Immobilization of Magnetic Beads for Microfluidic Immunoassays

2019 ◽  
Author(s):  
Thanh-Qua Nguyen ◽  
Jeongyun Kim ◽  
Daewoong Lee ◽  
Ji-Seob Choi ◽  
Jaeho Park ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Magnetic Beads ◽  
Sample Solution ◽  
Capture Zone ◽  
Microfluidic Chips ◽  
High Reproducibility ◽  
Microfluidic Immunoassay ◽  
Target Molecules ◽  
Through Hole ◽  
Hole Structure

Abstract Employing magnetic beads (MBs) to microfluidic chips has enabled diverse microscale biomedical applications involving isolation of target molecules, such as, separation, and biosensing. In this report a microfluidic immunoassay chip that can temporarily immobilize MBs for the detection of target biomarkers within a sample solution flown through its channels is introduced. A through-hole structure of the MB capture zone and valves that can control the direction of the flow enabled immobilization of MBs with high reproducibility. Controlling immobilization of MBs shows promise for reproducible immunoassay signal detections for the same concentration of biomarker which is crucial for quantification of the assay. In addition, the structure and position of the captured MBs can potentially be optimal for immunoassay performances where immunoassay reagents including the antigen and the detection antibody are flown through the MB captured through hole maximizing contact for high binding efficiencies.

scholarly journals Detection of Immunoglobulin E with a Graphene-Based Field-Effect Transistor Aptasensor

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Yi Lan ◽  
Sidra Farid ◽  
Xenia Meshik ◽  
Ke Xu ◽  
Min Choi ◽  
...  
Keyword(s):  
Field Effect ◽  
Biomedical Applications ◽  
Field Effect Transistor ◽  
High Selectivity ◽  
Immunoglobulin E ◽  
Dna Aptamer ◽  
Conductive Substrate ◽  
Wide Range ◽  
Target Molecules ◽  
Effect Transistor

DNA aptamers have the ability to bind to target molecules with high selectivity and therefore have a wide range of clinical applications. Herein, a graphene substrate functionalized with a DNA aptamer is used to sense immunoglobulin E. The graphene serves as the conductive substrate in this field-effect-transistor-like (FET-like) structure. A voltage probe in an electrolyte is used to sense the presence of IgE as a result of the changes in the charge distribution that occur when an IgE molecule binds to the IgE DNA-based aptamer. Because IgE is an antibody associated with allergic reactions and immune deficiency-related diseases, its detection is of utmost importance for biomedical applications.

Performance improvement of N-doped carbon ORR catalyst via large through-hole structure

2020 ◽  
Vol 31 (33) ◽  
pp. 335717 ◽  
Author(s):  
Min Zhu ◽  
Pu Xie ◽  
Long Fei Fan ◽  
Min Zhi Rong ◽  
Ming Qiu Zhang ◽  
...  
Keyword(s):  
Performance Improvement ◽  
Through Hole ◽  
Hole Structure

Optimization on the Structure of Ceramic Nozzles by FLUENT Simulation

2013 ◽  
Vol 397-400 ◽  
pp. 213-217
Author(s):  
Ming Wei Ding ◽  
Chang Jing Fu ◽  
Si Bei Yin
Keyword(s):  
Cone Angle ◽  
Two Phase ◽  
Abrasive Particles ◽  
Outflow Velocity ◽  
Fluent Simulation ◽  
Fluent Software ◽  
Simulation Results ◽  
Through Hole ◽  
Hole Structure ◽  
Good Agreement

Based on the theory of gas-solid two-phase flow, abrasive flows in ceramic nozzles with different structures are simulated by FLUENT software and the outflow velocity of particles is compared. The results show: the abrasive outflow velocity of ceramic nozzle with cone angle is large than that of ceramic nozzle with through-hole structure, and the distribution of abrasive particles is more uniform for the ceramic nozzle with cone angle. The best entrance cone angle of ceramic nozzle is 10o30o, and the maximum abrasive outflow velocity of the ceramic nozzle with cone angle of 20o is 90.16 m/s. The simulation results have a good agreement with the experimental results.

Synthesis Fe-Ni Alloy Magnetic Nanoparticles for Biomedical Applications

2006 ◽  
Author(s):  
Ming-Fong Tai ◽  
Jong-Kai Hsiao ◽  
Hon-Man Liu ◽  
Shio-Chao Lee ◽  
Shin-Tai Chen
Keyword(s):  
Magnetic Nanoparticles ◽  
Saturation Magnetization ◽  
Mammalian Cells ◽  
Biomedical Applications ◽  
Magnetic Beads ◽  
Combustion Method ◽  
Chemical Precipitation ◽  
Mri Contrast Agent ◽  
Precipitation Method

In this investigation, we synthesize FeNi alloy magnetic nanoparticles (MNPs) by using both chemical precipitation and combustion methods. The FeNi MNPs prepared by combustion method have a rather high saturation magnetization Ms of ∼180 emu/g and a coercivity field Hc of near zero. The functionalized FeNi MNPs which were coated with biocompatible polyethyleneimine (PEI) polymer have also been synthesized. We demonstrated that the PEI coated FeNi MNPs can enter the mammalian cells in vitro and can be used as a magnetic resonance imagine (MRI) contrast agent. The results demonstrated that FeNi MNPs potentially could be applied in the biomedical field. To prepare a higher quality and well controlled Fe-Ni MNPs, we also developed a thermal reflux chemical precipitation method to synthesize FeNi3 alloy MNPs. The precursor chemicals of Fe(acac)3 and Ni(acac)2 in a molecular ratio of 1:3 reacted in octyl ether solvent at the boiling point of solvent (∼300°C) by the thermal reflux process. The 1,2-hexadecandiol and tri-n-octylphosphine oxide (TOPO) were used as reducer and surfactant, respectively. The chemically precipitated FeNi3 MNPs are well dispensed and have well-controlled particle sizes around 10–20 nm with a very narrow size distribution (± 1.2 nm). The highly monodispersive FeNi3 NPs present good uniformity in particle shape and crystallinity on particle surfaces. The MNPs exhibit well soft magnetism with saturation magnetization of ∼61 emu/g and Hc ∼ 0. The functionalized magnetic beads with biocompatible polymer coated on MNPs are also generated completed for biomedical applications.

Fabrication and properties of anodic alumina humidity sensor with through-hole structure

2008 ◽  
Vol 53 (2) ◽  
pp. 183-187 ◽  
Author(s):  
ZhiYuan Ling ◽  
ShuoShuo Chen ◽  
JinChi Wang ◽  
Yi Li
Keyword(s):  
Humidity Sensor ◽  
Anodic Alumina ◽  
Through Hole ◽  
Hole Structure

Three Minutes-Long Electrophoretically Assisted Zeptomolar Microfluidic Immunoassay with Magnetic-Beads Detection

2007 ◽  
Vol 129 (42) ◽  
pp. 12628-12629 ◽  
Author(s):  
Victor N. Morozov ◽  
Stephanie Groves ◽  
Michael J. Turell ◽  
Charles Bailey
Keyword(s):  
Magnetic Beads ◽  
Microfluidic Immunoassay

scholarly journals 3D-Printed Microfluidics and Potential Biomedical Applications

2021 ◽  
Vol 3 ◽  
Author(s):  
Priyanka Prabhakar ◽  
Raj Kumar Sen ◽  
Neeraj Dwivedi ◽  
Raju Khan ◽  
Pratima R. Solanki ◽  
...  
Keyword(s):  
3D Printing ◽  
Biomedical Applications ◽  
Chemical Properties ◽  
Microfluidic Devices ◽  
Microfluidic Chips ◽  
3D Printers ◽  
Broad Variety ◽  
3D Printed ◽  
Diagnostic Technologies ◽  
Printing Techniques

3D printing is a smart additive manufacturing technique that allows the engineering of biomedical devices that are usually difficult to design using conventional methodologies such as machining or molding. Nowadays, 3D-printed microfluidics has gained enormous attention due to their various advantages including fast production, cost-effectiveness, and accurate designing of a range of products even geometrically complex devices. In this review, we focused on the recent significant findings in the field of 3D-printed microfluidic devices for biomedical applications. 3D printers are used as fabrication tools for a broad variety of systems for a range of applications like diagnostic microfluidic chips to detect different analytes, for example, glucose, lactate, and glutamate and the biomarkers related to different clinically relevant diseases, for example, malaria, prostate cancer, and breast cancer. 3D printers can print various materials (inorganic and polymers) with varying density, strength, and chemical properties that provide users with a broad variety of strategic options. In this article, we have discussed potential 3D printing techniques for the fabrication of microfluidic devices that are suitable for biomedical applications. Emerging diagnostic technologies using 3D printing as a method for integrating living cells or biomaterials into 3D printing are also reviewed.

scholarly journals Large-scale fabrication of free-standing and sub-μm PDMS through-hole membranes

Nanoscale ◽  
2018 ◽  
Vol 10 (16) ◽  
pp. 7711-7718 ◽  
Author(s):  
Hai Le-The ◽  
Martijn Tibbe ◽  
Joshua Loessberg-Zahl ◽  
Marciano Palma do Carmo ◽  
Marinke van der Helm ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Large Scale ◽  
Free Standing ◽  
Simple Method ◽  
Through Hole

A robust and simple method was developed for large-scale fabrication of free-standing and sub-μm PDMS through-hole membranes for biomedical applications.

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