Computational Modeling of Cell Orientation in 3D Micro-Constructs

2013 ◽  
Author(s):  
Christine Obbink-Huizer ◽  
Cees W. J. Oomens ◽  
Sandra Loerakker ◽  
Jasper Foolen ◽  
Carlijn V. C. Bouten ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Computational Modeling ◽  
Computational Model ◽  
In Vitro Model ◽  
Stress Fibers ◽  
Cell Behavior ◽  
Cell Orientation ◽  
Mechanical Environment ◽  
Vitro Model

In many tissue engineering applications it is essential to understand how cells orient under the influence of their mechanical environment. In vitro engineered models are used to investigate the orientation of F-actin stress fibers inside cells. One such in vitro model [1] consists of a mixture of cells, collagen and matrigel, that is constrained by an array of silicone posts (Figure 1). We have recently developed a computational model to describe the orientation of stress fibers in response to their mechanical environment [2]. In the present study, this computational model is extended to 3D and used to simulate cell behavior in the mentioned in vitro model. This improves our understanding of how stress fibers orient in response to the mechanical environment and aids in optimizing the use of the in vitro model.

Use of an in vitro model in tissue engineering to study wound repair and differentiation of blastema tissue from rabbit pinna

2015 ◽  
Vol 51 (7) ◽  
pp. 680-689 ◽  
Author(s):  
Mohammad Reza Hashemzadeh ◽  
Nasser Mahdavi-Shahri ◽  
Ahmad Reza Bahrami ◽  
Masoumeh Kheirabadi ◽  
Fatemeh Naseri ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Repair ◽  
In Vitro Model ◽  
Vitro Model

Optimization of Schwann Cell Adhesion in Response to Shear Stress in an in Vitro Model for Peripheral Nerve Tissue Engineering

2003 ◽  
Vol 9 (2) ◽  
pp. 233-241 ◽  
Author(s):  
Dara Chafik ◽  
David Bear ◽  
Phong Bui ◽  
Arush Patel ◽  
Neil F. Jones ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Shear Stress ◽  
Cell Adhesion ◽  
Schwann Cell ◽  
Peripheral Nerve ◽  
In Vitro Model ◽  
Nerve Tissue ◽  
Nerve Tissue Engineering ◽  
Vitro Model

scholarly journals Curcumin-Loaded Nanoparticles Impair the Pro-Tumor Activity of Acid-Stressed MSC in an In Vitro Model of Osteosarcoma

2021 ◽  
Vol 22 (11) ◽  
pp. 5760
Author(s):  
Gemma Di Di Pompo ◽  
Margherita Cortini ◽  
Roberto Palomba ◽  
Valentina Di Di Francesco ◽  
Elena Bellotti ◽  
...  
Keyword(s):  
Stromal Cells ◽  
In Vitro Model ◽  
Polymeric Nanoparticles ◽  
Biological Fluids ◽  
Curcuma Longa ◽  
Migration And Invasion ◽  
Cell Behavior ◽  
Osteosarcoma Cells ◽  
Vitro Model

In the tumor microenvironment, mesenchymal stromal cells (MSCs) are key modulators of cancer cell behavior in response to several stimuli. Intratumoral acidosis is a metabolic trait of fast-growing tumors that can induce a pro-tumorigenic phenotype in MSCs through the activation of the NF-κB-mediated inflammatory pathway, driving tumor clonogenicity, invasion, and chemoresistance. Recent studies have indicated that curcumin, a natural ingredient extracted from Curcuma longa, acts as an NF-κB inhibitor with anti-inflammatory properties. In this work, highly proliferating osteosarcoma cells were used to study the ability of curcumin to reduce the supportive effect of MSCs when stimulated by acidosis. Due to the poor solubility of curcumin in biological fluids, we used spherical polymeric nanoparticles as carriers (SPN-curc) to optimize its uptake by MSCs. We showed that SPN-curc inhibited the release of inflammatory cytokines (IL6 and IL8) by acidity-stimulated MSCs at a higher extent than by free curcumin. SPN-curc treatment was also successful in blocking tumor stemness, migration, and invasion that were driven by the secretome of acid-stressed MSCs. Overall, these data encourage the use of lipid–polymeric nanoparticles encapsulating NF-κB inhibitors such as curcumin to treat cancers whose progression is stimulated by an activated mesenchymal stroma.

scholarly journals A Preliminary Evaluation of the Pro-Chondrogenic Potential of 3D-Bioprinted Poly(ester Urea) Scaffolds

Polymers ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 1478 ◽  
Author(s):  
Samuel R. Moxon ◽  
Miguel J.S. Ferreira ◽  
Patricia dos Santos ◽  
Bogdan Popa ◽  
Antonio Gloria ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
In Vitro Model ◽  
Type Ii Collagen ◽  
Substrate Stiffness ◽  
Preliminary Evaluation ◽  
Chondrogenic Potential ◽  
Chondrogenic Phenotype ◽  
Vitro Model ◽  
Cell Phenotypes

Degeneration of articular cartilage (AC) is a common healthcare issue that can result in significantly impaired function and mobility for affected patients. The avascular nature of the tissue strongly burdens its regenerative capacity contributing to the development of more serious conditions such as osteoarthritis. Recent advances in bioprinting have prompted the development of alternative tissue engineering therapies for the generation of AC. Particular interest has been dedicated to scaffold-based strategies where 3D substrates are used to guide cellular function and tissue ingrowth. Despite its extensive use in bioprinting, the application of polycaprolactone (PCL) in AC is, however, restricted by properties that inhibit pro-chondrogenic cell phenotypes. This study proposes the use of a new bioprintable poly(ester urea) (PEU) material as an alternative to PCL for the generation of an in vitro model of early chondrogenesis. The polymer was successfully printed into 3D constructs displaying adequate substrate stiffness and increased hydrophilicity compared to PCL. Human chondrocytes cultured on the scaffolds exhibited higher cell viability and improved chondrogenic phenotype with upregulation of genes associated with type II collagen and aggrecan synthesis. Bioprinted PEU scaffolds could, therefore, provide a potential platform for the fabrication of bespoke, pro-chondrogenic tissue engineering constructs.

scholarly journals Tissue engineering in an in vitro model of human cartilage repair

2016 ◽  
Vol 24 ◽  
pp. S169-S170 ◽  
Author(s):  
C. Sanjurjo Rodriguez ◽  
R. Castro Viñuelas ◽  
T. Hermida Gomez ◽  
I. Fuentes Boquete ◽  
F. De Toro Santos ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Repair ◽  
In Vitro Model ◽  
Human Cartilage ◽  
Vitro Model
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