsari ◽  
Chrismawan Ardianto ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Extraction Method ◽  
Bovine Bone ◽  
X Ray Diffraction ◽  
X Ray ◽  
Ft Ir ◽  
Novel Method ◽  
Scanning Electron

Bovine bone is a considerable source for the production of hydroxyapatite. The recent study reported a novel method to extract hydroxyapatite from bovine bone without producing hazardous residue. The bovine bones were cut and boiled in the opened chamber followed by boiling in pressurized tank. The bones were then soaked into 95% ethanol. Calcination was then conducted in 800°C, 900°C and 1,000°C, for 2 hours. The result was then grinded and sieved. The powder then was characterized using Fourier transform infrared (FT-IR), Scanning electron microscopy (SEM) and X-ray diffraction analysis (XRD) to measure the purity of hydroxyapatite. It is concluded that the hydroxyapatite derived from this process showed 100% purity, resulting 35.34 ± 0.39% w/w from the wet bone weight and 72.3% w/w from the dried weight. The present extraction method has been proven to yield high amount of pure hydroxyapatite as well as reducing the use of hazardous reagent.

Preparation and characterization of para-tertiary-butylphenol formaldehyde resins using dual catalytic-extraction method

2015 ◽  
Vol 87 ◽  
pp. 1-9
Author(s):  
Linfeng Wang ◽  
Wenshi Ma ◽  
Dingfeng Lei ◽  
Dongqiao Zhang
Keyword(s):  
Extraction Method ◽  
Catalytic Extraction
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