Lymph node histology for the assessment of residual neoplastic disease in canine mast cell tumours: does the presence of metachromatic granules always identify mast cells?

2016 ◽  
Vol 15 (4) ◽  
pp. 1119-1121 ◽  
Author(s):  
L. Ressel ◽  
R. Finotello
Keyword(s):  
Mast Cell ◽  
Lymph Node ◽  
Mast Cells ◽  
Neoplastic Disease ◽  
Lymph Node Histology ◽  
Metachromatic Granules

Two populations of mast cells on fibroblast monolayers: correlation of quantitative microscopy and functional activity

1988 ◽  
Vol 91 (1) ◽  
pp. 13-19
Author(s):  
I. Hammel ◽  
H. Shiloh-Rabinovich ◽  
I. Nir
Keyword(s):  
Mast Cell ◽  
Lymph Node ◽  
Mast Cells ◽  
Cultured Cells ◽  
Dense Granules ◽  
Cellular Biochemistry ◽  
Lymph Node Cells ◽  
Embryonic Fibroblast ◽  
Two Populations ◽  
Morphometric Evaluation

We have previously shown that a confluent layer of mature mast cells is obtained when lymph node cells are grown on embryonic fibroblast monolayers. Two populations of mast cells may be observed, depending on treatment of the mice from which the lymph node cells are derived. We report now on the morphometric evaluation of these two mast cell entities, and we correlate this with cellular biochemistry and secretory behaviour. The first type of mast cell is small (265 +/− 20 microns 3). It arises from the embryonic monolayer and the cells have feathers resembling those of connective tissue mast cells. These cultured cells are filled with about 1000 homogeneous electron-dense granules, which usually range in diameter from 0.05 to 0.2 microns. The second type of mast cell arises from precursors originating from the lymph node and they have feathers of mucosal mast cells. These cells are larger (480 +/− 40 microns 3) and contain about 300 heterogeneous granules, which range from 0.1 to 0.8 microns. Both cell entities contain about equal amounts of histamine, serotonin and chymase. Biologically, the two cell entities secrete soluble mediators (histamine and serotonin) at different rates compared to the rate at which they secrete chymase. We suggest that such a pattern of secretion exhibits a form of degranulation that permits the release of freely diffusible mediators that are loosely bound to granules, but only partially permits the secretion of insoluble mediators, which are stored in the granules. Alternatively, there might be a mechanism that rapidly inactivates or binds chymase so that only the vasoamines will be free.

scholarly journals CYTOFLUOROMETRIC STUDY OF MAST CELL POLYANIONS II. ADULT RAT PERITONEAL MAST CELLS REGENERATING AFTER POLYMYXIN TREATMENT

1969 ◽  
Vol 17 (1) ◽  
pp. 56-61 ◽  
Author(s):  
SAM L. MEYER ◽  
ALEX M. SAUNDERS
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Polymyxin B ◽  
Adult Rat ◽  
Peritoneal Mast Cells ◽  
Fetal Rats ◽  
Rat Peritoneal Mast Cells ◽  
Time Changes ◽  
Metachromatic Granules ◽  
And Storage

Mast cells with metachromatic granules are not detectable in rats after polymyxin-B sulfate treatment. The morphologic and staining characteristics of the cells that repopulate the peritoneal cavity resemble those of mast cells of fetal rats in their maturation sequence, except that, in the adult, the sequence requires at least 56 days. During this time changes occur in the competitive staining of mast cells with acridine orange-sodium chloride, indicating that polyanion synthesis and storage in the granules is a multiphasic phenomenon.

scholarly journals ON THE ORIGIN OF HEPARIN

1947 ◽  
Vol 86 (2) ◽  
pp. 107-116 ◽  
Author(s):  
Jean Oliver ◽  
Frank Bloom ◽  
Carmen Mangieri
Keyword(s):  
Mast Cell ◽  
Particulate Matter ◽  
Mast Cells ◽  
Biochemical Study ◽  
Blood Stream ◽  
Cell Tumor ◽  
Mast Cell Tumors ◽  
Precursor Phase ◽  
Mast Cell Tumor ◽  
Metachromatic Granules

1. The spontaneous mast cell tumor of the dog contains heparin. 2. The cytoplasmic particulate content of the tumor mast cells varies with their anaplasia. This conclusion is based on the following findings: (a) in the immature cell of the more malignant tumor the particulate matter appeared in the living cells by phase microscopy to be composed of greyish illdefined particles or as a fine, weakly metachromatic granulation in the fixed and stained preparation; (b) in the mature cells of a relatively benign mast cell tumor, both in the living cell and in stained preparations, the particulate matter occurred in the form of discrete, dense, and strongly metachromatic granules, resembling those of the normal mast cell. 3. The heparin content was large (fifty times that of dog liver) in the growth with mature cells and only moderate (1.7 times) in that with immature cells. 4. Since there may be a great amount of greyish particulate matter (or fine stained granules) in a tumor of relatively low heparin content, it is suggested that this material represents an early or precursor phase in the development of heparin. 5. This possibility and the fact that the blood stream may be invaded by mature tumor mast cells of large heparin content without evident disturbance in the coagulability of the blood suggest the value of a comprehensive biochemical study of the heparin of mast cell tumors.

scholarly journals A study on mast cell variation in neoplastic and non neoplastic disease of uterine cervix

2014 ◽  
Vol 4 (8) ◽  
pp. 658-662 ◽  
Author(s):  
N Mainali ◽  
AK Sihna ◽  
P Upadhyaya ◽  
D Upreti
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Cell Count ◽  
Uterine Cervix ◽  
Statistical Significance ◽  
Neoplastic Disease ◽  
Neoplastic Lesion ◽  
Mast Cell Count ◽  
Cell Variation ◽  
Relationship Of

Background: Mast cells are heterogeneous group of immune cells involved in multiple biological events. The significance of mast cells in uterine tumor surveillance has been studied with conflicting results. The presence of mast cell in tumor has been described as evidence of a host immunologic anti tumor response and if they are abundant the prognosis is good. However in other studies, with the help of different granules of mast cell, it is said to be very closely related with angiogenesis and tumor invasion. The study aims to analyze the histomorphologic changes with special reference to mast cells in different neoplastic and non neoplastic disease of uterine cervix, and also the relationship of the mast cell population with degree of anaplasia and mitotic figures.Materials and methods: Cervical biopsies received in the department of Pathology for HPE were stained with H& E stain and toludine blue for the identification of mast cellResult: Out of a total of 100 cases, 82 were non neoplastic cases with the mean mast cell count of 83.73 and mean age of patient being 44.30 year. Eighteen neoplastic cases were included which had mean mast cell count of 13.5 and mean age of 49.5 year.Conclusion: Mast cell was found to be highest in non Neoplastic lesion with increase count in polypoidal cervicitis. There was a statistical significance variation between mast cell count in neoplastic and non Neoplastic disease of the cervix. However,role of age in mast cell count was least significant.DOI: http://dx.doi.org/10.3126/jpn.v4i8.11594 Journal of Pathology of Nepal; Vol.4,No. 8 (2014) 658-662

Cytochemical analysis of mast cell granules after poly-dl-lysine action

1978 ◽  
Vol 36 (2) ◽  
pp. 278-279
Author(s):  
R. Courtoy ◽  
L.J. Simar ◽  
J. Christophe
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Chemical Compounds ◽  
Cellular Membrane ◽  
Thin Sections ◽  
Organic Bases ◽  
Ferric Thiocyanate ◽  
Cytochemical Analysis ◽  
Mast Cell Granule ◽  
Amine Liberation

Several chemical compounds induce amine liberation from mast cells but do not necessarily provoque the granule expulsion. For example, poly-dl-lysine induces modifications of the cellular membrane permeability which promotes ion exchange at the level of mast cell granules. Few of them are expulsed but the majority remains in the cytoplasm and appears less dense to the electrons. A cytochemical analysis has been performed to determine the composition of these granules after the polylysine action.We have previously reported that it was possible to demonstrate polyanions on epon thin sections using a cetylpyridinium ferric thiocyanate method. Organic bases are selectively stained with cobalt thiocyanate and the sulfhydryle groups are characterized with a silver methenamine reaction. These techniques permit to reveal the mast cell granule constituents, i.e. heparin, biogenic amines and basic proteins.

Magnesium and sulfur in mast cell and basophil granules of lower vertebrates in relation with histamine

1992 ◽  
Vol 50 (2) ◽  
pp. 1596-1597
Author(s):  
Kenichi Takaya
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Mast Cell ◽  
Mast Cells ◽  
Tree Frog ◽  
X Ray ◽  
Transmission Electron ◽  
Scanning Transmission ◽  
Fresh Frozen ◽  
Ultrathin Sections

Mast cell and basophil granules of the vertebrate contain heparin or related sulfated proteoglycans. Histamine is also present in mammalian mast cells and basophils. However, no histamine is detected in mast cell granules of the amphibian or fish, while it is shown in those of reptiles and birds A quantitative x-ray microanalysis of mast cell granules of fresh frozen dried ultrathin sections of the tongue of Wistar rats and tree frogs disclosed high concentrations of sulfur in rat mast cell granules and those of sulfur and magnesium in the tree frog granules. Their concentrations in tree frog mast cell granules were closely correlated (r=0.94).Fresh frozen dried ultrathin sections and fresh air-dried prints of the tree frog tongue and spleen and young red-eared turtle (ca. 6 g) spleen and heart blood were examined by a quantitative energy-dispersive x-ray microanalysis (X-650, Kevex-7000) for the element constituents of the granules of mast cells and basophils. The specimens were observed by transmission electron microscopy (TEM) (80-200 kV) and followed by scanning transmission electron microscopy (STEM) under an analytical electron microscope (X-650) at an acceleration voltage of 40 kV and a specimen current of 0.2 nA. A spot analysis was performed in a STEM mode for 100 s at a specimen current of 2 nA on the mast cell and basophil granules and other areas of the cells. Histamine was examined by the o-phthalaldehyde method.

Mast cell-nerve fiber interaction: Ultrastructural studies

1990 ◽  
Vol 48 (3) ◽  
pp. 428-429
Author(s):  
E.Y. Chi ◽  
M.L. Su ◽  
Y.T. Tien ◽  
W.R. Henderson
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Substance P ◽  
Nerve Fiber ◽  
Sensory Nerves ◽  
Ultrastructural Studies ◽  
Morphological Studies ◽  
Granulocyte Infiltration ◽  
Intracutaneous Injection ◽  
Recent Attention

Recent attention has been directed to the interaction of the nerve and immune systems. The neuropeptide substance P, a tachykinnin which is a neurotransmitter in the central and peripheral nervous systems produces tissue swelling, augemntation of intersitial fibrin deposition and leukocyte infiltration after intracutaneous injection. There is a direct correlation reported between the extent of mast cell degranulation at the sites of injection and the tissue swelling or granulocyte infiltration. It has previously been demonstrated that antidromic electrical stimulation of sensory nerves induces degranulation of cutaneous mast cells, cutaneous vasodilation and augmented vascular permeability. Morphological studies have documented a close anatiomical association between mast cells and nonmyelinated nerves, that contain substance P and other neuropeptides. However, the presence of mast cells within nerve fasicles has not been previously examined ultrastructurally. In this study, we examined ultrastructurally the distribution of mast cells in the nerve fiber bundles located in the muscular connective tissue of rat tongues (n=20).

Ultrastructural evidence for mast cell-macrophage interrelationships in the dura mater of the rat: Infrequent mast cell-nerve associations

1990 ◽  
Vol 48 (3) ◽  
pp. 352-353
Author(s):  
Ruth V.W. Dimlich
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Dura Mater ◽  
Spatial Relationship ◽  
Potassium Phosphate ◽  
Plasma Extravasation ◽  
Close Spatial Relationship ◽  
Male Wistar Rats ◽  
Headache Pain ◽  
Relationship Of

Mast cells in the dura mater of the rat may play a role in cerebral pathologies including neurogenic inflammation (vasodilation; plasma extravasation) and headache pain . As has been suggested for other tissues, dural mast cells may exhibit a close spatial relationship to nerves. There has been no detailed ultrastructural description of mast cells in this tissue; therefore, the goals of this study were to provide this analysis and to determine the spatial relationship of mast cells to nerves and other components of the dura mater in the rat.Four adult anesthetized male Wistar rats (290-400 g) were fixed by perfusion through the heart with 2% glutaraldehyde and 2.8% paraformaldehyde in a potassium phosphate buffer (pH 7.4) for 30 min. The head of each rat was removed and stored in fixative for a minimum of 24 h at which time the dural coverings were removed and dissected into samples that included the middle meningeal vasculature. Samples were routinely processed and flat embedded in LX 112. Thick (1 um) sections from a minimum of 3 blocks per rat were stained with toluidine blue (0.5% aqueous).

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