Gold nanoparticles for tumor detection with proton radiography: optimizing sensitivity and determining detection limits

This paper considers the combination of several immunochemical interactions for multiplex immunochromatographic analysis. It was shown that varying the composition of the gold nanoparticles–antibodies conjugate, its concentration and concentration of the hapten–protein conjugate changes both the maximal binding in the test zone and the range of the determined concentrations of the target analyte. Thus, it becomes possible to compensate for differences in the interaction times for the reagents of different specificities that arise when they are combined on one test strip. These regularities were demonstrated for multiplex analysis of three antibiotics. The developed assay has limits of detection of 10, 500, and 10 ng/mL for chloramphenicol, streptomycin, and ampicillin, respectively. Its working ranges for these three analytes are 0.5–6, 16–250, and 0.4–5 ng/mL. The analysis time is 15 min; the accuracy of the photometric determination of antibiotics in the working ranges is up to 6%.

Download Full-text

Analyte-triggered autocatalytic amplification combined with gold nanoparticle probes for colorimetric detection of heavy-metal ions

Chemical Communications ◽

10.1039/c7cc02198d ◽

2017 ◽

Vol 53 (54) ◽

pp. 7477-7480 ◽

Cited By ~ 31

Author(s):

Juanhua Yang ◽

Yun Zhang ◽

Lang Zhang ◽

Huili Wang ◽

Jinfang Nie ◽

...

Keyword(s):

Heavy Metal ◽

Gold Nanoparticles ◽

Metal Ions ◽

Gold Nanoparticle ◽

Heavy Metal Ions ◽

Colorimetric Detection ◽

Detection Limits ◽

Label Free ◽

Nanoparticle Probes ◽

Free Gold

This work reports a new nanosensor based on analyte-triggered autocatalytic amplification and label-free gold nanoparticles for the colorimetric detection of Hg2+, Cu2+and Ag+with detection limits less than 3 nM.

Download Full-text

Evaluation of a Nanocomposite Based on Reduced Graphene Oxide and Gold Nanoparticles as an Electrochemical Platform for Detection of Sulfamethazine

Journal of Composites Science ◽

10.3390/jcs3020059 ◽

2019 ◽

Vol 3 (2) ◽

pp. 59 ◽

Cited By ~ 3

Author(s):

Martin Silva ◽

Ivana Cesarino

Keyword(s):

Gold Nanoparticles ◽

Graphene Oxide ◽

Hydrothermal Method ◽

Reduced Graphene Oxide ◽

Glassy Carbon ◽

Detection Limits ◽

Microwave Assisted ◽

Reduced Graphene ◽

Swine Effluent

A nanocomposite based on reduced graphene oxide (rGO) and gold nanoparticles (AuNPs) was synthesized by the microwave-assisted hydrothermal method and applied in the determination of sulfamethazine (SMZ) in swine effluent using a glassy carbon (GC) electrode. The rGO-AuNPs nanocomposite was characterized morphologically, electrochemically and spectrochemically, showing that rGO was modified with the AuNPs. The GC/rGO-AuNPs electrode was optimized for the determination of SMZ, achieving detection limits of 0.1 μmol L−1. The proposed sensor was successfully applied to the determination of SMZ in synthetic swine effluent samples.

Download Full-text

Rapid and sensitive colorimetric sensing of the insecticide pymetrozine using melamine-modified gold nanoparticles

Analytical Methods ◽

10.1039/c7ay02658g ◽

2018 ◽

Vol 10 (4) ◽

pp. 417-421 ◽

Cited By ~ 6

Author(s):

Jing-yan Kang ◽

Yu-jie Zhang ◽

Xing Li ◽

Chen Dong ◽

Hong-yu Liu ◽

...

Keyword(s):

Gold Nanoparticles ◽

Natural Water ◽

Visual Detection ◽

Detection Limits ◽

High Sensitivity ◽

Food Samples ◽

Colorimetric Sensing ◽

Au Nps

A rapid, selective, visual detection sensor with high sensitivity and low detection limits for the pesticide pymetrozine (PYM) was prepared from melamine-modified gold nanoparticles (M-Au NPs), and shown to be effective in both natural water and food samples.

Download Full-text

Non Labeled Tumor Detection via Polarization and Spectral Properties of Gold Nanoparticles

Imaging and Applied Optics 2014 ◽

10.1364/isa.2014.im3c.4 ◽

2014 ◽

Author(s):

Dror Fixler ◽

Zeev Zalevsky

Keyword(s):

Gold Nanoparticles ◽

Spectral Properties ◽

Tumor Detection

Download Full-text

Improved detection limits of protein optical fiber biosensors coated with gold nanoparticles

Biosensors and Bioelectronics ◽

10.1016/j.bios.2013.08.058 ◽

2014 ◽

Vol 52 ◽

pp. 337-344 ◽

Cited By ~ 75

Author(s):

Sandrine Lepinay ◽

Alyssa Staff ◽

Anatoli Ianoul ◽

Jacques Albert

Keyword(s):

Gold Nanoparticles ◽

Optical Fiber ◽

Detection Limits

Download Full-text

Quantitative parallel EELS spectrum imaging developed as a new tool in AEM

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010013064x ◽

1992 ◽

Vol 50 (2) ◽

pp. 1202-1203

Author(s):

Gianluigi Botton ◽

Gilles L'espérance

Keyword(s):

Statistical Analysis ◽

Spatial Resolution ◽

Personal Computer ◽

Systematic Study ◽

Present Author ◽

Chemical Elements ◽

Detection Limits ◽

Research Groups ◽

Quantitative Performance ◽

Spectrum Imaging

As interest for parallel EELS spectrum imaging grows in laboratories equipped with commercial spectrometers, different approaches were used in recent years by a few research groups in the development of the technique of spectrum imaging as reported in the literature. Either by controlling, with a personal computer both the microsope and the spectrometer or using more powerful workstations interfaced to conventional multichannel analysers with commercially available programs to control the microscope and the spectrometer, spectrum images can now be obtained. Work on the limits of the technique, in terms of the quantitative performance was reported, however, by the present author where a systematic study of artifacts detection limits, statistical errors as a function of desired spatial resolution and range of chemical elements to be studied in a map was carried out The aim of the present paper is to show an application of quantitative parallel EELS spectrum imaging where statistical analysis is performed at each pixel and interpretation is carried out using criteria established from the statistical analysis and variations in composition are analyzed with the help of information retreived from t/γ maps so that artifacts are avoided.

Download Full-text

Analysis of completed commercial semiconductors using EPMA

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100164970 ◽

1996 ◽

Vol 54 ◽

pp. 502-503

Author(s):

R. Packwood ◽

M.W. Phaneuf ◽

V. Weatherall ◽

I. Bassignana

Keyword(s):

Electron Probe Microanalysis ◽

Electron Probe ◽

Semiconductor Industry ◽

Detection Limits ◽

High Technology ◽

Depth Resolution ◽

Analytical Instruments ◽

Wide Range ◽

Numerous Element ◽

Choice Method

The development of specialized analytical instruments such as the SIMS, XPS, ISS etc., all with truly incredible abilities in certain areas, has given rise to the notion that electron probe microanalysis (EPMA) is an old fashioned and rather inadequate technique, and one that is of little or no use in such high technology fields as the semiconductor industry. Whilst it is true that the microprobe does not possess parts-per-billion sensitivity (ppb) or monolayer depth resolution it is also true that many times these extremes of performance are not essential and that a few tens of parts-per-million (ppm) and a few tens of nanometers depth resolution is all that is required. In fact, the microprobe may well be the second choice method for a wide range of analytical problems and even the method of choice for a few.The literature is replete with remarks that suggest the writer is confusing an SEM-EDXS combination with an instrument such as the Cameca SX-50. Even where this confusion does not exist, the literature discusses microprobe detection limits that are seldom stated to be as low as 100 ppm, whereas there are numerous element combinations for which 10-20 ppm is routinely attainable.

Download Full-text