scholarly journals Color calibration of an RGB digital camera for the microscopic observation of highly specular materials

2015 ◽  
Author(s):  
Juan Martínez-García ◽  
Mathieu Hébert ◽  
Alain Trémeau
Keyword(s):  
Microscopic Observation ◽  
Digital Camera ◽  
Color Calibration

scholarly journals APLIKASI MIKROFOTOGRAFI UNTUK MENGEKSPLORASI JENIS-JENIS CYANOPHYTA

2014 ◽  
Vol 1 (2) ◽  
Author(s):  
Widodo Widodo
Keyword(s):  
Microscopic Observation ◽  
Light Microscope ◽  
Size Range ◽  
Digital Camera ◽  
Small Cell ◽  
Eukaryotic Cells ◽  
Plant Systematics ◽  
Microscopy Observation ◽  
Small Cell Size ◽  
Preparation Techniques

Archiving and dissemination of data , techniques, and observations of Cyanophyta required for optimization of succesfully low plant systematics studies. This paper aims to present the preparation techniques and the results of Cyanophyta algae observing with light microscope and digitaly camera. Sources accurate specimen was also informed by the author repeatedly experience in organizing learning plant systematics. Preparation and microscopic observation Cyanophyta consists of preparation of accurate source of specimens , microscopic observation to obtain optimum picture of the object and focus , shooting or recording images using a digital camera. The image files are stored and are equipped with a scale.Results of preparation , observation and shooting showed that cells and colonies all Cyanophyta (Oscillatoria , Anabaena , Nostoc ) has a similar size range that requires preparation to optimize the use of the same light microscop . Cells of Cyanophyta colonies can be seen clearly in the eyepiece magnification of 10x and 40x objective. Less than that magnification, The thalus of Cyanophyta difficult to observe because of the relatively small cell size compared to the size of eukaryotic cells. By shooting with digitaly camera, microscopy observation images can be captured as digital specimen. Observing the digitaly specimen does not change depending on season and time. The digital images can also be analyzed and studied further.

Color Calibration Method Based on Spectrally Reproduced Colors and its Application to XYZ-Type Digital Camera

2011 ◽  
Vol 222 ◽  
pp. 98-101
Author(s):  
M. Kretkowski ◽  
Ryszard Jabłoński ◽  
Y. Shimodaira
Keyword(s):  
Light Source ◽  
Narrow Band ◽  
Digital Camera ◽  
Color Difference ◽  
Calibration Method ◽  
Primary Color ◽  
Color Calibration ◽  
Average Color ◽  
Digitally Controlled

This paper presents the development of a color calibration method based on spectrally reproduced colors produced by multi-primary color narrow-band LED’s. The additive mixture of the primary colors can spectrally reproduce an object color. This approach has been used in our work to overcome the insufficient calibration results obtained by commonly used color targets such as the Macbeth Chart. The method has been tested using a developed prototype of digitally controlled light source calibrated to simulate object color under D65 Daylight standard illumination. The method allows for obtaining of the average color difference for the calibrated XYZ camera of ∆E=0.79 and shows significant improvement in comparison with calibration using standard Macbeth (∆E=1.47). Moreover, we explain the advantages of color calibration with usage of multi-primary light source with regard to the amount of the primary colors.

Electron Microscopic Observation of the Longitudinal Organization of Poly (p-Phenylene Terephthalamide) Fibers

1982 ◽  
Vol 40 ◽  
pp. 680-681
Author(s):  
Li Li-Sheng ◽  
L.F. Allard ◽  
W.C. Bigelow
Keyword(s):  
Electron Microscopy ◽  
Mechanical Properties ◽  
Scanning Electron Microscopy ◽  
Microscopic Observation ◽  
Electron Microscopic Observation ◽  
Electron Microscopic ◽  
Aromatic Polyamides ◽  
Radial Arrangement ◽  
Scanning Electron ◽  
Better Than

The aromatic polyamides form a class of fibers having mechanical properties which are much better than those of aliphatic polyamides. Currently, the accepted morphology of these fibers as proposed by M.G. Dobb, et al. is a radial arrangement of pleated sheets, with the plane of the pleats parallel to the axis of the fiber. We have recently obtained evidence which supports a different morphology of this type of fiber, using ultramicrotomy and ion-thinning techniques to prepare specimens for transmission and scanning electron microscopy.

Qualitative study on “hydropic degeneration” of The hepatocytes in viral hepatitis

1990 ◽  
Vol 48 (3) ◽  
pp. 568-569
Author(s):  
Le Meizhao ◽  
Ye Ming ◽  
Song Xiaoming ◽  
Xu Jiazhang
Keyword(s):  
Qualitative Study ◽  
Viral Hepatitis ◽  
Chronic Active Hepatitis ◽  
Microscopic Observation ◽  
Hydropic Degeneration ◽  
Light Microscopic Observation ◽  
Biopsy Specimens ◽  
Endoplasm Reticulum ◽  
Mixed Fluid ◽  
Peripheral Cytoplasm

“Hydropic degeneration” of the hepatocytes are often found in biopsy of the liver of some kinds of viral hepatitis. Light microscopic observation, compareted with the normal hepatocytes, they are enlarged, sometimes to a marked degree when the term “balloning” degeneration is used. Their cytoplasm rarefied, and show some clearness in the peripheral cytoplasm, so, it causes a hydropic appearance, the cytoplasm around the nuclei is granulated. Up to the present, many studies belive that main ultrastructural chenges of hydropic degeneration of the hepatocytes are results of the RER cristae dilatation with degranulation and disappearance of glycogen granules.The specimens of this study are fixed with the mixed fluid of the osmium acidpotassium of ferricyanide, Epon-812 embed. We have observed 21 cases of biopsy specimens with chronic severe hepatitis and severe chronic active hepatitis, and found that the clear fields in the cytoplasm actually are a accumulating place of massive glycogen. The granules around the nuclei are converging mitochondria, endoplasm reticulum and other organelles.

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