scholarly journals Fast nanoparticle rotational and translational diffusion in synovial fluid and hyaluronic acid solutions

2021 ◽  
Vol 7 (27) ◽  
pp. eabf8467
Author(s):  
Mythreyi Unni ◽  
Shehaab Savliwala ◽  
Brittany D. Partain ◽  
Lorena Maldonado-Camargo ◽  
Qingteng Zhang ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Synovial Fluid ◽  
Translational Diffusion ◽  
Effective Medium ◽  
Inorganic Nanoparticles ◽  
Polymer Chains ◽  
Acid Solutions ◽  
Coated Nanoparticles ◽  
Shed Light ◽  
Medium Viscosity

Nanoparticles are under investigation as diagnostic and therapeutic agents for joint diseases, such as osteoarthritis. However, there is incomplete understanding of nanoparticle diffusion in synovial fluid, the fluid inside the joint, which consists of a mixture of the polyelectrolyte hyaluronic acid, proteins, and other components. Here, we show that rotational and translational diffusion of polymer-coated nanoparticles in quiescent synovial fluid and in hyaluronic acid solutions is well described by the Stokes-Einstein relationship, albeit with an effective medium viscosity that is much smaller than the macroscopic low shear viscosity of the fluid. This effective medium viscosity is well described by an equation for the viscosity of dilute polymer chains, where the additional viscous dissipation arises because of the presence of the polymer segments. These results shed light on the diffusive behavior of polymer-coated inorganic nanoparticles in complex and crowded biological environments, such as in the joint.

scholarly journals Features of Hyaluronic Acid Solutions for Intra-articular Introduction and Recent Trends in Their Development (Review)

2020 ◽  
Vol 9 (2) ◽  
pp. 45-54
Author(s):  
Y. Y. Zagorulko ◽  
E. Y. Zagorulko
Keyword(s):  
Molecular Weight ◽  
Hyaluronic Acid ◽  
Synovial Fluid ◽  
Viscoelastic Properties ◽  
Sodium Succinate ◽  
Acid Solutions ◽  
Molecular Weight Range ◽  
Polymer Complexes ◽  
Promising Area ◽  
The Creation

Introduction. The most common way to maintain the viscoelastic properties of synovial fluid is intra-articular administration of hyaluronic acid solutions. Such forms have several features due to the method of administration, the characteristics of the substance, as well as their composition, technology, and packaging. The aim of the work to analyze the features of hyaluronic acid solutions for intra-articular administration, as well as to consider resent trends to their pharmaceutical development.Text. Currently, in Russia, most of these forms are registered as medical devices. Each drug has its characteristics, including the source of the substance, the main molecular weight and the molecular weight range of hyaluronic acid, the structure of the molecule (linear or cross-linked), the method of its chemical modification, concentration, solution volume, dosage, etc. As excipients most often use sodium chloride, water for injection, and phosphate-buffered saline to maintain pH values close to the synovial fluid. Some prostheses contain mannitol as an antioxidant. Combinations of hyaluronic acid with active chondroprotective substances (chondroitin sulfate, sodium succinate) are known. The main type of primary packaging is glass prefilled syringes. The choice of sterilization methods is determined by the chemical structure of hyaluronic acid, aseptic production is used for most prostheses.Conclusion. Currently, research solutions to create thermostable and enzyme-resistant compositions with hyaluronic acid for intra-articular administration are being successfully applied. Modern developments are aimed at creating polymer complexes of hyaluronic acid with substances that improve the lubricity of solutions, the development of nanosystems (liposomes, nanoparticles, nano micelles, etc.) with chondroprotective, as well as the creation of inert biocompatible prostheses with viscoelastic properties. The creation of forms of hyaluronic acid and alternative drugs that can support the rheological properties of synovial fluid is currently a promising area of research.

scholarly journals Hyaluronic Acid in Synovial Fluid

1970 ◽  
Vol 11 (2) ◽  
pp. 139-155 ◽  
Author(s):  
Nils W. Rydell ◽  
Judson Butler ◽  
Endre A. Balazs
Keyword(s):  
Hyaluronic Acid ◽  
Synovial Fluid

scholarly journals The Role of Hyaluronic Acid in Cartilage Boundary Lubrication

Cells ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 1606 ◽  
Author(s):  
Weifeng Lin ◽  
Zhang Liu ◽  
Nir Kampf ◽  
Jacob Klein
Keyword(s):  
Hyaluronic Acid ◽  
Synovial Fluid ◽  
Surface Force ◽  
Force Balance ◽  
New Paradigm ◽  
Low Friction ◽  
Cartilage Surface ◽  
Boundary Lubricant ◽  
Lipid Layers

Hydration lubrication has emerged as a new paradigm for lubrication in aqueous and biological media, accounting especially for the extremely low friction (friction coefficients down to 0.001) of articular cartilage lubrication in joints. Among the ensemble of molecules acting in the joint, phosphatidylcholine (PC) lipids have been proposed as the key molecules forming, in a complex with other molecules including hyaluronic acid (HA), a robust layer on the outer surface of the cartilage. HA, ubiquitous in synovial joints, is not in itself a good boundary lubricant, but binds the PC lipids at the cartilage surface; these, in turn, massively reduce the friction via hydration lubrication at their exposed, highly hydrated phosphocholine headgroups. An important unresolved issue in this scenario is why the free HA molecules in the synovial fluid do not suppress the lubricity by adsorbing simultaneously to the opposing lipid layers, i.e., forming an adhesive, dissipative bridge between them, as they slide past each other during joint articulation. To address this question, we directly examined the friction between two hydrogenated soy PC (HSPC) lipid layers (in the form of liposomes) immersed in HA solution or two palmitoyl–oleoyl PC (POPC) lipid layers across HA–POPC solution using a surface force balance (SFB). The results show, clearly and surprisingly, that HA addition does not affect the outstanding lubrication provided by the PC lipid layers. A possible mechanism indicated by our data that may account for this is that multiple lipid layers form on each cartilage surface, so that the slip plane may move from the midplane between the opposing surfaces, which is bridged by the HA, to an HA-free interface within a multilayer, where hydration lubrication is freely active. Another possibility suggested by our model experiments is that lipids in synovial fluid may complex with HA, thereby inhibiting the HA molecules from adhering to the lipids on the cartilage surfaces.

Concentration of Hyaluronic Acid in Synovial Fluid

1959 ◽  
Vol 5 (5) ◽  
pp. 465-469 ◽  
Author(s):  
Barry Decker ◽  
Warren F McGuckin ◽  
Bernard F McKenzie ◽  
Charles H Slocumb
Keyword(s):  
Hyaluronic Acid ◽  
Synovial Fluid ◽  
Inverse Relationship ◽  
Uronic Acids

Abstract 1. The concentration of hyaluronic acid in synovial fluid was determined by a modification of the Dische carbazole method for the determination of uronic acids. 2. The results of this determination in 80 specimens of normal and abnormal synovial fluid confirmed values reported by other methods. 3. An inverse relationship between the hyaluronic acid in the synovial fluid and the degree of synovitis was suggested.

Spin-Gitter-Relaxationszeit-T1-Untersuchungen an Hyaluronsäure. / Spin-Lattice-Relaxationtime-T1 Measurements of Hyaluronic Acid

1979 ◽  
Vol 34 (7-8) ◽  
pp. 508-511 ◽  
Author(s):  
H. Hofmann ◽  
O. Schmut ◽  
H. Sterk ◽  
H. Kopp
Keyword(s):  
Hyaluronic Acid ◽  
Acid Solutions ◽  
Spin Lattice ◽  
Ph Values

Reductions with [3H]NaBH4 proof that the decrease in viscosity of hyaluronic acid solutions caused by lowering the pH does not depend on a depolymerisation of hyaluronic acid. At the same time investigations at different pH-values, show a sigmoide increase of the Spin-Lattice-Re­laxationtime T1. This increase depends on a progressive aggregation of the hyaluronic acid mole­cule. The effect seems to be induced by the decrease of ionization of the carboxylgroups, by acidi­fication of the solution.

Isolation of Hyaluronic Acid by means of Buffered Trichloroacetic Acid Solutions

Nature ◽  
1952 ◽  
Vol 169 (4290) ◽  
pp. 114-115 ◽  
Author(s):  
W. E. JANCSIK ◽  
E. KAISER
Keyword(s):  
Hyaluronic Acid ◽  
Trichloroacetic Acid ◽  
Acid Solutions

Inflammatory and Noninflammatory Synovial Fluids Exhibit New and Distinct Tribological Endotypes

2020 ◽  
Vol 142 (11) ◽  
Author(s):  
Elizabeth Feeney ◽  
Devis Galesso ◽  
Cynthia Secchieri ◽  
Francesca Oliviero ◽  
Roberta Ramonda ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Hyaluronic Acid ◽  
Synovial Fluid ◽  
Inflammatory Arthritis ◽  
Tribological Behavior ◽  
Friction Coefficients ◽  
Low Concentrations ◽  
Synovial Fluids ◽  
Erratic Behavior ◽  
Lubricating Properties

Abstract Inferior synovial lubrication is a hallmark of osteoarthritis (OA), and synovial fluid (SF) lubrication and composition are variable among OA patients. Hyaluronic acid (HA) viscosupplementation is a widely used therapy for improving SF viscoelasticity and lubrication, but it is unclear how the effectiveness of HA viscosupplements varies with arthritic endotype. The objective of this study was to investigate the effects of the HA viscosupplement, Hymovis®, on the lubricating properties of diseased SF from patients with noninflammatory OA and inflammatory arthritis (IA). The composition (cytokine, HA, and lubricin concentrations) of the SF was measured as well as the mechanical properties (rheology, tribology) of the SF alone and in a 1:1 mixture with the HA viscosupplement. Using rotational rheometry, no difference in SF viscosity was detected between disease types, and the addition of HA significantly increased all fluids' viscosities. In noninflammatory OA SF, friction coefficients followed a typical Stribeck pattern, and their magnitude was decreased by the addition of HA. While some of the IA SF also showed typical Stribeck behavior, a subset showed more erratic behavior with highly variable and larger friction coefficients. Interestingly, this aberrant behavior was not eliminated by the addition of HA, and it was associated with low concentrations of lubricin. Aberrant SF exhibited significantly lower effective viscosities compared to noninflammatory OA and IA SF with typical tribological behavior. Collectively, these results suggest that different endotypes of arthritis exist with respect to lubrication, which may impact the effectiveness of HA viscosupplements in reducing friction.

scholarly journals Hyaluronic Acid-Coated Nanomedicine for Targeted Cancer Therapy

Pharmaceutics ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 301 ◽  
Author(s):  
Kim ◽  
Choi ◽  
Choi ◽  
Park ◽  
Ryu
Keyword(s):  
Hyaluronic Acid ◽  
Cancer Therapy ◽  
Cancer Cells ◽  
Photothermal Therapy ◽  
Colloidal Stability ◽  
Inorganic Nanoparticles ◽  
Cancer Targeting ◽  
Anionic Polymer ◽  
Enhanced Permeability And Retention ◽  
Interaction With Receptors

Hyaluronic acid (HA) has been widely investigated in cancer therapy due to its excellent characteristics. HA, which is a linear anionic polymer, has biocompatibility, biodegradability, non-immunogenicity, non-inflammatory, and non-toxicity properties. Various HA nanomedicines (i.e., micelles, nanogels, and nanoparticles) can be prepared easily using assembly and modification of its functional groups such as carboxy, hydroxy and N-acetyl groups. Nanometer-sized HA nanomedicines can selectively deliver drugs or other molecules into tumor sites via their enhanced permeability and retention (EPR) effect. In addition, HA can interact with overexpressed receptors in cancer cells such as cluster determinant 44 (CD44) and receptor for HA-mediated motility (RHAMM) and be degraded by a family of enzymes called hyaluronidase (HAdase) to release drugs or molecules. By interaction with receptors or degradation by enzymes inside cancer cells, HA nanomedicines allow enhanced targeting cancer therapy. In this article, recent studies about HA nanomedicines in drug delivery systems, photothermal therapy, photodynamic therapy, diagnostics (because of the high biocompatibility), colloidal stability, and cancer targeting are reviewed for strategies using micelles, nanogels, and inorganic nanoparticles.

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