Optimasi Konsentrasi Kinetin dan Benzyl Amino Purine Pada Kultur Tunas Vanili (Vanilla planifolia)

Vanilla has a potential to be developed through tissue culture techniques to anticipate the limitations of the parent plant as a source of planting material. The in vitro propagation ability of vanilla shoots needs to be controlled with the regulation of Kinetin and Benzyl Amino Purines. The interests of this study are 1) analysis of the response of vanilla explants at several Kinetin concentrations; 2) analysis of the response of vanilla explants at several concentrations of BAP and 3) analysis of the interaction of Kinetin and BAP on the response of vanilla explants to form shoot multiplication. The research was conducted at the Tissue Culture Laboratory Politeknik Negeri Jember from June to December 2020 using a factorial Completely Randomized Design (CRD). Factor 1 was the Kinetin concentration of 0.0, 1.0, 2.0 mg.L-1 and the second factor was the concentration of BAP 0.5, 1.5, 2.5 mg.L-1. The results proved that the fastest shoot multiplication occurred on MS medium + Kinetin 2 mg.L-1 with a mean of 8.7 days after inoculation. The mean number of shoots was 7.6 shoots/explant with the highest average wet weight of 0.9 grams/explant at the addition of BAP 1.5 mg. L-1 at measurement 70 days after inoculation.

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Pengaruh Kombinasi Sitokinin dan Gibberelin terhadap Pemanjangan Tunas Jambu Mete (Anacardium Occidentale L.) secara In Vitro

Jurnal BIOMA ◽

10.21009/bioma10(1).5 ◽

2014 ◽

Vol 10 (1) ◽

pp. 34

Author(s):

Febrina Ariyanti ◽

Christiani Tumilisar ◽

Rossa Yunita

Keyword(s):

Tissue Culture ◽

Economic Value ◽

Shoot Elongation ◽

Anacardium Occidentale ◽

Culture Techniques ◽

Randomized Design ◽

Alternative Techniques ◽

Completely Randomized Design ◽

Tissue Culture Techniques

Abstract Cashew (Anacardium occidentale L.) is a plant with high economic value. Conventional propagation of this plant still has obstacles, so an alternative techniques using tissue culture could be tried. One of the factors that determine the success of tissue culture techniques is the type and concentration of growth regulators was used. Growth regulator which have effect on shoot elongation is a cytokinin and gibberellin, this research tried to investigate the influence of combination cytokinin and gibberelin on in vitro shoot elongation of cashew. This research was conducted at BB-Biogen, Bogor on June-November 2010. The method in this research was to design experimental method with completely randomized design. The result was cytokinin could increase the length of shoots and quantity of shoots very well until 4 cm and quantity of shoot for 5. With the most effective cytokinin is zeatin of 5 mg/l. Key words: Anacardium occidentale L., cytokinin, elongation of shoots, gibberelin

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IN VITRO MULTIPLICATION OF JABON (ANTHOCEPHALUS CADAMBA (ROXB)) ON VARIOUS CONCENTRATION OF BAP AND IAA

AGROLAND The Agricultural Sciences Journal ◽

10.22487/j24077593.2017.v4.i2.9540 ◽

2018 ◽

Vol 4 (2) ◽

pp. 60

Author(s):

Asgar Taiyeb ◽

Baharuddin Baharuddi

Keyword(s):

Tissue Culture ◽

Culture Techniques ◽

In Vitro Multiplication ◽

Supportive Environment ◽

Randomized Design ◽

The Media ◽

Completely Randomized Design ◽

The Difference ◽

Tissue Culture Techniques

One of problems in the Jabon propagation is the availability of seeds both quality and quantity. Tissue culture technology is one of the alternatives that can be used for the supply of Jabonseeds to produce organs of plants (buds, leaves, roots). The success of plant tissue culture techniques is determined by the condition of explants, a supportive environment and the addition of growth regulators are expected to provide a response to the cultured explants. This study aimed to know the effect of Benzyl Amino Purine (BAP) and Indole Acetic Acid (IAA) to in vitro multiplication of Jabon. This research conducted at the Laboratory of Forestry Sciences, Faculty of Forestry, University of Tadulako from March to May 2015. Using a completely randomized design with treatments: 0.1 mg / l IAA + 1 mg / l BAP (JB1), 0.1 mg / l IAA + 1.5 mg / l BAP (JB2), 0.1 mg / l IAA + 2 mg / l BAP ( JB3) and 0.1 mg / l IAA + 2.5 mg / l BAP (JB4). Each treatment was repeated three times to obtain 12 experimental units. The results showed that the difference in response Jabon of treatment tested was the highest number of buds and leaves were in the media added 0.1 mg / l IAA + 1.5 mg / l BAP. Furthermore, the formation of callus obtained in media which added 0.1 mg / l IAA + 1 mg / l BAP.

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The Effect of Kinetin Concentration on Eucalyptus pellita F. Muell Micro Cutting Growth (In Vitro)

Jurnal Agroekoteknologi Tropika Lembab ◽

10.35941/jatl.1.1.2018.1505.34-37 ◽

2019 ◽

Vol 1 (1) ◽

pp. 34

Author(s):

Ridho Dzikrana

Keyword(s):

Leaf Color ◽

Culture Techniques ◽

Eucalyptus Pellita ◽

Randomized Design ◽

Significant Difference ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Single Factor Experiment ◽

Tissue Culture Techniques

Propagation of quality, uniform, and large quantities of Eucalyptus pellita F. Muell seeds as one type of industrial forest plant can be done by tissue culture techniques. The aim of the study was to determine: 1) the effect of kinetin concentration on the growth of E. pellita micro cuttings; 2) kinetin concentration which gives the best effect on the growth of E. pellitamicro cuttings. A single factor experiment, kinetin concentration, was arranged in a Completely Randomized Design (CRD), consisted of five treatments, namely 0; 1; 2; 3; and 4 mg of kinetin L-1 and each treatment was replicated five times. The variables observed consisted of plant height, leaf color, and number of leaves. Data were analyzed by analysis of variance (anova) and if the anova was significantly different, to compare between the two treatment averages, followed by a Least Significant Difference (LSD) test at the level of 5%. The results showed that different kinetin concentrations had different effects on the height of the culture and number of leaves, but the color of the leaves did not show significant different. A concentration of 3 mg of kinetin L-1 gave the best effect on the height cuttings at all observation ages: 7; 14; 21; 28; 35; 42; and 49 days after inoculation (DAI) and number of leaves.

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Aklimatisasi dan Respon Pertumbuhan Mutan Leucaena Leucocephala Varietas Tarramba Teradaptasi Asam

Jurnal Ilmu Nutrisi dan Teknologi Pakan ◽

10.29244/jintp.19.3.66-70 ◽

2021 ◽

Vol 19 (3) ◽

pp. 66-70

Author(s):

Muhklisani ◽

Panca Dewi Manuhara Karti ◽

Iwan Prihantoro

Keyword(s):

Tissue Culture ◽

Leucaena Leucocephala ◽

Culture Techniques ◽

Best Response ◽

Randomized Design ◽

Culture Production ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Mutant Lines ◽

Tissue Culture Techniques

Acclimatization is the final stage of plant propagation in tissue culture techniques that can determine the success of the nursery process. This study aimed to observe the growth response during the acclimatization stage of the acid-adapted of Leucaena leucocephala Tarramba variety, which developed from tissue culture techniques. The design used in this study was a completely randomized design (CRD) with the cultivation of 11 mutant lines from tissue culture, namely M1-M11 (an acid-adapted mutant from tissue culture addition of 1 ppm IBA) and 2 controls (lamtoro broodstock without gamma irradiation) namely K0 (lamtoro broodstock resulting from tissue culture addition of 0 ppm IBA), K1 (breeding lamtoro from tissue culture addition of 1 ppm IBA). The variables observed were the level of plant viability, plant height, and number of leaves. The results showed that the acclimatization of the plant Leucaena leucocephala to tissue culture production on the M3 and M9 mutant lines gave the best response to plant morphological growth up to 5 WAP (weeks after planting). Key words: acclimatization, IBA hormone, Leucaena leucocephala, tissue culture

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PENGARUH NAA DAN IBA TERHADAP PERAKARAN PURWOCENG (Pimpinella pruatjan Molk.) 77V VITRO

Jurnal Penelitian Tanaman Industri ◽

10.21082/jlittri.v11n4.2005.146-151 ◽

2020 ◽

Vol 11 (4) ◽

pp. 146

Author(s):

SITTIFATMAH SYAHID ◽

OTIH ROSTIANA ◽

MIFTAKHUROHMAH MIFTAKHUROHMAH

Keyword(s):

Tissue Culture ◽

Root Induction ◽

Planting Material ◽

Randomized Design ◽

Induction Technique ◽

Medicinal Crops ◽

Culture Performance ◽

Completely Randomized Design ◽

Plant Acclimatization

ABSTRAK Purwoceng (Pimpinella pruatjan Molk.) merupakan tanaman obat langka yang cukup potensial untuk dikembangkan sebagai bahan baku afrodisiak. Untuk mendukung budidaya tanaman ini diperlukan bahan tanaman yang memadai. Perbanyakan in vitro purwoceng untuk memperoleh bahan tanaman secara masal masih dibatasi oleh sulitnya menginduksi akar, yang berakibat rendahnya keberhasilan aklimatisasi di lapangan. Penelitian bertujuan untuk mendapatkan teknik induksi perakaran dengan menggunakan dua jenis auksin (NAA dan IBA) pada berbagai taraf konsentrasi yaitu 0; 0,1; 0,2; 0,4; 0,6; 0,8; 1,0; 1,5 dan 2,0 mg/1. Penelitian ini dilaksanakan di Laboratorium Kultur Jaringan, Balai Penelitian Tanaman Rempah dan Obat, Bogor dari bulan Januari 2003 sarnpai dengan Februari 2004. Rancangan percobaan yang digunakan adalah acak lengkap dengan tiga ulangan. Setiap ulangan terdiri dari tiga tunas. Parameter yang diamati adalah jumlah akar, panjang akar dan jumlah daun layu serta penampakan kultur secara visual. Hasil penelitian menunjukkan bahwa auksin NAA nyata menghasilkan jumlah akar lebih banyak dan lebih panjang dari IBA. Penggunaan NAA 0,8 g/1 merupakan konsentrasi terbaik untuk induksi akar. Tidak ada perbedaan yang nyata dari penggunaan NAA atapun IBAterhadap parameter jumlah daun layu. Kata kunci : Purwoceng, Pimpinella pruatjan Molk, tanaman obat, pengatur tuumbuh , NAA, IBA, induksi akar, in vitro, Bogor ABSTRACT Effect of NAA and IBA on root induction of pruatjan (Pimpinella pruatjan Molk.) in vitro Pruatjan (Pimpinella pruatjan Molk.) is one of endangered species which is potential to be developed as aphrodisiac source. To support pruatjan cultivation , it is needed to prepare the planting material. In vitro propagation of pruatjan is hampered by the difficulty in inducing the normal roots which affect the successful plant acclimatization. The objective of this research was to obtain the root induction technique using two kinds of auxin (NAA and IBA ) at several concentrations i.e : 0; 0.1; 0.2; 0.4; 0.6; 0.8; 1.0; 1.5 and 2.0 mg/1. This experiment was conducted from January 2003 to February 2004 at the Tissue Culture Laboratory of Indonesian Spices and Medicinal Crops Research Institute (ISMECRI) in Bogor. Experiment was designed as a completely randomized design with three replications. Each replication consisted of three shoots. The parameters observed were number of roots, length of roots, number of senessence leaves and culture performance. The result showed that NAA produced the greatest and the longest roots compared to that of DBA. The use of NAA 0,8 mg/1 performed the best treatment to induce roots. The number of senessence leaves was neither affected by NAA nor IBA. Key words: Pruatjan, Pimpinella pruatjan Molk., medicinal plant, growth regulator, NAA, IBA, root induction, in vitro

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Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽

10.30598/a.v1i1.293 ◽

2018 ◽

Vol 1 (1) ◽

Author(s):

S. Tuhuteru ◽

Meity L Hehanussa ◽

Simon H.T Raharjo

Keyword(s):

Culture Medium ◽

Culture Media ◽

Water Concentration ◽

Medium Composition ◽

Coconut Water ◽

Orchid Species ◽

Randomized Design ◽

Wet Weight ◽

Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media. The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids. Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

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DEVELOPMENT OF NEOLAMARCKIA CADAMBA (KELEMPAYAN) TISSUE CULTURE TECHNIQUES FOR SUSTAINABLE SUPPLY OF PLANTING MATERIALS FOR COMMERCIAL PLANTATION

Jurnal Teknologi ◽

10.11113/jt.v77.6725 ◽

2015 ◽

Vol 77 (24) ◽

Cited By ~ 1

Author(s):

Siti Suhaila A. Rahman ◽

Norwati Muhammad ◽

Nor Hasnida Hassan ◽

Haliza Ismail ◽

Nazirah Abdullah ◽

...

Keyword(s):

Tissue Culture ◽

Mass Production ◽

Nodal Segments ◽

Direct Organogenesis ◽

Timber Species ◽

Culture Techniques ◽

Commercial Plantation ◽

Planting Materials ◽

Tissue Culture Techniques

Neolamarckia cadamba (kelempayan) is a multipurpose and fast growing timber species. The tree is grown for timber, paper-making and as ornamental plant. It is reported that its barks and leaves possesed medicinal values and its flowers are used in perfumes. The species is also known to be suitable for plywood, packing case, toys and short-fibred pulp. Therefore, mass production of high quality planting material of N. cadamba is important to support plantation program of this species. Here we presented mass production of N. cadamba through tissue culture techniques. Nodal segments derived from in vitro germinated seeds were used and induced direct organogenesis to produce shoots and roots using MS media (1962) and plant growth regulators (BAP and IBA) that are relatively cheaper than previously used methods. The tissue culture technique of N. cadamba developed may help in ensuring supply of planting materials that are feasible for commercial plantation purposes.

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Seleksi Toleransi Kekeringan In Vitro terhadap Enam Belas Aksesi Tanaman Terung (Solanum melongena L. ) dengan Polietilena Glikol (PEG)

Jurnal Hortikultura Indonesia ◽

10.29244/jhi.6.1.20-28 ◽

2015 ◽

Vol 6 (1) ◽

pp. 20

Author(s):

Erna Sinaga ◽

Megayani Sri Rahayu ◽

Awang Maharijaya

Keyword(s):

Tissue Culture ◽

Drought Tolerance ◽

In Vitro Selection ◽

Solanum Melongena ◽

Survival Percentage ◽

Drought Tolerant ◽

Randomized Design ◽

Number Of Leaves ◽

Completely Randomized Design

ABSTRACTThe objectives of this study were to study the effect of several concentrations of polyethylene glycol (PEG) on the in vitro growth of eggplant, to find the appropriate PEG concentration for in vitro selection to drought tolerance of eggplant and the drought tolerant eggplant accessions. The experiment was conducted at the Laboratory of Tissue Culture, Department of Agronomy and Horticulture, Bogor Agricultural University. The experiment was arranged in a completely randomized design with two factor. The first factor was concentration of PEG (0, 5, 10, and 15%) while the second factor was eggplant accessions (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090). The results showed that the addition of PEG to in vitro media significantly affected the survival percentage, the percentage of callus, developed the bud and the number of leaves of eggplant. Addition of PEG 10 and 15% in media can be used as the drought tolerance selective agent of eggplant in vitro. Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090 were eggplant accessions which might be tolerant to drought.Keywords: in vitro selection, solanaceae, tissue culture, tolerant, drought ABSTRAKPenelitian ini bertujuan untuk mempelajari pengaruh beberapa konsentrasi polietilena glikol (PEG) terhadap pertumbuhan tanaman terung in vitro, mendapatkan konsentrasi PEG yang dapat digunakan untuk seleksi tanaman terung secara in vitro dan nomor terung toleran terhadap cekamankekeringan. Penelitian ini dilaksanakan di laboratorium Kultur Jaringan, Departemen Agronomi dan Hortikultura, Institut Pertanian Bogor. Penelitian ini disusun dalam rancangan acak lengkap dua faktor. Faktor pertama adalah konsentrasi PEG terdiri atas 0, 5, 10, dan 15%. Faktor kedua adalah nomor terung terdiri atas enam belas nomor (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090). Hasil penelitian menunjukkan bahwa penambahan PEG pada media in vitro memberikan pengaruh nyata dan sangat nyata terhadap persentase hidup eksplan, persentase eksplan berkalus, pertambahan tinggi tunas, dan jumlah daun tanaman terung. Media PEG 10 dan 15% merupakan media yang dapat digunakan untuk seleksi kekeringan tanaman terung in vitro. Nomor terung Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090 merupakan nomor-nomor terung yang toleran terhadap cekaman kekeringan.Kata kunci: kultur jaringan, seleksi in vitro, solanaceae, toleran kekeringan

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The effect of NAA and coconut water combination on garlic (Allium sativum L.) tissue culture

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/905/1/012036 ◽

2021 ◽

Vol 905 (1) ◽

pp. 012036

Author(s):

Nandariyah ◽

L S Mahmudah ◽

R B Arniputri ◽

A T Sakya

Keyword(s):

Tissue Culture ◽

Coconut Water ◽

Emergence Time ◽

Shoot Height ◽

Culture Techniques ◽

Randomized Design ◽

Two Factors ◽

Number Of Leaves ◽

Tissue Culture Techniques ◽

Number Of Shoots

Abstract Tissue culture techniques can increase the number of garlic seedlings. The purpose of this research is to determine the effect of NAA and coconut water in increasing the number of garlic seeds. This research used a Completely Randomized Design of two factors. The treatment used is NAA with concentrations of 0 ppm, 0.5 ppm, 1 ppm, 1.5 ppm, and coconut water concentrations of 0%, 10%, 20%. The variables observed were shoot emergence time, root emergence time, number of shoots, number of roots, number of leaves, shoot height, root length, and number of plantlets. The results showed that the addition of coconut water 20% without the addition of NAA in 1 bulb can produce 3.33 planlets and the results of explant propagation in 1 bulb can produce the number of shoots as many as 15.33 shoots. Giving coconut water with concentrations of 10% and 20% can increase the number of leaves, shoot height, and some planlets. The concentration of NAA 0.5 ppm can accelerate the root emergence time on garlic explant.

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Promoting Tuber Formation In Vitro with Benzyl Amino Purine and Paclobutrazol at Different Concentrations

Akta Agrosia ◽

10.31186/aa.22.1.29-35 ◽

2019 ◽

Vol 22 (1) ◽

pp. 29-35

Author(s):

Usman Kris Joko Suharjo ◽

Hasanudin Hasanudin ◽

Tunjung Pamekas ◽

Hesti Pujiwati ◽

Alyi Vanturini

Keyword(s):

Poor Quality ◽

Tuber Formation ◽

Potato Tubers ◽

Seed Sources ◽

Randomized Design ◽

Plant Culture ◽

Wet Weight ◽

Completely Randomized Design ◽

Range Test

A common problem related to the production of potatoes in Indonesia is the use of poor quality seed sources and declining seeds. Plant tissue culture is one of the best methods used for the provision of quality seeds. This study aims to establish the best combination of BAP concentration and the concentration of paclobutrazol in spurring the growth of cuttings and initiation of potato tubers in vitro.This research was conducted in November 2017 until June 2018 at Agronomy Laboratory of Biotechnology Division and Plant Culture Network of Agriculture Faculty of Bengkulu University. The research design used was Completely Randomized Design (CRD) which consist of 18 combination of BAP treatment and Paclobutrazol was repeated 6 times. The concentration of BAP used was 0 mg / l, 5 mg / l, 10 mg / l and the concentration of Paclobutrazol 0 mg / l; 2.5 mg / l; 5 mg / l; 7.5 mg / l; 10 mg / l; 12.5 mg / l. The data of the research were analyzed by F test of 5% level and if there were different between treatments followed by Duncan's Multiple Range Test (DMRT) test of5% level. The results showed that the combination of BAP and Paclobutrazol concentration had significant effect on all observed variables. Treatment of 5 mg / l BAP and 7.5 mg / l Paclobutrazol is the best medium in inducing potato micro tube as indicated by the fastest tuber formation time, highest percentage of productive crops, largest number of tubers per bottle, number of tubers per productive plant, tuber diameter the largest and highest wet weight of tuber per bottle. Keywords : in vitro, BAP, paclobutrazol, Potato

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