Two populations of mast cells on fibroblast monolayers: correlation of quantitative microscopy and functional activity

1988 ◽  
Vol 91 (1) ◽  
pp. 13-19
Author(s):  
I. Hammel ◽  
H. Shiloh-Rabinovich ◽  
I. Nir
Keyword(s):  
Mast Cell ◽  
Lymph Node ◽  
Mast Cells ◽  
Cultured Cells ◽  
Dense Granules ◽  
Cellular Biochemistry ◽  
Lymph Node Cells ◽  
Embryonic Fibroblast ◽  
Two Populations ◽  
Morphometric Evaluation

We have previously shown that a confluent layer of mature mast cells is obtained when lymph node cells are grown on embryonic fibroblast monolayers. Two populations of mast cells may be observed, depending on treatment of the mice from which the lymph node cells are derived. We report now on the morphometric evaluation of these two mast cell entities, and we correlate this with cellular biochemistry and secretory behaviour. The first type of mast cell is small (265 +/− 20 microns 3). It arises from the embryonic monolayer and the cells have feathers resembling those of connective tissue mast cells. These cultured cells are filled with about 1000 homogeneous electron-dense granules, which usually range in diameter from 0.05 to 0.2 microns. The second type of mast cell arises from precursors originating from the lymph node and they have feathers of mucosal mast cells. These cells are larger (480 +/− 40 microns 3) and contain about 300 heterogeneous granules, which range from 0.1 to 0.8 microns. Both cell entities contain about equal amounts of histamine, serotonin and chymase. Biologically, the two cell entities secrete soluble mediators (histamine and serotonin) at different rates compared to the rate at which they secrete chymase. We suggest that such a pattern of secretion exhibits a form of degranulation that permits the release of freely diffusible mediators that are loosely bound to granules, but only partially permits the secretion of insoluble mediators, which are stored in the granules. Alternatively, there might be a mechanism that rapidly inactivates or binds chymase so that only the vasoamines will be free.

scholarly journals MATURATION OF RAT MAST CELLS

1966 ◽  
Vol 31 (3) ◽  
pp. 563-575 ◽  
Author(s):  
J. W. Combs
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Granular Material ◽  
Electron Microscope Study ◽  
Cell Granule ◽  
Cell Maturation ◽  
Granule Formation ◽  
Dense Granules ◽  
Mast Cell Granule ◽  
Rat Mast Cells

Electron microscope study of rat mast cell maturation corroborates certain interpretations of features of mast cell differentiation based on light microscope studies. In addition, the ultrastructural variation observed in the granules of differentiating mast cells suggests that granule formation begins with the elaboration of dense granules about 70 mµ in diameter inside Golgi vacuoles. These progranules appear to aggregate inside a membrane and fuse to form dense cords 70 to 100 mµ in diameter. These dense cords are embedded in a finely granular material possibly added to the developing granule by direct continuity between perigranular membranes and cisternae of rough endoplasmic reticulum. The dense cords and finely granular material then appear to be replaced by a mass of strands about 30 mµ in diameter, thought to be a reorganization product of the two formerly separate components. A process interpreted as compaction of the strands completes the formation of the dense, homogeneous granules observed in mature rat mast cells. The similarity between mast cell granule formation and the elaboration of other granules is considered, with special reference to rabbit polymorphonuclear leukocyte azurophil granules. The relationships between the ultrastructural, histochemical, and radioautographic characteristics of mast cell granule formation are considered, and the significance of the perigranular membrane is discussed.

scholarly journals Intercellular interactions between mast cells and stromal fibroblasts obtained from canine cutaneous mast cell tumours

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Lidia H. Pulz ◽  
Yonara G. Cordeiro ◽  
Greice C. Huete ◽  
Karine G. Cadrobbi ◽  
Arina L. Rochetti ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Cell Viability ◽  
Cultured Cells ◽  
Primary Cultures ◽  
Physical Contact ◽  
Cell Contact ◽  
Low Grade ◽  
Stromal Fibroblasts

AbstractMast cell tumours (MCTs) are the most frequent malignant skin neoplasm in dogs. Due to the difficulty in purifying large numbers of canine neoplastic mast cells, relatively little is known about their properties. A reproducible in vitro model is needed to increase the understanding about the phenotype and functional properties of neoplastic mast cells. In the present study, we describe the establishment of primary cocultures of neoplastic mast cells from canine cutaneous MCTs and cancer-associated fibroblasts. We confirmed the inability of canine neoplastic mast cells to remain viable for long periods in vitro without the addition of growth factors or in vivo passages in mice. Using a transwell system, we observed that mast cell viability was significantly higher when there is cell-to-cell contact in comparison to non-physical contact conditions and that mast cell viability was significantly higher in high-grade than in low-grade derived primary cultures. Moreover, the use of conditioned medium from co-cultured cells led to a significantly higher tumoral mast cell viability when in monoculture. Signalling mechanisms involved in these interactions might be attractive therapeutic targets to block canine MCT progression and deserve more in-depth investigations.

Herpestype Virus and Type C Particles in Spontaneous Equine Lymphoma

1970 ◽  
Vol 28 ◽  
pp. 162-163
Author(s):  
K. Maruyama ◽  
G. R. Swearingen ◽  
L. Dmochowski ◽  
J. H. Jardine ◽  
G. N. Joiner
Keyword(s):  
Lymph Node ◽  
Cultured Cells ◽  
Reticulum Cell ◽  
Cell Type ◽  
Virus Particles ◽  
Lymph Node Cells ◽  
Type C ◽  
Indented Nucleus ◽  
Nuclear Protrusion

Herpestype and type C virus particles are known to produce tumors in animals of certain species, and to be present in a variety of human tissues. Similar particles were found in a horse lymphoma.Electron microscope study was carried out on a tumorous prescapular lymph node from an adult female horse with spontaneous lymphoma. Lymph node cells grown in vitro for a total of 105 days (6th passage) were also studied. The lymph node was infiltrated by blastic cells of reticulum cell type and of lymphoblast type. No virus particles were found in the original lymph node. The cultured cells have a large cytoplasm with well developed rough endoplasmic reticulum and Golgi's apparatus, and an indented nucleus with marginated chromatin. Occasionally, intranuclear fibrous structures were observed. Elongated nuclear protrusion were frequently seen.

scholarly journals Brief Report: Nuclear Bodies of Normal and Pathological Human Lymph Node Cells: An Electron Microscopic Study

Blood ◽  
1967 ◽  
Vol 29 (2) ◽  
pp. 269-275 ◽  
Author(s):  
ROBERT E. BROOKS ◽  
BENJAMIN V. SIEGEL
Keyword(s):  
Electron Microscope ◽  
Lymph Node ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Nuclear Bodies ◽  
Electron Microscopic ◽  
Dense Granules ◽  
Fibrillar Material ◽  
Human Lymph Node ◽  
Lymph Node Cells

Abstract Nuclear bodies in normal and pathologic human lymph node cells have been examined with the electron microscope and their structure has been illustrated and described. In normal lymph node cells, nuclear bodies are 0.3-0.5 microns in diameter, are slightly less electron dense than the nucleolus, and consist of peripheral fibrillar material with centrally located, dense granules, 200-400 Å in diameter. Morphologically abnormal nuclear bodies have been observed in a case of Hodgkin’s disease. The appearance of these atypical bodies would suggest either contact and fusion of two or more atypical bodies, or possibly the existence of single, large, irregular bodies.

Mast cell ultrastructure in the adenoids of children with and without secretory otitis media

1994 ◽  
Vol 108 (12) ◽  
pp. 1058-1063 ◽  
Author(s):  
Adrian Drake-Lee ◽  
Jacqueline Price ◽  
R. Varley
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Otitis Media ◽  
Otitis Media With Effusion ◽  
Cell Ultrastructure ◽  
Secretory Otitis Media ◽  
Recurrent Tonsillitis ◽  
Dense Granules ◽  
Standard Manner ◽  
The Ideal

AbstractThis study was designed to compare the differences in morphology of the mast cells from the adenoid in children with otitis media with effusion (OME) and those from children with recurrent tonsillitis. Tissue for electron microscopy was prepared in the standard manner and between three and 10 blocks were examined for each child. All the mast cells with nuclei were photographed and the condition of the granules noted. The number of electron dense granules in each cell was assessed on a scale between zero and 10. Sixteen unselected children with OME were compared with 19 children with recurrent tonsillitis. There were no obvious differences in the degree of degranulation between the two groups although there was more vacuolation than reviously described in the normal nose but less than in those patients with perennial allergic rhinitis. Allergy and mast cell reactions do not seem to predispose to OME. It was concluded that the adenoids are not the ideal tissue in which to study normal mast cells.

Cytochemical analysis of mast cell granules after poly-dl-lysine action

1978 ◽  
Vol 36 (2) ◽  
pp. 278-279
Author(s):  
R. Courtoy ◽  
L.J. Simar ◽  
J. Christophe
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Chemical Compounds ◽  
Cellular Membrane ◽  
Thin Sections ◽  
Organic Bases ◽  
Ferric Thiocyanate ◽  
Cytochemical Analysis ◽  
Mast Cell Granule ◽  
Amine Liberation

Several chemical compounds induce amine liberation from mast cells but do not necessarily provoque the granule expulsion. For example, poly-dl-lysine induces modifications of the cellular membrane permeability which promotes ion exchange at the level of mast cell granules. Few of them are expulsed but the majority remains in the cytoplasm and appears less dense to the electrons. A cytochemical analysis has been performed to determine the composition of these granules after the polylysine action.We have previously reported that it was possible to demonstrate polyanions on epon thin sections using a cetylpyridinium ferric thiocyanate method. Organic bases are selectively stained with cobalt thiocyanate and the sulfhydryle groups are characterized with a silver methenamine reaction. These techniques permit to reveal the mast cell granule constituents, i.e. heparin, biogenic amines and basic proteins.

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