High-throughput pharmocology system HT-PS 100: instrument for automated quantitative analysis of a ligand-receptor interaction based on ligand concentration-dependent functional responses in cells

2000 ◽  
Author(s):  
Ilya Okun ◽  
Gregory V. Kaler ◽  
Michael Otto ◽  
Alex Okun
Keyword(s):  
Quantitative Analysis ◽  
High Throughput ◽  
Ligand Concentration ◽  
Receptor Interaction ◽  
Functional Responses ◽  
In Cells

scholarly journals DNA-Based Herbal Teas’ Authentication: An ITS2 and psbA-trnH Multi-Marker DNA Metabarcoding Approach

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2120
Author(s):  
Jessica Frigerio ◽  
Giulia Agostinetto ◽  
Valerio Mezzasalma ◽  
Fabrizio De De Mattia ◽  
Massimo Labra ◽  
...  
Keyword(s):  
Quality Control ◽  
Quantitative Analysis ◽  
Medicinal Plants ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
The Other ◽  
Plant Component ◽  
Identification Rate ◽  
Dna Metabarcoding ◽  
Therapeutic Properties

Medicinal plants have been widely used in traditional medicine due to their therapeutic properties. Although they are mostly used as herbal infusion and tincture, employment as ingredients of food supplements is increasing. However, fraud and adulteration are widespread issues. In our study, we aimed at evaluating DNA metabarcoding as a tool to identify product composition. In order to accomplish this, we analyzed fifteen commercial products with DNA metabarcoding, using two barcode regions: psbA-trnH and ITS2. Results showed that on average, 70% (44–100) of the declared ingredients have been identified. The ITS2 marker appears to identify more species (n = 60) than psbA-trnH (n = 35), with an ingredients’ identification rate of 52% versus 45%, respectively. Some species are identified only by one marker rather than the other. Additionally, in order to evaluate the quantitative ability of high-throughput sequencing (HTS) to compare the plant component to the corresponding assigned sequences, in the laboratory, we created six mock mixtures of plants starting both from biomass and gDNA. Our analysis also supports the application of DNA metabarcoding for a relative quantitative analysis. These results move towards the application of HTS analysis for studying the composition of herbal teas for medicinal plants’ traceability and quality control.

scholarly journals 1N1336 Spatio-temporal quantitative analysis of molecular dynamics and interactions in cells using multicolor single-molecules imaging(Bioimaging 1,The 49th Annual Meeting of the Biophysical Society of Japan)

2011 ◽  
Vol 51 (supplement) ◽  
pp. S66
Author(s):  
Katsuo Ichinomiya ◽  
Timothy J Stasevich ◽  
Hiroshi Kimura ◽  
Kumiko Sakata-Sogawa ◽  
Makio Tokunaga
Keyword(s):  
Molecular Dynamics ◽  
Quantitative Analysis ◽  
Annual Meeting ◽  
Single Molecules ◽  
Biophysical Society ◽  
Spatio Temporal ◽  
In Cells

Robust and High-Throughput Sample Preparation for (Semi-)Quantitative Analysis of N-Glycosylation Profiles from Plasma Samples

2012 ◽  
pp. 371-385 ◽  
Author(s):  
L. Renee Ruhaak ◽  
Carolin Huhn ◽  
Carolien A. M. Koeleman ◽  
André M. Deelder ◽  
Manfred Wuhrer
Keyword(s):  
Quantitative Analysis ◽  
Sample Preparation ◽  
High Throughput ◽  
Plasma Samples

Towards Deep Learning Approaches for Quantitative Analysis of High-Throughput DLD

2021 ◽  
Author(s):  
Eric Gioe ◽  
Xiaolin Chen ◽  
Jong-Hoon Kim
Keyword(s):  
Deep Learning ◽  
Quantitative Analysis ◽  
High Throughput ◽  
Learning Approaches

scholarly journals High-throughput, Quantitative Analysis of Acrolein-derived DNA Adducts in Human Oral Cells by Immunohistochemistry

2012 ◽  
Vol 60 (11) ◽  
pp. 844-853 ◽  
Author(s):  
Emily J. Greenspan ◽  
Hanjoo Lee ◽  
Marcin Dyba ◽  
Jishen Pan ◽  
Kepher Mekambi ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Dna Adducts ◽  
High Throughput ◽  
Oral Cells

scholarly journals Spotsizer: High-throughput quantitative analysis of microbial growth

BioTechniques ◽  
2016 ◽  
Vol 61 (4) ◽  
Author(s):  
Leanne Bischof ◽  
Martin Převorovský ◽  
Charalampos Rallis ◽  
Daniel C. Jeffares ◽  
Yulia Arzhaeva ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
High Throughput ◽  
Microbial Growth

Imaging and proteomic study of a clickable iridium complex

Metallomics ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 1344-1352 ◽  
Author(s):  
Xiuxiu Wang ◽  
Jingyi Zhang ◽  
Xinyang Zhao ◽  
Wei Wei ◽  
Jing Zhao
Keyword(s):  
Proteomic Analysis ◽  
Click Reaction ◽  
Receptor Interaction ◽  
Iridium Complex ◽  
Quantitative Proteomic Analysis ◽  
Proteomic Study ◽  
In Cells

We synthesized a clickable iridium complex 2-N3 which can be imaged via click reaction in cells. Quantitative proteomic analysis revealed that ECM–receptor interaction pathway was activated and a series of celluar process was affected by 2-N3.

Sphingolipidomics: High-throughput, structure-specific, and quantitative analysis of sphingolipids by liquid chromatography tandem mass spectrometry

Methods ◽  
2005 ◽  
Vol 36 (2) ◽  
pp. 207-224 ◽  
Author(s):  
Alfred H. Merrill Jr. ◽  
M. Cameron Sullards ◽  
Jeremy C. Allegood ◽  
Samuel Kelly ◽  
Elaine Wang
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Tandem Mass Spectrometry ◽  
High Throughput ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass
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