THE DETERMINATION OF THE TOTAL D-AMINO ACID CONTENT OF HUMAN TUMORS AND NORMAL TISSUES BY MEANS OF D-AMINO ACID OXIDASE

Science ◽  
1940 ◽  
Vol 91 (2349) ◽  
pp. 21-23 ◽  
Author(s):  
F. LIPMANN ◽  
O. K. BEHRENS ◽  
E. A. KABIT ◽  
D. BURK
Keyword(s):  
Amino Acid ◽  
Acid Content ◽  
Amino Acid Content ◽  
Human Tumors ◽  
Acid Oxidase ◽  
Amino Acid Oxidase ◽  
Normal Tissues

The Oocytes of the Goby Pomatoschistus minutus. III. Determination of Amino Acid Component

1980 ◽  
Vol 35 (11-12) ◽  
pp. 1094-1095
Author(s):  
Rüdiger Riehl
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Acid Content ◽  
Amino Acid Content ◽  
Acid Component ◽  
Pomatoschistus Minutus ◽  
Protein Amino Acids ◽  
Amino Acid Component

Abstract The oocytes of the marine goby Pomatoschistus minutus were analyzed for their amino acid content. Most of the amino acids exist as protein, only a little part is free or peptide-bound. Among the protein-bound amino acids, high levels of glutamic acid, proline, alanine, aspartic acid, valine and leucine were detected. These represent more than 60% of the protein amino acids. Among the free acids, glutamic acid, serine and alanine, are dominant. There are no certain proofs of the occurrence of peptide pools in the oocytes of Pomatoschistus minutus.

An Automated Detection System for Most L-α-Amino Acids

1969 ◽  
Vol 15 (2) ◽  
pp. 154-161 ◽  
Author(s):  
K Van Dyke ◽  
C Szustkiewicz
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Detection System ◽  
Automated System ◽  
Acid Oxidase ◽  
Enzymatic Reactions ◽  
Amino Acid Oxidase ◽  
Automated Method ◽  
Wide Range

Abstract An automated system for the determination of the L-α form of the majority of amino acids is presented. The method is based upon oxidative deamination of the amino acid coupled with oxidation of o-dianisidine by hydrogen peroxide. This procedure can be used comparatively for the determination of a mixture of L-α-amino acids or for the majority of separated L-α-amino acids (especially in conjunction with column separations from urine and blood which give falsely positive identification with ninhydrin detection). The stereospecific nature of the L-α-amino acid oxidase enables the investigator to quantitate the amount of L-α-amino acid in the presence of the D-α form. From an academic viewpoint, the extreme sensitivity and wide range of the detection system make it advantageous for the study of the enzyme itself. This automated method also may be employed to follow enzymatic reactions—e.g., those catalyzed by peptidases or racemases. The methodology is extremely convenient with good reagent stability and is much more sensitive than manometric technics.

Determination of Free -Proline and -Leucine in the Brains of Mutant Mice Lacking -Amino Acid Oxidase Activity

2001 ◽  
Vol 298 (2) ◽  
pp. 253-258 ◽  
Author(s):  
Kenji Hamase ◽  
Tomomi Inoue ◽  
Akiko Morikawa ◽  
Ryuichi Konno ◽  
Kiyoshi Zaitsu
Keyword(s):  
Amino Acid ◽  
Oxidase Activity ◽  
Mutant Mice ◽  
Acid Oxidase ◽  
Free Proline ◽  
Amino Acid Oxidase

Simultaneous two-dimensional HPLC determination of free d-serine and d-alanine in the brain and periphery of mutant rats lacking d-amino-acid oxidase

2011 ◽  
Vol 879 (29) ◽  
pp. 3184-3189 ◽  
Author(s):  
Yurika Miyoshi ◽  
Kenji Hamase ◽  
Tadashi Okamura ◽  
Ryuichi Konno ◽  
Noriyuki Kasai ◽  
...  
Keyword(s):  
Amino Acid ◽  
Hplc Determination ◽  
Acid Oxidase ◽  
Two Dimensional ◽  
Amino Acid Oxidase ◽  
The Brain
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