scholarly journals Estudio computacional de las relaciones evolutivas de los receptores ionotrópicos NMDA, AMPA y kainato en cuatro especies de primates

2010 ◽  
Vol 15 (3) ◽  
pp. 183
Author(s):  
Francy Johanna Moreno-Pedraza ◽  
Leonardo René Lareo ◽  
Edgar Antonio Reyes-Montaño
Keyword(s):  
Secondary Structure ◽  
Structural Changes ◽  
Evolutionary Relationship ◽  
Terminal Region ◽  
The Other ◽  
Kainate Receptors ◽  
Phosphorylation Sites ◽  
Ionotropic Receptors ◽  
Nr2 Subunits ◽  
Ancestral Node

<p><strong></strong><strong>Objective.</strong> To identify the influence of changes on the secondary structure and evolutionary relationship of NMDA, AMPA and kainate receptors in <em>Homo sapiens</em>,<em> Pan troglodytes</em>, <em>Pongo pygmaeus</em> and <em>Macaca mulatta</em>. <strong>Materials and methods.</strong> We identified 91 sequences for NMDA, AMPA and kainate receptors and analyzed with software for predicting secondary structure, phosphorylation sites, multiple alignments, selection of protein evolution models and phylogenetic prediction. <strong>Results.</strong> We found that subunits GLUR5, NR2A, NR2C and NR3A showed structural changes in the C-terminal region and formation or loss of phosphorylation sites in this zone. Additionally the phylogenetic prediction suggests that the NMDA NR2 subunits are the closest to the ancestral node that gives rise to the other subunits. <strong>Conclusions.</strong> Changes in structure and phosphorylation sites in GLUR5, NR2A, NR2C and NR3A subunits suggest variations in the interaction of the C-terminal region with kinase proteins and with proteins with PDZ domains, which could affect the trafficking and anchoring of the subunits. On the other hand, the phylogenetic prediction suggests that the changes that occurred in the NR2 subunits gave rise to the other subunits of glutamate ionotropic receptors, primarily because the NMDA and particularly the NR2D subunits are the most closely related to the ancestral node that possibly gave rise to the iGluRs.</p> <p><strong>Key words: </strong>glutamate ionotropic receptors, iGluRs, NMDA, NR1, NR2A, NR2C, NR3A, AMPA, GluR5.</p><br />

Conformational Alterations of α2-Antiplasmin Induced by Complex Formation with Plasmin

1979 ◽  
Author(s):  
E.F. Plow ◽  
B. Wiman ◽  
D. Collen
Keyword(s):  
Complex Formation ◽  
Structural Changes ◽  
Limited Proteolysis ◽  
Terminal Region ◽  
The Other ◽  
Antigenic Determinants ◽  
Chemical Analyses ◽  
Large Fragment ◽  
Reactive Site ◽  
Immunodiffusion Analysis

The conformational and structural changes induced in the α2-antiplasmin (AP) molecule by complex formation with plasmin have been analyzed utilizing quantitative radioimmuno-chemical analyses. Complexes prepared in plasmin excess -(PAP-P) and therefore subjected to limited proteolysis and complexes prepared in AP excess (PAP-A) have been compared with free AP. With AP antiserum, PAP-A, PAP-P and AP yielded reactions of complete identity by immunodiffusion analysis. In radioimmunoassay, however, these were clearly distinguished, and four distinct sets of antigenic determinants were delineated. Set I determinants were expressed equivalently by PAP-P, PAP-A and AP and were, therefore, not altered by complex formation. This set was recognized by 90% of the antibodies, and the determinants were all included within a large fragment of Mr 60,000 derived from the NH2-terminal region of AP. The other three sets of determinants were modulated by complex formation. Set II was expressed by PAP-A and AP but not by PAP-P, and these were sensitive to proteolysis by plasmin. Set III determinants were expressed only by AP and were localized to a peptide of Mr 8,000 derived from the COOH-terminal region of AP. Set IV determinants were also present only on AP but were not present in the peptide and required an intact reactive site in AP for expression. Thus, there is evidence for multiple conformational modulations in AP induced by complex formation, and these modulations can be pinpointed to specific loci within the AP molecule.

Bioinformatic Analysis and Characteristics of Glycoprotein L(gL) Encoded by UL1 Gene of Duck Plague Virus

2013 ◽  
Vol 647 ◽  
pp. 250-257
Author(s):  
Ling Jie Zuo ◽  
An Chun Cheng ◽  
Ming Shu Wang
Keyword(s):  
Secondary Structure ◽  
Physicochemical Properties ◽  
Evolutionary Relationship ◽  
Alpha Helix ◽  
Bioinformatic Analysis ◽  
Random Coil ◽  
Phosphorylation Sites ◽  
Duck Plague Virus ◽  
Online Tools ◽  
Glycosylation Sites

Glycoprotein L(gL) is encoded by UL1 gene of duck plague virus (DPV). Through predicting and analyzing the structure and physicochemical properties of DPV gL protein by using some software and online tools to gain more information of DPV gL protein. The phylogenetic tree shows that DPV gL protein has close evolutionary relationship with the genus Simplexvirus. The online analysis of the physicochemical properties demonstrates that the protein has ten potential phosphorylation sites and five potential O-linked glycosylation sites, and without both the signal peptide and the transmembrance region. In addition, the subcellular localization of gL protein largely locates at mitochondrial with 47.8%. The secondary structure results reveal that random coil dominate among secondary structure elements followed by alpha helix, extended strand and β-turn for all sequences. All the data will help a basis for further functional and physiological features study of the DPV gL protein.

scholarly journals Characterization of intron 7 to exon 8 of heat shock protein 90-aa1 (hsp90aa1) gene in dominant brown layer chicken using some bioinformatic tools

2021 ◽  
Vol 78 (2) ◽  
pp. 55
Author(s):  
Young IRIVBOJE ◽  
Adeboye FAFIOLU ◽  
Oluwabusayo IRIVBOJE ◽  
Christian IKEOBI
Keyword(s):  
Heat Stress ◽  
Secondary Structure ◽  
Homology Modelling ◽  
Phosphorylation Site ◽  
Evolutionary Relationship ◽  
Structural Features ◽  
Avian Species ◽  
Phosphorylation Sites ◽  
Post Translational Modification ◽  
Relationship Study

HSP90AA1, an isoform of HSP90 has been characterized to indicate it plays important roles in basic cellular events. It is activated in chicken in response to heat stress. This study was aimed at the computational analysis of the biochemical cum structural features and an evolutionary relationship study on the HSP90AA1 gene in Dominant brown layers (DBL) and some selected avian species using bioinformatics tools. ProtParam for physicochemical properties. Scanprosite for post-translational modification sites, Netphos-3.1 for phosphorylation sites, BDM-PUB program for Ubiquitination sites, PDBSUM for Secondary structure and homology modelling with SWISS-model. The findings revealed that intron 7 and exon 8 of HSP90AA1 protein in DBL had a molecular weight of 24681.19Da and an instability index of 27.60, contains N-myristoylation, Protein-kinase-C-phosphorylation and Tyrosine-kinase-phosphorylation site-2 post-translational modification sites, 4-serine and 2-threonine phosphorylation sites and 12-ubiquitination sites. The evolutionary relationship study found Japanese quail to be in a sister branch close to DBL and chicken. Motifs detected in the avian species revealed the gene to be highly conserved. The secondary structure consisted of 16-helices, 3-sheets and 14-strands. The homology modelling was 87.25% sequence identity with human MC-HSP90-alpha. The study elucidates the components and characteristics of HSP90AA1 in DBL in response to heat stress.

Conformational Alterations of α2-Antiplasmin Induced By Complex Formation With Plasmin

1979 ◽  
Author(s):  
E Plow ◽  
B Wiman ◽  
D Collen
Keyword(s):  
Complex Formation ◽  
Chemical Analysis ◽  
Structural Changes ◽  
Limited Proteolysis ◽  
Terminal Region ◽  
The Other ◽  
Antigenic Determinants ◽  
Large Fragment ◽  
Reactive Site ◽  
Immunodiffusion Analysis

The conformational and structural changes induced in the α2-antiplasmin (AP) molecule by complex formation with plasmin have been analyzed utilizing quantitative radioimmuno-chemical analysis. Complexes prepared in plasmin excess (PAP-P) and therefore subjected to limited proteolysis and complexes prepared in AP excess (PAP-A) have been compared with free AP. With AP antiserum. PAP-A, PAP-P and AP yielded reactions of complete identity by immunodiffusion analysis. In radioimmunoassay, however, these were clearly distinguished, and four distinct sets of antigenic determinants were delineated. Set I determinants were expressed equivalently by PAP-P, PAP-A and AP and were, therefore, not altered by complex formation. This set was recognized by 90% of the antibodies, and the determinants Were all included within a large fragment of Mr 60,000 derived from the NH2-terminal region of AP. The other three sets of determinants were modulated by complex formation. Set II was expressed by PAP-A and AP but not by PAP-P, and these were sensitive to proteolysis by plasmin. Set III determinants were expressed only by AP and were localized to a peptide of Mr 8,000 derived from the COOH-terminal region of AP. Set IV determinants were also present only on AP but were not present in the peptide and required an intact reactive site in AP for expression. Thus, there is evidence for multiple conformational modulations in AP induced by complex formation, and these modulations can be pinpointed to specific loci within the AP molecule.

scholarly journals Changes in Value Orientations in Serbia, 2003–2018 . Patriarchy, Authoritarianism and Nationalism

Südosteuropa ◽  
2020 ◽  
Vol 68 (3) ◽  
pp. 408-431
Author(s):  
Irena Petrović ◽  
Marija Radoman
Keyword(s):  
Structural Changes ◽  
Empirical Studies ◽  
Value Orientations ◽  
The Other ◽  
Social Changes ◽  
Ethnic Nationalism ◽  
Political Situation ◽  
Other Hand ◽  
The Social ◽  
Private And Public

AbstractThe authors analyze the changes in value patterns—patriarchy, authoritarianism and nationalism—in Serbia in the context of the social changes that have marked the postsocialist transformation period. They focus on the extent and intensity of two sub-patterns within each of these three basic value patterns: private and public patriarchy, general and specific authoritarianism, organic (natural) and ethnic nationalism. The conclusions about changes in these value patterns are drawn on the basis of three empirical studies conducted in 2003, 2012, and 2018. They show the prevalence of private patriarchy, general authoritarianism, and organic (natural) nationalism over their counterparts. Private patriarchy has weakened, which is largely to be explained by the significant structural changes in Serbia. On the other hand, support of general authoritarianism and organic (natural) nationalism has been on the rise, which clearly mirrors the unfavorable economic and political situation in the country.

scholarly journals Involvement of loop 5 lysine residues and the N-terminal β-hairpin of the ribotoxin hirsutellin A on its insecticidal activity

2016 ◽  
Vol 397 (2) ◽  
pp. 135-145 ◽  
Author(s):  
Miriam Olombrada ◽  
Lucía García-Ortega ◽  
Javier Lacadena ◽  
Mercedes Oñaderra ◽  
José G. Gavilanes ◽  
...  
Keyword(s):  
Active Sites ◽  
Structural Changes ◽  
The Other ◽  
Spatial Proximity ◽  
Ribonucleolytic Activity ◽  
The Family ◽  
Lysine Residues ◽  
Hirsutella Thompsonii ◽  
Close Spatial Proximity ◽  
High Degree

Abstract Ribotoxins are cytotoxic members of the family of fungal extracellular ribonucleases best represented by RNase T1. They share a high degree of sequence identity and a common structural fold, including the geometric arrangement of their active sites. However, ribotoxins are larger, with a well-defined N-terminal β-hairpin, and display longer and positively charged unstructured loops. These structural differences account for their cytotoxic properties. Unexpectedly, the discovery of hirsutellin A (HtA), a ribotoxin produced by the invertebrate pathogen Hirsutella thompsonii, showed how it was possible to accommodate these features into a shorter amino acid sequence. Examination of HtA N-terminal β-hairpin reveals differences in terms of length, charge, and spatial distribution. Consequently, four different HtA mutants were prepared and characterized. One of them was the result of deleting this hairpin [Δ(8-15)] while the other three affected single Lys residues in its close spatial proximity (K115E, K118E, and K123E). The results obtained support the general conclusion that HtA active site would show a high degree of plasticity, being able to accommodate electrostatic and structural changes not suitable for the other previously known larger ribotoxins, as the variants described here only presented small differences in terms of ribonucleolytic activity and cytotoxicity against cultured insect cells.

Structural differences between repressed and derepressed forms of p60c-src

1989 ◽  
Vol 9 (6) ◽  
pp. 2648-2656
Author(s):  
A MacAuley ◽  
J A Cooper
Keyword(s):  
Kinase Activity ◽  
Kinase Domain ◽  
Terminal Region ◽  
Phosphorylation Sites ◽  
Carboxy Terminus ◽  
Kinase Activation ◽  
Amino Terminal ◽  
Structural Differences ◽  
Carboxy Terminal ◽  
Pronase E

The kinase activity of p60c-src is derepressed by removal of phosphate from Tyr-527, mutation of this residue to Phe, or binding of a carboxy-terminal antibody. We have compared the structures of repressed and active p60c-src, using proteases. All forms of p60c-src are susceptible to proteolysis at the boundary between the amino-terminal region and the kinase domain, but there are several sites elsewhere that are more sensitive to trypsin digestion in repressed than in derepressed forms of p60c-src. The carboxy-terminal tail (containing Tyr-527) is more sensitive to digestion by pronase E and thermolysin when Tyr-527 is not phosphorylated. The kinase domain fragment released with trypsin has kinase activity. Relative to intact p60c-src, the kinase domain fragment shows altered substrate specificity, diminished regulation by the phosphorylated carboxy terminus, and novel phosphorylation sites. The results identify parts of p60c-src that change conformation upon kinase activation and suggest functions for the amino-terminal region.

Plasticity of secondary structure in the N-terminal region of β-dystroglycan

2001 ◽  
Vol 1546 (1) ◽  
pp. 114-121 ◽  
Author(s):  
Alberto Boffi ◽  
Manuela Bozzi ◽  
Francesca Sciandra ◽  
Cristina Woellner ◽  
Maria Giulia Bigotti ◽  
...  
Keyword(s):  
Secondary Structure ◽  
Terminal Region

scholarly journals Relative Earnings and Manpower Allocation in Developing Economies

1969 ◽  
Vol 9 (1) ◽  
pp. 14-34 ◽  
Author(s):  
Lloyd G. Reynolds
Keyword(s):  
Economic Growth ◽  
Subject Matter ◽  
Structural Changes ◽  
Developing Economies ◽  
Developed Countries ◽  
The Other ◽  
Labour Economics ◽  
Economic Behaviour ◽  
Agriculture Industry ◽  
Starting Point

The less developed countries (LDC) present two kinds of challenge to economists. First, they invite us to develop hypotheses about how economic growth begins and about structural changes during the early decades of growth. Second, they provide a fresh terrain on which specialists in particular subject-matter areas can test accepted notions about economic behaviour. For investigations in labour economics, the structure of earnings provides a convenient starting point. (It is best to say "earnings" rather than "wages" because most workers in the LDC's are self-emplqyed.) Analysis of earnings requires an examination of manpower supplies and requirements. This leads into the economics of agriculture, industry, government, and other labour demanding sectors on one side, and into a study of education and other skill-producing agencies, on the other. Thus by starting with the earnings structure, one is led rather directly into the heart of the economy.

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