In Vivo Monitoring of Osseointegration

2007 ◽  
Author(s):  
Lindsey R. VanSchoiack ◽  
Veronica I. Shubayev ◽  
Robert R. Myers ◽  
James C. Earthman
Keyword(s):  
Fibrous Tissue ◽  
Tissue Formation ◽  
Surgical Implant ◽  
In Vivo Monitoring

The process of osseointegration is the firm anchoring of a surgical implant by the growth of bone around it without fibrous tissue formation at the interface. This process is critical for long-term implant success. The ability to monitor this process in vivo would allow for personalization of loading protocols to increase the rate of implant success overall by ensuring that implants are not over or under loaded during recovery. Accordingly, there is a strong need for an instrument that has the sensitivity to noninvasively measure osseointegration in vivo. One of the objectives of the present study was to assess the performance of an instrumented percussion probe for quantitatively monitoring the osseointegration process.

A Short Review of Experimental Peritoneal Sclerosis: From Mice to Men

2005 ◽  
Vol 28 (2) ◽  
pp. 97-104 ◽  
Author(s):  
L. Gotloib ◽  
V. Wajsbrot ◽  
A. Shostak
Keyword(s):  
Degradation Products ◽  
Pure Water ◽  
Fibrous Tissue ◽  
Short Review ◽  
Glucose Absorption ◽  
Low Ph ◽  
Thick Sheet ◽  
Other Substances

Peritoneal sclerosis has been induced in rodents in vivo by exposing the membrane to a variety of experimental interventions: asbestos, 0.1% chlorexidine, iron dextran, glucose degradation products, AGE deposits derived from uremia per se, sodium hypochlorite, lypopolysaccharide, low pH, pure water, silica or zymosan. With a few exceptions (pure water, chlorhexidine and low pH), the other substances mentioned operate setting out different degrees of oxidative stress. This short review describes several experimental interventions in rodents, aimed at acute exfoliation or long-term, sustained injury of the mesothelial monolayer performed by means of intraperitoneal injections of different oxidant agents. Acute exfoliation induced by deoxycholate resulted in a depopulated monolayer coincident with immediate alteration of the peritoneal permeability, evidenced by increased urea D/P ratio, higher glucose absorption rate, elevated albumin losses in the effluent and significant reduction of the ultrafiltration rate. In the long term (30 days), these manifestations of membrane failure persisted and coincided with substantial peritoneal sclerosis. Peritoneal sclerosis was also induced by IP injections of 0.125% trypsin and 6.6 mM/L solution of formaldehyde. Using the doughnut rat model of mesothelial regeneration, exposure to 4.25% glucose or 7.5% icodextrin solutions severely hampered repopulation of the monolayer, which was replaced by a thick sheet of fibrous tissue. It is concluded that peritoneal sclerosis derives mostly from sustained oxidative injury to the peritoneal membrane. Loss of the mesothelial monolayer is the first step in the chain of events leading to this complication.

scholarly journals In vivo monitoring of tissue regeneration using a ratiometric lysosomal AIE probe

2020 ◽  
Vol 11 (12) ◽  
pp. 3152-3163 ◽  
Author(s):  
Xiujuan Shi ◽  
Neng Yan ◽  
Guangle Niu ◽  
Simon H. P. Sung ◽  
Zhiyang Liu ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Caudal Fin ◽  
Fin Regeneration ◽  
Lysosomal Ph ◽  
In Vivo Monitoring ◽  
Ratiometric Probe ◽  
First Time

An AIE-active ratiometric probe for the first time achieved the long-term quantification of lysosomal pH during the medaka larva's caudal fin regeneration.

Long-term in vivo monitoring of injury induced brain regeneration of the adult zebrafish by using spectral domain optical coherence tomography

2016 ◽  
Vol 14 (8) ◽  
pp. 081702-81705 ◽  
Author(s):  
Jian Zhang Jian Zhang ◽  
Zhi-Wei Zhang Zhi-Wei Zhang ◽  
Wei Ge Wei Ge ◽  
and Zhen Yuan and Zhen Yuan
Keyword(s):  
Optical Coherence Tomography ◽  
Spectral Domain ◽  
Optical Coherence ◽  
Adult Zebrafish ◽  
In Vivo Monitoring ◽  
Brain Regeneration

Long-term ex vivo and in vivo monitoring of tumor progression by using dual luciferases

2016 ◽  
Vol 497 ◽  
pp. 24-26 ◽  
Author(s):  
Naoki Morita ◽  
Sanae Haga ◽  
Yoshihiro Ohmiya ◽  
Michitaka Ozaki
Keyword(s):  
Tumor Progression ◽  
Ex Vivo ◽  
In Vivo Monitoring

Long-term In Vivo Monitoring of Chemicals with Fiber Sensors

2021 ◽  
Vol 3 (1) ◽  
pp. 47-58
Author(s):  
Jiajia Wang ◽  
Liyuan Wang ◽  
Jianyou Feng ◽  
Chengqiang Tang ◽  
Xuemei Sun ◽  
...  
Keyword(s):  
Fiber Sensors ◽  
In Vivo Monitoring

scholarly journals Porous Ti6Al4V Scaffold Directly Fabricated by Sintering: Preparation andIn VivoExperiment

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Xuesong Zhang ◽  
Guoquan Zheng ◽  
Jiaqi Wang ◽  
Yonggang Zhang ◽  
Guoqiang Zhang ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Morphogenetic Proteins ◽  
Bone Ingrowth ◽  
Fibrous Tissue ◽  
Porous Titanium ◽  
Host Bone ◽  
Tissue Formation ◽  
Long Term Stability ◽  
Increase Bone Formation

The interface between the implant and host bone plays a key role in maintaining primary and long-term stability of the implants. Surface modification of implant can enhance bone ingrowth and increase bone formation to create firm osseointegration between the implant and host bone and reduce the risk of implant losing. This paper mainly focuses on the fabricating of 3-dimensiona interconnected porous titanium by sintering of Ti6Al4V powders, which could be processed to the surface of the implant shaft and was integrated with bone morphogenetic proteins (BMPs). The structure and mechanical property of porous Ti6Al4V was observed and tested. Implant shaft with surface of porous titanium was implanted into the femoral medullary cavity of dog after combining with BMPs. The results showed that the structure and elastic modulus of 3D interconnected porous titanium was similar to cancellous bone; porous titanium combined with BMP was found to have large amount of fibrous tissue with fibroblastic cells; bone formation was significantly greater in 6 weeks postoperatively than in 3 weeks after operation. Porous titanium fabricated by powders sintering and combined with BMPs could induce tissue formation and increase bone formation to create firm osseointegration between the implant and host bone.

scholarly journals Detection and Long-Term In Vivo Monitoring of Individual Tumor-Specific T Cell Clones in Patients with Metastatic Melanoma

2007 ◽  
Vol 178 (11) ◽  
pp. 6789-6795 ◽  
Author(s):  
Jaroslav Michalek ◽  
Ivo Kocak ◽  
Vuk Fait ◽  
Jan Zaloudik ◽  
Roman Hajek
Keyword(s):  
T Cell ◽  
Metastatic Melanoma ◽  
T Cell Clones ◽  
In Vivo Monitoring ◽  
Cell Clones

scholarly journals Host Response and Neo-Tissue Development during Resorption of a Fast Degrading Supramolecular Electrospun Arterial Scaffold

2018 ◽  
Vol 5 (3) ◽  
pp. 61 ◽  
Author(s):  
Renee Duijvelshoff ◽  
Nicole van Engeland ◽  
Karen Gabriels ◽  
Serge Söntjens ◽  
Anthal Smits ◽  
...  
Keyword(s):  
Host Response ◽  
Vascular Tissue ◽  
Smooth Muscle Actin ◽  
Tissue Formation ◽  
Tissue Development ◽  
Synthetic Scaffolds ◽  
Scaffold Degradation ◽  
Inflammatory Macrophages

In situ vascular tissue engineering aims to regenerate vessels “at the target site” using synthetic scaffolds that are capable of inducing endogenous regeneration. Critical to the success of this approach is a fine balance between functional neo-tissue formation and scaffold degradation. Circulating immune cells are important regulators of this process as they drive the host response to the scaffold and they play a central role in scaffold resorption. Despite the progress made with synthetic scaffolds, little is known about the host response and neo-tissue development during and after scaffold resorption. In this study, we designed a fast-degrading biodegradable supramolecular scaffold for arterial applications and evaluated this development in vivo. Bisurea-modified polycaprolactone (PCL2000-U4U) was electrospun in tubular scaffolds and shielded by non-degradable expanded polytetrafluoroethylene in order to restrict transmural and transanastomotic cell ingrowth. In addition, this shield prevented graft failure, permitting the study of neo-tissue and host response development after degradation. Scaffolds were implanted in 60 healthy male Lewis rats as an interposition graft into the abdominal aorta and explanted at different time points up to 56 days after implantation to monitor sequential cell infiltration, differentiation, and tissue formation in the scaffold. Endogenous tissue formation started with an acute immune response, followed by a dominant presence of pro-inflammatory macrophages during the first 28 days. Next, a shift towards tissue-producing cells was observed, with a striking increase in α-Smooth Muscle Actin-positive cells and extracellular matrix by day 56. At that time, the scaffold was resorbed and immune markers were low. These results suggest that neo-tissue formation was still in progress, while the host response became quiescent, favoring a regenerative tissue outcome. Future studies should confirm long-term tissue homeostasis, but require the strengthening of the supramolecular scaffold if a non-shielded model will be used.

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