A study of regeneration and transformation competence of cotton varieties of kazakh breeding

2017 ◽  
pp. 3-11
Author(s):  
A.O. Rahimzhanova ◽  
◽  
Zh.A. Kubash ◽  
Y.M. Ramankulov ◽  
Sh.A. Manabaeva
Keyword(s):  
Transformation Competence

scholarly journals Entrepreneurship Education Challenges for Green Transformation

2021 ◽  
Vol 11 (1) ◽  
pp. 15
Author(s):  
Tõnis Mets ◽  
Jack Holbrook ◽  
Siim Läänelaid
Keyword(s):  
Conceptual Model ◽  
Education System ◽  
Global Climate ◽  
Entrepreneurship Education ◽  
Sustainable Living ◽  
Climate Agreements ◽  
Competence Framework ◽  
Transformation Competence

Associated with global climate agreements and the European Union’s focus on climate-neutral goals by 2050, the development of Green Transformation competencies in society has become topical. This viewpoint paper proposes a conceptual model for applying Entrepreneurship Education (EE) to designing an integrated transdisciplinary, Green Transformation Competence framework. In line with this, EE is seen as a tool for developing an active, informed, responsible, yet sustainable, living ecosystem-oriented and green orientation of citizens in the education system. Nevertheless, this viewpoint recognises several challenges for further research.

The transformation competence perspective

2008 ◽  
Vol 59 (11) ◽  
pp. 1435-1448 ◽  
Author(s):  
R J Ormerod
Keyword(s):  
Transformation Competence

scholarly journals A v-H-ras-dependent hemopoietic tumor model involving progression from a clonal stage of transformation competence to autocrine interleukin 3 production.

1989 ◽  
Vol 9 (3) ◽  
pp. 1183-1190 ◽  
Author(s):  
A P Nair ◽  
I D Diamantis ◽  
J F Conscience ◽  
V Kindler ◽  
P Hofer ◽  
...  
Keyword(s):  
Latency Period ◽  
Tumor Model ◽  
Rnase A ◽  
Interleukin 3 ◽  
Long Latency ◽  
Tumor Phenotype ◽  
Mast Cell Line ◽  
Mouse Mast Cell ◽  
Transformation Competence

Autocrine interleukin 3 (IL-3)-secreting tumors were generated from an IL-3-dependent mouse mast cell line (PB-3c) after introduction of the v-H-ras oncogene. Tumor progression was characterized by four distinct phenotypes. The first corresponded to immortalized mast cells unresponsive to the oncogenic effect of v-H-ras. The second was expressed in a clonable subpopulation of PB-3c cells and was marked by the competence to form v-H-ras-dependent tumors (immortalized transformation competence). The third was a direct effect of v-H-ras expression on all PB-3c cells and was characterized in vitro by a reduced IL-3 requirement. Upon injection of v-H-ras-expressing, transformation-competent cells into mice, the final, fully malignant phenotype developed with a long latency period and was marked in vitro by independence of exogenous IL-3 and by autocrine IL-3 stimulation. Northern (RNA) blot analysis and an RNase A-T1 protection assay showed that IL-3 production was strictly associated with the tumor phenotype. Two of six tumors showed an alteration at the 5' region of the IL-3 gene. We conclude that v-H-ras required complementation by IL-3 gene rearrangement or an alternate event to generate autocrine mastocytomas.

scholarly journals Identification and Characterization of Helicobacter pylori Genes Essential for Gastric Colonization

2003 ◽  
Vol 197 (7) ◽  
pp. 813-822 ◽  
Author(s):  
Holger Kavermann ◽  
Brendan P. Burns ◽  
Katrin Angermüller ◽  
Stefan Odenbreit ◽  
Wolfgang Fischer ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Bacterial Infections ◽  
Meriones Unguiculatus ◽  
Type Iv ◽  
Gastric Colonization ◽  
Colonization Factors ◽  
Essential Function ◽  
H Pylori ◽  
Transformation Competence

Helicobacter pylori causes one of the most common, chronic bacterial infections and is a primary cause of severe gastric disorders. To unravel the bacterial factors necessary for the process of gastric colonization and pathogenesis, signature tagged mutagenesis (STM) was adapted to H. pylori. The Mongolian gerbil (Meriones unguiculatus) was used as model system to screen a set of 960 STM mutants. This resulted in 47 H. pylori genes, assigned to 9 different functional categories, representing a set of biological functions absolutely essential for gastric colonization, as verified and quantified for many mutants by competition experiments. Identification of previously known colonization factors, such as the urease and motility functions validated this method, but also novel and several hypothetical genes were found. Interestingly, a secreted collagenase, encoded by hp0169, could be identified and functionally verified as a new essential virulence factor for H. pylori stomach colonization. Furthermore, comB4, encoding a putative ATPase being part of a DNA transformation-associated type IV transport system of H. pylori was found to be absolutely essential for colonization, but natural transformation competence was apparently not the essential function. Thus, this first systematic STM application identified a set of previously unknown H. pylori colonization factors and may help to potentiate the development of novel therapies against gastric Helicobacter infections.

scholarly journals Simultaneous Single-Cell Genome and Transcriptome Sequencing of Termite Hindgut Protists Reveals Metabolic and Evolutionary Traits of Their Endosymbionts

2020 ◽  
Author(s):  
Michael E. Stephens ◽  
Jacquelynn Benjamino ◽  
Joerg Graf ◽  
Daniel J. Gage
Keyword(s):  
Carbon Metabolism ◽  
Reticulitermes Flavipes ◽  
Endosymbiotic Bacteria ◽  
Differential Gene ◽  
Cell Genome ◽  
Transformation Competence ◽  
Termite Hindgut ◽  
Protist Species ◽  
Natural Transformation Competence ◽  
Single Cell Genome

Different protist species which colonize the hindguts of wood feeding Reticulitermes termites are associated with endosymbiotic bacteria belonging to the genus Endomicrobium. In this study, we focused on the endosymbionts of three protist species from Reticulitermes flavipes, which included Pyrsonympha vertens, Trichonympha agilis, and Dinenympha species II. Since these protist hosts represented members of difference taxa which colonize different niches within the hindguts of their termite hosts, we investigated if these differences translated to differential gene content and expression in their endosymbionts. Following assembly and comparative genome and transcriptome analyses, we discovered that these endosymbionts differed with respect to possible niche specific traits such carbon metabolism. Our analyses supported that genes related to carbon metabolism were acquired by horizontal gene transfer (HGT) from donor taxa which are present in termite's hindgut community. In addition, our analyses supported that these endosymbionts have retained and expressed several genes related to natural transformation (competence) and recombination. Taken together, the presence of genes acquired by HGT and a putative competence pathway supported that these endosymbionts are not cut-off from gene flow and that competence may be a mechanism by which members of the Endomicrobium can acquire new traits.

scholarly journals Low-Level Pilin Expression Allows for Substantial DNA Transformation Competence in Neisseria gonorrhoeae

2003 ◽  
Vol 71 (11) ◽  
pp. 6279-6291 ◽  
Author(s):  
Cynthia D. Long ◽  
Deborah M. Tobiason ◽  
Matthew P. Lazio ◽  
Kimberly A. Kline ◽  
H. Steven Seifert
Keyword(s):  
Growth Medium ◽  
Posttranslational Modifications ◽  
Regulatory System ◽  
Dependent Manner ◽  
Dna Transformation ◽  
Dna Uptake ◽  
Transformation Competence ◽  
Pilin Protein ◽  
Different Levels ◽  
Pilus Assembly

ABSTRACT The gonococcal pilus is a major virulence factor that has well-established roles in mediating epithelial cell adherence and DNA transformation. Gonococci expressing four gonococcal pilin variants with distinct piliation properties under control of the lac regulatory system were grown in different levels of the inducer isopropyl-β-d-thiogalactopyranoside (IPTG). These pilin variants expressed various levels of pilin message and pilin protein in response to the level of IPTG in the growth medium. Moreover, posttranslational modifications of the variant pilin proteins were detected, including S-pilin production and glycosylation. The ratio of the modified and unmodified pilin forms did not substantially change with different levels of pilin expression, showing that these modifications are not linked to pilin expression levels. DNA transformation competence was also influenced by IPTG levels in the growth medium. Substantial increases in transformation competence over an isogenic, nonpiliated mutant were observed when limited amounts of three of the pilin variants were expressed. Immunoelectron microscopy showed that when limited amounts of pilin are expressed, pili are rare and do not explain the pilin-dependent transformation competence. This pilin-dependent transformation competence required prepilin processing, the outer membrane secretin PilQ, and the twitching-motility-regulating protein PilT. These requirements show that a fully functional pilus assembly apparatus is required for DNA uptake when limited pilin is produced. We conclude that the pilus assembly apparatus functions to import DNA into the bacterial cell in a pilin-dependent manner but that extended pili are not required for transformation competence.

Sign in / Sign up

Export Citation Format

Share Document