In vitro effects of the chemotherapy agent water‐soluble micellar paclitaxel (Paccal Vet) on canine hemangiosarcoma cell lines

The objective of this study was to investigate the potential effect of the polysaccharides isolated from Hericium novae-zealandiae, a native New Zealand fungus, on the in vitro proliferation of prostate cancer cell lines, gene expression, acetylcholinesterase (AChE) activity, and oxidation. One water-soluble and two alkali-soluble polysaccharide fractions were isolated from H. novae-zealandiae. The proliferation of the prostate cancer cell lines DU145, LNCaP, and PC3 was evaluated following treatment with these polysaccharide fractions. It was found that the polysaccharides possess anti-proliferative activity on LNCaP and PC3 cells, with a 50% growth inhibition (IC50) value as low as 0.61 mg/mL in LNCaP. Subsequently, it was determined through via RT-qPCR assay that apoptosis was one of the possible mechanisms responsible for the anti-proliferative activity in LNCaP. This was supported by the up-regulation of CASP3, CASP8, and CASP9. An alternative, discovered in PC3, was revealed to be anti-inflammation, which was hinted at by the down-regulation of IL6 and up-regulation of IL24. The polysaccharides also exhibited antioxidant and weak AChE inhibitory activities. This is the first report on the potential health benefits of polysaccharides prepared from the New Zealand fungus, H. novae-zealandiae.

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The effects of HDIL-2 in combination with pazopanib or sunitinib on PBMCs’ function and RCC cells’ apoptosis: Preliminary in vitro data.

Journal of Clinical Oncology ◽

10.1200/jco.2012.30.5_suppl.463 ◽

2012 ◽

Vol 30 (5_suppl) ◽

pp. 463-463

Author(s):

Alaaeldin Shablak

Keyword(s):

Cell Lines ◽

Negative Impact ◽

Significant Proportion ◽

Chromium Release ◽

Healthy Donors ◽

Anticancer Mechanism ◽

In Vitro Effects ◽

Brdu Assay ◽

In Vitro Data

463 Background: Combination of certain immunotherapies with TKI’s has been used with success in the treatment of metastatic renal cell carcinoma (MRCC) patients. Here we evaluate the in vitro effects of HDIL-2 and pazopanib or sunitinib on the PBMCs’s function and RCC cell lines apoptosis. Methods: PBMCs isolated from healthy donors or RCC patients were treated with different HDIL-2/TKI combination and their proliferation was assessed with CFSE labelling assay. Furthermore, using CD107a degranulation or chromium release assays, the function of these treated cells was evaluated. The BRdU assay was used to examine the effects of these drugs combinations on the proliferation of different RCC cell lines. Results: While HDIL2/sunitinib combination did not inhibit the proliferation of various immune cells induced by HDIL-2 alone, combination of HDIL-2/pazopanib did, on the other hand, inhibit these cells proliferation. Interestingly, none of these combinations had a negative impact on the functional properties of these cells. Significant proportion of RCC cell lines treated with pazopanib underwent apoptosis, while the proportions of apoptotic cells post HDIL-2 or sunitinib treatment were not different from the background. Furthermore, the combination of HDIL-2/pazopanib did not inhibit the pazopanib induced RCC apoptosis. Conclusions: These preliminary data showed that combination of HDIL-2 and either pazopanib or sunitinib could recruit different anticancer mechanism that could enhance the treatment efficacy. Further assessment in this direction is warranted.

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In vitro effects of Apixaban on 5 different cancer cell lines

PLoS ONE ◽

10.1371/journal.pone.0185035 ◽

2017 ◽

Vol 12 (10) ◽

pp. e0185035 ◽

Cited By ~ 2

Author(s):

Luigina Guasti ◽

Alessandro Squizzato ◽

Paola Moretto ◽

Davide Vigetti ◽

Walter Ageno ◽

...

Keyword(s):

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

In Vitro Effects

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The in vitro effects of piroxicam and meloxicam on canine cell lines

Journal of Small Animal Practice ◽

10.1111/j.1748-5827.2006.00006.x ◽

2006 ◽

Vol 47 (1) ◽

pp. 14-20 ◽

Cited By ~ 25

Author(s):

C. Knottenbelt ◽

G. Chambers ◽

E. Gault ◽

D. J. Argyle

Keyword(s):

Cell Lines ◽

In Vitro Effects

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OC.07.3 IN VITRO EFFECTS OF MESENCHYMAL STROMAL CELLS (MSC) ON T CELL LINES FROM CROHN'S DISEASE PATIENTS

Digestive and Liver Disease ◽

10.1016/s1590-8658(13)60195-4 ◽

2013 ◽

Vol 45 ◽

pp. S71

Author(s):

R. Ciccocioppo ◽

G.C. Cangemi ◽

E. Betti ◽

A. Gallia ◽

V. Imbesi ◽

...

Keyword(s):

Crohn’S Disease ◽

T Cell ◽

Crohn's Disease ◽

Cell Lines ◽

Mesenchymal Stromal Cells ◽

Stromal Cells ◽

In Vitro Effects ◽

T Cell Lines

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A Novel Fluorescence-Labeled Curcumin Conjugate: Synthesis, Evaluation and Imaging on Human Cell Lines

Current Pharmaceutical Design ◽

10.2174/1381612824666180406103317 ◽

2018 ◽

Vol 24 (16) ◽

pp. 1821-1826 ◽

Cited By ~ 2

Author(s):

Sumbla Sheikh ◽

Alexander Sturzu ◽

Hubert Kalbacher ◽

Thomas Nagele ◽

Christopher Weidenmaier ◽

...

Keyword(s):

Cell Growth ◽

Cell Lines ◽

Human Cell ◽

Cellular Localization ◽

Membrane Damage ◽

Antibacterial Properties ◽

Water Soluble ◽

Osteosarcoma Cell ◽

Human Cell Lines

Curcumin, as the main ingredient of the curcuma spice, has increasingly become the target of scientific research. The turmeric root where the spice is obtained from has been widely used in the traditional medicine. Moreover, scientific studies have found that curcumin has anti-inflammatory, anti-cancer, anti-angiogenic effects as well as antibacterial properties. Recently, curcumin has gathered interest as a potential therapeutic agent in the research on Alzheimer’s disease. A consistent problem in the investigative and therapeutic applications of curcumin is its poor solubility in aqueous solutions. In the present study, we synthesized a conjugate of curcumin, the amino acid lysine and the fluorescent dye fluorescein. This conjugate was soluble in cell culture medium and facilitated the examination of curcumin with fluorescence imaging methods. We studied the cell growth impact of unmodified curcumin on seven different human cell lines and then analyzed the uptake and cellular localization of our curcumin conjugate with confocal laser scanning imaging and flow cytometry on the seven cell lines. We found that unbound curcumin inhibited cell growth in vitro and was not taken up into the cells. The curcumin conjugate was internalized into the cell cytoplasm in a dot-like pattern and cellular uptake correlated with the cell membrane damage which was measured using propidium iodide. The CAL-72 osteosarcoma cell exhibited 3-4fold increased conjugate uptake and a strong uniform fluorescein staining in addition to the dot-like pattern observed in all cell lines. In conclusion, we successfully synthesized a novel water-soluble fluorescent curcumin conjugate which showed a strong preference for CAL-72 osteosarcoma cells in vitro.

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