Application of laser tweezers Raman spectroscopy techniques to the monitoring of single cell response to stimuli

2012 ◽  
Author(s):  
James W. Chan ◽  
Rui Liu ◽  
Dennis L. Matthews
Keyword(s):  
Raman Spectroscopy ◽  
Single Cell ◽  
Cell Response ◽  
Laser Tweezers ◽  
Single Cell Response

scholarly journals Evaluation of Escherichia coli Cell Response to Antibiotic Treatment by Use of Raman Spectroscopy with Laser Tweezers

2010 ◽  
Vol 48 (11) ◽  
pp. 4287-4290 ◽  
Author(s):  
T. J. Moritz ◽  
C. R. Polage ◽  
D. S. Taylor ◽  
D. M. Krol ◽  
S. M. Lane ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Raman Spectroscopy ◽  
Antibiotic Treatment ◽  
Cell Response ◽  
Coli Cell ◽  
Laser Tweezers

scholarly journals 2P325 Analyzing the single cell response of macrophage to the antigen stimulation (I) Development of antigen-stimulation and analysis system

2005 ◽  
Vol 45 (supplement) ◽  
pp. S201
Author(s):  
H. Shindou ◽  
K. Orita ◽  
K. Matsumura ◽  
Y. Wakamoto ◽  
H. Ishimoto ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Response ◽  
Antigen Stimulation ◽  
Analysis System ◽  
Single Cell Response

Laser Tweezers Raman Spectroscopy Potential for Studies of Complex Dynamic Cellular Processes: Single Cell Bacterial Lysis

2009 ◽  
Vol 81 (9) ◽  
pp. 3227-3238 ◽  
Author(s):  
De Chen ◽  
L. Shelenkova ◽  
Y. Li ◽  
C. R. Kempf ◽  
A. Sabelnikov
Keyword(s):  
Raman Spectroscopy ◽  
Single Cell ◽  
Laser Tweezers ◽  
Complex Dynamic ◽  
Cellular Processes ◽  
Bacterial Lysis

scholarly journals Single cell response landscape of graded Nodal signaling in zebrafish explants

2021 ◽  
Author(s):  
Tao Cheng ◽  
Yanyi Xing ◽  
Yunfei Li ◽  
Cong Liu ◽  
Ying Huang ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Fate ◽  
Cell Response ◽  
Expression Patterns ◽  
Cell Types ◽  
Nodal Signaling ◽  
Animal Pole ◽  
Fate Decision ◽  
Single Cell Response

Nodal, as a morphogen, plays important roles in cell fate decision, pattern formation and organizer function. But because of the complex context in vivo and technology limitations, systematic studying of genes, cell types and patterns induced by Nodal alone is still missing. Here, by using a relatively simplified model, the zebrafish blastula animal pole explant avoiding additional instructive signals and prepatterns, we constructed a single cell response landscape of graded Nodal signaling, identified 105 Nodal immediate targets and depicted their expression patterns. Our results show that Nodal signaling is sufficient to induce anterior-posterior patterned axial mesoderm and head structure. Surprisingly, the endoderm induced by Nodal alone is mainly the anterior endoderm which gives rise to the pharyngeal pouch only, but not internal organs. Among the 105 Nodal targets, we identified 14 genes carrying varying levels of axis induction capability. Overall, our work provides new insights for understanding of the Nodal function and a valuable resource for future studies of patterning and morphogenesis induced by it.

scholarly journals Real-time single-cell response to stiffness

2010 ◽  
Vol 107 (38) ◽  
pp. 16518-16523 ◽  
Author(s):  
D. Mitrossilis ◽  
J. Fouchard ◽  
D. Pereira ◽  
F. Postic ◽  
A. Richert ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Cell Response ◽  
Single Cell Response
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