Selection of Sucrose-Dependent Escherichia coli To Obtain Envelope Mutants and Fragile Cultures

Science ◽  
1966 ◽  
Vol 153 (3738) ◽  
pp. 892-894 ◽  
Author(s):  
G. Mangiarotti ◽  
D. Apirion ◽  
D. Schlessinger
Keyword(s):  
Escherichia Coli ◽  
Selection Of

The selection of optimum fermentation condition for improved expression of sumo-il11 in escherichia coli

2015 ◽  
Vol 37 (1se) ◽  
Author(s):  
Nguyen Thi Quy ◽  
Duong Thu Huong ◽  
Dang Thi Ngoc Ha ◽  
Le Thi Thu Hong ◽  
Do Thi Huyen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fermentation Condition ◽  
Selection Of

scholarly journals Selection and co-selection of antibiotic resistances among Escherichia coli by antibiotic use in primary care: An ecological analysis

PLoS ONE ◽  
2019 ◽  
Vol 14 (6) ◽  
pp. e0218134 ◽  
Author(s):  
Koen B. Pouwels ◽  
Berit Muller-Pebody ◽  
Timo Smieszek ◽  
Susan Hopkins ◽  
Julie V. Robotham
Keyword(s):  
Escherichia Coli ◽  
Primary Care ◽  
Antibiotic Use ◽  
Ecological Analysis ◽  
Selection Of

scholarly journals Comparison of Common Enrichment Broths Used in Diagnostic Laboratories for Shiga Toxin—Producing Escherichia coli

2021 ◽  
Vol 9 (3) ◽  
pp. 503
Author(s):  
Michael Bording-Jorgensen ◽  
Hannah Tyrrell ◽  
Colin Lloyd ◽  
Linda Chui
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Real Time Pcr ◽  
Acute Gastroenteritis ◽  
Diagnostic Testing ◽  
First Line ◽  
Gram Negative ◽  
Culture Independent ◽  
Surveillance Programs ◽  
Selection Of

Acute gastroenteritis caused by Shiga toxin-producing Escherichia coli (STEC) affects more than 4 million individuals in Canada. Diagnostic laboratories are shifting towards culture-independent diagnostic testing; however, recovery of STEC remains an important aspect of surveillance programs. The objective of this study was to compare common broth media used for the enrichment of STEC. Clinical isolates including O157:H7 as well as non-O157 serotypes were cultured in tryptic soy (TSB), MacConkey (Mac), and Gram-negative (GN) broths and growth was compared using culture on sheep’s blood agar and real-time PCR (qPCR). In addition, a selection of the same isolates was spiked into negative stool and enriched in the same three broths, which were then evaluated using culture on CHROMagarTM STEC agar and qPCR. TSB was found to provide the optimal enrichment for growth of isolates with and without stool. The results from this study suggest that diagnostic laboratories may benefit from enriching STEC samples in TSB as a first line enrichment instead of GN or Mac.

scholarly journals Use of tellurite for the selection of verocytotoxigenic Escherichia coli O157

1993 ◽  
Vol 39 (2) ◽  
pp. 155-158 ◽  
Author(s):  
P. M. Zadik ◽  
P. A. Chapman ◽  
C. A. Siddons
Keyword(s):  
Escherichia Coli ◽  
Escherichia Coli O157 ◽  
Selection Of

Selection of Swine Resistant to F4-Positive Escherichia coli

1997 ◽  
pp. 427-429
Author(s):  
A. Bertels ◽  
J. Jourquin ◽  
N. Buys ◽  
A. Van Zeveren ◽  
H. De Greve ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Selection Of

Attachment of Shiga Toxigenic Escherichia coli to Beef Muscle and Adipose Tissue

2006 ◽  
Vol 69 (5) ◽  
pp. 999-1006 ◽  
Author(s):  
LUCIA RIVAS ◽  
GARY A. DYKES ◽  
NARELLE FEGAN
Keyword(s):  
Escherichia Coli ◽  
Adipose Tissue ◽  
Foodborne Pathogens ◽  
Gastrointestinal Disease ◽  
Attached Bacteria ◽  
The Mean ◽  
Bacterial Hydrophobicity ◽  
Adipose Tissue Cells ◽  
Selection Of ◽  
Beef Muscle

Shiga toxigenic Escherichia coli (STEC) serotypes are important foodborne pathogens that cause gastrointestinal disease worldwide. An understanding of how STEC strains attach to surfaces may provide insight into the potential persistence of and contamination with STEC in food environments. The initial attachment of a selection of STEC serotypes to beef muscle and adipose tissue was evaluated for isolates grown in planktonic and sessile culture. Initial experiments were performed to determine whether attachment differed among STEC strains and between the two modes of growth. Viable counts were obtained for loosely and strongly attached cells, and the strength of attachment (Sr) was calculated. All bacterial isolates grown in sessile culture attached in higher numbers to muscle and adipose tissue than did bacteria in planktonic cultures. For all attachment assays performed, mean concentrations for loosely attached cells were consistently higher than concentrations for strongly attached cells. The mean concentrations for strongly attached bacteria for planktonic and sessile cultures were significantly higher (P < 0.05) on adipose than on muscle tissue. However, some strains of STEC, particularly those from sessile culture, did not differ in their attachment to muscle or adipose tissue. Sr values were not significantly different (P > 0.05) among STEC isolates for all assays. No correlation was found between bacterial hydrophobicity and surface charge values (previously determined) and production of surface structures, viable counts, and Sr values. STEC grown in planktonic and sessile culture seems to behave differently with respect to attachment to muscle and adipose tissue. Cells in sessile culture may have a greater potential to strongly attach to meat surfaces.

scholarly journals Effect of Dimethyl Sulphoxide on the Expression of Nitrogen Fixation in Bacteria

1977 ◽  
Vol 30 (2) ◽  
pp. 141 ◽  
Author(s):  
Mary L Skotnicki ◽  
Barry G Rolfe
Keyword(s):  
Escherichia Coli ◽  
Nitrogen Fixation ◽  
Energy Metabolism ◽  
Membrane Proteins ◽  
Klebsiella Pneumoniae ◽  
Dimethyl Sulphoxide ◽  
Rhizobium Trifolii ◽  
Nif Genes ◽  
E Coli ◽  
Selection Of

Storage in dimethyl sulphoxide (DMSO) of Escherichia coli K12 hybrids carrying nif+ genes from Klebsiella pneumoniae can result in selection of a defective nitrogen-fixing phenotype. Similar results are obtained with E. coli K12 hybrids containing the nitrogep-fixing capacity from Rhizobium trifolii. DMSO appears to affect particular inner membrane proteins associated with energy metabolism in E. coli K12 and four chromosomal regions (chID, chlG, his and unc) are associated with resistance to DMSO.

scholarly journals In VivoTransmission of an IncA/C Plasmid in Escherichia coli Depends on Tetracycline Concentration, and Acquisition of the Plasmid Results in a Variable Cost of Fitness

2015 ◽  
Vol 81 (10) ◽  
pp. 3561-3570 ◽  
Author(s):  
Timothy J. Johnson ◽  
Randall S. Singer ◽  
Richard E. Isaacson ◽  
Jessica L. Danzeisen ◽  
Kevin Lang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
High Dose ◽  
Broad Host Range ◽  
Antibiotic Administration ◽  
Dose Administration ◽  
Content Type ◽  
E Coli ◽  
The Impact ◽  
Selection Of

ABSTRACTIncA/C plasmids are broad-host-range plasmids enabling multidrug resistance that have emerged worldwide among bacterial pathogens of humans and animals. Although antibiotic usage is suspected to be a driving force in the emergence of such strains, few studies have examined the impact of different types of antibiotic administration on the selection of plasmid-containing multidrug resistant isolates. In this study, chlortetracycline treatment at different concentrations in pig feed was examined for its impact on selection and dissemination of an IncA/C plasmid introduced orally via a commensalEscherichia colihost. Continuous low-dose administration of chlortetracycline at 50 g per ton had no observable impact on the proportions of IncA/C plasmid-containingE. colifrom pig feces over the course of 35 days. In contrast, high-dose administration of chlortetracycline at 350 g per ton significantly increased IncA/C plasmid-containingE. coliin pig feces (P< 0.001) and increased movement of the IncA/C plasmid to other indigenousE. colihosts. There was no evidence of conjugal transfer of the IncA/C plasmid to bacterial species other thanE. coli.In vitrocompetition assays demonstrated that bacterial host background substantially impacted the cost of IncA/C plasmid carriage inE. coliandSalmonella.In vitrotransfer and selection experiments demonstrated that tetracycline at 32 μg/ml was necessary to enhance IncA/C plasmid conjugative transfer, while subinhibitory concentrations of tetracyclinein vitrostrongly selected for IncA/C plasmid-containingE. coli. Together, these experiments improve our knowledge on the impact of differing concentrations of tetracycline on the selection of IncA/C-type plasmids.

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